ABSTRACT
[This corrects the article DOI: 10.1021/acscentsci.2c01100.].
ABSTRACT
Chemical modifications to DNA bases, including DNA adducts arising from reactions with electrophilic chemicals, are well-known to impact cell growth, miscode during replication, and influence disease etiology. However, knowledge of how genomic sequences and structures influence the accumulation of alkylated DNA bases is not broadly characterized with high resolution, nor have these patterns been linked with overall quantities of modified bases in the genome. For benzo(a) pyrene (BaP), a ubiquitous environmental carcinogen, we developed a single-nucleotide resolution damage sequencing method to map in a human lung cell line the main mutagenic adduct arising from BaP. Furthermore, we combined this analysis with quantitative mass spectrometry to evaluate the dose-response profile of adduct formation. By comparing damage abundance with DNase hypersensitive sites, transcription levels, and other genome annotation data, we found that although overall adduct levels rose with increasing chemical exposure concentration, genomic distribution patterns consistently correlated with chromatin state and transcriptional status. Moreover, due to the single nucleotide resolution characteristics of this DNA damage map, we could determine preferred DNA triad sequence contexts for alkylation accumulation, revealing a characteristic DNA damage signature. This new BaP damage signature had a profile highly similar to mutational signatures identified previously in lung cancer genomes from smokers. Thus, these data provide insight on how genomic features shape the accumulation of alkylation products in the genome and predictive strategies for linking single-nucleotide resolution in vitro damage maps with human cancer mutations.
ABSTRACT
Capillary electrophoresis, coupled with DNA 5' Texas Red labeling, was used to investigate the ability of MNase, FeII peplomycin, and duocarmycinâ B2 to access the nucleosome. Distinct accessibility patterns of these species to the nucleosome were observed. MNase was completely prevented from approaching the nucleosome core and exhibited a higher site specificity for targeting DNA sites located close to the core region. Intercalation of peplomycin in the nucleosomal core region was highly suppressed, but reaction sites located at the ends of the nucleosomal core remained accessible, which implied flexibility of the core DNA end. Duocarmycinâ B2 was able to enter and react in the core region, although its alkylating efficiency decreased significantly.
Subject(s)
Ferrous Compounds/chemistry , Indoles/chemistry , Micrococcal Nuclease/chemistry , Nucleosomes/chemistry , Peplomycin/chemistry , DNA/chemistry , DNA Cleavage/drug effects , Duocarmycins , Electrophoresis, Capillary , Pyrrolidinones/chemistryABSTRACT
Given that our knowledge of DNA repair is limited because of the complexity of the DNA system, a technique called UVA micro-irradiation has been developed that can be used to visualize the recruitment of DNA repair proteins at double-strand break (DSB) sites. Interestingly, Hoechst 33258 was used under micro-irradiation to sensitize 5-bromouracil (BrU)-labelled DNA, causing efficient DSBs. However, the molecular basis of DSB formation under UVA micro-irradiation remains unknown. Herein, we investigated the mechanism of DSB formation under UVA micro-irradiation conditions. Our results suggest that the generation of a uracil-5-yl radical through electron transfer from Hoechst 33258 to BrU caused DNA cleavage preferentially at self-complementary 5'-AABrUBrU-3' sequences to induce DSB. We also investigated the DNA cleavage in the context of the nucleosome to gain a better understanding of UVA micro-irradiation in a cell-like model. We found that DNA cleavage occurred in both core and linker DNA regions although its efficiency reduced in core DNA.
Subject(s)
Bisbenzimidazole/pharmacology , Bromouracil/chemistry , DNA/drug effects , Ultraviolet Rays , Bisbenzimidazole/chemistry , DNA Breaks, Double-Stranded/drug effects , DNA Cleavage/drug effects , Dose-Response Relationship, Drug , Free Radicals/chemistry , Molecular Structure , Structure-Activity RelationshipABSTRACT
Tet (ten-eleven translocation) family proteins oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC), and are suggested to be involved in the active DNA demethylation pathway. In this study, we reconstituted positioned mononucleosomes using CpG-methylated 382â bp DNA containing the Widom 601 sequence and recombinant histone octamer, and subjected the nucleosome to treatment with Tet1 protein. The sites of oxidized methylcytosine were identified by bisulfite sequencing. We found that, for the oxidation reaction, Tet1 protein prefers mCs located in the linker region of the nucleosome compared with those located in the core region.
Subject(s)
5-Methylcytosine/analogs & derivatives , 5-Methylcytosine/chemistry , Cytosine/analogs & derivatives , DNA/metabolism , Nucleosomes/chemistry , Cytosine/chemistry , DNA Methylation , Nucleosomes/metabolism , Oxidation-ReductionABSTRACT
Functional cooperativity among transcription factors on regulatory genetic elements is pivotal for milestone decision-making in various cellular processes including mammalian development. However, their molecular interaction during the cooperative binding cannot be precisely understood due to lack of efficient tools for the analyses of protein-DNA interaction in the transcription complex. Here, we demonstrate that photoinduced excess electron transfer assay can be used for analysing cooperativity of proteins in transcription complex using cooperative binding of Pax6 to Sox2 on the regulatory DNA element (DC5 enhancer) as an example. In this assay, (Br)U-labelled DC5 was introduced for the efficient detection of transferred electrons from Sox2 and Pax6 to the DNA, and guanine base in the complementary strand was replaced with hypoxanthine (I) to block intra-strand electron transfer at the Sox2-binding site. By examining DNA cleavage occurred as a result of the electron transfer process, from tryptophan residues of Sox2 and Pax6 to DNA after irradiation at 280 nm, we not only confirmed their binding to DNA but also observed their increased occupancy on DC5 with respect to that of Sox2 and Pax6 alone as a result of their cooperative interaction.
Subject(s)
Electrons , Enhancer Elements, Genetic , PAX6 Transcription Factor/metabolism , SOXB1 Transcription Factors/metabolism , Base Sequence , Bromouracil/analogs & derivatives , DNA/metabolism , DNA Cleavage/radiation effects , Humans , Hypoxanthine/metabolism , Light , PAX6 Transcription Factor/chemistry , Protein Binding/radiation effects , Protein Domains , Protein Structure, Secondary , Reproducibility of Results , SOXB1 Transcription Factors/chemistry , Spectrometry, Fluorescence , Structure-Activity Relationship , Tryptophan/metabolism , Uridine/analogs & derivatives , Uridine/metabolismABSTRACT
To evaluate the reactivity of antitumor agents in a nucleosome architecture, we conducted in vitro studies to assess the alkylation level of duocarmycin B2 on nucleosomes with core and linker DNA using sequencing gel electrophoresis. Our results suggested that the alkylating efficiencies of duocarmycin B2 were significantly decreased in core DNA and increased at the histone-free linker DNA sites when compared with naked DNA conditions. Our finding that nucleosome assembly alters the accessibility of duocarmycin B2 to duplex DNA could advance its design as an antitumor agent.
Subject(s)
Antineoplastic Agents/chemistry , DNA/chemistry , Indoles/chemistry , Alkylation , Antineoplastic Agents/metabolism , Base Sequence , DNA/metabolism , Duocarmycins , Indoles/metabolism , Nucleosomes/metabolism , Pyrrolidinones/chemistry , Pyrrolidinones/metabolismABSTRACT
Tet (ten-eleven translocation) family proteins have the ability to oxidize 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC). However, the oxidation reaction of Tet is not understood completely. Evaluation of genomic-level epigenetic changes by Tet protein requires unbiased identification of the highly selective oxidation sites. In this study, we used high-throughput sequencing to investigate the sequence specificity of mC oxidation by Tet1. A 6.6×10(4) -member mC-containing random DNA-sequence library was constructed. The library was subjected to Tet-reactive pulldown followed by high-throughput sequencing. Analysis of the obtained sequence data identified the Tet1-reactive sequences. We identified mCpG as a highly reactive sequence of Tet1 protein.
Subject(s)
5-Methylcytosine/chemistry , High-Throughput Screening Assays , Mixed Function Oxygenases/chemistry , Proto-Oncogene Proteins/chemistry , Oxidation-ReductionABSTRACT
We report the photochemistry of (Br)U substituted DNA as a versatile platform to investigate the binding sites of pyrene conjugated pyrrole imidazole polyamides (PIPs). The results suggest that the approach can be used on a routine basis for the screening of polyamide binding sites.
Subject(s)
Bromouracil/chemistry , DNA/chemistry , Nylons/chemistry , Binding Sites , ElectronsABSTRACT
Ultraviolet (UV) radiation causes cellular DNA damage, among which cyclobutane pyrimidine dimers (CPDs) are responsible for a variety of genetic mutations. Although several approaches have been developed for detection of CPDs, conventional methods require time-consuming steps. Aquaphotomics, a new approach based on near-infrared spectroscopy (NIRS) and multivariate analysis that determines interactions between water and other components of the solution, has become an effective method for qualitative and quantitative parameters measurement in the solutions. NIR spectral patterns of UVC-irradiated and nonirradiated DNA solutions were evaluated using aquaphotomics for detection of UV-induced CPDs. Groups of UV-irradiated and nonirradiated DNA samples were classified (87.5% accuracy) by soft independent modeling of class analogy (SIMCA). A precise regression model calculated from NIR water spectral patterns based on UVC doses (r Val = 0.9457) and the concentration of cis-syn cyclobutane thymine dimers (cis-syn T<>Ts; r Val = 0.9993) was developed using partial least squares regression (PLSR), while taking advantage of water spectral patterns, particularly around 1400-1500 nm. Our results suggested that, in contrast to DNA, the formation of cis-syn T<>Ts increased the strongly hydrogen bonded water. Additionally, NIRS could qualitatively and quantitatively detect cis-syn T<>Ts in isolated DNA aqueous solutions upon UVC exposure.
Subject(s)
DNA Damage/radiation effects , DNA/radiation effects , Pyrimidine Dimers/isolation & purification , Ultraviolet Rays , Mutagenesis/radiation effects , Mutation/radiation effects , Pyrimidine Dimers/radiation effects , Spectroscopy, Near-InfraredABSTRACT
Next-generation-sequencing (NGS) technologies enable us to obtain extensive information by deciphering millions of individual DNA sequencing reactions simultaneously. The new DNA-sequencing strategies exceed their precursors in output by many orders of magnitude, resulting in a quantitative increase in valuable sequence information that could be harnessed for qualitative analysis. Sequencing on this scale has facilitated significant advances in diverse disciplines, ranging from the discovery, design, and evaluation of many small molecules and relevant biological mechanisms to maturation of personalized therapies. NGS technologies that have recently become affordable allow us to gain in-depth insight into small-molecule-triggered biological phenomena and empower researchers to develop advanced versions of small molecules. In this review we focus on the overlooked implications of NGS technologies in chemical biology, with a special emphasis on small-molecule development and screening.
Subject(s)
Drug Discovery , Genome, Human , High-Throughput Nucleotide Sequencing/methods , Small Molecule Libraries/pharmacology , Biochemistry , Computational Biology , High-Throughput Nucleotide Sequencing/instrumentation , High-Throughput Screening Assays , Humans , Nucleic Acid Conformation , Precision Medicine , Small Molecule Libraries/chemistryABSTRACT
The identification of binding sites for small molecules in genomic DNA is important in various applications. Previously, we demonstrated rapid transcriptional activation by our small molecule SAHA-PIP. However, it was not clear whether the strong biological effects exerted by SAHA-PIP were attributable to its binding specificity. Here, we used high-throughput sequencing (Bind-n-seq) to determine the binding specificity of SAHA-PIPs. Sequence specificity bias was determined for SAHA-PIPs (3 and 4), and this showed enhanced 6 bp sequence-specific binding compared with hairpin PIPs (1 and 2). This finding allowed us to investigate the role of the ß-alanine that links SAHA to PIP, and led in turn to the design of ßß-PIPs (5 and 6), which showed enhanced binding specificity. Overall, we demonstrated the importance of ß-moieties for the binding specificity of PIPs and the use of cost-effective high-throughput screening of these small molecules for binding to the DNA minor groove.
Subject(s)
DNA/metabolism , Imidazoles/chemistry , Nylons/chemistry , Pyrroles/chemistry , beta-Alanine/chemistry , Base Sequence , Binding Sites , DNA/chemistry , High-Throughput Nucleotide Sequencing , Imidazoles/metabolism , Nylons/metabolism , Pyrroles/metabolism , beta-Alanine/metabolismABSTRACT
In a previous study, we found that 2-deoxyribonolactone is effectively generated in the specific 5-bromouracil ((Br)U)-substituted sequence 5'-(G/C)[A]n = 1,2 (Br)U(Br)U-3' and proposed that a formed uracil-5-yl radical mainly abstracts the C1' hydrogen from the 5'-side of (Br)U(Br)U under 302-nm irradiation condition. In the present work, we performed photoirradiation of (Br)U-substituted DNA in the presence of a hydrogen donor, tetrahydrofuran, to quench the uracil-5-yl radical to uracil and then subjected the sample to uracil DNA glycosylase digestion. Slab gel sequence analysis indicated that uracil residues were formed at the hot-spot sequence of 5'-(G/C)[A]n = 1,2 (Br)U(Br)U-3' in 302-nm irradiation of (Br)U-substituted DNA. Furthermore, we found that the uracil residue was also formed at the reverse sequence 5'-(Br)U(Br)U[A]n = 1,2(G/C)-3', which suggests that both 5'-(G/C)[A]n = 1,2 (Br)U(Br)U-3' and 5'-(Br)U(Br)U[A]n = 1,2(G/C)-3' are hot-spot sequences for the formation of the uracil-5-yl radical.
Subject(s)
Bromouracil/chemistry , DNA/chemistry , Sequence Analysis, DNA/methods , Uracil/chemistry , DNA/radiation effects , Electrophoresis, Polyacrylamide Gel , Furans/chemistry , Ultraviolet Rays , Uracil-DNA GlycosidaseABSTRACT
Introducing novel building blocks to solid-phase peptide synthesis, we readily synthesized long-chain hairpin pyrrole-imidazole (PI) polyamide-chlorambucil conjugates 3 and 4 via the introduction of an amino group into a GABA (γ-turn) contained in 3, to target CAG/CTG repeat sequences, which are associated with various hereditary disorders. A high-resolution denaturing polyacrylamide sequencing gel revealed sequence-specific alkylation both strands at the N3 of adenines or guanines in CAG/CTG repeats by conjugates 3 and 4, with 11bp recognition. In vitro transcription assays using conjugate 4 revealed that specific alkylation inhibited the progression of RNA polymerase at the alkylating sites. Chiral substitution of the γ-turn with an amino group resulted in higher binding affinity observed in SPR assays. These assays suggest that conjugates 4 with 11bp recognition has the potential to cause specific DNA damage and transcriptional inhibition at the alkylating sites.
Subject(s)
Chlorambucil/pharmacology , DNA/drug effects , Imidazoles/pharmacology , Nylons/pharmacology , Pyrroles/pharmacology , Transcription, Genetic/drug effects , Trinucleotide Repeats/genetics , Alkylation/drug effects , Chlorambucil/chemistry , DNA/genetics , DNA/metabolism , Imidazoles/chemistry , Nylons/chemistry , Pyrroles/chemistry , Structure-Activity Relationship , Trinucleotide Repeats/drug effectsABSTRACT
Tet family proteins have the ability to convert 5-methylcytosine (mC) to 5-hydroxymethylcytosine, and further to 5-formylcytosine and 5-carboxycytosine. We found that CGmCGCG can be the substrate of Tet protein, and observed iterative oxidation of mC by HPLC analysis. We also demonstrated that Tet protein favours single-stranded DNA over double-stranded DNA.
Subject(s)
5-Methylcytosine/metabolism , DNA-Binding Proteins/metabolism , Oligoribonucleotides/metabolism , 5-Methylcytosine/chemistry , DNA-Binding Proteins/chemistry , Oligoribonucleotides/chemistry , Oxidation-ReductionABSTRACT
Charge transfer through DNA is of great interest because of the potential of DNA to be a building block for nanoelectronic sensors and devices. The photochemical reaction of 5-halouracil has been used for probing charge-transfer processes along DNA. We previously reported on unique charge transfer following photochemical reaction of 5-bromouracil within four-base π-stacks in Z-DNA. In this study, we incorporated a guanosine instead of a deoxyguanosine into Z-DNA, and found that electron transfer occurs in a different mechanism through four-base π-stacks.
Subject(s)
Carbohydrates/chemistry , DNA, Z-Form/chemistry , Deoxyguanosine/chemistry , Ribonucleotides/chemistry , Bromouracil/chemistry , Electron Transport , Electrons , Quantum Theory , Ultraviolet RaysABSTRACT
The wrapping and unwrapping of the nucleosome, which is a fundamental packing unit of chromatin, are tied to the regulation of gene expression. The accessibility of DNA within nucleosomes is controlled not only by chromatin-remodeling molecules, but also by chemical modifications of histones and DNA. Understanding the structural changes of a nucleosome during epigenetic modifications is a key to unravel the mechanisms of gene regulation. Here, we reconstituted nucleosomes using methylcytosine- or hydroxymethylcytosine-substituted DNA, and analyzed their morphological features by atomic force microscopy (AFM). Our results indicate that cytosine methylation induces overwrapping of the DNA around the histone octamer, whereas cytosine hydroxymethylation has a lesser effect on the overwrapping of the DNA. These results suggest that two types of DNA modification yield different wrapping states of nucleosomes, which may contribute to the compaction and relaxation of the chromatin structure.
ABSTRACT
Electron transfer in DNA has been intensively studied to elucidate its biological roles and for applications in bottom-up DNA nanotechnology. Recently, mechanisms of electron transfer to DNA have been investigated; however, most of the systems designed are intramolecular. Here, we synthesized pyrene-conjugated pyrrole-imidazole polyamides (PPIs) to achieve sequence-specific electron injection into DNA in an intermolecular fashion. Electron injection from PPIs into DNA was detected using 5-bromouracil as an electron acceptor. Twelve different 5-bromouracil-containing oligomers were synthesized to examine the electron-injection ability of PPI. Product analysis demonstrated that the electron transfer from PPIs was localized in a range of 8 bp from the binding site of the PPIs. These results demonstrate that PPIs can be a useful tool for sequence-specific electron injection.
Subject(s)
DNA/chemistry , Electrons , Nylons/chemistry , Base Sequence , Bromouracil/chemistry , DNA/radiation effects , Imidazoles/chemistry , Nylons/chemical synthesis , Pyrenes/chemistry , Pyrroles/chemistryABSTRACT
5-Bromouracil ((Br)U) was incorporated into three types of synthetic RNA and the products of the photoirradiated (Br)U-containing RNAs were investigated using HPLC and MS analysis. The photoirradiation of r(GCA(Br)UGC)(2) and r(CGAA(Br)UUGC)/r(GCAAUUCG) in A-form RNA produced the corresponding 2'-keto adenosine ((keto)A) product at the 5'-neighboring nucleotide, such as r(GC(keto)AUGC) and r(CGA(keto)AUUGC), respectively. The photoirradiation of r(CGCG(Br)UGCG)/r(C(m)GCAC(m)GCG) in Z-form RNA produced the 2'-keto guanosine ((keto)G) product r(CGC(keto)GUGCG), whereas almost no products were observed from the photoirradiation of r(CGCG(Br)UGCG)/r(C(m)GCAC(m)GCG) in A-form RNA. The present results indicate clearly that hydrogen (H) abstraction by the photochemically generated uracil-5-yl radical selectively occurs at the C2' position to provide a 2'-keto RNA product.