RÉSUMÉ
Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron cryomicroscopy to enhance the image contrast of ice-embedded herpes simplex virus type 1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid and, for the first time, show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal.
Sujet(s)
Capside/ultrastructure , Herpèsvirus humain de type 1/ultrastructure , Animaux , Cryomicroscopie électronique/méthodes , Humains , Traitement d'image par ordinateur/méthodes , Microscopie de contraste de phaseRÉSUMÉ
Examination of the three-dimensional structure of intact herpes simplex virus type 1 (HSV-1) virions had revealed that the icosahedrally symmetrical interaction between the tegument and capsid involves the pentons but not the hexons (Z. H. Zhou, D. H. Chen, J. Jakana, F. J. Rixon, and W. Chiu, J. Virol. 73:3210-3218, 1999). To account for this, we postulated that the presence of the small capsid protein, VP26, on top of the hexons was masking potential binding sites and preventing tegument attachment. We have now tested this hypothesis by determining the structure of virions lacking VP26. Apart from the obvious absence of VP26 from the capsids, the structures of the VP26 minus and wild-type virions were essentially identical. Notably, they showed the same tegument attachment patterns, thereby demonstrating that VP26 is not responsible for the divergent tegument binding properties of pentons and hexons.
Sujet(s)
Protéines de capside , Capside/physiologie , Herpèsvirus humain de type 1/physiologie , Virion/physiologie , Séquence nucléotidique , Amorces ADN , Électrophorèse sur gel de polyacrylamide , Virion/composition chimiqueRÉSUMÉ
The dielectrophoretic behaviour of the capsids of herpes simplex virus type-1 has been measured over a range of conductivities of KCl solutions, with and without the addition of mannitol. The dielectrophoretic response of the capsids was recorded by measuring the frequency corresponding to zero dielectrophoretic force. The data were analysed using a multi-shelled model, and the permittivity and conductivity of the particles estimated. The capsid was modelled as a porous protein shell through which suspending medium passes, an inner chamber containing suspending medium in equilibrium with the outside, and a central core of protein (the scaffold). Capsids suspended in KCl without mannitol exhibited a different behaviour to those suspended in KCl with mannitol.
Sujet(s)
Capside/composition chimique , Électrophorèse/méthodes , Herpèsvirus humain de type 1/composition chimique , Phénomènes biophysiques , Biophysique , Capside/ultrastructure , Conductivité électrique , Électrochimie , Herpèsvirus humain de type 1/ultrastructure , Microscopie électronique , Chlorure de potassium , SolutionsRÉSUMÉ
Several factors, including spatial and temporal coherence of the electron microscope, specimen movement, recording medium, and scanner optics, contribute to the decay of the measured Fourier amplitude in electron image intensities. We approximate the combination of these factors as a single Gaussian envelope function, the width of which is described by a single experimental B-factor. We present an improved method for estimating this B-factor from individual micrographs by combining the use of X-ray solution scattering and numerical fitting to the average power spectrum of particle images. A statistical estimation from over 200 micrographs of herpes simplex virus type-1 capsids was used to estimate the spread in the experimental B-factor of the data set. The B-factor is experimentally shown to be dependent on the objective lens defocus setting of the microscope. The average B-factor, the X-ray scattering intensity of the specimen, and the number of particles required to determine the structure at a lower resolution can be used to estimate the minimum fold increase in the number of particles that would be required to extend a single particle reconstruction to a specified higher resolution. We conclude that microscope and imaging improvements to reduce the experimental B-factor will be critical for obtaining an atomic resolution structure.
Sujet(s)
Cryomicroscopie électronique/méthodes , Analyse de Fourier , Traitement d'image par ordinateur/méthodes , Capside/composition chimique , Capside/ultrastructure , Simulation numérique , Herpèsvirus humain de type 1/composition chimique , Herpèsvirus humain de type 1/ultrastructure , Structure quaternaire des protéines , Sensibilité et spécificité , Solutions , Diffraction des rayons XRÉSUMÉ
Human herpesviruses are large and structurally complex viruses that cause a variety of diseases. The three-dimensional structure of the herpesvirus capsid has been determined at 8.5 angstrom resolution by electron cryomicroscopy. More than 30 putative alpha helices were identified in the four proteins that make up the 0.2 billion-dalton shell. Some of these helices are located at domains that undergo conformational changes during capsid assembly and DNA packaging. The unique spatial arrangement of the heterotrimer at the local threefold positions accounts for the asymmetric interactions with adjacent capsid components and the unusual co-dependent folding of its subunits.
Sujet(s)
Capside/composition chimique , Capside/ultrastructure , Herpèsvirus humain de type 1/ultrastructure , Protéines de capside , Cryomicroscopie électronique , Herpèsvirus humain de type 1/composition chimique , Traitement d'image par ordinateur , Modèles moléculaires , Masse moléculaire , Conformation des protéines , Pliage des protéines , Structure quaternaire des protéines , Structure secondaire des protéines , Structure tertiaire des protéinesRÉSUMÉ
All members of the herpesvirus family have a characteristic virion structure, comprising a DNA containing, icosahedral capsid, embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope. Human cytomegalovirus (HCMV, the prototypic beta-herpesvirus) has a genome that is significantly larger (>50 %) than that of the alpha-herpesvirus HSV-1. Although the internal volume of the HCMV capsid is approximately 17 % larger than that of HSV-1, this slight increase in volume does not provide adequate space to encapsidate the full length HCMV genome at the same packing density as HSV-1. We have investigated the nature of DNA packing in HCMV and HSV-1 virions by electron-cryomicroscopy and image processing. Radial density profiles calculated from projection images of HCMV and HSV-1 capsids suggest that there is no increase in the volume of the HCMV capsid upon DNA packaging. Packing density of the viral DNA was assessed for both HCMV and HSV-1 by image analysis of both full and empty particles. Our results for packing density in HSV-1 are in good agreement with previously published measurements, showing an average inter-layer spacing of approximately 26 A. Measurements taken from our HCMV images, however, suggest that the viral genomic DNA is more densely packed, with an average inter-layer spacing of approximately 23 A. We propose therefore, that the combination of greater volume in HCMV capsids and increased packing density of viral DNA accounts for its ability to encapsidate a large genome.
Sujet(s)
Cytomegalovirus/génétique , ADN viral/génétique , Herpèsvirus humain de type 1/génétique , Microscopie électronique/méthodes , Virion/génétique , Cytomegalovirus/ultrastructure , Génome viral , Virion/ultrastructureRÉSUMÉ
Typical herpes simplex virus (HSV) capsids contain seven proteins that form a T=16 icosahedron of 1,250-A diameter. Infection of cells with recombinant baculoviruses expressing two of these proteins, VP5 (which forms the pentons and hexons in typical HSV capsids) and VP19C (a component of the triplexes that connect adjacent capsomeres), results in the formation of spherical particles of 880-A diameter. Electron cryomicroscopy and computer reconstruction revealed that these particles possess a T=7 icosahedral symmetry, having 12 pentons and 60 hexons. Among the characteristic structural features of the particle are the skewed appearance of the hexons and the presence of intercapsomeric mass densities connecting the middle domain of one hexon subunit to the lower domain of a subunit in the adjacent hexon. We interpret these connecting masses as being formed by VP19C. Comparison of the connecting masses with the triplexes, which occupy equivalent positions in the T=16 capsid, reveals the probable locations of the single VP19C and two VP23 molecules that make up the triplex. Their arrangement suggests that the two triplex proteins have different roles in controlling intercapsomeric interactions and capsid stability. The nature of these particles and of other aberrant forms made in the absence of scaffold demonstrates the conformational adaptability of the capsid proteins and illustrates how VP23 and the scaffolding protein modulate the nature of the VP5-VP19C network to ensure assembly of the functional T=16 capsid.
Sujet(s)
Capside/ultrastructure , Herpèsvirus humain de type 1/ultrastructure , Conformation des protéines , Capside/biosynthèse , Capside/génétique , Protéines de capside , Cryomicroscopie électronique , Herpèsvirus humain de type 1/génétique , Humains , Traitement d'image par ordinateur , Protéines de fusion recombinantes/biosynthèse , Protéines de fusion recombinantes/génétique , Protéines de fusion recombinantes/ultrastructure , Recombinaison génétique , Virion/ultrastructureRÉSUMÉ
Newcomb and coworkers (W. W. Newcomb, F. L. Homa, D. R. Thomsen, F. P. Booy, B. L. Trus, A. C. Steven, J. V. Spencer, and J. C. Brown, J. Mol. Biol. 263:432-446, 1996; W. W. Newcomb, F. L. Homa, D. R. Thomsen, Z. Ye, and J. C. Brown, J. Virol. 68:6059-6063, 1994) have recently described an in vitro herpes simplex virus (HSV) capsid assembly product which, because of certain parallels between its properties and those of bacteriophage proheads, they have designated the procapsid. As in their bacteriophage counterparts, there are marked differences between the structures of the two types of particle, and conversion from the procapsid to the capsid form requires extensive reconfiguration of the subunits. This reconfiguration occurs spontaneously upon extended in vitro incubation. One of the distinctive features of the HSV procapsids is that, unlike mature capsids, they are unstable and disassemble upon storage at 2 degrees C. Using a mutant of HSV type 1 (ts1201), which has a lesion in the protease responsible for maturational cleavage of the scaffolding protein, we have demonstrated that capsids present within cells infected at nonpermissive temperatures are also cryosensitive and disappear if the cells are incubated at 0 degrees C. This suggests that ts1201 capsids may resemble procapsids in structure. However, ts1201 capsids remain cryosensitive following extended incubation at an elevated temperature and, therefore, do not appear to undergo the spontaneous reconfiguration seen with in vitro-assembled procapsids. The lesion in ts1201 is reversible, and capsids formed at the nonpermissive temperature can undergo maturational cleavage and go on to form infectious virions following downshift to permissive temperatures. The sensitivity of ts1201 capsids to low temperatures is closely correlated with the cleavage status of the scaffolding protein, suggesting that proteolysis may act to trigger their conversion to the stable form. The experiments described here provide the firmest evidence yet that the procapsid has a biologically relevant role in the virus life cycle.
Sujet(s)
Capside/physiologie , Herpèsvirus humain de type 1/physiologie , Précurseurs de protéines/physiologie , Assemblage viral , Animaux , Capside/ultrastructure , Lignée cellulaire , Cricetinae , Herpèsvirus humain de type 1/génétique , Herpèsvirus humain de type 1/ultrastructure , Humains , Mutation , Serine endopeptidases/physiologie , Température , Protéines virales/physiologieRÉSUMÉ
The dielectric properties of baby hamster kidney fibroblast (BHK(C-13)) cells have been measured using electrorotation before and after infection with herpes simplex virus type 1 (HSV-1). The dielectric properties and morphology of the cells were investigated as a function of time after infection. The mean specific capacitance of the uninfected cells was 2.0 microF/cm2, reducing to a value of 1. 5 microF/cm2 at 12 h after infection. This change was interpreted as arising from changes in the cell membrane morphology coupled with alterations in the composition of the cell membrane as infection progressed. The measured changes in the cell capacitance were correlated with alterations in cellular morphology determined from scanning electron microscope (SEM) images. Between 9 and 12 h after infection the internal permittivity of the cell exhibited a rapid change, reducing in value from 75epsilono to 58epsilono, which can be correlated with the generation of large numbers of Golgi-derived membrane vesicles and enveloped viral capsids. The data are discussed in relation to the known life cycle of HSV-1 and indicate that electrorotation can be used to observe dynamic changes in both the dielectric and morphological properties of virus-infected cells. Calculations of the dielectrophoretic spectrum of uninfected and infected cells have been performed, and the results show that cells in the two states could be separated using appropriate frequencies and electrode arrays.
Sujet(s)
Herpèsvirus humain de type 1/pathogénicité , Rein/physiologie , Rein/virologie , Animaux , Phénomènes biophysiques , Biophysique , Lignée cellulaire , Membrane cellulaire/physiologie , Cricetinae , Conductivité électrique , Électrophysiologie , Fibroblastes/physiologie , Fibroblastes/ultrastructure , Fibroblastes/virologie , Herpèsvirus humain de type 1/croissance et développement , Rein/cytologie , Microscopie électronique à balayage , RotationRÉSUMÉ
Herpes simplex virus type 1 virions were examined by electron cryomicroscopy, allowing the three-dimensional structure of the infectious particle to be visualized for the first time. The capsid shell is identical to that of B-capsids purified from the host cell nucleus, with the exception of the penton channel, which is closed. The double-stranded DNA genome is organized as regularly spaced ( approximately 26 A) concentric layers inside the capsid. This pattern suggests a spool model for DNA packaging, similar to that for some bacteriophages. The bulk of the tegument is not icosahedrally ordered. However, a small portion appears as filamentous structures around the pentons, interacting extensively with the capsid. Their locations and interactions suggest possible roles for the tegument proteins in regulating DNA transport through the penton channel and binding to cellular transport proteins during viral infection.
Sujet(s)
Capside/ultrastructure , Herpèsvirus humain de type 1/ultrastructure , Protéines virales/ultrastructure , Virion/ultrastructure , Capside/métabolisme , Cryomicroscopie électronique , Herpèsvirus humain de type 1/génétique , Herpèsvirus humain de type 1/métabolisme , Humains , Traitement d'image par ordinateur , Protéines virales/métabolismeRÉSUMÉ
The frequency-dependent dielectrophoretic behaviour of an enveloped mammalian virus, herpes simplex virus type 1 is described. It is demonstrated that over the range 10 kHz-20 MHz, these viral particles, when suspended in an aqueous medium of conductivity 5 mS m(-1), can be manipulated by both positive and negative dielectrophoresis using microfabricated electrode arrays. The observed transition from positive to negative dielectrophoresis at frequencies around 4.5 MHz is in qualitative agreement with a simple model of the virus as a conducting particle surrounded by an insulating membrane.
Sujet(s)
Biophysique/méthodes , Herpèsvirus humain de type 1/composition chimique , Herpèsvirus humain de type 1/isolement et purification , Biophysique/instrumentation , Électrochimie , Électrophorèse/instrumentation , Électrophorèse/méthodes , Fluorescence , Herpèsvirus humain de type 1/ultrastructure , Humains , Microélectrodes , Photographie (méthode)RÉSUMÉ
Two proteins, VP19C (50,260 Da) and VP23 (34,268 Da), make up the triplexes which connect adjacent hexons and pentons in the herpes simplex virus type 1 capsid. VP23 was expressed in Escherichia coli and purified to homogeneity by Ni-agarose affinity chromatography. In vitro capsid assembly experiments demonstrated that the purified protein was functionally active. Its physical status was examined by differential scanning calorimetry, ultracentrifugation, size exclusion chromatography, circular dichroism, fluorescence spectroscopy, and 8-anilino-1-naphthalene sulfonate binding studies. These studies established that the bacterially expressed VP23 exhibits properties consistent with its being in a partially folded, molten globule state. We propose that the molten globule represents a functionally relevant intermediate which is necessary to allow VP23 to undergo interaction with VP19C in the process of capsid assembly.
Sujet(s)
Protéines de capside , Capside/composition chimique , Herpèsvirus humain de type 1/composition chimique , Séquence nucléotidique , Capside/génétique , Amorces ADN/génétique , Escherichia coli/génétique , Herpèsvirus humain de type 1/génétique , Plasmides/génétique , Conformation des protéines , Pliage des protéines , Structure tertiaire des protéines , Protéines de fusion recombinantes/composition chimique , Protéines de fusion recombinantes/génétiqueRÉSUMÉ
Formation of herpes simplex virus-1 capsids requires the presence of intact scaffolding proteins. The C terminus of the abundant scaffolding protein associates with the major capsid shell protein VP5 through hydrophobic interactions. After cleavage by the viral encoded protease, which removes their C-terminal 25 aa, the scaffolding proteins are released from the capsid. We have used electron cryomicroscopy and computer image processing to determine, to 13 A, the three-dimensional structures of capsids containing either cleaved or uncleaved scaffolding proteins. Detailed comparisons show that the structures of the outer icosahedral shells are almost identical in the two capsid types. Differences are apparent in the radial distribution of the density inside the capsid shell (within a radius of 460 ) which represents the scaffolding core. However, in both capsid types, the bulk of this internal density exhibits no icosahedral symmetry. Close examination revealed localized regions of icosahedrally arranged extra density at the interface between the outer shell and the scaffold of protease-minus capsids. Rod-like densities extending inwards for approximately 40 from the capsid shell are present under four of the six quasi-equivalent triplex positions. Under triplexes Tb, Tc, and Te, the major additional densities appear as pairs with the rods in each pair situated 37 apart. We propose that these rods are formed by the C-termini of the scaffolding proteins and represent the sites of interaction between the capsid shell and scaffold.
Sujet(s)
Capside/ultrastructure , Herpèsvirus humain de type 1/ultrastructure , Protéines virales , Capside/déficit , Cryoconservation , Herpèsvirus humain de type 1/croissance et développement , Traitement d'image par ordinateur/méthodes , Microscopie électronique , Maquettes de structure , Serine endopeptidases/déficitRÉSUMÉ
Electron cryomicroscopy and icosahedral reconstruction are used to obtain the three-dimensional structure of the 1250-A-diameter herpesvirus B-capsid. The centers and orientations of particles in focal pairs of 400-kV, spot-scan micrographs are determined and iteratively refined by common-lines-based local and global refinement procedures. We describe the rationale behind choosing shared-memory multiprocessor computers for executing the global refinement, which is the most computationally intensive step in the reconstruction procedure. This refinement has been implemented on three different shared-memory supercomputers. The speedup and efficiency are evaluated by using test data sets with different numbers of particles and processors. Using this parallel refinement program, we refine the herpesvirus B-capsid from 355-particle images to 13-A resolution. The map shows new structural features and interactions of the protein subunits in the three distinct morphological units: penton, hexon, and triplex of this T = 16 icosahedral particle.
Sujet(s)
Capside/composition chimique , Capside/ultrastructure , Conformation des protéines , Simplexvirus/composition chimique , Simulation numérique , Congélation , Microscopie électronique , Modèles moléculaires , Simplexvirus/ultrastructureRÉSUMÉ
The scaffolding protein and associated protease of the human herpesvirus varicella-zoster virus (VZV), encoded by genes 33.5 and 33 respectively, were synthesized in insect cells using a baculovirus expression system. The expressed 33.5 product formed numerous long, flexible, hollow rods, and in this respect different from the herpes simplex virus type 1 (HSV-1) homologue which forms large aggregates consisting mainly of fibrous material interspersed with scaffold-like particles. Removal of 27 amino acids from the carboxy terminus of the VZV scaffolding protein by the gene 33 protease or expression of the cleaved product did not result in any discernible change in the morphology of the scaffolding protein. Again, this was in marked contrast to the situation in HSV-1 where removal of the 25 carboxy-terminal amino acids from the scaffolding protein by the associated protease or expression of VP22a results in the formation of large numbers of scaffold-like particles. Despite these differences, when cells were multiply infected with baculoviruses expressing the HSV-1 capsid shell proteins and the VZV scaffolding protein complete capsids were observed, suggesting that the VZV protein could act as a scaffold for the assembly of the HSV-1 capsid shell. The efficiency of capsid assembly was increased substantially by exchanging the 23 carboxy-terminal amino acids of the VZV scaffolding protein for the corresponding 22 carboxy-terminal amino acids of the HSV-1 homologue, supporting previous work which showed that this region was critical for the formation of intact capsids.
Sujet(s)
Capside/physiologie , Herpèsvirus humain de type 1/physiologie , Herpèsvirus humain de type 3/métabolisme , Serine endopeptidases/métabolisme , Protéines virales/métabolisme , Séquence d'acides aminés , Animaux , Capside/métabolisme , Protéines de capside , Lignée cellulaire , Cricetinae , Vecteurs génétiques , Herpèsvirus humain de type 3/ultrastructure , Humains , Souris , Données de séquences moléculaires , Nucleopolyhedrovirus , Structure secondaire des protéines , Protéines de fusion recombinantes/génétique , Protéines de fusion recombinantes/métabolisme , Serine endopeptidases/génétique , Protéines virales/génétique , Assemblage viralRÉSUMÉ
Herpes simplex virus type 1 (HSV-1) capsid assembly takes place in the nucleus of infected cells. However, when each of the outer capsid shell proteins, VP5, VP23 and VP26, is expressed in the absence of any other HSV-1 proteins, it does not localize to the nucleus but is distributed throughout the cell. We have previously shown that the HSV-1 capsid scaffolding protein, preVP22a, can relocate VP5 into the nucleus but does not influence the distribution of VP23. We now demonstrate that the outer capsid shell protein, VP19C, is able to relocate both VP5 and VP23 separately into the nucleus. However, nuclear localization of VP26 is only observed when VP5 is present together with either VP19C or preVP22a. Thus, pair-wise interactions involving all of the abundant capsid proteins have now been identified. Electron microscope examination of insect cells coinfected with recombinant baculoviruses expressing VP19C and VP5 reveals the presence of 70 nm diameter 'capsid-like' structures, suggesting that these two proteins can form the basic capsid shell.
Sujet(s)
Capside/métabolisme , Herpèsvirus humain de type 1/métabolisme , Séquence d'acides aminés , Animaux , Baculoviridae/génétique , Baculoviridae/ultrastructure , Séquence nucléotidique , Transport biologique , Capside/génétique , Protéines de capside , Lignée cellulaire , Cricetinae , ADN viral , Herpèsvirus humain de type 1/génétique , Humains , Souris , Données de séquences moléculaires , Protéines de fusion recombinantes/génétique , Protéines de fusion recombinantes/métabolisme , Spodoptera/cytologieRÉSUMÉ
The tegument of herpes simplex virus type 1 (HSV-1) virus particles is a complex assemblage of virus proteins whose relative proportions within virions are essentially constant for a particular strain of virus. To examine the processes controlling incorporation into the tegument, we constructed a HSV-1 recombinant that expresses two copies of gene UL49, which encodes the major tegument protein VP22. One copy specifies the unmodified form of VP22 under the control of the native promoter while the second expresses an epitope-tagged version of the protein via the human cytomegalovirus immediate early promoter. In cells infected with the recombinant virus, the overall levels of VP22 synthesized were about fivefold higher than those for wild-type virus, due to the high levels of expression of tagged protein. Analysis of virus particles revealed that the amount of VP22 in the tegument was approximately two- to threefold higher in recombinant virions and L-particles than in particles produced by wild-type virus. These results provide the first evidence that, for certain proteins, the level of polypeptide synthesis can act as a controlling factor for the amount of protein incorporated into tegument.
Sujet(s)
Herpèsvirus humain de type 1/métabolisme , Protéines virales structurales/métabolisme , Séquence d'acides aminés , Animaux , Séquence nucléotidique , Lignée cellulaire , Centrifugation en gradient de densité , Cricetinae , ADN viral , Épitopes , Ficoll , Herpèsvirus humain de type 1/génétique , Herpèsvirus humain de type 1/physiologie , Humains , Données de séquences moléculaires , Protéines recombinantes/génétique , Protéines de l'enveloppe virale/métabolisme , Protéines virales structurales/génétique , Protéines virales structurales/immunologie , Assemblage viralRÉSUMÉ
Previous studies have shown that the protein encoded by herpes simplex virus type 1 (HSV-1) gene UL6 is required for processing and packaging of replicated viral DNA and is a minor component of virions and capsids. In this report, we describe the construction of UL6- HSV-1 mutants with a disrupted UL6 gene using complementing cells and show that they fail to synthesize the UL6 protein or produce infectious virus in noncomplementing cells. The mutants synthesized but failed to process and encapsidate viral DNA and accumulated only immature capsids which lacked the UL6 protein. Immunofluorescence analysis showed that the UL6 protein, when expressed transiently in transfected cells in the absence of other HSV-1 proteins, is localized exclusively to the nucleus. We also investigated an HSV-1 mutant with a defect in gene UL33, the product of which is also thought to be involved in viral DNA processing and packaging. The phenotype of this mutant on noncomplementing cells with regard to failure to process and encapsidate viral DNA, accumulation of immature capsids, and inability to produce infectious virus was the same as that of UL6- viruses. This mutant, however, produced capsids containing the UL6 protein, indicating that association of the UL6 protein with the capsid is independent of the UL33 protein.
Sujet(s)
Capside/physiologie , Herpèsvirus humain de type 1/physiologie , Animaux , Capside/génétique , Capside/immunologie , Capside/isolement et purification , Capside/ultrastructure , Lignée cellulaire , Noyau de la cellule/virologie , Chlorocebus aethiops , Cricetinae , ADN viral/génétique , Virus défectifs/génétique , Virus défectifs/isolement et purification , Virus défectifs/physiologie , Virus défectifs/ultrastructure , Herpèsvirus humain de type 1/génétique , Herpèsvirus humain de type 1/isolement et purification , Herpèsvirus humain de type 1/ultrastructure , Humains , Microscopie électronique , Mutagenèse par insertion , Protéines recombinantes , Cellules Vero , Protéines virales/génétique , Réplication virale/génétique , Réplication virale/physiologieRÉSUMÉ
The 1250 A diameter herpes simplex virus-1 (HSV-1) capsid shell consists of four major structural proteins, of which VP26 (approximately 12,000 M(r)) is the smallest. Using 400 kV electron cryomicroscopy and computer reconstruction, we have determined the three-dimensional structures of the wild-type capsid and a recombinant baculovirus-generated HSV-1 capsid which lacks VP26. Their difference map demonstrates the presence of VP26 hexamers attached to all the hexons in the wild-type capsid, and reveals that the VP26 molecule consists of a large and a small domain. Although both hexons and pentons are predominantly composed of VP5, VP26 is not present on the penton. Based on the interactions involving VP26 and the hexon subunits, we propose a mechanism for VP26 assembly which would account for its distribution. Possible roles of VP26 in capsid stability and DNA packaging are discussed.