Water resources and nitrate discharges in relation to agricultural land uses in an intensively irrigated watershed.

Application of integrated hydrological models to manage water resources and non-point agricultural pollutants are increasingly used in decision-making processes. In this study SWAT (Soil and Water Assessment Tool) was used to simulate the water balance and nitrate pollution in an intensively irrigated agricultural catchment (Flumen River in Monegros, Aragon, NE Spain). Rainfall comprised only 45% of the inputs of water in the Flumen watershed and the rest is contributed through irrigation canals from two other rivers outside the Flumen watershed. Green water storage and green water flow are the dominant components of the water balance in the watershed, which is related to the important contribution of water for irrigation. In general, green water storage and green water flow are quite similar in the subwatersheds dominated by irrigation agriculture that are located in the central part of the watershed. A similar pattern was observed for blue water, with high amounts in the central irrigated subwatersheds compared to the non-irrigated subwatersheds. Consequently, nitrate infiltration in the aquifer was higher in the inner irrigated subwatersheds (100-250 kg N ha-1 year-1) but much lower than the lateral flow rates estimated in the non-irrigated subwatersheds (1400-2000 kg N ha-1 year-1). Two scenarios simulating the effects of expected climate change factors in this zone were performed. A reduction in the availability of water for irrigation will transform the area from irrigated crops to cereal. In this case the water flow of River Flumen at the outlet of the watershed is reduced by 15%. If a reduction of 40% nitrate fertilization is applied, the nitrate exported to Flumen River would decreased by 28%. These results suggest that dosing irrigation water and fertilizers in accordance with crop requirements would contribute to buffer peaks of water and nitrate discharges and to a more efficient agricultural use of the resources.

A Methodological Approach for Spatiotemporally Analyzing Water-Polluting Effluents in Agricultural Landscapes Using Partial Triadic Analysis.

Multivariate techniques for two-dimensional data matrices are normally used in water quality studies. However, if the temporal dimension is included in the analysis, other statistical techniques are recommended. In this study, partial triadic analysis was used to investigate the spatial and temporal variability in water quality variables sampled in a northeastern Spain river basin. The results highlight the spatiality of the physical and chemical properties of water at different sites along a river over 1 yr. Partial triadic analysis allowed us to clearly identify the presence of a stable spatial structure that was common to all sampling dates across the entire catchment. Variables such as electrical conductivity and Na and Cl ions were associated with agricultural sources, whereas total dissolved nitrogen, NH-N concentrations, and NO-N concentrations were linked to polluted urban sites; differences were observed between irrigated and nonirrigated periods. The concentration of NO-N was associated with both agricultural and urban land uses. Variables associated with urban and agricultural pollution sources were highly influenced by the seasonality of different activities conducted in the study area. In analyzing the impact of land use and fertilization management on water runoff and effluents, powerful statistical tools that can properly identify the causes of pollution in watersheds are important. Partial triadic analysis can efficiently summarize site-specific water chemistry patterns in an applied setting for land- and water-monitoring schemes at the landscape level. The method is recommended for land-use decision-making processes to reduce harmful environmental effects and promote sustainable watershed management.

Integrating objectives and scales for planning and implementing wetland restoration and creation in agricultural landscapes.

Traditionally, wetland management strategies have focused on single familiar objectives, such as improving water quality, strengthening biodiversity, and providing flood control. Despite the relevant amount of studies focused on wetland creation or restoration with these and other objectives, still little is known on how to integrate objectives of wetland creation or restoration at different landscape scales. We have reviewed the literature to this aim, and based on the existing current knowledge, we propose a four step approach to take decisions in wetland creation or restoration planning. First, based on local needs and limitations we should elucidate what the wetland is needed for. Second, the scale at which wetland should be created or restored must be defined. Third, conflicts and compatibilities between creation or restoration objectives must then be carefully studied. Fourth, a creation or restoration strategy must be defined. The strategy can be either creating different unipurpose wetlands or multipurpose wetlands, or combinations of them at different landscape scales. In any case, in unipurpose wetland projects we recommend to pursue additional secondary objectives. Following these guidelines, restored and created wetlands would have more ecological functions, similar to natural wetlands, especially if spatial distribution in the landscape is considered. Restored and created wetlands could then provide an array of integrated environmental services adapted to local ecological and social needs.

Viscous cavity damping of a microlever in a simple fluid.

We consider the problem of oscillation damping in air of a thermally actuated microlever as it gradually approaches an infinite wall in parallel geometry. As the gap is decreased from 20 microm down to 400 nm, we observe the increasing damping of the lever Brownian motion in the fluid laminar regime. This manifests itself as a linear decrease in the lever quality factor accompanied by a dramatic softening of its resonance, and eventually leads to the freezing of the CL oscillation. We are able to quantitatively explain this behavior by analytically solving the Navier-Stokes equation with perfect slip boundary conditions. Our findings may have implications for microfluidics and micro- and nanoelectromechanical applications.

A MEMS-based high frequency x-ray chopper.

Time-resolved x-ray experiments require intensity modulation at high frequencies (advanced rotating choppers have nowadays reached the kHz range). We here demonstrate that a silicon microlever oscillating at 13 kHz with nanometric amplitude can be used as a high frequency x-ray chopper. We claim that using micro-and nanoelectromechanical systems (MEMS and NEMS), it will be possible to achieve higher frequencies in excess of hundreds of megahertz. Working at such a frequency can open a wealth of possibilities in chemistry, biology and physics time-resolved experiments.

Mechanical mode dependence of bolometric backaction in an atomic force microscopy microlever.

Two backaction (BA) processes generated by an optical cavity-based detection device can deeply transform the dynamical behavior of an atomic force microscopy microlever: the photothermal force or the radiation pressure. Whereas noise damping or amplifying depends on the optical cavity response for radiation pressure BA, we present experimental results carried out under vacuum and at room temperature on the photothermal BA process which appears to be more complex. We show for the first time that it can simultaneously act on two vibration modes in opposite directions: Noise on one mode is amplified, whereas it is damped on another mode. Basic modeling of photothermal BA shows that the dynamical effect on the mechanical mode is laser spot position-dependent with respect to mode shape. This analysis accounts for opposite behaviors of different modes as observed.

Aging and immune response in chronic human schistosomiasis.

There has been no systematic study of the immune response of individuals over 60 residing in areas endemic for Schistosoma mansoni infection although senescence is reportedly associated with susceptibility to infection and progressive decline in immune function. We have shown previously, in two endemic areas in Minas Gerais, Brazil, that the frequency of individuals over 60 with chronic schistosomiasis is no longer negligible. Several aging-related immunological alterations are already described in medical literature, mostly in the T-cell compartment. Since aging is associated with a decline in T-cell function, it is not surprising that individuals over 60 would be more susceptible to infection. However, not all aged individuals in endemic areas have high intensity of infection; some of them display a negative stool-screening test for the presence of schistosome eggs and low levels of serum antibodies reactive with S. mansoni antigens indicating they are not infected. Non-infected, negative individuals may develop compensatory mechanisms to cope with immune dysfunction and to generate protective responses against the constant threat of infection in these areas. Herein, we reviewed previous reports from our group showing that two mechanisms contribute to distinguish between infected and egg negative aged individuals. First, egg negative aged individuals develop innate immune responses to replace the decline in T-cell function that is observed with aging. Second, chronically activated regulatory T cells, that may impair protective immune responses, are more vigorous in infected aged individuals. We propose that egg negative individuals may be considered as an example of healthy aging in areas endemic for infectious disease.

X-ray pushing of a mechanical microswing.

We report here for the first time the combination of x-ray synchrotron light and a micro-electro-mechanical system (MEMS). We show how it is possible to modulate in real time a MEMS mass distribution to induce a nanometric and tunable mechanical oscillation. The quantitative experimental demonstration we present here uses periodic thermal dilatation of a Ge microcrystal attached to a Si microlever, induced by controlled absorption of an intensity modulated x-ray microbeam. The mechanism proposed can be envisaged either for the detection of small heat flux or for the actuation of a mechanical system.

Ageing and Toll-like receptor expression by innate immune cells in chronic human schistosomiasis.

There has been no systematic study of the immune response of individuals aged over 60 years living in Schistosomiasis mansoni-endemic areas, although senescence is reportedly associated with susceptibility to infection and progressive decline in immune function. We have shown previously, in two endemic areas in Minas Gerais, Brazil, that the frequency of individuals aged over 60 years with chronic schistosomiasis is no longer negligible. Moreover, several elderly individuals who have always lived in these endemic areas stay protected from infection. An important question for studies of ageing and disease control in developing countries is which differences in the immunological profile of these negatively tested (non-infected) individuals can account for their resistance to either infection or reinfection. We show, in the present study, that non-infected (negative) elderly individuals develop innate immune mechanisms of protection that replace the age-associated decline in T cell function. Non-infected elderly individuals from endemic areas of schistosome infection present an increase in the frequency of the natural killer (NK) CD56(low) subset of NK cells expressing Toll-like receptors (TLR)-1, -2, -3 and -4 as determined by flow cytometry analysis. In addition, the proportion of dendritic cells expressing TLR-1 is elevated as well as the frequency of monocytes expressing TLR-1 and -4. These results suggest that TLR expression by cells of the innate immune system may be related to the negative status of infection in some elderly individuals who are constantly exposed to S. mansoni. Developing mechanisms of protection from infection may represent a biomarker for healthy ageing in this population.

Antibody response in cattle after vaccination with inactivated and attenuated rabies vaccines.

Despite the absence of current official reports showing the number of cattle infected by rabies, it is estimated that nearly 30,000 bovines are lost each year in Brazil. In order to minimize the important economic losses, control of the disease is achieved by eliminating bat colonies and by herd vaccination. In this study, we compare the antibody response in cattle elicited by vaccination with an attenuated ERA vaccine (AEvac) and an inactivated-adjuvanted PV (IPVvac) vaccine. The antibody titers were appraised by cell-culture neutralization test and ELISA, and the percentage of seropositivity was ascertained for a period of 180 days. IPVvac elicited complete seropositivity rates from day 30 to day 150, and even on day 180, 87% of the sera showed virus-neutralizing antibody titers (VNA) higher than 0.5IU/ml. There were no significant differences between the VNA titers and seropositivity rates obtained with IPVvac in the two methods tested. AEvac, however, elicited significantly lower titers than those observed in the group receiving inactivated vaccine. In addition, the profiles of antirabies IgG antibodies, evaluated by ELISA, and VNA, appraised by cell-culture neutralization test, were slightly different, when both vaccines were compared.

Trace element analysis on Si wafer surfaces by TXRF at the ID32 ESRF undulator beamline.

Synchrotron radiation total-reflection X-ray fluorescence (SR-TXRF) has been applied to the impurity analysis of Si wafers using a third-generation synchrotron radiation undulator source. A lower limit of detectability (LLD) for Ni atoms of 17 fg (1.7 x 10(8) atoms cm(-2)) has been achieved with an optical set-up based on an Si(111) double-crystal monochromator and a horizontal sample geometry. These first results are very promising for synchrotron radiation trace element analysis since we estimate that it is possible to lower the LLD by a factor of about 25 by employing appropriate optics and detectors. The use of a crystal monochromator opens new possibilities to perform absorption and scattering experiments (NEXAFS and X-ray standing-wave methods) for chemical and structural analysis of ultratrace elements.

X-Ray Interferometry at ESRF Using Two Coherent Beams from Fresnel Mirrors.

Using two small flat mirrors under grazing incidence, we have produced interference patterns from partially coherent x-ray beams at the European Synchrotron Radiation Facility. By piezoelectrically orienting one mirror around the horizontal plane, both the vertical and horizontal transverse coherence distances of the radiation have been measured. The experimental setup can be used to characterize the coherence properties along x-ray synchrotron beamlines.