RESUMO
BACKGROUND: Currently, gastric cancer (GC) is considered a public health problem worldwide. Using medicinal plants for the prevention of chronic diseases such as cancer constitutes new alternatives in traditional medicine. Oenothera rosea (OR) could be an option, but it needs to be evaluated. AIM: The main objective of this study was to evaluate the protective effect of OR extract on N-methyl-N-nitrosourea (NMU)-induced GC in rats. METHODS: In total, 80 male Holtzman rats were randomized into five groups. Group A received the saline solution (5mL/kg), group B received NMU 500 µg/kg (cancer inductor) by oral administration for 16 weeks, and groups C, D, and E were treated with OR extract (100, 200, and 300 mg/kg, respectively) and NMU in order to evaluate the preventive effect on cancer induced by NMU for 16 weeks. Blood and histological samples of stomachs were collected to determine histopathological, biochemical, and hematological parameters between different experimental groups. RESULTS: Groups C, D, and E presented less histopathological changes such as anaplastic and hyperplastic cells, compared with group B. Hematological and biochemical parameters were recorded, and superoxide dismutase, malondialdehyde, and nitric oxide levels were statistically less than those of NMU group (P<0.05, P<0.01, and P<0.01). CONCLUSION: Considering the histopathological signs and the antioxidant activity in vivo as well as hematological and biochemical parameters of ethanolic extract of OR, we concluded that its administration in rats has a protective effect on GC, which is induced experimentally. This species could be studied in clinical trials for patients with GC in the future.
RESUMO
La investigación tuvo como objetivo evaluar la eficacia y seguridad de supositorios de aceite de Copaifera officinalis L. "copaiba" en pacientes con diágnóstico definitivo de crisis hemorroidal aguda. El ensayo fue clínico prospectivo, controlado, comparativo, aleatorio y se realizó en el Instituto de Investigaciones Clínicas de la Facultad de Medicina de UNMSM. y Servicio de Gastroenterología del Hospital Nacional Dos de Mayo. Los pacientes evaluados presentaban diagnóstico definitivo de crisis hemorroidal aguda. El diagnóstico se realizó mediante estudio clínico y proctoscópico antes y después del tratamiento, además de exámenes auxiliares serológicos; 60 pacientes fueron enrolados voluntariamente al protocolo cumpliendo con los criterios éticos de investigación: los cuales fueron distribuidos en 3 grupos de 20 casos cada uno, administrándoseles supositorios de copaiba 120 mg bid, copaiba 120 mg/lidocaína 30 mg bid, y Prednisolona caproato 1,3 mg/Cincocaína clorhídrato 1 mg respectivamente durante 7 días. Los datos obtenidos como el alivio de la sintomatología, disminución del tamaño de las hemorroides y presencia de eventos adversos fueron evaluados mediante técnicas multivariadas (p<0,05). Se logró mejoría clínica y desaparición de síntomas de la enfermedad, sin efectos adversos significativos; dos presentaron dolor y ardor anal de manera transitoria, y no requirió suspensión de la medicación. Se concluyó que los supositorios de aceita de Copaifera officinalis es una alternativa eficaz y segura en el tratamiento de la crisis hemorroidal aguda.
The present study aimed to evaluate the efficacy and safety of oil suppositories Copaifera officinalis L. "copaiba" in patients with a final diagnosis of acute hemorrhoidal crisis. The trial was prospective, controlled, comparative, randomized and performed in the Clinical Research Institute of the Faculty of Medicine UNMSM Gastroenterology and National Hospital Dos de Mayo. Patients evaluated had definite diagnosis of acute hemorrhoidal crisis. The diagnosis was made by clinical and proctoscópico before and after treatment, plus auxiliary serologic tests, 60 patients were enrolled voluntarily complying with the protocol ethical research, which were divided into 3 groups of 20 cases each, was administered suppositories 120 mg bid copaiba 120 mg/lidocaine 30mg bid, and prednisole caproate 13 mg/Cinchocaine hydrochloride 1 mg respectively for 7 days. Data obtained as relief of symptoms, decrease the size of hemorrhoids and presence of adverse events were assessed by multivariate techniques (p<0.05). was achieved clinical improvement and disappearance of symptoms of the symptoms of the disease, without significant adverse effects, two had anal burning pain temporaly, and did not require drug discontinuation. It was concluded that oil suppositories Copaifera officinalis is an effective and safe alternative in the treatment of acute hemorrhoidal crisis.
Assuntos
Fabaceae , Hemorroidas , Supositórios , Óleos de PlantasRESUMO
Objetivos: Determinar la eficacia cicatrizante del aceite de copaiba obtenido de la corteza de Copaifera officinalis, comparado con omeprazol 20 mg, en pacientes con diagnóstico definitivo de úlcera péptica. Diseño: Estudio experimental, clínico comparativo, de fase II, aleatorio, doble ciego, grupo paralelo. Institución: Instituto de Investigaciones Clínicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Participantes: Pacientes con diagnóstico definitivo de úlcera péptica. Intervenciones: El diagnóstico fue tanto por exploración física como complementaria, siendo la endoscopia la técnica de elección, con evaluación pre y postratamiento con aceite de copaiba, formulada en cápsulas de 80 mg y 120 mg. El ensayo clínico incluyó 60 pacientes que voluntariamente ingresaron al programa de estudio, previa firma del consentimiento informado aprobado por el Comité institucional de Ética en Investigación. Los pacientes fueron distribuidos aleatoriamente en tres grupos, de 20 casos cada uno, según orden de llegada; los dos primeros grupos recibieron cápsulas de aceite de copaiba, en dosis de 80 y 120 mg, respectivamente; y un tercer grupo recibió omeprazol 20 mg. Los tratamientos fueron administrados en ayunas, una vez por la mañana, 30 minutos antes de la ingesta del primer alimento. Los datos fueron evaluados mediante técnicas multivariadas, considerando estadísticamente significativo p<0,05. Se tuvo en cuenta el consentimiento informado aprobado por el Comité de Bioética en Investigación del Centro Asistencial. Principales medidas de resultados: Porcentaje de cicatrización. Resultados: Se logró 65 por ciento y 75 por ciento de cicatrización de la úlcera péptica con aceite de copaiba, respectivamente, contra 100 por ciento en el grupo de omeprazol, sin efectos adversos significativos; dos presentaron náuseas y tres epigastralgia. Conclusiones: Los pacientes con úlcera péptica y con tratamiento de las cápsulas conteniendo aceite de copaiba mostraron cicatrización de la úlcera de 65 a 75 por ciento y sin efectos adversos significativos.
Objectives: To determine the cicatrizing effect of Copaifera officinalisÆ stem bark copaiba oil compared with omeprazole 20 mg in patients with diagnosis of peptic ulcer. Design: Experimental, comparative, phase II, randomized, double-blind, parallel-group clinical trial. Setting: Clinical Investigation Institute, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Participants: Patients with diagnosis of peptic ulcer. Interventions: Clinical diagnosis of peptic ulcer was done by endoscopy as well as pre and post treatment evaluation following administration of copaiba oil formulated capsules (80 mg and 120 mg). Sixty patients enrolled voluntarily to the study and signed informed consent as approved by the Institutional Review Board. Patients were randomly distributed in three groups of 20 cases each according to arrival order; the first and second group received respectively copaiba oil 80 mg and 120 mg capsules, and the third group omeprazole 20 mg, fasting, once daily in the morning, half hour before breakfast. Data was evaluated through multivariate techniques, considering p<0.05 as statistically significant. Main outcome measures: Percentage of patients healing their ulcer. Results: Peptic ulcer cicatrized in 65 per cent and 75 per cent respectively versus 100 per cent in the omeprazole group, without significant adverse effects. Two patients presented nausea and three epigastric pain. Conclusions: Patients with peptic ulcer treated with copaiba oil capsules showed ulcer scarring in 65 per cent to 75 per cent and without significant adverse effects.
