Collapse of scallop Nodipecten nodosus production in the tropical Southeast Brazil as a possible consequence of global warming and water pollution.

Mollusc rearing is a relevant global socioeconomic activity. However, this activity has faced severe problems in the last years in southeast Brazil. The mariculture scallop production dropped from 51,2 tons in 2016 to 10,2 tons in 2022 in the Baia da Ilha Grande (BIG; Rio de Janeiro). However, the possible causes of this collapse are unknown. This study aimed to analyze decadal trends of water quality in Nodipecten nodosus spat and adult production in BIG. We also performed physical-chemical and biological water quality analyses of three scallop farms and two nearby locations at BIG in 2022 to evaluate possible environmental stressors and risks. Scallop spat production dropped drastically in the last five years (2018-2022: mean ± stdev: 0.47 ± 0.45 million). Spat production was higher in colder waters and during peaks of Chlorophyll a in the last 13 years. Reduction of Chlorophyll a coincided with decreasing spat production in the last five years. Warmer periods (>27 °C) of the year may hamper scallop development. Counts of potentially pathogenic bacteria (Vibrios) and Escherichia coli were significantly higher in warmer periods which may further reduce scallop productivity. Shotgun metagenomics of seawater samples from the five studied corroborated these culture-based counts. Vibrios and fecal indicator bacteria metagenomic sequences were abundant across the entire study area throughout 2022. The results of this study suggest the collapse of scallop mariculture is the result of a synergistic negative effect of global warming and poor seawater quality.

Brumadinho dam collapse induces changes in the microbiome and the antibiotic resistance of the Paraopeba River (Minas Gerais, Brazil).

The rupture of the Córrego do Feijão dam in Brumadinho (January 25, 2019) caused serious damage to the Paraopeba River and compromised the quality of its waters for human consumption. However, the possible effects of the dam collapse on the river microbiome and its antibiotic resistance profiles are unknown. The present study aims to analyse the possible shifts in microbial diversity and enhancement of antibiotic resistance in the Paraopeba River. To this end, two sampling campaigns (February and May 2019) were performed to obtain water across the entire Paraopeba River (eight sampling locations: Moeda, Brumadinho, Igarapé, Juatuba, Varginha, Angueretá, Retiro Baixo and Três Marias; ~464 km). This sampling scheme enabled determining the effects of the disaster on the river microbiome. Total DNA and microbial isolation were performed with these water samples. The 16S rRNA-based microbiome analyses (n = 24; 2.05 million 16S rRNA reads) showed changes in microbial diversity immediately after the disaster with the presence of metal-indicating bacteria (Acinetobacter, Bacillus, Novosphingobium, and Sediminibacterium). Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) identification of bacterial isolates (n = 170) also disclosed possible indicators of faecal contamination across the Paraopeba (Cloacibacterium, Bacteroides, Feaecalibacterium, Bifidobacterium, Citrobacter, Enterobacter, Enterococcus and Escherichia). Antibiotic resistance increased significantly to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanate, ceftriaxone, and cefalotin among isolates obtained in May after the disaster. The effects of toxic mud on microbiomes were felt at all points sampled up to Anguereta. The ore mud may have exacerbated the growth of different antibiotic-resistant, metal-resistant, and faecal-indicating bacteria in the Paraopeba River.

Fluxes of the Amazon River plume nutrients and microbes into marine sponges.

Sponges have co-evolved with microbes for over 400 myr. Previous studies have demonstrated that sponges can be classified according to the abundance of microbes in their tissues as Low Microbial Abundance (LMA) and High Microbial Abundance (HMA). While LMA sponges rely mainly on water column microbes, HMA appear to rely much more on symbiotic fermentative and autotrophic microbes maintained in their tissues. However, it is unclear if this pattern holds when comparing different species of tropical sponges under extreme nutrient conditions and sediment loads in the water column, such as the Great Amazon Reef System (GARS), which covers an area of ~56,000 km2 off the Amazon River mouth. Sponges are the major GARS benthic components. However, these sponges' microbiome across the GARS is still unknown. Here, we investigated water quality, isotopic values (δ13C and δ15N), metagenomic and lipidomic profiles of sponges obtained from different sectors throughout the GARS. >180 million shotgun metagenomic reads were annotated, covering 22 sponge species. Isotopic and lipidomic analyses suggested LMA sponges rely on the Amazon River Plume for nutrition. HMA sponges (N = 15) had higher Roseiflexus and Nitrospira abundance, whereas LMA sponges (N = 7) had higher Prochlorococcus and Pelagibacter abundance. Functional data revealed that the LMA sponge microbiomes had greater number of sequences related to phages and prophages as well as electron transport and photophosphorylation which may be related to photosynthetic processes associated with the Prochlorococcus and Synechococcus found in the LMA. The higher phages abundance in LMA sponges could be related to these holobionts' reduced defense towards phage infection. Meanwhile, HMA sponge microbiomes had higher Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR abundance, which may be involved in defense against phage infection. This study sheds light on the nutrient fluxes and microbes from the Amazon River plume into the sponge holobionts.

Risk of Collapse in Water Quality in the Guandu River (Rio de Janeiro, Brazil).

The Guandu River, one of the main rivers in the state of Rio de Janeiro, provides water for more than nine million people in the metropolitan region. However, the Guandu has suffered from massive domestic and industrial pollution for more than two decades, leading to high levels of dissolved total phosphorus, cyanobacteria, and enteric bacteria observed during the summers of 2020 and 2021. The use of Phoslock, a palliative compound, was not effective in mitigating the levels of phosphorus in the Guandu River. Furthermore, potable water driven from the river had levels of 2-MIB/geosmin and a mud smell/taste. With all these problems, several solutions are proposed for improving the Guandu River water quality, including establishment of (i) sewage treatment plants (STPs), (ii) strict water quality monitoring, (iii) environmental recovery (e.g., reforestation), and (iv) permanent protected areas. The objective of this paper is to verify the poor water quality in the Guandu and the ineffectiveness and undesired effects of Phoslock.

Conserved Pigment Profiles in Phylogenetically Diverse Symbiotic Bacteria Associated with the Corals Montastraea cavernosa and Mussismilia braziliensis.

Pigmented bacterial symbionts play major roles in the health of coral holobionts. However, there is scarce knowledge on the diversity of these microbes for several coral species. To gain further insights into holobiont health, pigmented bacterial isolates of Fabibacter pacificus (Bacteroidetes; n = 4), Paracoccus marcusii (Alphaproteobacteria; n = 1), and Pseudoalteromonas shioyasakiensis (Gammaproteobacteria; n = 1) were obtained from the corals Mussismilia braziliensis and Montastraea cavernosa in Abrolhos Bank, Brazil. Cultures of these bacterial symbionts produced strong antioxidant activity (catalase, peroxidase, and oxidase). To explore these bacterial isolates further, we identified their major pigments by HPLC and mass spectrometry. The six phylogenetically diverse symbionts had similar pigment patterns and produced myxol and keto-carotene. In addition, similar carotenoid gene clusters were confirmed in the whole genome sequences of these symbionts, which reinforce their antioxidant potential. This study highlights the possible roles of bacterial symbionts in Montastraea and Mussismilia holobionts.

Glacial-interglacial transitions in microbiomes recorded in deep-sea sediments from the western equatorial Atlantic.

In the late Quaternary, glacial-interglacial transitions are marked by major environmental changes. Glacial periods in the western equatorial Atlantic (WEA) are characterized by high continental terrigenous input, which increases the proportion of terrestrial organic matter (e.g. lignin, alkanes), nutrients (e.g. iron and sulphur), and lower primary productivity. On the other hand, interglacials are characterized by lower continental contribution and maxima in primary productivity. Microbes can serve as biosensors of past conditions, but scarce information is available on deep-sea sediments in the WEA. The hypothesis put forward in this study is that past changes in climate conditions modulated the taxonomic/functional composition of microbes from deep sediment layers. To address this hypothesis, we collected samples from a marine sediment core located in the WEA, which covered the last 130 kyr. This region is influenced by the presence of the Amazon River plume, which outputs dissolved and particulate nutrients in vast oceanic regions, as well as the Parnaiba river plume. Core GL-1248 was analysed by shotgun metagenomics and geochemical analyses (alkane, lignin, perylene, sulphur). Two clusters (glacial and interglacial-deglacial) were found based on taxonomic and functional profiles of metagenomes. The interglacial period had a higher abundance of genes belonging to several sub-systems (e.g. DNA, RNA metabolism, cell division, chemotaxis, and respiration) that are consistent with a past environment with enhanced primary productivity. On the other hand, the abundance of Alcanivorax, Marinobacter, Kangiella and aromatic compounds that may serve as energy sources for these bacteria were higher in the glacial. The glacial period was enriched in genes for the metabolism of aromatic compounds, lipids, isoprenoids, iron, and Sulphur, consistent with enhanced fluvial input during the last glacial period. In contrast, interglacials have increased contents of more labile materials originating from phytoplankton (e.g. Prochlorococcus). This study provides new insights into the microbiome as climatic archives at geological timescales.

A new genomic taxonomy system for the Synechococcus collective.

Cyanobacteria of the genus Synechococcus are major contributors to global primary productivity and are found in a wide range of aquatic ecosystems. This Synechococcus collective (SC) is metabolically diverse, with some lineages thriving in polar and nutrient-rich locations and others in tropical or riverine waters. Although many studies have discussed the ecology and evolution of the SC, there is a paucity of knowledge on its taxonomic structure. Thus, we present a new taxonomic classification framework for the SC based on recent advances in microbial genomic taxonomy. Phylogenomic analyses of 1085 cyanobacterial genomes demonstrate that organisms classified as Synechococcus are polyphyletic at the order rank. The SC is classified into 15 genera, which are placed into five distinct orders within the phylum Cyanobacteria: (i) Synechococcales (Cyanobium, Inmanicoccus, Lacustricoccus gen. Nov., Parasynechococcus, Pseudosynechococcus, Regnicoccus, Synechospongium gen. nov., Synechococcus and Vulcanococcus); (ii) Cyanobacteriales (Limnothrix); (iii) Leptococcales (Brevicoccus and Leptococcus); (iv) Thermosynechococcales (Stenotopis and Thermosynechococcus) and (v) Neosynechococcales (Neosynechococcus). The newly proposed classification is consistent with habitat distribution patterns (seawater, freshwater, brackish and thermal environments) and reflects the ecological and evolutionary relationships of the SC.

[Multiple exposure to pesticides and impacts on health: a cross-section study of 102 rural workers, Nova Friburgo, Rio de Janeiro State, Brazil]. / Exposição múltipla a agrotóxicos e efeitos à saúde: estudo transversal em amostra de 102 trabalhadores rurais, Nova Friburgo, RJ.

A cross section study was carried out in a farming community from Nova Friburgo, Rio de Janeiro state, Brazil, to examine epidemiological, clinical and laboratory aspects of multiple exposure to pesticides in a representative sample of 102 small farmers. Both males and females were submitted to an extensive protocol which included an occupation questionnaire, biological sample collection for toxicology analysis and clinical - general and neurological - evaluation. The toxicology test results showed light to moderate episodes of acute intoxication by organophosphorates either described by the farmers or observed during clinical examination. Thirteen cases of delayed neuropathies (12,8%) as well as 29 cases of neural behavioral syndromes and psychiatric disorders associated to the continued use of pesticides were diagnosed. These results indicate recurrent multiple overexposures to high concentrations of different chemicals, with serious damage to vital functions, especially considering their young age (average 35 +/- 11 years old) and the productive period in their lifetime. These results show how important it is to monitor multiple exposure to pesticides - a chain of events that may have major impacts on public health and the environment.

[Interictal brain SPECT in patients with medically refractory temporal lobe epilepsy]. / SPECT cerebral interictal em pacientes com epilepsia do lobo temporal de difícil controle.

The objective of this study was to evaluate the utility of interictal brain SPECT in localizing the epileptogenic focus in a population of patients of Epilepsy Clinic of Hospital Universitário Clementino Fraga Filho (HUCFF/UFRJ), with medically refractory temporal lobe epilepsy (TLE) and normal computed tomography (CT) scans, studying the correlation between SPECT, electroencephalogram (EEG) and, in 11 cases, brain magnetic resonance imaging (MRI), and to compare the results to the other six literature series. Twelve (52.2%) patients presented abnormal SPECT. Among these, five (41.6% of abnormal SPECTs) presented unilateral SPECT changes at the same side of EEG (hypoperfusion in four and hyperperfusion in one), three (25% of abnormal SPECTs) presented bilateral hypoperfusion and bilateral EEG changes too, and four (33.3%) presented unilateral hypoperfusion and bilateral EEG changes. The statistical analysis was based on fuzzy logic. The correlation index among SPECT X EEG, SPECT X MRI and SPECT X EEG X MRI were highly significant, with significance levels at 0.01, p < 0.0005 and trust interval at 99% in all correlations. The correlation studies between the series presented similar results.

SPECT cerebral interictal em pacientes com epilepsia do lobo temporal de difícil controle / SPECT cerebral interictal em pacientes com epilepsia do lobo temporal de difícil controleInterictal brain SPECT in patients with medically refractory temporal lobe epilepsy

O objetivo desse estudo foi avaliar a utilidade do SPECT cerebral interictal na localizaçäo do foco epileptogênico em 23 pacientes do Ambulatório de Epilepsias do Hospital Universitário Clementino Fraga Filho (HUCFF/UFRJ), com epilepsia do lobo temporal (ELT) de difícil controle e tomografia computadorizada (TC) do crânio normal, estudando a correlaçäo entre SPECT interictal, eletrencefalograma (EEG) e, em 11 casos, ressonância magnética (RM) do crânio, e comparar os resultados com os de outras seis séries da literatura. Doze (52,2 por cento) pacientes apresentaram SPECT anormal. Entre esses, cinco (41,6 por cento dos SPECTs anormais) apresentaram alteraçäo unilateral ao SPECT do mesmo lado do EEG (hipoperfusäo em 4 e hiperperfusäo em 1), três (25 por cento dos SPECTs anormais) apresentaram hipoperfusäo bilateral ao SPECT e alteraçöes também bilaterais ao EEG e 4 (33,3 por cento dos SPECTs anormais) apresentaram hipoperfusäo unilateral ao SPECT e EEG bilateral. A análise estatística fundamentou-se na lógica fuzzy. Os índices de correlaçäo entre SPECT X EEG, SPECT X RM e SPECT X EEG X RM foram altamente significativos, com níveis de significância de 0,01, p < 0,0005 e intervalo de 99 por cento de confiança em todas as correlaçöes. Os estudos de correlaçäo entre as séries estudadas apresentaram resultados semelhantes entre si

Valor del tránsito esofágico con Tc 99 en pacientes operados por acalasia de esófago / Value of esophageal transit with Tecnetium 99 in operated patients by esophageal achalasia

Se analiza un método radioisotópico de estudio para la acalasia de esófago, efectuado en el preoperatorio de 35 enfermos con operación de Heller modificada y en el postoperatorio de 21 de ellos. Se practicó tránsito esofágico con tecnecio 99, administrando 500 microcuries diluidos en 15 ml de agua por vía oral, con el paciente de pie. Se determinó el tiempo medio y retención a los 60 segundos; estos valores fueron elevados con respecto a los normales. No se pudieron establecer diferencias significativas entre estas cifras y la gravedad de la disfagia presente en el preoperatorio. Los valores de tiempo medio y retención en el postoperatorio se correspondieron con la mejoría clínica. En los buenos resultados se observó correlación entre los valores de presión del esfínter esofágico inferior y de tiempo medio y retención. Puede concluirse que el método radioisotópico con Tc 99 es una técnica no invasiva, comúnmente aceptada por el paciente y que permite evaluar en forma cuantitativa los resultados del tratamiento quirúrgico en la acalasia esofágica.

Evaluación del reflujo gastroesofágico mediante pHmetría intraesofágica ambulatoria de 24 horas / Ambulatory intraesophageal pHmetry for evalution of gastroesophageal reflux

Se evaluaron 20 pacientes con síntoma de reflujo gastroesofágico mediante pHmetría intraesofágica ambulatoria de 24 hs. Los resultados fueron comparados con aquellos obtenidos en 5 personas asintomáticas. Se estudió el número de reflujo, hora duración relativa del reflujo en porciento y duración promedio; hubo diferencias en los primeros valores y se estableció una buena correlación entre éstos y los hallazgos endoscópicos. En los pacientes con ausencia de esofagitis o inflamación leve de mucosa, los valores patológicos se observaron a predominio de la fase de vigilia, mientras que en aquellos con esofagitis moderadas y graves, los resultados anormales se presentaron tanto en vigilia como en sueño y en general el predominio de esta última. La pHmetría de 24 hs permite cuantificar la presencia de reflujo gastroesofágico en pacientes sintomáticos, enfatizando su utilidad en aquellos casos con síntomas típicos o atípicos y ausencia de manisfestaciones endoscópicas.

Valor del tránsito esofágico con Tc 99 en pacientes operados por acalasia de esófago / Value of esophageal transit with Tecnetium 99 in operated patients by esophageal achalasia

Se analiza un método radioisotópico de estudio para la acalasia de esófago, efectuado en el preoperatorio de 35 enfermos con operación de Heller modificada y en el postoperatorio de 21 de ellos. Se practicó tránsito esofágico con tecnecio 99, administrando 500 microcuries diluidos en 15 ml de agua por vía oral, con el paciente de pie. Se determinó el tiempo medio y retención a los 60 segundos; estos valores fueron elevados con respecto a los normales. No se pudieron establecer diferencias significativas entre estas cifras y la gravedad de la disfagia presente en el preoperatorio. Los valores de tiempo medio y retención en el postoperatorio se correspondieron con la mejoría clínica. En los buenos resultados se observó correlación entre los valores de presión del esfínter esofágico inferior y de tiempo medio y retención. Puede concluirse que el método radioisotópico con Tc 99 es una técnica no invasiva, comúnmente aceptada por el paciente y que permite evaluar en forma cuantitativa los resultados del tratamiento quirúrgico en la acalasia esofágica. (AU)

Evaluación del reflujo gastroesofágico mediante pHmetría intraesofágica ambulatoria de 24 horas / Ambulatory intraesophageal pHmetry for evalution of gastroesophageal reflux

Se evaluaron 20 pacientes con síntoma de reflujo gastroesofágico mediante pHmetría intraesofágica ambulatoria de 24 hs. Los resultados fueron comparados con aquellos obtenidos en 5 personas asintomáticas. Se estudió el número de reflujo, hora duración relativa del reflujo en porciento y duración promedio; hubo diferencias en los primeros valores y se estableció una buena correlación entre éstos y los hallazgos endoscópicos. En los pacientes con ausencia de esofagitis o inflamación leve de mucosa, los valores patológicos se observaron a predominio de la fase de vigilia, mientras que en aquellos con esofagitis moderadas y graves, los resultados anormales se presentaron tanto en vigilia como en sueño y en general el predominio de esta última. La pHmetría de 24 hs permite cuantificar la presencia de reflujo gastroesofágico en pacientes sintomáticos, enfatizando su utilidad en aquellos casos con síntomas típicos o atípicos y ausencia de manisfestaciones endoscópicas. (AU)