Comparative analysis of microbial communities from different full-scale haloalkaline biodesulfurization systems.

In biodesulfurization (BD) at haloalkaline and dO2-limited conditions, sulfide-oxidizing bacteria (SOB) effectively convert sulfide into elemental sulfur that can be used in agriculture as a fertilizer and fungicide. Here we show which bacteria are present in this biotechnological process. 16S rRNA gene amplicon sequencing of biomass from ten reactors sampled in 2018 indicated the presence of 444 bacterial Amplicon Sequence Variants (ASVs). A core microbiome represented by 30 ASVs was found in all ten reactors, with Thioalkalivibrio sulfidiphilus as the most dominant species. The majority of these ASVs are phylogenetically related to bacteria previously identified in haloalkaline BD processes and in natural haloalkaline ecosystems. The source and composition of the feed gas had a great impact on the microbial community composition followed by alkalinity, sulfate, and thiosulfate concentrations. The halophilic SOB of the genus Guyparkeria (formerly known as Halothiobacillus) and heterotrophic SOB of the genus Halomonas were identified as potential indicator organisms of sulfate and thiosulfate accumulation in the BD process. KEY POINTS: • Biodesulfurization (BD) reactors share a core microbiome • The source and composition of the feed gas affects the microbial composition in the BD reactors • Guyparkeria and Halomonas indicate high concentrations of sulfate and thiosulfate in the BD process.

Continuous electron shuttling by sulfide oxidizing bacteria as a novel strategy to produce electric current.

Sulfide oxidizing bacteria (SOB) are widely applied in industry to convert toxic H2S into elemental sulfur. Haloalkaliphilic planktonic SOB can remove sulfide from solution under anaerobic conditions (SOB are 'charged'), and release electrons at an electrode (discharge of SOB). The effect of this electron shuttling on product formation and biomass growth is not known. Here, we study and demonstrate a continuous process in which SOB remove sulfide from solution in an anaerobic 'uptake chamber', and shuttle these electrons to the anode of an electrochemical cell, in the absence of dissolved sulfide. Two experiments over 31 and 41 days were performed. At a sulfide loading rate of 1.1 mmolS/day, electricity was produced continuously (3 A/m2) without dissolved sulfide in the anolyte. The main end product was sulfate (56% in experiment 1% and 78% in experiment 2), and 87% and 77% of the electrons in sulfide were recovered as electricity. It was found that the current density was dependent on the sulfide loading rate and not on the anode potential. Biological growth occurred, mainly at the anode as biofilm, in which the deltaproteobacterial genus Desulfurivibrio was dominating. Our results demonstrate a novel strategy to produce electricity from sulfide in an electrochemical system.

Biologically enhanced hydrogen sulfide absorption from sour gas under haloalkaline conditions.

We studied a biotechnological desulfurization process for removal of toxic hydrogen sulfide (H2S) from sour gas. The process consists of two steps: i) Selective absorption of H2S into a (bi)carbonate solution in the absorber column and ii) conversion of sulfide to sulfur by sulfide oxidizing bacteria (SOB) in the aerated bioreactor. In previous studies, several physico-chemical factors were assessed to explain the observed enhancement of H2S absorption in the absorber, but a full explanation was not provided. We investigated the relation between the metabolic activity of SOB and the enhancement factor. Two continuous experiments on pilot-scale were performed to determine H2S absorption efficiencies at different temperatures and biomass concentrations. The absorption efficiency improved at increasing temperatures, i.e. H2S concentration in the treated gas decreased from 715 ±â€¯265 ppmv at 25.4 °C to 69 ±â€¯25 ppmv at 39.4 °C. The opposite trend is expected when H2S absorption is solely determined by physico-chemical factors. Furthermore, increasing biomass concentrations to the absorber also resulted in decreased H2S concentrations in the treated gas, from approximately 6000 ppmv without biomass to 1664 ±â€¯126 ppmv at 44 mg N/L. From our studies it can be concluded that SOB activity enhances H2S absorption and leads to increased H2S removal efficiencies in biotechnological gas desulfurization.

Treatment of real flue gas desulfurization wastewater in an autotrophic biocathode in view of elemental sulfur recovery: Microbial communities involved.

Sulfur oxide emissions can lead to acidic precipitation and health concerns. Flue gas desulfurization (FGD) systems treat these emissions generating a wastewater with high-sulfate content. This work is the first attempt to treat this effluent with bioelectrochemical systems (BES) in order to recover elemental sulfur, a technology that allows the treatment of several wastewaters that lack of electron donor. The sulfate treatment and elemental sulfur recovery have been studied in a biocathode with simultaneous sulfate reduction to sulfide and partial sulfide oxidation, comparing the performance obtained with synthetic and real wastewater. A decrease of the sulfate removal rate (SRR) from 108 to 73mgS-SO42-L-1d-1 was observed coupled to an increase in the elemental sulfur recovery from 1.4 to 27mgS-S0L-1d-1. This elemental sulfur recovered as a solid from the real wastewater represented a 64% of the theoretical elemental sulfur produced (the elemental sulfur corresponded to a 72% of the solid weight). In addition, microbial communities analysis of the membrane and cathode biofilms and planktonic biomass showed that the real wastewater allowed a higher growth of sulfur oxidizing bacteria (SOB) adapted to more complex waters as Halothiobacillus sp. while decreasing the relative abundance of sulfate reducing bacteria (SRB).

Elucidating functional microorganisms and metabolic mechanisms in a novel engineered ecosystem integrating C, N, P and S biotransformation by metagenomics.

Denitrifying sulfur conversion-associated enhanced biological phosphorous removal (DS-EBPR) system is not only a novel wastewater treatment process, but also an ideal model for microbial ecology in a community context. However, it exists the knowledge gap on the roles and interactions of functional microorganisms in the DS-EBPR system for carbon (C), nitrogen (N), phosphorus (P) and sulfur (S) bioconversions. We use genome-resolved metagenomics to build up an ecological model of microbial communities in a lab-scale DS-EBPR system with stable operation for more than 400 days. Our results yield 11 near-complete draft genomes that represent a substantial portion of the microbial community (39.4%). Sulfate-reducing bacteria (SRB) and sulfide-oxidizing bacteria (SOB) promote complex metabolic processes and interactions for C, N, P and S conversions. Bins 1-4 and 10 are considered as new potential polyphosphate-accumulating organisms (PAOs), in which Bins 1-4 can be considered as S-related PAOs (S-PAOs) with no previously cultivated or reported members. Our findings give an insight into a new ecological system with C, N, P and S simultaneous bioconversions and improve the understanding of interactions among SRB, SOB, denitrifiers and PAOs within a community context.

Evaluation of key parameters on simultaneous sulfate reduction and sulfide oxidation in an autotrophic biocathode.

Bioelectrochemical systems (BESs) are being studied as an alternative technology for the treatment of several kinds of wastewaters with a lack of electron donor such as high-strength sulfate wastewaters. This study evaluates different parameters that influence the simultaneous sulfate reduction and sulfide oxidation in an autotrophic biocathode: ion-exchange membrane (IEM), cathodic pH and cathode potential. Two different membranes were studied to evaluate sulfate and sulfide adsorption and diffusion from the cathode to the anode, observing that a cation-exchange membrane (CEM) widely decreased these effects. Three different cathode pH (5.5, 7 and 8.5) were studied in a long-term operation observing that pH = 7 was the optimal for sulfate removal, achieving reduction rates around 150 mg S-SO42- L-1 d-1. Microbial community analysis of the cathode biofilm demonstrated a high abundance of sulfate-reducing bacteria (SRB, 67% at pH 7, 60% at pH 8.5 and 42% at pH 5.5), mainly Desulfovibrio sp. at pH 5.5 and 7 and Desulfonatronum sp. at pH 8.5. The cathode potential also was studied from -0.7 to -1.2 V vs. SHE achieving sulfate removal rates higher than 700 mg S-SO42- L-1 d-1 at cathode potentials from -1.0 to -1.2 V vs. SHE. Also, the highest cathodic recovery and the highest sulfur species imbalance were observed at a cathode potential of -1.0 V vs. SHE, which indicated a higher elemental sulfur production.

Treatment of high-strength sulfate wastewater using an autotrophic biocathode in view of elemental sulfur recovery.

Treatment of high-strength sulfate wastewaters is becoming a research issue not only for its optimal management but also for the possibility of recovering elemental sulfur. Moreover, sulfate-rich wastewater production is expected to grow due to the increased SO2 emission contained in flue gases which are treated by chemical absorption in water. Bioelectrochemical systems (BESs) are a promising alternative for sulfate reduction with a lack of electron donor, since hydrogen can be generated in situ from electricity. However, complete sulfate reduction leads to hydrogen sulfide as final sulfur compound. This work is the first to demonstrate that, in addition to an efficient sulfate-rich wastewater treatment, elemental sulfur could be recovered in a biocathode of a BES under oxygen limiting conditions. The key of the process is the biological oxidation of sulfide to elemental sulfur simultaneously to the sulfate reduction in the cathode using the oxygen produced in the anode that diffuses through the membrane. High sulfate reduction rates (up to 388 mg S-SO42- L-1 d-1) were observed linked to a low production of sulfide. Accumulation of elemental sulfur over graphite fibers of the biocathode was demonstrated by energy dispersive spectrometry, discarding the presence of metal sulfides. Microbial community analysis of the cathode biofilm demonstrated the presence of sulfate-reducing bacteria (mainly Desulfovibrio sp.) and sulfide-oxidizing bacteria (mainly Sulfuricurvum sp.). Hence, this biocathode allows simultaneous biological sulfate reduction and biological sulfide oxidation to elemental sulfur, opening up a novel process for recovering sulfur from sulfate-rich wastewaters.