RESUMEN
ABSTRACT Objective: To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material and Methods: The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 7:30 and 9:00 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4°C, and the supernatants (500µl) were stored at −80°C until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively. Results: Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring. Conclusion: The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls.
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Humanos , Masculino , Femenino , Saliva , Espectroscopía de Resonancia Magnética/instrumentación , Mucopolisacaridosis/patología , Metabolómica , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Estudios Transversales/métodosRESUMEN
MRI at ultra-high field (UHF, ≥7 T) provides a natural strategy for improving the quality of X-nucleus magnetic resonance spectroscopy and imaging due to the intrinsic benefit of increased signal-to-noise ratio. Considering that RF coils require both local transmission and reception at UHF, the designs of double-tuned coils, which often consist of several layers of transmit and receive resonant elements, become quite complex. A few years ago, a new type of RF coil, ie a dipole antenna, was developed and used for human body and head imaging at UHF. Due to the mechanical and electrical simplicity of dipole antennas, combining an X-nucleus surface loop array with 1 H dipoles can substantially simplify the design of a double-tuned UHF human head array coil. Recently, we developed a novel bent folded-end dipole transceiver array for human head imaging at 9.4 T. The new eight-element dipole array demonstrated full brain coverage, and transmit efficiency comparable to that of the substantially more complex 16-element surface loop array. In this work, we developed, constructed and evaluated a double-tuned 13 C/1 H human head 9.4 T array consisting of eight 13 C transceiver surface loops and eight 1 H transceiver bent folded-end dipole antennas all placed in a single layer. We showed that interaction between loops and dipoles can be minimized by placing four 1 H traps into each 13 C loop. The presented double-tuned RF array coil substantially simplifies the design as compared with the common double-tuned surface loop arrays. At the same time, the coil demonstrated an improved 1 H longitudinal coverage and good transmit efficiency.
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Espectroscopía de Resonancia Magnética con Carbono-13/instrumentación , Cabeza/diagnóstico por imagen , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Simulación por Computador , Campos Electromagnéticos , Humanos , Fantasmas de ImagenRESUMEN
Tuberculosis (TB) is a major cause of morbidity and mortality in children, and early diagnosis and treatment are crucial to reduce long-term morbidity and mortality. In this study, we explore whether urine nuclear magnetic resonance (NMR)-based metabolomics could be used to identify differences in the metabolic response of children with different diagnostic certainty of TB. We included 62 children with signs and symptoms of TB and 55 apparently healthy children. Six of the children with presumptive TB had bacteriologically confirmed TB, 52 children with unconfirmed TB, and 4 children with unlikely TB. Urine metabolic fingerprints were identified using high- and low-field proton NMR platforms and assessed with pattern recognition techniques such as principal components analysis and partial least squares discriminant analysis. We observed differences in the metabolic fingerprint of children with bacteriologically confirmed and unconfirmed TB compared to children with unlikely TB (p = 0.041 and p = 0.013, respectively). Moreover, children with unconfirmed TB with X-rays compatible with TB showed differences in the metabolic fingerprint compared to children with non-pathological X-rays (p = 0.009). Differences in the metabolic fingerprint in children with different diagnostic certainty of TB could contribute to a more accurate characterisation of TB in the paediatric population. The use of metabolomics could be useful to improve the prediction of TB progression and diagnosis in children.
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Metaboloma , Metabolómica/métodos , Espectroscopía de Protones por Resonancia Magnética/métodos , Tuberculosis Pulmonar/diagnóstico , Tuberculosis Pulmonar/orina , Estudios de Casos y Controles , Niño , Preescolar , Análisis Discriminante , Diagnóstico Precoz , Femenino , Humanos , Lactante , Análisis de los Mínimos Cuadrados , Masculino , Metabolómica/estadística & datos numéricos , Mycobacterium tuberculosis/crecimiento & desarrollo , Mycobacterium tuberculosis/patogenicidad , Análisis de Componente Principal , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Tuberculosis Pulmonar/microbiología , Tuberculosis Pulmonar/patologíaRESUMEN
The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.
Asunto(s)
Geobacillus/metabolismo , Virus de la Influenza A/metabolismo , Proteínas de la Membrana/química , Neisseria gonorrhoeae/metabolismo , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Proteínas de la Membrana Bacteriana Externa/química , Humanos , Campos Magnéticos , Modelos Moleculares , Proteínas Quinasas/química , Estructura Secundaria de Proteína , Proteínas de la Matriz Viral/química , Canales Aniónicos Dependientes del Voltaje/químicaRESUMEN
Nicotinamide adenine dinucleotide (NAD) is an important cofactor of energy-producing pathways. The redox ratio (NAD+/NADH) reflects the cellular oxidoreductive state. Oxidative stress and redox dysregulation have been suggested to contribute to various neurological diseases. The assessment of NAD content has been recently demonstrated in large animals and human brains by 31P magnetic resonance spectroscopy. However, its measurement in small rodents has never been attempted. The purpose of this study was to investigate, in vivo, the NAD content during mouse brain neurodevelopment. 31P-MR-spectra were acquired in the mouse brain at postnatal days P20, P40, P90 and P250 at 14.1 T using a 3D-localization sequence. High spectral quality was achieved at 14.1 T. NAD+ and NADH were quantified with mean Cramér-Rao lower bound of 10% and 14%, respectively. An increase in NAD+/NADH was observed from P20 to P250 due to a decrease in [NADH]. The intracellular pH was significantly reduced with age, while the free [Mg2+] in the brain was significantly increased. This study demonstrates for the first time the feasibility of the measurement of NAD content in vivo in mouse brains during development, which opens the prospect of longitudinally studying energy metabolism and redox dysfunction in mouse models of brain pathology.
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Encéfalo/metabolismo , Metabolismo Energético , NAD/metabolismo , Espectroscopía de Protones por Resonancia Magnética/métodos , Animales , Encéfalo/crecimiento & desarrollo , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Oxidación-Reducción , Estrés Oxidativo , Fantasmas de Imagen , Espectroscopía de Protones por Resonancia Magnética/instrumentaciónRESUMEN
Betamethasone is a glucocorticoid authorised in cattle for the treatment of metabolic and inflammatory diseases, but, in Europe, it is illegally employed to improve productive performances. LC-MS/MS is the official control method of veterinary drugs residues in food of animal origin. An experimental study was developed to evaluate the feasibility of proton magnetic resonance spectroscopy (1H-MRS) as a potential alternative approach to detect the presence of betamethasone residues. Eight rat liver samples were collected 24 h post-betamethasone-treatment from experimental and control animals and were analysed by 1H-MRS using a 7-Tesla MRI scanner. 1H-MR reference spectra both of the Bentelan formulation used for treatment, and of three solutions of betamethasone in dimethyl sulphoxide (DMSO) at 5, 10 and 100 mM, respectively, were acquired to fit analyte-peaks in the liver samples spectra. Betamethasone-peaks were found only in the 100 mM betamethasone in DMSO solution spectrum. Betamethasone residues were not detected in any of the tissue samples analysed, probably related to the low concentration of injected drug. These findings allow us to establish, for the first time in the literature, the detection limit (in the range 10-100 mM) of betamethasone for the 7-Tesla MRI scanner used here. Given this very-low sensitivity, we conclude that the evaluated 1H-MR spectroscopy approach is not suitable for the detection of betamethasone residues in edible tissues, since the maximum residue limit imposed by Commission Regulation (EC) 37/2010 for betamethasone in the liver, and metabolic concentrations required to be detected in animal samples from livestock, are far below the detection limit we found.
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Betametasona/análisis , Residuos de Medicamentos/análisis , Hígado/química , Animales , Masculino , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Ratas , Ratas WistarRESUMEN
NMR applied to living organisms is arguably the ultimate tool for understanding environmental stress responses and can provide desperately needed information on toxic mechanisms, synergistic effects, sublethal impacts, recovery, and biotransformation of xenobiotics. To perform in vivo NMR spectroscopy, a flow cell system is required to deliver oxygen and food to the organisms while maintaining optimal line shape for NMR spectroscopy. In this tutorial, two such flow cell systems and their constructions are discussed: (a) a single pump high-volume flow cell design is simple to build and ideal for organisms that do not require feeding (i.e., eggs) and (b) a more advanced low-volume double pump flow cell design that permits feeding, maintains optimal water height for water suppression, improves locking and shimming, and uses only a small recirculating volume, thus reducing the amount of xenobiotic required for testing. In addition, key experimental aspects including isotopic enrichment, water suppression, and 2D experiments for both 13 C enriched and natural abundance organisms are discussed.
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Espectroscopía de Resonancia Magnética con Carbono-13/instrumentación , Espectroscopía de Resonancia Magnética con Carbono-13/métodos , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Espectroscopía de Protones por Resonancia Magnética/métodos , Animales , Chlamydomonas reinhardtii/química , Daphnia/químicaRESUMEN
Importance: The use of high-field magnetic resonance spectroscopy (MRS) in multiple brain regions of a large population of human participants facilitates in vivo study of localized or diffusely altered brain metabolites in patients with first-episode psychosis (FEP) compared to healthy participants. Objective: To compare metabolite levels in 5 brain regions between patients with FEP (evaluated within 2 years of onset) and healthy controls, and to explore possible associations between targeted metabolite levels and neuropsychological test performance. Design, Setting, and Participants: Cross-sectional design used 7-T MRS at a research MR imaging facility in participants recruited from clinics at the Johns Hopkins Schizophrenia Center and the local population. Eighty-one patients who had received a DSM-IV diagnosis of FEP within the last 2 years and 91 healthy age-matched (but not sex-matched) volunteers participated. Main Outcomes and Measures: Brain metabolite levels including glutamate, glutamine, γ-aminobutyric acid (GABA), N-acetylaspartate, N-acetylaspartyl glutamate, and glutathione, as well as performance on neuropsychological tests. Results: The mean (SD) age of 81 patients with FEP was 22.3 (4.4) years and 57 were male, while the mean (SD) age of 91 healthy participants was 23.3 (3.9) years and 42 were male. Compared with healthy participants, patients with FEP had lower levels of glutamate (F1,162 = 8.63, P = .02), N-acetylaspartate (F1,161 = 5.93, P = .03), GABA (F1,163 = 6.38, P = .03), and glutathione (F1,162 = 4.79, P = .04) in the anterior cingulate (all P values are corrected for multiple comparisons); lower levels of N-acetylaspartate in the orbitofrontal region (F1,136 = 7.23, P = .05) and thalamus (F1,133 = 6.78, P = .03); and lower levels of glutathione in the thalamus (F1,135 = 7.57, P = .03). Among patients with FEP, N-acetylaspartate levels in the centrum semiovale white matter were significantly correlated with performance on neuropsychological tests, including processing speed (r = 0.48; P < .001), visual (r = 0.33; P = .04) and working (r = 0.38; P = .01) memory, and overall cognitive performance (r = 0.38; P = .01). Conclusions and Relevance: Seven-tesla MRS offers insights into biochemical changes associated with FEP and may be a useful tool for probing brain metabolism that ranges from neurotransmission to stress-associated pathways in participants with psychosis.
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Encéfalo/metabolismo , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Trastornos Psicóticos/metabolismo , Ácido Aspártico/análogos & derivados , Ácido Aspártico/metabolismo , Estudios de Casos y Controles , Estudios Transversales , Dipéptidos/metabolismo , Femenino , Ácido Glutámico/metabolismo , Glutamina/metabolismo , Humanos , Masculino , Pruebas Neuropsicológicas , Adulto Joven , Ácido gamma-Aminobutírico/metabolismoRESUMEN
Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins. These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site. Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
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Anticoagulantes/análisis , Contaminación de Medicamentos/prevención & control , Heparina/análisis , Espectroscopía de Protones por Resonancia Magnética/métodos , Animales , Anticoagulantes/química , Sitios de Unión , Bovinos , Minería de Datos , Heparina/química , Mucosa Intestinal/química , Oligosacáridos/química , Análisis de Componente Principal , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Control de Calidad , Ovinos , Programas Informáticos , PorcinosRESUMEN
Cardiac hypertrophy (CH) is a major risk factor for many serious heart diseases. Sustained CH is catastrophic, resulting in cardiac dysfunction that eventually leads to heart failure (HF). Baoyuan decoction (BYD) is a famous traditional Chinese medicine (TCM) formula for supplementing and reinforcing Qi, clinically used for the treatment of cardiovascular diseases (CVDs). However, the therapeutic effects of BYD on CH remain unidentified. We herein investigated the effect of BYD on isoproterenol (ISO)-induced CH in rats and the underlying mechanisms by comprehensive pharmacodynamics and 1H NMR-based dynamic metabolomics analysis of the plasma and urine samples. Results showed that BYD treatment markedly attenuated ISO-induced CH as evidenced by decreasing the left ventricular wall thickness, pathological cardiomyocyte hypertrophy, myocardial collagen fiber deposition and apoptosis, and plasma natriuretic peptide levels. Multivariate trajectory analysis revealed that the BYD treatment could restore the CH-disturbed plasma and urinary metabolite profiles towards the normal metabolic status featuring with a time-dependent tendency. Moreover, the key metabolic alterations in CH rats at different BYD-treated time stages involved energy metabolism, oxidative stress responses, amino acid metabolism, and gut microbiota metabolism. Of particularly, the significant roles of BYD for treating CH lie in the improvement of cardiac energy generation and antioxidant capacity. Our investigation provides a holistic view of BYD for therapeutic intervention of CH through monitoring of the dynamic metabolic changes and the results indicate that BYD may be applied as a potential agent for treating CH.
Asunto(s)
Cardiomegalia/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Medicina Tradicional China/métodos , Metabolómica/métodos , Animales , Cardiomegalia/inducido químicamente , Cardiomegalia/metabolismo , Cardiomegalia/patología , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos/uso terapéutico , Humanos , Isoproterenol/toxicidad , Masculino , Metabolómica/instrumentación , Miocardio/metabolismo , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/metabolismo , Estrés Oxidativo/efectos de los fármacos , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Espectroscopía de Protones por Resonancia Magnética/métodos , Ratas , Ratas Sprague-DawleyRESUMEN
PURPOSE: To perform in vitro high-resolution 900 MHz magnetic resonance spectroscopy (NMR) analysis of human brain tumor tissue extracts and analyze for the oncometabolite 2-hydroxyglutarate (2HG) and other brain metabolites, not only for 1H but also for 13C with indirect detection by heteronuclear single quantum correlation (HSQC). MATERIAL AND METHODS: Four surgically removed human brain tumor tissue samples were used for extraction and preparation of NMR samples. These tissue samples were extracted with 4% perchloric acid and chloroform, freeze-dried, then dissolved into 0.28 mL of deuterium oxide (D2O, 99.9 atom % deuterium) containing 0.025 wt % sodium 3-(trimethylsilyl)propionate-2,2,3,3-d4 (TSP). All samples were adjusted to pH range of 6.9-7.1 before finally transferred to 5 mm Shigemi™ NMR microtube. NMR experiments were performed on Bruker DRX 900 MHz spectrometer with 1H/13C/15N Cryo-probe™ with Z-gradient, without further temperature control for the samples. All chemical shift values were presented relative to TSP at 0.00 ppm for both 1H and 13C. 1H 1D, 1H-13C HSQC, 1H-1H correlation spectroscopy (COSY) and 1H-13C heteronuclear multiple bond correlation (HMBC) spectra were acquired and analyzed. RESULTS: 2-hydroxyglutarate, an oncometabolite associated with gliomas with IDH mutations, was successfully detected and assigned by both 1H-13C HSQC and 1H-1H COSY experiments as well as 1H 1D experiments in two of the tissue samples. In particular, to our knowledge this work shows the first example of detecting 900 MHz 13C-NMR spectral lines of 2-hydroxyglutarate in human brain tumor tissue samples. In addition to the oncometabolite 2-hydroxyglutarate, at least 42 more metabolites were identified from our series of NMR experiment. CONCLUSION: The detection of 2-hydroxyglutarate and other metabolites can be facilitated by homonuclear and heteronuclear two-dimensional 900 MHz NMR spectroscopy even in case of real tumor tissue sample extracts without physical separation of metabolites.
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Neoplasias Encefálicas/metabolismo , Espectroscopía de Resonancia Magnética con Carbono-13/métodos , Glioma/metabolismo , Glutaratos/análisis , Espectroscopía de Protones por Resonancia Magnética/métodos , Extractos de Tejidos/análisis , Encéfalo/metabolismo , Encéfalo/patología , Espectroscopía de Resonancia Magnética con Carbono-13/instrumentación , Cloroformo/química , Óxido de Deuterio/química , Humanos , Concentración de Iones de Hidrógeno , Percloratos/química , Propionatos/química , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Reproducibilidad de los Resultados , Compuestos de Trimetilsililo/químicaRESUMEN
The present study sought to identify the key biomarkers and pathways involved in the induction of allergic sensitization to ovalbumin and to elucidate the potential anti-anaphylaxis property of Clinacanthus nutans (Burm. f.) Lindau water leaf extract, a Southeast Asia herb in an in vivo ovalbumin-induced active systemic anaphylaxis model evaluated by 1H-NMR metabolomics. The results revealed that carbohydrate metabolism (glucose, myo-inositol, galactarate) and lipid metabolism (glycerol, choline, sn-glycero-3-phosphocholine) are the key requisites for the induction of anaphylaxis reaction. Sensitized rats treated with 2000â¯mg/kg bw C. nutans extract before ovalbumin challenge showed a positive correlation with the normal group and was negatively related to the induced group. Further 1H-NMR analysis in complement with Kyoto Encyclopedia of Genes and Genomes (KEGG) reveals the protective effect of C. nutans extract against ovalbumin-induced anaphylaxis through the down-regulation of lipid metabolism (choline, sn-glycero-3-phosphocholine), carbohydrate and signal transduction system (glucose, myo-inositol, galactarate) and up-regulation of citrate cycle intermediates (citrate, 2-oxoglutarate, succinate), propanoate metabolism (1,2-propanediol), amino acid metabolism (betaine, N,N-dimethylglycine, methylguanidine, valine) and nucleotide metabolism (malonate, allantoin). In summary, this study reports for the first time, C. nutans water extract is a potential anti-anaphylactic agent and 1H-NMR metabolomics is a great alternative analytical tool to explicate the mechanism of action of anaphylaxis.
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Acanthaceae/química , Anafilaxia/prevención & control , Extractos Vegetales/farmacología , Sustancias Protectoras/farmacología , Espectroscopía de Protones por Resonancia Magnética/métodos , Anafilaxia/sangre , Anafilaxia/inmunología , Anafilaxia/orina , Animales , Biomarcadores/análisis , Metabolismo de los Hidratos de Carbono/efectos de los fármacos , Metabolismo de los Hidratos de Carbono/inmunología , Carbohidratos/sangre , Carbohidratos/orina , Modelos Animales de Enfermedad , Humanos , Metabolismo de los Lípidos/efectos de los fármacos , Metabolismo de los Lípidos/inmunología , Lípidos/sangre , Lípidos/orina , Masculino , Metabolómica/instrumentación , Metabolómica/métodos , Ovalbúmina/inmunología , Extractos Vegetales/uso terapéutico , Hojas de la Planta/química , Sustancias Protectoras/uso terapéutico , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Ratas , Ratas Sprague-DawleyRESUMEN
Metabolic reprogramming has been associated to a plethora of diseases, and there has been increased demand for methodologies able to determine the metabolic alterations that characterize the pathological states and help developing metabolically centered therapies. In this chapter, methodologies for monitoring TCA cycle turnover and its interaction with pyruvate cycling and anaplerotic reactions will be presented. These methodologies are based in the application of stable 13C isotope "tracers"/substrates and 13C-NMR isotopomer analysis of metabolic intermediates. These methodologies can be applied at several organizational levels, ranging from isolated organelles and organs to whole organisms/humans. For the sake of simplicity, only very simple and well-defined models will be presented, including isolated heart mitochondria and isolated perfused hearts and livers.
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Isótopos de Carbono/análisis , Espectroscopía de Resonancia Magnética con Carbono-13/métodos , Metabolismo Energético , Mitocondrias Cardíacas/metabolismo , Perfusión/métodos , Animales , Espectroscopía de Resonancia Magnética con Carbono-13/instrumentación , Corazón , Isomerismo , Hígado , Ratones , Perfusión/instrumentación , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Espectroscopía de Protones por Resonancia Magnética/métodos , RatasRESUMEN
Huntington's disease (HD) is a genetic neurodegenerative disorder caused by an abnormal expansion of a CAG repeat located in the gene encoding for huntingtin protein. This mutation induces the expression of a polyglutamine stretch in the mutated protein resulting in the modification of various biological properties of the wild-type protein and the progressive appearance of motor, cognitive, and psychiatric disorders that are typically associated to this condition. Although the exact neuropathological mechanisms of degeneration are still not fully understood, HD pathology is characterized by severe neuronal losses in various brain regions including the basal ganglia and many cortical areas. Early signs of astrogliosis may precede actual neuronal degeneration. Early metabolic impairment at least in part associated with mitochondrial complex II deficiency may play a key role in huntingtin-induced mechanisms of neurodegeneration. Clinical trials are actively prepared including various gene-silencing approaches aiming at decreasing mutated huntingtin production. However, with the lack of a specific imaging biomarker capable of visualizing mutated huntingtin or huntingtin aggregates, there is a need for surrogate markers of huntingtin neurodegeneration. MRI and caudate nucleus atrophy is one of the most sensitive imaging biomarkers of HD. As such it can be used as a means to study disease progression and potential halting of the neurodegenerative process by therapeutic intervention, but this marker relies on actual neuronal loss which is a somewhat a late event in the pathology. As a means to develop, characterize and evaluate new, potentially earlier biomarkers of HD pathology we have recently embarked on a series of NMR developments looking for brain imaging techniques that allow for noninvasive longitudinal evaluation/characterization of functional alterations in animal models of HD. This chapter describes an assemblage of innovative NMR methods that have proved useful in detecting pathological cell dysfunctions in various preclinical models of HD.
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Encéfalo/diagnóstico por imagen , Modelos Animales de Enfermedad , Neuroimagen Funcional/métodos , Enfermedad de Huntington/diagnóstico por imagen , Microscopía Intravital/métodos , Animales , Encéfalo/metabolismo , Encéfalo/patología , Medios de Contraste/administración & dosificación , Neuroimagen Funcional/instrumentación , Ácido Glutámico/metabolismo , Humanos , Proteína Huntingtina/genética , Proteína Huntingtina/metabolismo , Enfermedad de Huntington/genética , Enfermedad de Huntington/patología , Microscopía Intravital/instrumentación , Imagen por Resonancia Magnética/instrumentación , Imagen por Resonancia Magnética/métodos , Ratones , Ratones Transgénicos , Imagen Molecular/instrumentación , Imagen Molecular/métodos , Primates , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Espectroscopía de Protones por Resonancia Magnética/métodosRESUMEN
Phenethylamines (PEAs) are popular substances found in weight-loss and sports nutrition supplements. They are generally pharmacologically active and primarily affect the sympathetic nervous system. Many PEAs are synthetic chemicals and are on the prohibited list of the World Anti-Doping Agency. In this study, nuclear magnetic resonance (NMR) spectroscopy was applied to detect and identify the presence of PEAs in sports dietary supplements without the need for chromatographic separation or pre-knowledge on formulation. Eight PEAs, viz. phenethylamine, synephrine, oxilofrine, hordenine, ß-methylphenethylamine, N-methyltyramine, octopamine and deterenol, were identified from 32 dietary supplements sold in the US market. Furthermore, a quantitative NMR method was developed and validated for simultaneous determination of the concentrations of the PEAs. The study demonstrated that NMR could be a potential tool to monitor and detect PEAs or other ingredients in dietary supplements.
Asunto(s)
Diosgenina/análisis , Doping en los Deportes/prevención & control , Sustancias para Mejorar el Rendimiento/análisis , Fenetilaminas/análisis , Fitosteroles/análisis , Espectroscopía de Protones por Resonancia Magnética/métodos , Deuterio/química , Diosgenina/química , Humanos , Sustancias para Mejorar el Rendimiento/química , Fenetilaminas/química , Fitosteroles/química , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Coloración y Etiquetado/métodosRESUMEN
Identification of NMR-based metabolic indexes is limited by the deleterious effects of copious proteins and lipoproteins in the serum that accentuate the need for advance and high-throughput method. We tried to explore the use of a novel filtration (2KDa molecular weight cut-off) approach to remove the proteins from serum following use of less sample volume (only 150⯵L of filtered serum), combining an array of 1D/2D NMR experiments (at 800â¯MHz spectrometer), spiking experiments with standard compounds, and validated by mass spectrometry. This novel method enabled the identification of a large number (nâ¯=â¯73) of metabolites and their percentage of abundance in the present study cohort. Mass spectrometry further validates and confirms the presence of all these 73 metabolites using same filtered serum. This study reveals seven new metabolites (citrulline, inosine, taurine, trimethyl amine, methylmalonate, uracil, methanol) in filtered serum using 1D/2D NMR spectroscopy that were not observed in earlier available literature using protein precipitation approach. This novel method delineates volatile metabolites, nitrogenous bases and nucleosides that may provide a milestone for the identification of inborn error of metabolism, pathogenicity at molecular level, disease identification and prognosis, and forensic studies using minimal volume of filtered serum samples and NMR spectroscopy.
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Espectrometría de Masas/métodos , Metaboloma , Metabolómica/métodos , Espectroscopía de Protones por Resonancia Magnética/métodos , Suero/química , Estudios de Factibilidad , Ciencias Forenses/instrumentación , Ciencias Forenses/métodos , Humanos , Límite de Detección , Espectrometría de Masas/instrumentación , Errores Innatos del Metabolismo/sangre , Errores Innatos del Metabolismo/diagnóstico , Errores Innatos del Metabolismo/metabolismo , Metabolómica/instrumentación , Filtros Microporos , Peso Molecular , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Suero/metabolismo , Compuestos Orgánicos Volátiles/análisis , Compuestos Orgánicos Volátiles/químicaRESUMEN
Magnetic Resonance Spectroscopy (MRS) allows for a non-invasive and non-ionizing determination of in vivo tissue concentrations and metabolic turn-over rates of more than 20 metabolites and compounds in the central nervous system of humans. The aim of this review is to give a comprehensive overview about the advantages, challenges and advances of ultra-high field MRS with regard to methodological development, discoveries and applications from its beginnings around 15 years ago up to the current state. The review is limited to human brain and spinal cord application at field strength of 7T and 9.4T and includes all relevant nuclei (1H, 31P, 13C).
Asunto(s)
Encéfalo/diagnóstico por imagen , Encéfalo/metabolismo , Neuroimagen/métodos , Resonancia Magnética Nuclear Biomolecular/métodos , Espectroscopía de Protones por Resonancia Magnética/métodos , Humanos , Neuroimagen/instrumentación , Resonancia Magnética Nuclear Biomolecular/instrumentación , Espectroscopía de Protones por Resonancia Magnética/instrumentaciónRESUMEN
Magnetic resonance spectroscopic imaging (MRSI) is a promising technique for mapping the spatial distribution of multiple metabolites in the human brain. These metabolite maps can be used as a diagnostic tool to gain insight into several biochemical processes and diseases in the brain. In comparison to lower field strengths, MRSI at ultra-high field strengths benefits from a higher signal to noise ratio (SNR) as well as higher chemical shift dispersion, and hence spectral resolution. This study combines the benefits of an ultra-high field magnet with the advantages of an ultra-short TE and TR single-slice FID-MRSI sequence (such as negligible J-evolution and loss of SNR due to T2 relaxation effects) and presents the first metabolite maps acquired at 9.4T in the healthy human brain at both high (voxel size of 97.6µL) and ultra-high (voxel size of 24.4µL) spatial resolutions in a scan time of 11 and 46min respectively. In comparison to lower field strengths, more anatomically-detailed maps with higher SNR from a larger number of metabolites are shown. A total of 12 metabolites including glutamate (Glu), glutamine (Gln), N-acetyl-aspartyl-glutamate (NAAG), Gamma-aminobutyric acid (GABA) and glutathione (GSH) are reliably mapped. Comprehensive description of the methodology behind these maps is provided.
Asunto(s)
Encéfalo/diagnóstico por imagen , Encéfalo/metabolismo , Procesamiento de Imagen Asistido por Computador/métodos , Neuroimagen/métodos , Espectroscopía de Protones por Resonancia Magnética/métodos , Adulto , Humanos , Espectroscopía de Protones por Resonancia Magnética/instrumentaciónRESUMEN
High resolution (600MHz) nuclear magnetic resonance (NMR) spectroscopy is used to distinguish heparin and low-molecular weight heparins (LMWHs) produced from porcine, bovine and ovine mucosal tissues as well as their blends. For multivariate analysis several statistical methods such as principal component analysis (PCA), factor discriminant analysis (FDA), partial least squares - discriminant analysis (PLS-DA), linear discriminant analysis (LDA) were utilized for the modeling of NMR data of more than 100 authentic samples. Heparin and LMWH samples from the independent test set (n=15) were 100% correctly classified according to its animal origin. Moreover, by using 1H NMR coupled with chemometrics and several batches of bovine heparins from two producers were differentiated. Thus, NMR spectroscopy combined with chemometrics is an efficient tool for simultaneous identification of animal origin and process based manufacturing difference in heparin products.
Asunto(s)
Anticoagulantes/análisis , Heparina de Bajo-Peso-Molecular/análisis , Heparina/análisis , Modelos Químicos , Tecnología Farmacéutica/métodos , Animales , Biotecnología/normas , Bovinos , Análisis Discriminante , Análisis de los Mínimos Cuadrados , Análisis Multivariante , Análisis de Componente Principal , Espectroscopía de Protones por Resonancia Magnética/instrumentación , Espectroscopía de Protones por Resonancia Magnética/métodos , Control de Calidad , Ovinos , Porcinos , Tecnología Farmacéutica/instrumentación , Tecnología Farmacéutica/normasRESUMEN
PURPOSE: To assess the clinical value of proton (1 H) MR spectroscopy in cervical carcinomas, in the prediction of poor prognostic human papillomavirus (HPV) genotypes as well as persistent disease following concurrent chemoradiotherapy (CCRT). MATERIALS AND METHODS: 1 H MR spectroscopy using external phase array coil was performed in 52 consecutive cervical cancer patients at 3 Tesla (T). Poor prognostic HPV genotypes (alpha-7 species or absence of HPV infection) and persistent cervical carcinoma after CCRT were recorded. Statistical significance was calculated with the Mann-Whitney two-sided nonparametric test and areas under the receiver operating characteristics curve (AUC) analysis. RESULTS: A 4.3-fold (P = 0.032) increased level of methyl resonance at 0.9 ppm was found in the poor prognostic HPV genotypes, mainly attributed to the presence of HPV18, with a sensitivity of 75%, a specificity of 81%, and an AUC of 0.76. Poor prognostic HPV genotypes were more frequently observed in patients with adeno-/adenosquamous carcinoma (Chi-square, P < 0.0001). In prediction of the four patients with persistent disease after CCRT, elevated methyl resonance demonstrated a sensitivity of 100%, a specificity of 74%, and an AUC of 0.82. CONCLUSION: 1 H MR spectroscopy at 3T can be used to depict the elevated lipid resonance levels in cervical carcinomas, as well as help to predict the poor prognostic HPV genotypes and persistent disease following CCRT. Further large studies with longer follow up times are warranted to validate our initial findings. LEVEL OF EVIDENCE: 1 J. Magn. Reson. Imaging 2017;45:899-907.