RESUMO
Four undescribed ginkgolides, including two rare sesquiterpene ginkgolides (compounds 1 and 2) and two diterpenoid ginkgolides (compounds 3 and 4), were isolated from Ginkgo biloba L. The structures of these four ginkgolides were identified based on extensive spectroscopic analysis, DP4+ probability analysis and X-ray diffraction. Compounds 1 and 2 exhibited excellent antiplatelet aggregation activities with IC50 values of 1.20 ± 0.25 and 4.11 ± 0.34 µM, respectively.
Assuntos
Ginkgo biloba , Ginkgolídeos , Compostos Fitoquímicos , Inibidores da Agregação Plaquetária , Ginkgo biloba/química , Estrutura Molecular , Ginkgolídeos/farmacologia , Ginkgolídeos/isolamento & purificação , Ginkgolídeos/química , Inibidores da Agregação Plaquetária/farmacologia , Inibidores da Agregação Plaquetária/isolamento & purificação , Inibidores da Agregação Plaquetária/química , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/isolamento & purificação , Animais , Agregação Plaquetária/efeitos dos fármacosRESUMO
Ginkgo biloba L. has always been a popular area of research due to its various active ingredients and pharmacological effects. Ginkgo biloba is rich in ginkgo flavonoids, ginkgolides, and ginkgolic acid, with anti-inflammation, antioxidation, neuroprotection, anti-platelet agglutination, hypolipidemic effect, anti-cancer, and anti-radiation properties. There are many methods to extract and separate the active components of ginkgo. Among them, supercritical carbon dioxide fluid extraction (SFE-CO2) is known for its green, clean, and environment-friendly properties. In this paper, the pharmacological activities, the active components, and structures of different parts of ginkgo, the extraction methods of its effective ingredients, and the application of the SFE-CO2 method for the extraction and separation of active ingredients in Ginkgo biloba from leaves, seeds, pollen, and roots were reviewed, in order to make best use of ginkgo resources, and provide support and references for the development of SFE-CO2 of active components from Ginkgo biloba.
Assuntos
Dióxido de Carbono/química , Ginkgo biloba/química , Ginkgolídeos , Extratos Vegetais/química , Folhas de Planta/química , Ginkgolídeos/química , Ginkgolídeos/isolamento & purificaçãoRESUMO
A new bilobalide isomer (1), together with two flavonol glycosides (2, 3), have been isolated and elucidated from the extract of Ginkgo biloba leaves. Significantly, 1 was a new sesquiterpene lactone with two lactone ring groups, both 2 and 3 were two flavonol glycosides with a same cis-coumaroylated fragment. Their chemical structures were elucidated by NMR and MS spectroscopic date and the absolute configuration of 1 was specific established by Cu-Kα X-ray crystallographic analyses. However, 1-3 showed no obvious anti-platelet aggregation activity.
Assuntos
Bilobalídeos/isolamento & purificação , Flavonóis/isolamento & purificação , Ginkgo biloba/química , Glicosídeos/isolamento & purificação , Bilobalídeos/química , Ciclopentanos/química , Ciclopentanos/isolamento & purificação , Flavonóis/química , Furanos/química , Furanos/isolamento & purificação , Ginkgolídeos/química , Ginkgolídeos/isolamento & purificação , Glicosídeos/química , Folhas de Planta/químicaRESUMO
Screening of bioactive ligands for a certain protein target from medicinal herbs is a highly important yet challenging task during drug discovery process. In this study, a surface plasmon resonance biosensor-based active ingredient recognition system (SPR-AIRS) was applied to screen p38 mitogen-activated protein kinase (p38) ligands from herbal extracts. After p38 protein was immobilized on a SPR chip and the suitability of SPR-AIRS was validated, thirty-four p38-related medicinal herbs were selected and pre-screened. Two medicinal herbs having high response signal with p38-immobilized chip, Folium Ginkgo and Herba Artemisiae Scopariae, were injected into SPR system for ligand fishing. Among them, two active compounds, eupatilin (EPT) and ginkgolide B (GKB), were identified as p38 ligands, and then the KD values of EPT and GKB were measured as 21.68 ± 2.21 and 44.71 ± 1.80 µM, respectively. They can inhibit p38 activities significantly and bind to the ATP binding site on p38. Furthermore, EPT and GKB can inhibit cell proliferation (IC50 = 30.31 ± 6.84 and 42.97 ± 0.83 µM), induce apoptosis and G2/M cell cycle arrest against K562 cell line. This is the first time that EPT and GKB are reported as effective p38 binding ligands. These results prove that SPR-AIRS could be an effective method to screen active compounds acting on a specific protein from complex systems.
Assuntos
Artemisia/química , Flavonoides/isolamento & purificação , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Lactonas/isolamento & purificação , Ressonância de Plasmônio de Superfície/métodos , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores , Ligação Competitiva , Técnicas de Cultura de Células , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Flavonoides/farmacologia , Ginkgolídeos/farmacologia , Humanos , Células K562 , Lactonas/farmacologia , Ligantes , Ligação ProteicaRESUMO
The ginkgo terpene lactones (GTL), mainly including bilobalide (BB), ginkgolide A (GA), ginkgolide B (GB) and ginkgolide C (GC) possess different biological activities such as peripheral vasoregulation, platelet-activating factor (PAF) receptor antagonism, neuroprotective properties and prevention of membrane damage caused by free radicals. To investigate the effects of food and gender on the bioavailability of BB, GA, GB and GC after oral administration of GTL extract, a rapid UPLC-MS/MS method was developed and validated. A reversed phase C18 column (100mm×2.1mm, i.d., 1.7µm) and a mobile phase consisted of methanol and 1mM ammonium acetate (70/30, v/v) were employed. Compared with the fasted group, the t1/2 values for BB, GA, GB and GC in fed were all increased (p<0.05), AUC0-t and AUC0-∞ values of BB, GA, GB and GC were all significantly increased (p<0.05), but the Cmax values of BB, GA, GB and GC were significantly decreased (p<0.05). In comparison with the male group, all of the t1/2 values and AUC0-t values for BB, GA, GB and GC in female were higher (p<0.05), but no statistical difference in Tmax values for BB, GA, GB and GC between these two groups. Food and gender factor showed significant effects on the pharmacokinetics of BB, GA, GB, and GC. The results suggested that oral doses of GTL should be lowered for fasted and female subjects, compared with the fed and male subjects, respectively.
Assuntos
Interações Alimento-Droga , Ginkgo biloba , Ginkgolídeos/administração & dosagem , Ginkgolídeos/farmacocinética , Lactonas/administração & dosagem , Lactonas/farmacocinética , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacocinética , Administração Oral , Animais , Área Sob a Curva , Disponibilidade Biológica , Cromatografia de Fase Reversa/métodos , Estabilidade de Medicamentos , Jejum/sangue , Feminino , Ginkgo biloba/química , Ginkgolídeos/sangue , Ginkgolídeos/isolamento & purificação , Meia-Vida , Lactonas/sangue , Lactonas/isolamento & purificação , Masculino , Extratos Vegetais/sangue , Extratos Vegetais/isolamento & purificação , Período Pós-Prandial , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Fatores Sexuais , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em TandemRESUMO
Ginkgolide B (GB), the primary active component ofGinkgo bilobaextracts, may have antitumor properties. The objective of this study was to determine the effects and possible mechanisms of GB in ovarian cancer cells. In this study, human ovarian cancer cell lines (SKOV3 and CAOV3) were treated with different concentrations of GB alone or in combination with Cis-diaminodichloroplatinum (CDDP). An MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed to determine cell viability. The apoptosis rates of cells were measured by flow cytometric analysis. The expression of apoptosis-associated and proliferation-associated proteins was detected by Western blot. The cytotoxicity of GB was analyzed using a lactate dehydrogenase assay. Treatment with 100 µM GB for 3 days significantly inhibited SKOV3 and CAOV3 cell proliferation by 57.3% and 63.1% compared with control cells, respectively, as determined by MTT assay. Similarly, the apoptotic cell population was increased when treated with GB in a dose-dependent manner both in SKOV3 and CAOV3 cells. These effects were characterized by the upregulation of p21, p27, cleaved capase-3, and cleaved caspase-8 and downregulation of cyclin D1. In addition, a combined treatment of low concentrations of GB and CDDP showed an additive effect on the inhibition of SKOV3 cell proliferation. Furthermore, GB had significantly less cytotoxicity than CDDP in normal human ovarian surface epithelial cells. This study suggests that GB can be proposed as an effective antiproliferative and apoptosis-inducing agent with interesting translational application in ovarian cancers, used in addition to conventional chemotherapy.
Assuntos
Antineoplásicos/farmacologia , Cisplatino/farmacologia , Ginkgolídeos/farmacologia , Lactonas/farmacologia , Neoplasias Ovarianas/tratamento farmacológico , Antineoplásicos/administração & dosagem , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cisplatino/administração & dosagem , Relação Dose-Resposta a Droga , Feminino , Citometria de Fluxo , Ginkgo biloba/química , Ginkgolídeos/administração & dosagem , Ginkgolídeos/isolamento & purificação , Humanos , Lactonas/administração & dosagem , Lactonas/isolamento & purificação , Neoplasias Ovarianas/patologiaRESUMO
Terpene lactones such as bilobalide, ginkgolides A, B, C, and J are major bioactive compounds of Ginkgo biloba L. Purification of these compounds is tedious due to their similar chemical properties. For the purpose of developing an effective and efficient method for both analytical and preparative separation of terpene lactones in G. biloba, an innovative orthogonality-enhanced high-speed countercurrent chromatography (HSCCC) method was established. Taking advantage of quantitative (1)H NMR (qHNMR) methodology, partition coefficients (K) of individual terpene lactones were calculated directly from crude G. biloba leaf extract, using their H-12 signals as distinguishing feature. The partitioning experiment assisted the design of a two dimensional (2D) HSCCC procedure using a pair of orthogonal HSCCC solvent systems (SSs), ChMWat +4 and HEMSoWat +3/0.05%. It was surprising that the resolution of ginkgolides A and B was improved by 25% in the HEMWat +3 SS modified with 0.5% DMSO. Consequently, all five terpene lactones could be well separated with qHNMR purity>95% from G. biloba leaf extract. The separation was further evaluated by offline qHNMR analysis of HSCCC fractions associated with Gaussian curve fitting. The results showed less than 2% error in HSCCC retention predicted from the partitioning experiment. This compelling consistency demonstrates that qHNMR-derived K determination ("K-by-NMR") can be used to predict CCC fractionation and target purification of analytes from complex mixtures. Furthermore, Gaussian curve fitting enabled an accurate prediction of less than 2% impurity in the CCC fraction, which demonstrates its potential as a powerful tool to study the presence of minor constituents, especially when they are beyond the detection limit of conventional spectroscopic detectors.
Assuntos
Distribuição Contracorrente/métodos , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Lactonas/isolamento & purificação , Ressonância Magnética Nuclear Biomolecular/métodos , Ginkgolídeos/química , Ligação de Hidrogênio , Lactonas/química , Extratos Vegetais/química , Folhas de Planta/químicaRESUMO
To screen the presence of ginkgolide B-producing endophytic fungi from the root bark of Ginkgo biloba, a total of 27 fungal isolates, belonging to 6 different genus, were isolated from the internal root bark of the plant Ginkgo biloba. The fungal isolates were fermented on solid media and their metabolites were analyzed by TLC. The obtained potential ginkgolides-producing fungus, the isolate SYP0056 which was identified as Fusarium oxysporum, was successively cultured in the liquid fermentation media, and its metabolite was analyzed by HPLC. The ginkgolide B was successfully isolated from the metabolite and identified by HPLC/ESI-MS and (13)C-NMR. The current research provides a new method to produce ginkgolide B by fungal fermentation, which could overcome the natural resource limitation of isolating from the leaves and barks of the plant Ginkgo biloba.
Assuntos
Produtos Biológicos/química , Fusarium/metabolismo , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Lactonas/isolamento & purificação , Endófitos/metabolismo , Fermentação , Ginkgo biloba/microbiologia , Ginkgolídeos/metabolismo , Lactonas/metabolismo , Casca de Planta , Raízes de PlantasRESUMO
Two new diterpenoid compounds, ginkgolide P(1) and ginkgolide Q(2), were isolated from the leaves of Ginkgo biloba L. Their structures were elucidated by various spectroscopic methods, and the structure of 1 was further confirmed by X-ray crystallographic analysis. The activities of the compounds were evaluated against platelet aggregation induced by platelet activating factor (PAF), and the preliminary structure-activity relationship was also discussed.
Assuntos
Diterpenos/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Animais , China , Cristalografia por Raios X , Diterpenos/química , Diterpenos/farmacologia , Medicamentos de Ervas Chinesas/química , Ginkgolídeos/química , Ginkgolídeos/farmacologia , Lactonas/química , Lactonas/isolamento & purificação , Lactonas/farmacologia , Medicina Tradicional Chinesa , Folhas de Planta/química , Plantas Medicinais/química , Fator de Ativação de Plaquetas/efeitos dos fármacos , Agregação Plaquetária/efeitos dos fármacos , Coelhos , Relação Estrutura-AtividadeRESUMO
In this study, with flavonol glycosides (FG) and terpene lactones (TL) in ginkgo biloba extract (GBE) as the targets for separation, we investigated the effectiveness of molecular docking in adsorbent screening. Several polyamine-modified methyl acylate-co-divinylbenzene (MA-co-DVB) adsorbent models were built, and their affinity to rutin, quercetin and ginkgolide B (GB) was evaluated via molecular docking. The model of ethylenediamine-modified adsorbent showed the largest difference in affinity between to GB and to quercetin as well as rutin, and thus this adsorbent could have the best separation performance. The results of the subsequently conducted static adsorption and dynamic adsorption experiments correlated well with docking results. Finally, using ethylenediamine-modified MA-co-DVB adsorbent, nearly complete separation of the FG and TL in GBE was simply achieved by one step of adsorption-desorption. Thus, the reported molecular docking method is expected to be helpful for rapid adsorbent screening.
Assuntos
Ginkgo biloba/química , Modelos Químicos , Extratos Vegetais/química , Adsorção , Cromatografia Líquida de Alta Pressão , Estabilidade de Medicamentos , Ginkgolídeos/química , Ginkgolídeos/isolamento & purificação , Interações Hidrofóbicas e Hidrofílicas , Lactonas/química , Lactonas/isolamento & purificação , Modelos Moleculares , Extratos Vegetais/isolamento & purificação , Quercetina/química , Quercetina/isolamento & purificação , Reprodutibilidade dos Testes , Rutina/química , Rutina/isolamento & purificação , TermodinâmicaRESUMO
OBJECTIVE: To establish a simple extraction, isolation and purification method for ginkgolide B from ginkgo leaf. METHOD: The optimum conditions of extraction, isolation and purification were studied by taking the transfer rate of ginkgolide B as index. RESULT: Ginkgo leaf was extracted with 70% ethanol for three times, the extracts were concentrated to remove ethanol and diluted by water till the crude drug density reached 0.1 g x mL(-1). The dilution was adsorbed with HPD-450 macroporous resin. The impurities were eluted with 20% ethanol and ginkgolide B was eluted with 80% ethanol. Then the 80% ethanol eluant was concentrated and crystallized. Finally the crude crystals were recrystallized with isopropanol. The purity of the ginkgolide B recrystallization was 95%. CONCLUSION: The process was stable and easy to operate, which was suited to industrialized production.
Assuntos
Fracionamento Químico/métodos , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Lactonas/isolamento & purificação , Medicamentos de Ervas Chinesas/análise , Ginkgolídeos/análise , Lactonas/análise , Folhas de Planta/químicaRESUMO
OBJECTIVE: To establish the technology for extraction of ginkgolides from Ginkgo Biloba with alcohol-water. METHOD: The parameters such as alcohol concentration, pH of extracting solution, ratio of dosage liquor, temperature and time, the extraction of ginkgolides from G. biloba was investigated, and its parameters were optimized. RESULT: The optimized parameters were alcohol concentration 30%, extracting temperature 50 degrees C, extracting time 2 h, pH 5 solid-liquid ratio 1:15. CONCLUSION: This method has the merits of low cost and simple operation.
Assuntos
Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Ginkgolídeos/análise , Extratos Vegetais/análise , TemperaturaRESUMO
Eleven new metabolites, butanolides 1-6, lignan derivatives 7-9, sesquiterpene 10, and 3',4'-seco-flavane derivative 11, have been isolated from an ethanol extract of Machilus wangchiana. Twenty known compounds, including ginkgolides A and B (16 and 17), were also isolated. Their structures and absolute configurations were determined by spectroscopic and chemical methods. Compounds 7, 8a, 8b, 9, 11, (+)-guaiacin (12), meso-dihydroguaiaretic acid (13), and hamabiwalactone A (15) showed potent in vitro activities against the release of beta-glucuronidase in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor (PAF), with 42.5-75.6% inhibition at 10(-5) M. Compounds 8, 8a, 8b, 9, and 11 reduced dl-galactosamine (GalN)-induced hepatocyte (WB-F344 cells) damage with 39.4 +/- 6.3% to 53.6 +/- 3.5% inhibition at 10(-4) M. Isomahubannolide-23 (14) was cytotoxic against human stomach cancer (BGC-823) and ovarian cancer (A2780) cell lines, with IC(50) values of 0.13 and 2.66 muM, respectively.
Assuntos
4-Butirolactona/análogos & derivados , 4-Butirolactona/isolamento & purificação , Antineoplásicos Fitogênicos/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Flavonoides/isolamento & purificação , Lauraceae/química , Lignanas/isolamento & purificação , Sesquiterpenos/isolamento & purificação , 4-Butirolactona/química , 4-Butirolactona/farmacologia , Animais , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Flavonoides/química , Flavonoides/farmacologia , Ginkgolídeos/isolamento & purificação , Glucuronidase/sangue , Glucuronidase/efeitos dos fármacos , Humanos , Lactonas/isolamento & purificação , Lignanas/química , Lignanas/farmacologia , Estrutura Molecular , Neutrófilos/efeitos dos fármacos , Casca de Planta/química , Ratos , Sesquiterpenos/química , Sesquiterpenos/farmacologia , EstereoisomerismoRESUMO
A single dose by gavage of bilobalide (30 mg/kg) was found to produce a time-dependent induction of hepatic cytochrome P450 (CYP) enzyme activity and protein expression in rats. An RT-PCR study further showed that mRNA expression of CYP2B was maximal at 6 h. Plasma and liver bilobalide concentration in rats following administration of Ginkgo biloba extract equivalent to bilobalide of approximately 40 mg/kg showed a similar response to that exhibited by mRNA expression. These findings suggest that bilobalide markedly induced hepatic CYPs, but the induction could be mitigated due to rapid elimination from the liver.
Assuntos
Ciclopentanos/farmacologia , Sistema Enzimático do Citocromo P-450/efeitos dos fármacos , Furanos/farmacologia , Ginkgolídeos/farmacologia , Animais , Ciclopentanos/isolamento & purificação , Ciclopentanos/farmacocinética , Sistema Enzimático do Citocromo P-450/metabolismo , Indução Enzimática/efeitos dos fármacos , Furanos/isolamento & purificação , Furanos/farmacocinética , Regulação da Expressão Gênica/efeitos dos fármacos , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Ginkgolídeos/farmacocinética , Fígado/enzimologia , Fígado/metabolismo , Masculino , Extratos Vegetais/farmacocinética , Extratos Vegetais/farmacologia , RNA Mensageiro/efeitos dos fármacos , RNA Mensageiro/metabolismo , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de TempoRESUMO
Ginkgo biloba is one of the most popular herbal medicines in the world, due to its purported pharmacological effects, including memory-enhancing, cognition-improving, and antiplatelet effects. The study aimed to investigate the activity and expression of cytochrome P450 (CYP) 3A in human and rat primary hepatocytes treated with standardized G. biloba extract (100, 500, and 2500 ng/ml) for 72 hr, and to measure the protein expression of CYP3A in human and rat primary hepatocytes treated with bilobalide (2, 10, and 50 ng/ml) and ginkgolides B (2, 10, and 50 ng/ml). The activity of CYP3A was measured by the quantification of dehydronifedipine formation using a validated tandem liquid chromatography mass spectrometry (LC/MS/MS) method. The levels of mRNA and protein of CYP3A were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blotting analysis, respectively. The G. biloba extract at 100-2,500 ng/ml significantly induced the activity, protein and mRNA expression of CYP3A in a dose-dependent manner in human and rat primary hepatocytes. Bilobalide at 2-50 ng/ml significantly increased CYP3A protein expression in a dose-dependent manner in human and rat primary hepatocytes. However, ginkgolide B did not affect CYP3A protein expression in vitro. The results indicate that G. biloba extract pretreatment significantly induced the expression of CYP3A protein and mRNA and increased CYP3A activity, and there was no significant species difference between human and rat. G. biloba may cause potential interactions with substrate drugs of CYP3A. Bilobalide might play a key role in the enzyme-inducing effects of G. biloba extract. Further study is needed to identify the substances in GBE that induce CYPs in vivo, and elucidate the molecular mechanism of CYP3A induction by GBE and bilobalides.
Assuntos
Citocromo P-450 CYP3A/efeitos dos fármacos , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Ginkgo biloba/química , Extratos Vegetais/farmacologia , Adulto , Idoso , Animais , Ciclopentanos/administração & dosagem , Ciclopentanos/isolamento & purificação , Ciclopentanos/farmacologia , Citocromo P-450 CYP3A/genética , Relação Dose-Resposta a Droga , Indução Enzimática/efeitos dos fármacos , Feminino , Furanos/administração & dosagem , Furanos/isolamento & purificação , Furanos/farmacologia , Ginkgolídeos/administração & dosagem , Ginkgolídeos/isolamento & purificação , Ginkgolídeos/farmacologia , Hepatócitos/efeitos dos fármacos , Hepatócitos/enzimologia , Humanos , Lactonas/administração & dosagem , Lactonas/isolamento & purificação , Lactonas/farmacologia , Masculino , Pessoa de Meia-Idade , Nifedipino/análogos & derivados , Nifedipino/metabolismo , Extratos Vegetais/administração & dosagem , RNA Mensageiro/efeitos dos fármacos , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-DawleyRESUMO
A method for identification and quantitative determination of ginkgolides A, B, and C and bilobalide in liquid and dry extracts of Ginkgo biloba extracts has been developed. Determinations made by employing capillary gas chromatography technique with FID detection were preceded by derivatization using BSTFA with TMCS addition at 120 degrees C. Cholesterol was used as an internal standard. Validation of the method shows no interferences with concurrent constituents; average resolution (R), controlled for peaks of cholesterol and ginkgolide A was 1.53 (SD = 0.06). In the temperature program used (from 50 degrees C to 300 degrees C) the analyte retention times range from 11.2 min. (bilobalide) to 13.8 min. (ginkgolide C) and are of high repeatability of relative values (RRT): RSD = 0.05% / 0.07% for ginkgolides. High correlation coefficients (r), detector signal linearity: from 0.99962 for ginkgolide C to 0.99985 for ginkgolide A were obtained within the concentration range under investigation. The method is of high sensitivity: limits of detection and limits of determination are 35 pg and 44 pg for bilobalide, respectively, while for ginkgolides (Gk) are: 78 pg and 92 pg for GkA, 57 pg and 68 pg for GkB, and 213 pg and 320 pg for GkC.
Assuntos
Cromatografia Gasosa/métodos , Ginkgo biloba/química , Extratos Vegetais/química , Ciclopentanos/análise , Ciclopentanos/isolamento & purificação , Ionização de Chama , Furanos/análise , Furanos/isolamento & purificação , Ginkgolídeos/análise , Ginkgolídeos/isolamento & purificação , Lactonas/análise , Lactonas/isolamento & purificação , Folhas de Planta , Reprodutibilidade dos Testes , TemperaturaRESUMO
OBJECTIVE: To investigate the effects of ginkgolides injection on experimental cerebral ischemia and its related mechanism of action. METHOD: The middle cerebral artery occlusion (MACO) model was induced by the FeCl3-occluding method to explore the protective effects of ginkgolides injection on the score of neurological deficits, the rate of cerebral infarction and the histomorphology of cerbral ischemia in rats. Thrombosis formation in vivo was induced by adrenaline-collagen in mice to explore the antithrombotic effect. Platelet aggregation was induced by ADP and hemorrheological parameters with hyper-viscosity by dextran T-500 were used to explore the effects of antiplatelet aggregation and decreasing viscosity of blood. RESULT: Ginkgolides injection could markedly decrease the infarct size and behavior deficits score, inhibit the thrombus formation in mice, decrease blood viscosity and ameliorate hemorrheological parameters in rat. CONCLUSION: Ginkgolides injection has the protective effects on focal cerebral ischemia, and its mechanism may be relative to its inhibition of platelet-dependent thrombosis and amelioration of hemarheological partments.
Assuntos
Encéfalo/patologia , Ginkgo biloba , Ginkgolídeos/farmacologia , Infarto da Artéria Cerebral Média/fisiopatologia , Fármacos Neuroprotetores/farmacologia , Animais , Comportamento Animal/efeitos dos fármacos , Viscosidade Sanguínea/efeitos dos fármacos , Feminino , Ginkgo biloba/química , Ginkgolídeos/administração & dosagem , Ginkgolídeos/isolamento & purificação , Infarto da Artéria Cerebral Média/patologia , Injeções , Trombose Intracraniana/patologia , Masculino , Camundongos , Fármacos Neuroprotetores/administração & dosagem , Fármacos Neuroprotetores/isolamento & purificação , Agregação Plaquetária/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Sprague-DawleyRESUMO
AIM: Ginkgo biloba extract is a natural product used widely for cerebral and cardiovascular diseases. It is mainly composed of terpene lactones (ginkgolide A and B) and flavone glycosides (eg quercetin and kaempferol). To better understand the cardiac electromechanical action of Ginkgo biloba extract in normal and diabetic states, this study was designed to examine the effect of ginkgolide B on cardiomyocyte contractile function under normal and high-glucose environments. METHODS: Isolated adult rat ventricular myocytes were cultured for 6 h in a serum-free medium containing either normal (NG; 5.5 mmol/L) or high (HG; 25.5 mmol/L) glucose with or without ginkgolide B (0.5-2.0 microg/mL). Mechanical properties were evaluated using the IonOptix MyoCam system. Contractile properties analyzed included peak shortening (PS), maximal velocity of shortening/relengthening (+/-dl/dt), time-to-PS (TPS) and time-to-90% relengthening (TR90). Levels of essential Ca(2+) regulatory proteins sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA2a), phospholamban (PLB) and Na(+)-Ca(2+) exchanger (NCX) were assessed by Western blotting. RESULTS: Ginkgolide B nullified HG-induced prolongation in TR90. However, ginkgolide B depressed PS, +/-dl/dt and shortened TPS in NG and HG cells. Ginkgolide B also prolonged TR90 in NG cells. Western blot analysis revealed that HG upregulated SERCA2a and downregulated PLB expression without affecting that of NCX. Ginkgolide B disrupted the NG-HG response pattern in SERCA2a and NCX without affecting that of PLB. CONCLUSION: Ginkgolide B affects cardiomyocyte contractile function under NG or HG environments in a paradoxical manner, which may be attributed to uneven action on Ca(2+) regulatory proteins under NG and HG conditions.
Assuntos
Proteínas de Ligação ao Cálcio/metabolismo , Ginkgolídeos/farmacologia , Lactonas/farmacologia , Contração Miocárdica/efeitos dos fármacos , Miócitos Cardíacos , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo , Animais , Tamanho Celular/efeitos dos fármacos , Células Cultivadas , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Glucose/farmacologia , Ventrículos do Coração , Lactonas/isolamento & purificação , Masculino , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Plantas Medicinais/química , Ratos , Ratos Sprague-Dawley , Trocador de Sódio e Cálcio/metabolismoRESUMO
OBJECTIVE: To observe the effects of ginkgolides on gene expression of hepatic cytochrome P-450 in rats. METHOD: Sprague-Dawley rats were administered ginkgolides (100 mg x kg(-1) body weight) through oral gavage once daily for four consecutive days. The level of gene expression in liver tissues was analyzed by competitive reverse transcription-polymerase chain reaction (competitive RT-PCR). RESULT: A single and prospective band of CYP1A1, CYP1A2, CYP2B1/B2, CYP2C11, CYP2E1, CYP4A1 and cyclophilin was observed after polymerase chain reaction (PCR) when the reactive system of reverse transcription (RT) had no target RNA, which confirmed the competitor had a specific capacity to bind to the CYP or cyclophilin primer. CYP1A1 mRNA was not dectectable in the livers of untreated control rats and ginkgolides-treated rats. The levels of CYP2C11 and CYP2E1 were not changed by ginkgolides treatment. In contrast, the levels of gene expression for CYP1A2 and CYP2B1/B2 were decreased, however, the levels of gene expression for CYP3A1 and CYP4A1 in ginkgolides group were distinctly increased compared with the control. CONCLUSION: A specific effect of ginkgolides on cytochrome P-450 gene expression was observed in this investigation. Ginkgolides had various effects on different cytochrome P-450 isoforms.
Assuntos
Citocromo P-450 CYP1A1/biossíntese , Sistema Enzimático do Citocromo P-450/biossíntese , Ginkgolídeos/farmacologia , Fígado/metabolismo , Animais , Hidrocarboneto de Aril Hidroxilases/biossíntese , Hidrocarboneto de Aril Hidroxilases/genética , Citocromo P-450 CYP1A1/genética , Citocromo P-450 CYP1A2/biossíntese , Citocromo P-450 CYP1A2/genética , Citocromo P-450 CYP2B1/biossíntese , Citocromo P-450 CYP2B1/genética , Citocromo P-450 CYP3A , Sistema Enzimático do Citocromo P-450/genética , Família 4 do Citocromo P450 , Regulação da Expressão Gênica , Ginkgo biloba/química , Ginkgolídeos/isolamento & purificação , Masculino , Plantas Medicinais/química , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Distribuição Aleatória , Ratos , Ratos Sprague-DawleyRESUMO
Ginkgo biloba extract EGb 761 has been used for many years to treat age-related cognitive disorders including Alzheimer's disease. EGb 761 given shortly after initiating mitochondrial damage by sodium nitroprusside (nitric oxide donor) improved the mitochondrial membrane potential of PC12 cells significantly and dose dependently. Under these conditions, EGb 761 also reversed the decrease in ATP production. In addition, similar protection against oxidative damage was found in dissociated brain cells and isolated brain mitochondria after in vitro or in vivo treatment with EGb 761. Moreover, PC12 cells bearing an Alzheimer's disease-related mutation in the amyloid precursor protein, which leads to enhanced beta amyloid production, showed greater benefit from treatment with EGb 761 than did control cells. Taken together, our findings clearly show stabilization and protection of mitochondrial function as a specific and very sensitive property of EGb 761 at therapeutically relevant doses.