RESUMEN
The unicellular yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe are widely used eukaryotic model organisms. Research exploiting the tractability of these model systems has contributed significantly to our understanding of a wide range of fundamental processes. In this article, we outline the features of yeast that have similarly been exploited for undergraduate research training. We selected examples from published literature that demonstrate the utility of the yeast system for research-based learning embedded in the curriculum. We further describe a project which we designed for the team-based final-year dissertation projects module on our transnational joint programme, which investigates whether the expression and functions of the budding yeast RPL36 ribosomal protein paralogs are influenced by the overlapping long non-coding RNA genes. Students carry out the experimental procedures in a 2-week timetabled teaching block and exercise widely applicable biochemical techniques, including aseptic yeast cell culture and sample collection, RNA isolation, qRT-PCR quantitation, protein extraction and Western blot analysis, and cell cycle progression patterns using light microscopy and flow cytometry. It is challenging to design training programmes for undergraduates that are meaningful as well as practical and economical, but it is possible to transform active research projects into authentic research experiences. We consider yeast to be an ideal model organism for such projects. These can be adapted to the constraints of course schedules and explore fundamental biochemical topics which are evolutionarily conserved from yeast to mammals.
Asunto(s)
ARN Largo no Codificante , Saccharomyces cerevisiae , Animales , Humanos , Saccharomyces cerevisiae/genética , Proteínas Ribosómicas/genética , Estudiantes , Envejecimiento , MamíferosRESUMEN
Acquiring skills needed to plan and conduct research and communicate research outcomes are key learning aims in biochemical and biomedical disciplines. Final-year projects/dissertations are high-impact educational activities that commonly feature in undergraduate curricula. When cohort sizes exceed infrastructure and staff capacity, traditional models of supervised projects may not be feasible. This case study aims to share one model of practice with colleagues similarly engaged in design and delivery of final-year projects and research. Here, we outline the implementation and evaluation of a team-based, final-year research module on a transnational joint programme. Investigative Skills module was piloted in 2016-2017 and continues to run annually for >100 students. The research component is conducted over a timetabled, two-week block. In student questionnaires, the majority of respondents agreed that the projects were authentic, interesting and appropriate. The favourite aspect for most of the respondents was performing experimental work/doing research. Over 80% agreed that working in teams was conducive to accomplishing their goals, and their ideal team size is three to five students per team. The majority agreed that there was sufficient experimental work to do, but that more than two weeks practical time would be beneficial. The feedback has given insight into the whole of the student research experience of Investigative Skills, which is a sustainable model for authentic dissertation research for large cohorts.
Asunto(s)
Curriculum , Aprendizaje , HumanosRESUMEN
An important aspect of teaching and learning is the student's response to and enaction of formative feedback. Few studies have examined how students interpret the formative feedback provided by tutors and translate these into action. In order to understand this better, we carried out a retrospective analysis of student responses to iterative formative feedback. The context of the present study is an extracurricular project where undergraduate biomedical science students on a transnational education programme competed in an extracurricular, cross-disciplinary video competition. Based on detailed analysis of provided feedback and subsequent enaction of the feedback, we found that students were active participants in the feedback process, engaged with the meaningful feedback critically, and incorporated the relevant changes to improve the final output. In this article, we will give our perspectives on how students understand, process and act on feedback, and also examine the features of the feedback that elicited these responses from students.
Asunto(s)
Educación de Pregrado en Medicina , Estudiantes de Medicina , Educación de Pregrado en Medicina/métodos , Retroalimentación , Retroalimentación Formativa , Humanos , Estudios RetrospectivosRESUMEN
During meiosis, two rounds of chromosome segregation occur after a single round of DNA replication, producing haploid progeny from diploid progenitors. Three innovations in chromosome behaviour during meiosis I accomplish this unique division. First, crossovers between maternal and paternal sister chromatids (detected cytologically as chiasmata) bind replicated maternal and paternal chromosomes together. Second, sister kinetochores attach to microtubules from the same pole (mono-polar orientation), causing maternal and paternal centromere pairs (and not sister chromatids) to be separated. Third, sister chromatid cohesion near centromeres is preserved at anaphase I when cohesion along chromosome arms is destroyed. The finding that destruction of mitotic cohesion is regulated by Polo-like kinases prompted us to investigate the meiotic role of the yeast Polo-like kinase Cdc5. We show here that cells lacking Cdc5 synapse homologues and initiate recombination normally, but fail to efficiently resolve recombination intermediates as crossovers. They also fail to properly localize the Lrs4 (ref. 3) and Mam1 (ref. 4) monopolin proteins, resulting in bipolar orientation of sister kinetochores. Cdc5 is thus required both for the formation of chiasmata and for cosegregation of sister centromeres at meiosis I.
Asunto(s)
Proteínas de Ciclo Celular , Centrómero/genética , Segregación Cromosómica/genética , Células Eucariotas/metabolismo , Meiosis/genética , Proteínas Quinasas/genética , Proteínas de Saccharomyces cerevisiae , Levaduras/enzimología , Levaduras/genética , Transportadoras de Casetes de Unión a ATP/genética , Transportadoras de Casetes de Unión a ATP/metabolismo , Células Cultivadas , Cromosomas/genética , Replicación del ADN/genética , Técnica del Anticuerpo Fluorescente , Regulación Fúngica de la Expresión Génica/genética , Cinetocoros/metabolismo , Proteínas Quinasas/deficiencia , Proteínas Serina-Treonina Quinasas , Proteínas de Schizosaccharomyces pombe/genética , Proteínas de Schizosaccharomyces pombe/metabolismoRESUMEN
Meiosis, the developmental programme generating haploid gametes from diploid precursors, requires two cell divisions and many innovations. In budding yeast, a large number of genes are expressed exclusively during meiosis while others are repressed compared to vegetative growth. Microarray analysis has shown that gene expression during meiosis is highly regulated, and has been used to classify yeast genes according to meiotic temporal expression pattern. In this study, we have begun to investigate the kinetics of meiotic protein expression using a proteomics approach. 2-D DIGE was used to characterise the temporal protein expression patterns of the budding yeast pH 4-7 proteome in meiosis. More than 1400 meiotic protein spots were visualised and at least 63 spots were temporally regulated during meiosis in a statistically significant manner. Gel spots with significant expression changes were excised and 26 unique proteins were identified using LC-MS/MS. The identified proteins could be classified into functional categories and the genes encoding a number of these were previously shown to be involved in yeast sporulation and meiosis. This data set was used to assemble the first differential 2-D PAGE map of budding yeast meiosis, which can be accessed through a web server. This work represents one of the first quantitative proteomic analyses of meiosis in yeast and will provide a valuable resource for future investigations.
Asunto(s)
Meiosis/genética , Proteoma/análisis , Proteínas de Saccharomyces cerevisiae/análisis , Proteínas de Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/genética , Concentración de Iones de HidrógenoRESUMEN
Meiosis is the cell division that generates haploid gametes from diploid precursors. To provide insight into the functional proteome of budding yeast during meiosis, a 2-D DIGE kinetic approach was used to study proteins in the pH 6-11 range. Nearly 600 protein spots were visualised and 79 spots exhibited statistically significant changes in abundance as cells progressed through meiosis. Expression changes of up to 41-fold were detected and protein sequence information was obtained for 48 spots. Single protein identifications were obtained for 21 spots including different gel mobility forms of 5 proteins. A large number of post-translational events are suggested for these proteins, including processing, modification and import. The data are incorporated into an online 2-DE map of meiotic proteins in budding yeast, which extends our initial DIGE investigation of proteins in the pH 4-7 range. Together, the analyses provide peptide sequence data for 84 protein spots, including 50 single-protein identifications and gel mobility isoforms of 8 proteins. The largest classes of identified proteins include carbon metabolism, protein catabolism, protein folding, protein synthesis and the oxidative stress response. A number of the corresponding genes are required for yeast meiosis and recent studies have identified similar classes of proteins expressed during mammalian meiosis. This proteomic investigation and the resulting protein reference map make an important contribution towards a more detailed molecular view of yeast meiosis.
Asunto(s)
Proteoma/química , Proteínas de Saccharomyces cerevisiae/química , Bases de Datos de Proteínas , Concentración de Iones de Hidrógeno , Meiosis , Proteoma/clasificación , Proteínas de Saccharomyces cerevisiae/clasificación , Electroforesis Bidimensional Diferencial en GelRESUMEN
Sample degradation is a common problem in all types of proteomic analyses as it generates protein and peptide fragments that can interfere with analytical results. An important step in preventing such artefacts is to preserve the native, intact proteome as early as possible during sample preparation prior to proteomic analysis. Using the budding yeast Saccharomyces cerevisiae, we have evaluated the effects of trichloroacetic acid (TCA) and thermal treatments prior to protein extraction as a means to minimise proteolysis. TCA precipitation is commonly used to inactivate proteases; thermal stabilisation is used to heat samples to approximately 95 degrees C to inactivate enzyme activity. The efficacy of these methods was also compared with that of protease inhibitors and lyophilisation. Sample integrity was assessed by 2-D PAGE and a selection of spots was identified by MS/MS. The analysis showed that TCA or thermal treatment significantly reduced the degree of degradation and that these pre-treatment protocols were more effective than treatment with either protease inhibitors or lyophilisation. This study establishes standardised sample preparation methods for the reproducible analysis of protein patterns by 2-D PAGE in yeast, and may also be applicable to other proteomic analyses such as gel-free-based quantitation methods.
Asunto(s)
Preservación Biológica/métodos , Proteoma/análisis , Proteínas de Saccharomyces cerevisiae/análisis , Saccharomyces cerevisiae/química , Electroforesis en Gel Bidimensional , Estabilidad Proteica , Espectrometría de Masas en Tándem , Temperatura , Ácido Tricloroacético/químicaRESUMEN
In meiosis, accumulation of recombination intermediates or defects in chromosome synapsis trigger checkpoint-mediated arrest in prophase I. Such 'checkpoints' are important surveillance mechanisms that ensure temporal dependence of cell cycle events. The budding yeast Polo-like kinase, Cdc5, has been identified as a key regulator of the meiosis I chromosome segregation pattern. Here we have analysed the role of Cdc5 in the recombination checkpoint and observed that Polo-like kinase is not required for checkpoint activation in yeast meiosis. Surprisingly, depletion of CDC5 in the Drad17 checkpoint-defective background resulted in nuclear fragmentation to levels even higher than that observed in Ddmc1 Drad17 cells that bypass the checkpoint arrest despite accumulating DNA double-strand breaks. The spindle morphology of Cdc5-depleted cells included short, thick metaphase I spindles in mononucleate cells and disassembled spindles in binucleate and tetranucleate cells, although this phenotype does not appear to be the cause of the nuclear fragmentation. An exaggeration of chromosome synapsis defects occurred in Cdc5-depleted Drad17 cells and may contribute to the nuclear fragmentation phenotype. The analysis also uncovered a role for Cdc5 in maintaining spindle integrity in Ddmc1 Drad17 cells. Further analysis confirmed that adaptation to DNA damage does occur in meiosis and that CDC5 is required for this process. The cdc5-ad mutation that renders cells unable to adapt to DNA damage in mitosis did not affect checkpoint adaptation in meiosis, indicating that the mechanisms of checkpoint adaptation in mitosis and meiosis are not fully conserved.