ABSTRACT
Profilin-1 (Pfn-1) is a ubiquitously expressed actin-binding protein that is essential for normal cell proliferation and migration. In breast cancer and several other adenocarcinomas, Pfn-1 expression is downregulated when compared to normal tissues. Previous studies from our laboratory have shown that genetically modulating Pfn-1 expression significantly impacts proliferation, migration, and invasion of breast cancer cells in vitro, and mammary tumor growth, dissemination, and metastatic colonization in vivo. Therefore, small molecules that can modulate Pfn-1 expression could have therapeutic potential in the treatment of metastatic breast cancer. The overall goal of this study was to perform a multiplexed phenotypic screen to identify compounds that inhibit cell motility through upregulation of Pfn-1. Screening of a test cassette of 1280 compounds with known biological activities on an Oris™ Pro 384 cell migration platform identified several agents that increased Pfn-1 expression greater than two-fold over vehicle controls and exerted anti-migratory effects in the absence of overt cytotoxicity in MDA-MB-231 human breast cancer cells. Concentration-response confirmation and orthogonal follow-up assays identified two bona fide inducers of Pfn-1, purvalanol and tyrphostin A9, that confirmed in single-cell motility assays and Western blot analyses. SiRNA-mediated knockdown of Pfn-1 abrogated the inhibitory effect of tyrphostin A9 on cell migration, suggesting Pfn-1 is mechanistically linked to tyrphostin A9's anti-migratory activity. The data illustrate the utility of the high-content cell motility assay to discover novel targeted anti-migratory agents by integrating functional phenotypic analyses with target-specific readouts in a single assay platform.
Subject(s)
Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Cell Movement , High-Throughput Screening Assays/methods , Profilins/metabolism , Adenine/pharmacology , Cell Line, Tumor , Cell Migration Assays , Cell Movement/drug effects , Female , Gene Knockdown Techniques , Humans , Reproducibility of Results , Small Molecule Libraries , Tyrphostins/pharmacologyABSTRACT
An assessment of the total protein composition of filovirus (ebolavirus and marburgvirus) virions is currently lacking. In this study, liquid chromatography-linked tandem mass spectrometry of purified ebola and marburg virions was performed to identify associated cellular proteins. Host proteins involved in cell adhesion, cytoskeleton, cell signaling, intracellular trafficking, membrane organization, and chaperones were identified. Significant overlap exists between this data set and proteomic studies of disparate viruses, including HIV-1 and influenza A, generated in multiple cell types. However, the great majority of proteins identified here have not been previously described to be incorporated within filovirus particles. Host proteins identified by liquid chromatography-linked tandem mass spectrometry could lack biological relevance because they represent protein contaminants in the virus preparation, or because they are incorporated within virions by chance. These issues were addressed using siRNA library-mediated gene knockdown (targeting each identified virion-associated host protein), followed by filovirus infection. Knockdown of several host proteins (e.g. HSPA5 and RPL18) significantly interfered with ebolavirus and marburgvirus infection, suggesting specific and relevant virion incorporation. Notably, select siRNAs inhibited ebolavirus, but enhanced marburgvirus infection, suggesting important differences between the two viruses. The proteomic analysis presented here contributes to a greater understanding of filovirus biology and potentially identifies host factors that can be targeted for antiviral drug development.
Subject(s)
Filoviridae/metabolism , Proteomics , RNA Interference , Viral Proteins/metabolism , Virion/metabolism , Amino Acid Sequence , Chromatography, Liquid , Electrophoresis, Polyacrylamide Gel , Gene Knockdown Techniques , Microscopy, Fluorescence , Molecular Sequence Data , Polymerase Chain Reaction , Tandem Mass Spectrometry , Viral Proteins/chemistry , Viral Proteins/geneticsABSTRACT
The voltage-gated potassium channel, Kv1.3, is specifically expressed on human lymphocytes, where it controls membrane potential and calcium influx. Blockade of Kv1.3 channels by margatoxin was previously shown to prevent T cell activation and attenuate immune responses in vivo. In the present study, a triterpene natural product, correolide, was found to block Kv1.3 channels in human and miniswine T cells by electrophysiological characterization. T cell activation events, such as anti-CD3-induced calcium elevation, IL-2 production, and proliferation were inhibited by correolide in a dose-dependent manner. More potent analogs were evaluated for pharmacokinetic profiles and subsequently tested in a delayed-type hypersensitivity (DTH) response to tuberculin in the miniswine. Two compounds were dosed orally, iv, or im, and both compounds suppressed DTH responses, demonstrating that small molecule blockers of Kv1.3 channels can act as immunosuppressive agents in vivo. These studies establish correolide and its derivatives as novel immunosuppressants.
Subject(s)
Immunosuppressive Agents/pharmacology , Potassium Channel Blockers , Potassium Channels, Voltage-Gated , Potassium Channels , T-Lymphocytes/drug effects , Triterpenes/pharmacology , Animals , Humans , Immunosuppressive Agents/chemistry , Ion Channel Gating , Kv1.3 Potassium Channel , Molecular Structure , Swine , Swine, Miniature , T-Lymphocytes/immunology , Triterpenes/chemistryABSTRACT
A new quantitative cytometric technique, termed the ArrayScanTM, is described and used to measure NF-kappaB nuclear translocation induced by interleukin (IL)-1 and tumor necrosis factor-alpha (TNFalpha). The amount of p65 staining is measured in both the nuclei defined by Hoechst 33342 labeling and in the surrounding cytoplasmic area within a preselected number of cells/well in 96-well plates. Using this technique in synchronously activated human chondrocytes or HeLa cells, NF-kappaB was found to move to the nucleus with a half-time of 7-8 min for HeLa and 12-13 min for chondrocytes, a rate in each case about 4-5 min slower than that of Ikappa Balpha degradation. IL-1 receptor antagonist and anti-TypeI IL-1 receptor antiserum on the one hand and anti-TNFalpha and monoclonal anti-TNF receptor 1 antibodies on the other hand could be shown to respectively inhibit IL-1 and TNFalpha stimulation in both cell types. In contrast, a polyclonal anti-TNF receptor 1 antiserum exhibited both a 50% agonism and a 50% antagonism to a TNFalpha stimulation in a dose-dependent fashion, indicating that subtle functional responses to complex agonist and antagonist stimuli could be measured. The effects of different proteasome inhibitors to prevent Ikappa Balpha degradation and subsequent NF-kappaB translocation could also be discriminated; Leu-Leu-Leu aldehyde was only a partial inhibitor with an IC50 of 2 microM, while clastolactacystin beta-lactone was a complete inhibitor with an IC50 of 10 microM. The nonselective kinase inhibitor K252a completely inhibited both IL-1 and TNFalpha stimulation in both cell types with an IC50 of 0.4 microM. This concentration, determined after a 20-min stimulation, was shown to be comparable with that obtained for inhibition of IL-6 production induced by a 100-fold lower IL-1 and TNFalpha concentration measured after 17 h of stimulation. These results suggest that the ArrayScanTM technology provides a rapid, sensitive, quantitative technique for measuring early events in the signal transduction of NF-kappaB.
Subject(s)
Cell Nucleus/metabolism , Interleukin-1/pharmacology , NF-kappa B/metabolism , Tumor Necrosis Factor-alpha/pharmacology , Biological Transport , Cell Compartmentation , Dose-Response Relationship, Drug , Fluorescent Antibody Technique , HeLa Cells , Humans , KineticsABSTRACT
Utilizing flow cytometry, we previously demonstrated that the potassium channel blocker margatoxin (MgTX) inhibits the [Ca2+]i transient involved in T-cell activation. We wished to extend these studies to single-cell transients using florescence digital-imaging microscopy (DIM). However, the most currently available temperature-regulation chambers reuse part or all of the apparatus and introduce compounds via perfusion. Thus, these apparatuses are not suitable for studies involving compounds that are particularly sticky. We have designed a dual-temperature regulation system that will maintain Nunc, eight-well, coverglass-bottom, disposable chambers, and three disposable addition pipets at 37 degrees C for physiological studies on an inverted digital-imaging microscope. We have demonstrated that calcium transients of human T lymphocytes can be initiated and monitored reproducibly during the addition of three distinct chemical species. The DIM results correlate with flow cytometry measurements in the number of responding cells and the heterogeneity of the response in both control and MgTX-inhibited cultures. Additionally, DIM revealed that the [Ca2+]i transient is more rapid than the flow-cytometric measurement indicated. The correlation between flow cytometry and DIM permits the amalgamation of these results in the interpretation of studies on the regulation of T-cell activation.
Subject(s)
Calcium/metabolism , Flow Cytometry , Image Processing, Computer-Assisted/instrumentation , Microscopy, Fluorescence/instrumentation , T-Lymphocytes/metabolism , Humans , In Vitro Techniques , Intracellular Fluid/drug effects , Intracellular Fluid/metabolism , Kinetics , Lymphocyte Activation , Neurotoxins/pharmacology , Potassium Channel Blockers , Scorpion Venoms , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , TemperatureABSTRACT
Assessment of DNA content by flow cytometry has largely depended on staining techniques which do not permit exclusion of dead cells from the data set. During studies of B cell activation in vitro, the large number of nonviable cells greatly affects the cell cycle distribution and thus the accurate evaluation of proliferation flow cytometry. This report describes the development of two dual staining techniques which use Hoechst 33342 and ethidium bromide excited by a single UV source to eliminate dead cells from the DNA histogram of the viable cells in murine B cell cultures. Hoechst 33342 and 0.62 micrograms/ml of ethidium bromide permit the evaluation of cell cycle distributions on the viable cells with a ratio gate. The combination of Hoechst 33342 and 6.2 micrograms/ml ethidium bromide results in the resolution of the two populations due to fluorescence energy transfer with a single PMT. Using this technique we demonstrated the simultaneous determination of DNA and RNA content on viable cells using only two PMTs. Both these techniques can be performed on either a laser or an arc lamp flow cytometer where CVs of less than 7% and as low as 3.2% are normally achieved. Determination of the S phase using these techniques produces a high correlation with DNA synthesis determined by radiolabeled precursor determination. These techniques permit the use of flow cytometry to determine proliferation during B cell activation.
Subject(s)
B-Lymphocytes/cytology , Benzimidazoles/radiation effects , Cell Cycle , Cell Survival , Ethidium/radiation effects , Flow Cytometry , Ultraviolet Rays , Animals , Cell Division , Cells, Cultured , DNA/analysis , Female , Intercalating Agents/radiation effects , Mice , Mice, Inbred C57BL , RNA/analysisABSTRACT
The role that potassium channels play in human T lymphocyte activation has been investigated by using specific potassium channel probes. Charybdotoxin (ChTX), a blocker of small conductance Ca(2+)-activated potassium channels (PK,Ca) and voltage-gated potassium channels (PK,V) that are present in human T cells, inhibits the activation of these cells. ChTX blocks T cell activation induced by signals (e.g., anti-CD2, anti-CD3, ionomycin) that elicit a rise in intracellular calcium ([Ca2+]i) by preventing the elevation of [Ca2+]i in a dose-dependent manner. However, ChTX has no effect on the activation pathways (e.g., anti-CD28, interleukin 2 [IL-2]) that are independent of a rise in [Ca2+]i. In the former case, both proliferative response and lymphokine production (IL-2 and interferon gamma) are inhibited by ChTX. The inhibitory effect of ChTX can be demonstrated when added simultaneously, or up to 4 h after the addition of the stimulants. Since ChTX inhibits both PK,Ca and PK,V, we investigated which channel is responsible for these immunosuppressive effects with the use of two other peptides, noxiustoxin (NxTX) and margatoxin (MgTX), which are specific for PK,V. These studies demonstrate that, similar to ChTX, both NxTX and MgTX inhibit lymphokine production and the rise in [Ca2+]i. Taken together, these data provide evidence that blockade of PK,V affects the Ca(2+)-dependent pathways involved in T lymphocyte proliferation and lymphokine production by diminishing the rise in [Ca2+]i that occurs upon T cell activation.
Subject(s)
Calcium/pharmacology , Ion Channel Gating/physiology , Lymphocyte Activation/physiology , Potassium Channels/physiology , T-Lymphocytes/physiology , Calcium/metabolism , Cell Division/drug effects , Cells, Cultured , Charybdotoxin , Dose-Response Relationship, Drug , Humans , Immunosuppressive Agents/pharmacology , Inositol 1,4,5-Trisphosphate/metabolism , Ionomycin/pharmacology , Lymphocyte Activation/drug effects , Lymphokines/metabolism , Mitogens/pharmacology , Neurotoxins/pharmacology , Scorpion Venoms/pharmacology , T-Lymphocytes/cytology , T-Lymphocytes/drug effectsABSTRACT
The development of autoimmune diabetes in the nonobese diabetic (NOD) mouse is controlled by multiple genes. At least one diabetogenic gene is linked to the major histocompatibility complex (MHC) of the NOD and is most likely represented by the two genes encoding the alpha and beta chains of the unique NOD class II molecule. Three other diabetogenic loci have recently been identified in the NOD mouse and are located on chromosomes 1, 3, and 11. In addition to the autoimmune diabetes which is caused by destruction of the insulin-producing beta cells in the pancreas, other manifestations of autoimmunity are seen in the NOD mouse. These include mononuclear cell inflammation of the submandibular and lacrimal glands, as well as the presence of circulating autoantibodies. To determine the effect of the non-MHC diabetogenic genes on the development of autoimmunity, we constructed the NOD.B10-H-2b (NOD.H-2b) strain, which possesses the non-MHC diabetogenic genes from the NOD mouse, but derives its MHC from the C57BL/10 (B10) strain. The NOD.H-2b strain does not develop insulitis, cyclophosphamide-induced diabetes, or spontaneous diabetes. It does, however, develop extensive lymphocytic infiltrates in the pancreas and the submandibular glands that are primarily composed of Thy 1.2+ T cells and B220+ B cells. In addition, autoantibodies are present in NOD.H-2b mice which recognize the "polar antigen" on the insulin-secreting rat tumor line RINm38. These observations demonstrate that the non-MHC genes in the NOD strain, in the absence of the NOD MHC, significantly contribute to the development of autoimmunity. The contribution of a single dose of the NOD MHC to autoimmunity was assessed with a (NOD x NOD.H-2b)F1 cross. Although only approximately 3% of F1 females developed spontaneous diabetes, approximately 50% of both female and male F1 mice developed insulitis, and 25% of females and 17% of males became diabetic after treatment with cyclophosphamide. These data demonstrate that the MHC-linked diabetogenic genes of the NOD mouse are dominant with decreasing levels of penetrance for the following phenotypes: insulitis greater than cyclophosphamide-induced diabetes greater than spontaneous diabetes.
Subject(s)
Autoimmune Diseases/genetics , Diabetes Mellitus, Type 1/genetics , Major Histocompatibility Complex , Mice, Inbred NOD/genetics , Pancreatitis/genetics , Animals , Autoantibodies/analysis , Cyclophosphamide/toxicity , Female , Male , Mice , Mice, Inbred C57BL , Pancreas/pathology , Pancreatitis/pathology , Submandibular Gland/pathologySubject(s)
Antigens, Differentiation, T-Lymphocyte/biosynthesis , Diabetes Mellitus, Type 1/immunology , H-2 Antigens/biosynthesis , Animals , B-Lymphocytes/immunology , Blotting, Northern , Disease Models, Animal , Flow Cytometry , Mice , Mice, Inbred BALB C , Mice, Inbred NOD , T-Lymphocytes/immunology , Tumor Necrosis Factor Receptor Superfamily, Member 7ABSTRACT
This report compares the ability of cyclosporin A and FK-506 to inhibit human T cell activation triggered via cell surface molecules that utilize different intracellular processes. We stimulated highly purified peripheral blood T lymphocytes with mitogens (Con A and PHA), ionomycin + PMA, or monoclonal antibodies specific for cell surface antigens involved in activation (CD2, CD3, CD28) either in combination with each other or in conjunction with PMA. Using measurements of the proliferative response, IL-2 production, and changes in intracellular Ca2+ ([Ca2+]i), we demonstrate that FK-506 exerts its inhibitory effect on early events of T-cell activation in a manner indistinguishable from that of CsA. An important finding in this study is the strict correlation between those activation pathways that are inhibited by FK-506 and CsA and the requirement that the sensitive pathways induce a measurable rise in [Ca2+]i. This correlation held even for the CD28/CD2 pathway which was previously shown to be calcium-independent; however by employing FACS analysis of [Ca2+]i within individual cells, a subset of cells activated via CD28/CD2 was found to respond with a measurable rise in [Ca2+]i. We also noted that the proliferative response induced by certain stimuli, such as ionomycin + PMA and PHA + PMA, was partially resistant to FK-506 and CsA, while IL-2 production was completely suppressed. The partial FK-506/CsA-resistance of these responses was shown to be determined by the amount of PMA added to the cultures. We conclude from our investigations that FK-506 and CsA inhibit highly similar signal transduction pathways in human T lymphocytes.
Subject(s)
Anti-Bacterial Agents/pharmacology , Cyclosporins/pharmacology , Immunosuppressive Agents/pharmacology , Signal Transduction/drug effects , T-Lymphocytes/drug effects , Antigens, Differentiation, T-Lymphocyte/physiology , CD2 Antigens , CD28 Antigens , CD3 Complex , Calcium/metabolism , Cells, Cultured , Humans , Interleukin-2/biosynthesis , Lymphocyte Activation/drug effects , Receptors, Antigen, T-Cell/physiology , Receptors, Immunologic/physiology , T-Lymphocytes/immunology , Tacrolimus , Tetradecanoylphorbol Acetate/pharmacologyABSTRACT
The effects of the immunosuppressants cyclosporin A (CsA), FK506 and rapamycin have been compared using murine B cells activated with a variety of mitogens. FK506 is a macrolide antibiotic that has been recently shown to inhibit T cell activation by a mechanism that appears similar to that of CsA. Rapamycin is a macrolide structurally related to FK506 whose mechanism of T cell suppression appears to be distinct from that of FK506 and CsA. While CsA and FK506 were found to preferentially inhibit B cell activation caused by stimuli which induce a rise in intracellular calcium, rapamycin partially inhibited activation by all stimuli tested, including those which are not associated with a calcium flux. All three compounds were found to inhibit cell cycle progression within the G1 phase; however, the rapamycin-sensitive event within G1 was completed earlier than the G1 events inhibited by CsA and FK506. In addition, inhibition of anti-IgM-activated B cells with CsA and FK506, but not with rapamycin, resulted in cell death. These data suggest that although CsA, FK506 and rapamycin are all inhibitors of B cell activation, the inhibitory activity of rapamycin can be clearly distinguished from that of CsA and FK506. Although the suppressive effects of CsA and FK506 on B cell proliferation were nearly identical in this study, their biological activities were distinguishable since FK506, but not CsA, could antagonize rapamycin-mediated suppression.
Subject(s)
Anti-Bacterial Agents/pharmacology , B-Lymphocytes/drug effects , Cyclosporins/pharmacology , Immunosuppressive Agents/pharmacology , Lymphocyte Activation/drug effects , Animals , Antibodies, Monoclonal , B-Lymphocytes/immunology , Cell Cycle/drug effects , Cell Survival/drug effects , Female , Histocompatibility Antigens Class II/immunology , Kinetics , Mice , Mice, Inbred C57BL , Polyenes/pharmacology , Sirolimus , TacrolimusABSTRACT
A steroidal glycolipid that enhances the nonspecific cellular response to opportunistic infection in an immunocompromised host has been discovered. A dose dependent response with 6-(5-cholesten-3 beta-yloxy)hexyl 1-thio-beta-D-mannopyranoside, L-644,257, was observed against several infective agents including bacterial, fungal, and viral pathogens in cyclophosphamide-treated mice. A mechanism for this protective action is proposed.
Subject(s)
Adjuvants, Immunologic/pharmacology , Cholesterol/analogs & derivatives , Immunity, Innate/drug effects , Adjuvants, Immunologic/administration & dosage , Administration, Oral , Animals , Candidiasis/immunology , Cholesterol/administration & dosage , Cholesterol/pharmacology , Female , Injections, Intramuscular , Injections, Intraperitoneal , Injections, Subcutaneous , Mice , Pseudomonas Infections/immunology , Staphylococcal Infections/immunology , Time FactorsABSTRACT
5-Halo-6-phenyl pyrimidinones, represented by 2-amino-5-bromo-6-phenyl-4(3H)-pyrimidinone (ABPP) and 2-amino-5-iodo-6-phenyl-4(3H)-pyrimidinone (AIPP), and 8-substituted guanosines, represented by 8-bromoguanosine (8-BrGuo) and 8-mercaptoguanosine (8-MGuo), are well-documented biological response modifiers. We have found that these substituted pyrimidinones and guanosines are very similar in their abilities to activate B cells. ABPP, AIPP, 8-BrGuo, and 8-MGuo induced murine B cells to polyclonally proliferate and differentiate in vitro. The maximal B-cell response levels and the kinetics of the responses elicited with both classes of compounds were comparable; however, ABPP and AIPP were approximately 10-fold more potent than 8-BrGuo and 8-MGuo. An additional similarity observed between the two classes was that polyclonal activation of B cells by ABPP, AIPP, 8-BrGuo, and 8-MGuo was limited to large B cells which had probably been activated previously in vivo. This is in contrast to lipopolysaccharide which is capable of inducing both large, activated B cells and small, resting B cells to proliferate and differentiate. Although substituted pyrimidinones and guanosines were not able to induce new DNA synthesis or antibody production in small B cells, both classes of compounds increased the expression of Ia antigens on the surface of both small and large B cells. These data, together with the recent observations that 8-BrGuo, like ABPP and AIPP, can stimulate NK and cytotoxic macrophage activity via the induction of interferon, strongly suggest that 5-halo-6-phenyl pyrimidinones and 8-substituted guanosines belong to the same structural class of biological response modifiers. Thus, the residues held in common by these two classes of stimulators may interact with the same cellular constituent in the target cells.
Subject(s)
B-Lymphocytes/drug effects , Cytosine/analogs & derivatives , Guanosine/analogs & derivatives , Thionucleosides/pharmacology , Animals , B-Lymphocytes/classification , B-Lymphocytes/cytology , Cell Differentiation/drug effects , Cytosine/pharmacology , Female , Guanosine/pharmacology , Histocompatibility Antigens Class II/analysis , Interphase/drug effects , Lymphocyte Activation/drug effects , Mice , Mice, Inbred C57BLABSTRACT
The murine Ly-6A.2 and Ly-6E.1 antigens, which can transduce triggering signals in T cells, have been shown to become highly expressed after mitogenic stimulation. It has recently been found that enhanced expression of Ly-6A/E antigens is also induced by interferon-gamma (IFN-gamma) in resting T cells. Here, the possibility is investigated that Ly-6A/E induction on activated T cells may be due to the IFN-gamma known to be secreted by these cells. A potent neutralizing anti-IFN-gamma monoclonal antibody (mAb) (H-22.10) was used. This mAb was found to abrogate the augmentation of Ly-6A/E antigens produced in resting T cells by supernatants from T cells stimulated with concanavalin A. When added directly into cultures of T cells stimulated with concanavalin A or by the combination of ionomycin with the protein kinase C activator phorbol myristate acetate (PMA), the H-22.10 mAb inhibited Ly-6A/E enhancement without affecting the blastogenesis or the emergence of interleukin 2 receptors and transferrin receptors. Such a selective effect of the anti-IFN-gamma mAb indicated that IFN-gamma is involved in the up-regulation of Ly-6A/E antigens during T cell activation. In determining whether other activation signals, in addition to IFN-gamma receptor occupancy, may contribute to Ly-6A/E enhancement, it was found that suboptimal stimulation of BALB/c T cells provided by a 3-hr pulse with ionomycin plus PMA or by culture with PMA alone potentiated by about twofold the increase of Ly-6E.1 induced by exogenous IFN-gamma. Therefore, Ly-6A/E augmentation in activated T cells reflects primarily an action of endogenous IFN-gamma that is amplified (in BALB/c mice) by a protein kinase C-dependent step.
Subject(s)
Antigens, Ly/biosynthesis , Interferon-gamma/physiology , T-Lymphocytes/immunology , Animals , Antibodies, Monoclonal/immunology , Concanavalin A/pharmacology , Ethers/pharmacology , Female , Gene Expression Regulation , Interferon-gamma/immunology , Ionomycin , Lymphocyte Activation/drug effects , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , T-Lymphocytes/metabolism , Tetradecanoylphorbol Acetate/pharmacologyABSTRACT
Previous studies have documented the ability of 8-bromoguanosine (8-BrGuo) and 8-mercaptoguanosine (8-MGuo) to induce polyclonal proliferation and differentiation of B cells from a variety of mouse strains. In the present study, we have defined the cellular target of this mitogenic activity. Using B cells fractionated according to size, we have found that large B cells are responsive to 8-BrGuo- and 8-MGuo-induced proliferation and differentiation whereas small, resting B cells are relatively unresponsive to these compounds. Addition of splenic adherent cells to the small B-cell fraction partially restored the proliferative but not the differentiative responses to 8-BrGuo and 8-MGuo. Although small B cells alone did not proliferate or differentiate in response to 8-BrGuo and 8-MGuo, cell surface expression of Ia antigens increased following incubation with these compounds. Thus, the biological activity of 8-BrGuo and 8-MGuo appears to be dictated by the cell type upon which it is acting. Small B cells are activated as evidenced by increased levels of surface Ia whereas large B cells are not only activated but are also induced to proliferate and differentiate.
Subject(s)
Antibody Formation , B-Lymphocytes/drug effects , Guanosine/analogs & derivatives , Lymphocyte Activation/drug effects , Thionucleosides/pharmacology , Animals , Antibodies, Anti-Idiotypic/immunology , Antigens, Surface/immunology , B-Lymphocytes/cytology , Cell Differentiation/drug effects , Cell Division/drug effects , Guanosine/pharmacology , Histocompatibility Antigens Class II/immunology , Macrophages/physiology , Mice , Muramidase/immunology , Receptors, Antigen, B-Cell/physiology , Spleen/cytologyABSTRACT
Trypanosoma cruzi trypomastigotes occur as a mixture of morphologically distinct forms in natural and experimental infections. We have been able to separate mixed populations into slender and broad forms using a continuous gradient composed of 12.5-25.0% hypaque in culture media. Previously, comparisons could only be made by using various strains of T. cruzi which show a predominance of either form. This separation procedure gave recoveries of 88-98% and had no effect on the viability of the parasites. Infectivity studies showed that slender forms were much more infectious to cultured muscle cells than were broad forms. However, studies with macrophages from CFW mice showed both forms to be equally capable of infection. It is concluded that the mechanism by which each form infects host cells is different; slender forms being capable of infecting both by penetration and by phagocytosis, while broad forms are only capable of infecting through phagocytosis.
Subject(s)
Trypanosoma cruzi/isolation & purification , Animals , Cells, Cultured , Centrifugation, Density Gradient , Macrophages/parasitology , Muscles/parasitology , Phagocytosis , Trypanosoma cruzi/cytology , Trypanosoma cruzi/physiologyABSTRACT
The separation of extracellular protozoan parasites from host cells based on a difference in surface charge has been described. However, with Trypanosoma cruzi no method exists for the isolation of pure parasite stages from heterogeneous mixtures. Studies on the electrophoresis of mixed stage populations confirm significant surface charge density differences exist among epimastigotes, trypomastigotes, and amastigotes. In ascending order of electronegativity, amastigotes have the lowest charge density, trypomastigotes next, followed by epimastigotes. A technique has been developed for the separation of purified populations of parasites based on these charge differences using a continuous free-flow electrophoresis apparatus. The separated populations are morphologically intact and maintain their infectivity to mice. This separation method is applicable for preparative and analytical isolation of pure stages of T. cruzi for biochemical and immunological studies.
Subject(s)
Trypanosoma cruzi/isolation & purification , Animals , Electrophoresis , Trypanosoma cruzi/growth & developmentSubject(s)
Spermatozoa/analysis , Animals , Cattle , Cell Membrane Permeability/drug effects , Detergents/pharmacology , Electrochemistry , Hydrogen-Ion Concentration , Isoelectric Focusing , Male , Phospholipids/analysis , Rabbits , Sperm Maturation , Spermatozoa/drug effects , Spin Labels , Surface Properties , Water/analysisABSTRACT
Changes induced in the ionic composition of the surface of herpes simplex virus (HSV) infected cells were evaluated by whole cell electrophoresis and isoelectric focusing. The kinetics of the progressive decline in cell electrophoretic mobility (a measurement of negative surface charge density) after infection corresponded to the production of infection virus. The observed HSV-induced increase in cell isoelectric focusing pH demonstrated that the electrokinetic cell surface became ionically more positive. The nature of the ionic changes were investigated using a series of specific reagents in conjunction with cell electrophoresis. Infected cells had 2.4 times surface binding sites for the amino-specific reagent 4-acetamide-4'-isothiocyanostilbene-2,2'-disulphonic acid. A relationship between these virus-induced amino groups and HSV surface antigens was indicated by the similar kinetics of their appearance and electrophoretic neutralization by HSV antibodies.
Subject(s)
Cell Membrane/physiology , Simplexvirus/growth & development , Antigens, Viral/analysis , Cell Membrane/drug effects , Cell Membrane/immunology , Cells, Cultured , Electrophoresis , Epitopes , Isoelectric Focusing , Simplexvirus/immunology , Stilbenes/pharmacology , Virus ReplicationABSTRACT
The application of cell electrophoresis to cytodiagnosis requires that a scientifically established basis exists for identifying abnormal cells electrophoretically, that research to detect such differences in the cytodiagnostic setting is possible and that a rapid and simple method of cell electrophoresis is adaptable to the clinical setting. Data are presented indicating modifications of electrophoretic mobility due to herpes simplex virus type 1 infection and Rous sarcoma virus transformation of culture cells. A simple apparatus for electrophoretically separating cells on a density gradient and collecting them for subsequent analysis is described, and results of experiments with this apparatus are consistent with those obtained by microscopic electrophoresis. Laser-doppler spectroscopic electrophoresis is suggested as a rapid method adaptable to clinical application.