RESUMO
Objective:To obtain the optimum extraction conditions for quercetin and kaempferol from Lindera aggregate leaves. Methods:On the basis of single factor investigation, Box-Behnken experimental design was used to provide the experimental data for establishing a regression model for the extraction of quercetin and kaempferol. Response surface and contour diagrams with the extrac-tion yield of quercetin and kaempferol as the response values,were plotted for analyzing the pairwise interactive effects of hydrochloric acid concentration, hydrolysis temperature and hydrolysis time. Results:The hydrochloric acid concentration of 4. 1%, the hydrolysis temperature of 83℃ and the hydrolysis time of 45min were the optimum extraction conditions. With the above conditions, the content of quercetin and kaempferol from Lindera aggregate leaves was 6. 97 mg·g-1 and 2. 82 mg·g-1 , respectively. The Lindera aggregate beaves from five different habitals were analyzed,and the total content of quercetin and kaempferide from Taizhou Tiantai were highest. Conclusion:The quercetin and kaempferol extraction from Lindera aggregate leaves is optimized by Box-Behnken response surface method and the process is convenient and feasible.
RESUMO
Objective:To obtain the optimum extraction conditions for quercetin and kaempferol from Lindera aggregate leaves. Methods:On the basis of single factor investigation, Box-Behnken experimental design was used to provide the experimental data for establishing a regression model for the extraction of quercetin and kaempferol. Response surface and contour diagrams with the extrac-tion yield of quercetin and kaempferol as the response values,were plotted for analyzing the pairwise interactive effects of hydrochloric acid concentration, hydrolysis temperature and hydrolysis time. Results:The hydrochloric acid concentration of 4. 1%, the hydrolysis temperature of 83℃ and the hydrolysis time of 45min were the optimum extraction conditions. With the above conditions, the content of quercetin and kaempferol from Lindera aggregate leaves was 6. 97 mg·g-1 and 2. 82 mg·g-1 , respectively. The Lindera aggregate beaves from five different habitals were analyzed,and the total content of quercetin and kaempferide from Taizhou Tiantai were highest. Conclusion:The quercetin and kaempferol extraction from Lindera aggregate leaves is optimized by Box-Behnken response surface method and the process is convenient and feasible.
