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Objective To observe the efficacy of levofloxacin hydrochloride eye gel (LHEG) in treatment of Staphylococcus aureus keratitis in rabbits. Methods Sixty rabb received intrastromal injection of 10 μL bacterial suspension conta establish Staphylococcus aureus keratitis model. Forty-eight rabb into four groups; 24 h baseline group, phosphate buffer solution (PBS) control group, levofloxacin hydrochloride eye drop (LHED) group, and LHEG group. Rabbits in the 24 h baseline group received slit-lamp examination and were given clinical score, and their corneas were collected for histopathology examination and bacterial quantitation. The other three groups received treatment with PBS, LHED or LHEG three times a day for seven successive days. In the last day, rabbits in these three groups received the same examination as that in the 24 h baseline group. The clinical scores and cornea bacterial quantitation were analyzed with variance analysis, and a P value less than 0. 05 was considered as statistically significant Results Compared wth PBS control group, clinical symptoms of LHED group and LHEG group were significantly reduced (P = 0. 00) on the seventh day, with the clinical scores being (4. 21 ± 1. 10) and (3. 63±0. 86), respectively; the numbers of bacterial colonies were also significantly reduced in LHED group and LHEG group (P =0. 00), with the numbers being (4. 87 ± 0. 05) and (4. 64±0. 10) CFU, respectively. Moreover, LHEG group had a significantly better bacteriostatic effect than LHEG group (P =0. 00). Conclusion Local application of LHEG can greatly inhibk the growth of bacterial colony of Staphylococcus aureus and improve the clinical signs of keratitis, with the efficacy being better than that of routinely used LHED in rabbk model of Staphylococcus aureus keratitis.
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<p><b>OBJECTIVE</b>To access the sustained immune effect in influenza A H1N1 vaccine vaccinated-blood donors as well as the level of anti-H1N1 IgG in unvaccinated-blood donors in order to provide reference for preventing and treating influenza A H1N1.</p><p><b>METHODS</b>Anti-H1N1 IgG was detected in 1166 vaccinated-blood donors as well as 1265 unvaccinated-blood donors by ELISA method in Dongguan from January 2010 to June 2010.</p><p><b>RESULTS</b>The mean positive rate and high-titer rate of anti-H1N1 IgG were 78.82% and 46.57% respectively, both of which were sustained at relatively high level after reaching their peak at vaccination time of 71-90 d. The mean positive rate of anti-H1N1 IgG in unvaccinated-blood donors was 26.01%. No difference was found in the positive rate of anti-H1N1 IgG among different gender or age groups (P > 0.05).</p><p><b>CONCLUSION</b>The influenza A H1N1 vaccine, with good sustained immune effect, plays an important role in preventing and treating influenza A H1N1. The positive rate of anti-H1N1 IgG in influenza A HIN1 vaccine unvaccinated-blood donors is low. Vaccination should be strengthened.</p>
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Adolescente , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Anticuerpos Antivirales , Sangre , Alergia e Inmunología , Donantes de Sangre , Ensayo de Inmunoadsorción Enzimática , Inmunoglobulina G , Sangre , Alergia e Inmunología , Subtipo H1N1 del Virus de la Influenza A , Alergia e Inmunología , Vacunas contra la Influenza , Alergia e Inmunología , Gripe Humana , Sangre , Alergia e InmunologíaRESUMEN
<p><b>OBJECTIVE</b>To investigate the characteristics of seroconversion of HBV NAT screening-positive crowd from blood donors in Dongguan city and provide reference for the safety of blood transfusion and disease prevention.</p><p><b>METHODS</b>With retrospective survey, Nucleic acid testing (NAT) was used to analyze 28800 HBsAg-negative samples by ELISA from blood donors in Dongguan city from August, 2006 to August, 2007 with Roche Cobas AmpliScreen systems; and follow-up research including NAT for HBV-DNA, ELISA for HBsAg and multiple factors analysis for HBV infection was carried out on HBV NAT screening-positive crowd.</p><p><b>RESULTS</b>10 positive pooling were screened from 28800 samples; after further detection, 2 of these positive pooling were HBV-DNA negative and 8 HBV-DNA positive samples were found.The 10-week follow-up research on these 8 blood donors showed that 6 were HBV-DNA positive and HBsAg-negative at 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks respectively, 1 was not HBsAg positive until 2 weeks and was positive on follow-up, and considered in "window period", 1 was HBV-DNA negative, HBsAg-negative on follow-up. Of these 8,7 were not only migrant laborers with poor condition of work, life and health but also in high risk of secondary infection for HBV, in addition they had little idea of therapy or prevention measures of HBV infection and the other 1 was HBV-DNA negative, HBsAg-negative on follow-up, who was in better condition than the above 7 donors.</p><p><b>CONCLUSION</b>NAT is more sensitive than ELISA in screening HBV, but the probability of being false positive of NAT can not be ignored at the same time. On the hand, only screening HBsAg for HBV is relative limitation in high infection region of China. Some factors would have effect on the serum conversion of blood donors including the quality of work and life, therapy or prevention measures.</p>
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Adulto , Femenino , Humanos , Masculino , Adulto Joven , Donantes de Sangre , ADN Viral , Sangre , Genética , Ensayo de Inmunoadsorción Enzimática , Estudios de Seguimiento , Hepatitis B , Sangre , Diagnóstico , Antígenos e de la Hepatitis B , Sangre , Virus de la Hepatitis B , Genética , Alergia e Inmunología , Tamizaje Masivo , Métodos , Técnicas de Amplificación de Ácido Nucleico , Métodos , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
<p><b>OBJECTIVE</b>To understand the genetic polymorphism of DC-SIGN's and DC-SIGNR's neck regions in normal Chinese Han population, and to obtain the genetic data of the two loci in Chinese Han population.</p><p><b>METHODS</b>The genotypes and alleles of repeat sequences of DC-SIGN and DC-SIGNR neck region were typed by PCR, agarose gel electrophoresis and sequencing. Polymorphism information content (PIC) of DC-SIGNR was calculated.</p><p><b>RESULTS</b>DC-SIGN genetic polymorphism was rare. Allele 7 was most and its frequency was 0.9808. 4-, 5-, 6- and 8- alleles were also found, although their frequencies were very low. Caucasians had only 6- and 8- allele mutants; DC-SIGNR genetic polymorphism was high, its PIC was 0.5312, 4-,5-,6-,7-,8-,9- alleles and 16 genotypes were found in normal Chinese Han population. The differences of 6/5,7/4,7/5,7/6,7/7,9/5,9/7,9/9 genotypes distribution and 5-,6-,7-,9- alleles frequency between normal Chinese Han population and Caucasian population were all extremely distinct (P<0.01). The inserted mutation seemed more in Chinese Hans than Caucasian population.</p><p><b>CONCLUSION</b>DC-SIGN and DC-SIGNR genotypes and alleles distribution in Chinese Han population are significantly different from Caucasian population and with Chinese own population genetic characteristics, compared with Caucasians.</p>
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Adolescente , Adulto , Femenino , Humanos , Masculino , Adulto Joven , Alelos , Pueblo Asiatico , Genética , Moléculas de Adhesión Celular , Genética , China , Frecuencia de los Genes , Genotipo , Lectinas Tipo C , Genética , Reacción en Cadena de la Polimerasa , Polimorfismo Genético , Genética , Receptores de Superficie Celular , GenéticaRESUMEN
<p><b>BACKGROUND</b>To evaluate homemade and imported HbsAg ELISA kits on screening blood donors.</p><p><b>METHODS</b>Samples for evaluation included 120 HbsAg serum plates for the golden criteria and 400 sets of serum from blood donors in Dongguan. The samples underwent blind screening with homemade and imported ELISA kits respectively.</p><p><b>RESULTS</b>The sensitivity of homemade (Xinchuang) and imported (Diasorin) HbsAg ELISA kit were 85.71% (72/84) and 100% (84/84), respectively. Their specificity was 100% (436/436) and 96.55% (421/436) respectively. The consistency of two ELISA kits was 100%.</p><p><b>CONCLUSION</b>The imported ELISA kit had the highest sensitivity, but its specificity was not as good as that of homemade ELISA kit. The two kinds of ELISA kits had good repetition. The combination of the two reagents may ensure the safety of blood transfusion.</p>
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Humanos , Donantes de Sangre , China , Ensayo de Inmunoadsorción Enzimática , Métodos , Estándares de Referencia , Hepatitis B , Sangre , Diagnóstico , Antígenos de Superficie de la Hepatitis B , Sangre , Tamizaje Masivo , Métodos , Estándares de Referencia , Juego de Reactivos para Diagnóstico , Estándares de Referencia , Estándares de Referencia , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
The effects of continuous subcutaneous insulin injection (CSII, n=306) and multiple subcutaneous insulin injection (MSII, n=310)on hyperglycemia in patients with diabetes mellitus were compared. The results suggest that the effect of CSII is more prompt and efficient with lower incidence of hypoglycemia as compared with MSII.