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1.
Hand Surg Rehabil ; 40(4): 389-393, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-33831625

RESUMO

Postoperative dressing protocols after clean surgery without implant vary widely. The purpose of this study was to elucidate whether early postoperative dressing removal is a valid option, as compared to untouched dressing or twice-weekly dressing change approach. A prospective randomized study was conducted on patients who underwent carpal tunnel release (CTR) or trigger finger release (TFR) between January and November 2020. Patients were randomly distributed into 3 groups: surgical dressing untouched until first follow up (SDU); surgical dressing changed twice a week in a health maintenance organization (HMO); and surgical dressing removed at first postoperative day (SDR). Data collected included patient characteristics, pre-and post-operative functional (QuickDASH) and autonomy (Instrumental Activities of Daily Living performance (IADL)) scores, Vancouver scar scale (VSS) and potential complications. Eighty-four patients were included: 28 (33.3%), 29 (34.5%) and 27 (32.1%) in the SDU, HMO and SDR groups, respectively. Deterioration in mean IADL score at 2-week follow-up was statistically significant in the HMO group (mean delta 3.35, p = 0.008). Quick DASH score improved significantly between preoperative and 2-week follow-up values only in the SDU group (mean delta 9.12, p = 0.012). Other parameters, including wound complications, did not differ significantly between groups. Early removal of postoperative dressing and immediate wound exposure was a safe option after CTR and TFR. An untouched bulky dressing correlated with early functional improvement. Finally, iterative dressing change in HMO showed no benefit and led to significant deterioration in early postoperative autonomy. IRB APPROVAL: 0548-18-TLV. LEVEL OF EVIDENCE: I.


Assuntos
Síndrome do Túnel Carpal , Mãos , Atividades Cotidianas , Bandagens , Síndrome do Túnel Carpal/cirurgia , Mãos/cirurgia , Humanos , Estudos Prospectivos
2.
Osteoporos Int ; 31(11): 2263-2267, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32561954

RESUMO

This is the first report describing three ipsilateral femoral stress fractures in a patient taking denosumab. INTRODUCTION: Multiple reports of atypical femur fractures (AFF) in patients receiving denosumab have emerged recently. Denosumab is an anti-resorptive agent approved for treatment of osteoporosis. It is a human monoclonal antibody which blocks osteoclast activation, maturation, and function. METHODS: This is a case report of a 74-year-old female patient who sustained three stress fractures of her left femur. RESULTS: The patient healed her fractures after intramedullary nailing of the femur and was able to return to her activities. CONCLUSIONS: High index of suspicion is needed in any patient with osteoporosis on denosumab complaining of thigh or groin pain. Careful examination and radiographic studies of both femurs are warranted if AFF is discovered.


Assuntos
Conservadores da Densidade Óssea , Denosumab , Fraturas do Fêmur , Fraturas de Estresse , Osteoporose , Idoso , Conservadores da Densidade Óssea/efeitos adversos , Denosumab/efeitos adversos , Difosfonatos , Feminino , Fraturas do Fêmur/induzido quimicamente , Fraturas do Fêmur/diagnóstico por imagem , Fraturas do Fêmur/cirurgia , Fêmur , Fraturas de Estresse/induzido quimicamente , Fraturas de Estresse/diagnóstico por imagem , Humanos , Osteoporose/tratamento farmacológico
3.
J Wound Care ; 24(10): 441-2, 444-5, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26488734

RESUMO

OBJECTIVE: Surgical site infection (SSI) after hip fracture surgery is a well-known complication with serious consequences for both the patient and the medical system. Silver ion treatment is considered an effective antibacterial agent, however, the use of silver dressing (SD) in the primary prevention of SSIs is controversial. The aims of this study were to compare SD with regular dressing (RD) in the prevention of SSI in elderly patients undergoing surgery for hip fractures, and to compare costs. METHOD: A matched group of 55 patients with hip fractures undergoing surgery with dynamic hip screw, cephalomedullary nail or hemiarthroplasty were randomised to either SD or RD groups. The dressings were applied in the operating theatre, and the patients were followed for one week for clinical signs of infection (discharge, erythema and fever). The RDs were replaced daily. The SDs were not removed for 5-7 days and kept moist. Skin swabs were taken from the wound surface on postoperative day 5-7 for bacterial skin colonisation. RESULTS: The SD (n=31) and RD (n=24) groups were similar in age, sex and comorbidities. Infection signs were seen in two (2/31, 6.4%) of the SD patients compared with 2 (2/24, 8.3%) RD patients (p=1.0). Skin colonisation by bacteria at postoperative day 5-7 was tested in 27 patients: it was higher in the SD group (positive skin swab, 12/19, 63.2%) compared to the RD group (4/8, 50%, p=0.67). The use of SD added ~US$5 (UK ~£3.19) per patient. CONCLUSION: The use of SD was associated with higher costs than RD, but not superior in preventing SSIs in elderly patients undergoing hemiarthroplasty or fixation of hip fractures. SD was also not effective in reducing bacterial skin colonisation following hip fracture and surgery.


Assuntos
Antibacterianos/uso terapêutico , Bandagens , Fraturas do Quadril/complicações , Fraturas do Quadril/cirurgia , Compostos de Prata/uso terapêutico , Infecção da Ferida Cirúrgica/etiologia , Infecção da Ferida Cirúrgica/prevenção & controle , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Estudos Prospectivos , Resultado do Tratamento , Cicatrização
4.
Clin Exp Immunol ; 182(2): 220-9, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26212048

RESUMO

Adoptive T cell therapy of cancer employs a large number of ex-vivo-propagated T cells which recognize their targets either by virtue of their endogenous T cell receptor (TCR) or via genetic reprogramming. However, both cell-extrinsic and intrinsic mechanisms often diminish the in-vivo potency of these therapeutic T cells, limiting their clinical efficacy and broader use. Direct activation of human T cells by Toll-like receptor (TLR) ligands induces T cell survival and proliferation, boosts the production of proinflammatory cytokines and augments resistance to regulatory T cell (Treg) suppression. Removal of the TLR ligand-binding region results in constitutive signalling triggered by the remaining cytosolic Toll/interleukin-1 receptor (TIR) domain. The use of such TIR domains therefore offers an ideal means for equipping anti-tumour T cells with the arsenal of functional attributes required for improving current clinical protocols. Here we show that constitutively active (ca)TLR-4 can be expressed efficiently in human T cells using mRNA electroporation. The mere expression of caTLR-4 mRNA in polyclonal CD8 and CD4 T cells induced the production of interferon (IFN)-γ, triggered the surface expression of CD25, CD69 and 4-1BB and up-regulated a panel of cytokines and chemokines. In tumour-infiltrating lymphocytes prepared from melanoma patients, caTLR-4 induced robust IFN-γ secretion in all samples tested. Furthermore, caTLR-4 enhanced the anti-melanoma cytolytic activity of tumour-infiltrating lymphocytes and augmented the secretion of IFN-γ, tumour necrosis factor (TNF)-α and granulocyte-macrophage colony-stimulating factor (GM-CSF) for at least 4 days post-transfection. Our results demonstrate that caTLR-4 is capable of exerting multiple T cell-enhancing effects and can potentially be used as a genetic adjuvant in adoptive cell therapy.


Assuntos
Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD8-Positivos/imunologia , RNA Mensageiro/imunologia , Receptor 4 Toll-Like/imunologia , Antígenos CD/imunologia , Antígenos CD/metabolismo , Antígenos de Diferenciação de Linfócitos T/imunologia , Antígenos de Diferenciação de Linfócitos T/metabolismo , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/metabolismo , Linhagem Celular Tumoral , Células Cultivadas , Quimiocinas/imunologia , Quimiocinas/metabolismo , Citocinas/imunologia , Citocinas/metabolismo , Eletroporação , Citometria de Fluxo , Expressão Gênica/imunologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos/imunologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos/metabolismo , Humanos , Interferon gama/imunologia , Interferon gama/metabolismo , Subunidade alfa de Receptor de Interleucina-2/imunologia , Subunidade alfa de Receptor de Interleucina-2/metabolismo , Células K562 , Lectinas Tipo C/imunologia , Lectinas Tipo C/metabolismo , Linfócitos do Interstício Tumoral/imunologia , Linfócitos do Interstício Tumoral/metabolismo , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismo , Transfecção/métodos , Membro 9 da Superfamília de Receptores de Fatores de Necrose Tumoral/imunologia , Membro 9 da Superfamília de Receptores de Fatores de Necrose Tumoral/metabolismo , Fator de Necrose Tumoral alfa/imunologia , Fator de Necrose Tumoral alfa/metabolismo
5.
Cryobiology ; 44(1): 54-61, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12061848

RESUMO

The objective of this work was to confirm the main role of elastic fibers in differing responses of certain vessels during cooling from 37 to 8 degrees C. Previous results have shown that the nature of the vessel (conduit vessel vs muscular vessel) determines the different behavior (dilatation vs contraction) of isolated vessel segments when temperature decreases from 37 to 8 degrees C. In this work, it has been demonstrated that vessels with a great amount of elastic fibers show a dilatation when cooling. On the other hand, muscular vessels with fewer elastic fibers, such as the renal artery, undergo a contraction. The output of calcium from intracellular stores causes contraction of the renal artery during cooling. In this vessel, vasodilatation occurs only when mechanisms of smooth muscle contraction are inactive, as is the case with vessels that have undergone a cold storage period of 48 h. The results presented in this work confirm that there are two main effects, which directly depend on the vessel origin. In conduit arteries, the decrease of temperature induces a vascular relaxation, dependent on the elastic component of the vessel wall. In muscular vessels, the predominant effect is cooling-induced contraction due to an increase of intracellular calcium. This cooling-induced contraction needs the vessel to be in optimal conditions with an active metabolism of the muscular cells. These results are a crucial issue in the sense of explaining several biomedical mechanisms where hypothermia is implicated. The type of vessel implicated in procedures, such as isolated organ perfusion, extracorporeal circulation, and bypass surgery, must be taken into account.


Assuntos
Tecido Elástico/fisiologia , Hipotermia Induzida , Vasodilatação/fisiologia , Animais , Aorta Torácica/fisiologia , Temperatura Baixa , Técnicas In Vitro , Masculino , Ratos , Ratos Wistar , Artéria Renal/fisiologia , Suínos , Porco Miniatura , Vasoconstrição/fisiologia
6.
Artigo em Inglês | MEDLINE | ID: mdl-11563034

RESUMO

LC/MS assays were developed to determine the plasma and intracellular concentrations of two aryl phosphoramidate prodrugs of the nucleotide analog 9-[2-R-(phosphonomethoxy)propyl]adenine. LC/MS was used to demonstrate the presence of high concentrations of PMPA in peripheral blood mononucleocytes following oral administration of prodrugs in dogs. High concentrations of PMPA and active metabolite were detected in MT-2 cells incubated with prodrug using an ion-pairing LC/MS assay.


Assuntos
Adenina/análogos & derivados , Adenina/sangue , Amidas/sangue , Leucócitos Mononucleares/metabolismo , Organofosfonatos , Compostos Organofosforados/sangue , Ácidos Fosfóricos/sangue , Pró-Fármacos/análise , Animais , Linhagem Celular , Cromatografia Líquida , Cães , Humanos , Espectrometria de Massas , Tenofovir
7.
Artif Organs ; 24(11): 899-902, 2000 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11119079

RESUMO

The main goal of this work was to study the influence of perfusion pressure and flow waveform during kidney perfusion, and the relationship between renal vascular resistance (RVR) and lactate dehydrogenase (LDH) concentration in the perfusate. Simultaneous constant pressure kidney perfusions were performed with either pulsatile or continuous flow at either 30 or 80 mm Hg of constant perfusion pressure. Mean flow, pressure, and RVR were displayed online during perfusion. Perfusate samples for LDH, creatine phosphatase kinase (CPK), and alkaline phosphatase (AP) determinations were taken. At the end of the perfusion, 2 ml of Evans blue was injected into the circuit to obtain images of perfusate distribution, and the kidneys were weighed. Also, hematoxylin/eosine studies were performed, showing more Bowman's space and tubular dilation in kidneys perfused with high pressure. We did not find differences in RVR between kidneys perfused at 30 and 80 mm Hg; nevertheless, perfusate distribution was better in the 80 mm Hg perfusions. We did not find any correlation between enzyme release and RVR in kidneys perfused with different mean pressures. These findings suggest that vascular resistance and LDH concentration cannot be independently considered as adequate markers of perfusate distribution.


Assuntos
Rim/enzimologia , L-Lactato Desidrogenase/análise , Preservação de Órgãos/métodos , Perfusão/métodos , Resistência Vascular/fisiologia , Fosfatase Alcalina/análise , Animais , Biomarcadores/análise , Corantes , Creatina Quinase/análise , Análise Fatorial , Rim/irrigação sanguínea , Glomérulos Renais/anatomia & histologia , Túbulos Renais/anatomia & histologia , Sistemas On-Line , Tamanho do Órgão , Pressão , Fluxo Pulsátil , Distribuição Aleatória , Reologia , Suínos , Porco Miniatura
8.
Cryobiology ; 41(1): 43-50, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11017760

RESUMO

Temperature may have significant influence on vascular tone in such cases as organ preservation, coronary bypass surgery, and extracorporeal circulation. The aim of this research was to study the direct effect of temperature variation on vascular tone in an attempt to elucidate the mechanisms involved. In a first series of experiments, the isometric tension of two different vessels (rat thoracic aorta and pig renal branch artery) was studied at different temperatures. To study the role of calcium in this response, a second series of experiments was performed. In this the vessels were incubated with the intracellular chelator BAPTA/AM. Further experiments were performed to test the effect of cold storage. Our results show that changes in temperature lead to different results in pig renal artery and rat aorta. A decrease in temperature induced a highly reproducible relaxation in rat aorta, whereas pig renal artery presented cooling-induced contraction. Moreover, whereas calcium depletion failed to inhibit cooling-induced relaxation in rat aorta, it did not provoke cooling-induced contraction in pig renal artery. Similar responses were obtained with cold storage and calcium depletion. We intend to demonstrate that, just as the effect of temperature variation on pig renal artery is due to a metabolic mechanism, its effect on rat aorta may be due to structural factors. This hypothesis is supported by the result of histological studies which demonstrate a higher proportion of elastin fibres in rat aorta than in pig renal artery.


Assuntos
Músculo Liso Vascular/fisiologia , Temperatura , Resistência Vascular/fisiologia , Animais , Aorta Torácica/química , Aorta Torácica/efeitos dos fármacos , Aorta Torácica/fisiologia , Cálcio/fisiologia , Quelantes/farmacologia , Criopreservação , Ácido Egtázico/análogos & derivados , Ácido Egtázico/farmacologia , Elastina/análise , Contração Isométrica/efeitos dos fármacos , Contração Isométrica/fisiologia , Masculino , Relaxamento Muscular/efeitos dos fármacos , Relaxamento Muscular/fisiologia , Músculo Liso Vascular/química , Músculo Liso Vascular/efeitos dos fármacos , Ratos , Ratos Wistar , Artéria Renal/química , Artéria Renal/efeitos dos fármacos , Artéria Renal/fisiologia , Reprodutibilidade dos Testes , Suínos , Porco Miniatura , Resistência Vascular/efeitos dos fármacos
9.
J Fam Pract ; 43(5): 495-8, 1996 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8917150

RESUMO

Henoch-Schönlein purpura (HSP) is a systemic, generalized vasculitis of unknown etiology thought to be related to an IgA-mediated autoimmune phenomenon. Diagnosis is based on a constellation of physical findings that include the characteristic nonthrombocytopenic petechial or purpuric rash, migratory polyarthralgias, abdominal pain, and renal complications. We report the case of a 19-year-old man with a diagnosis of HSP who had severe abdominal pain and endoscopic documentation of duodenal involvement. Though not clear at presentation, the diagnosis became obvious when the characteristic rash emerged.


Assuntos
Vasculite por IgA/diagnóstico , Adulto , Diagnóstico Diferencial , Humanos , Vasculite por IgA/tratamento farmacológico , Masculino , Esteroides/uso terapêutico
10.
Am J Med ; 100(1): 85-9, 1996 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-8579092

RESUMO

PURPOSE: In adults, discitis most frequently follows spinal surgery. We report 16 adult patients with spontaneously occurring infectious discitis and compare them with an additional 52 patients abstracted from the literature. Infecting organisms, predisposing factors, imaging modalities, and response to therapy are described. PATIENTS AND METHODS: The medical records of adult patients treated for infectious discitis of a community hospital during the past 10 years were reviewed. Postoperative spine patients and patients with primary osteomyelitis were excluded. Sixteen patients were identified with spontaneous primary infection of the disc space. The particulars of comorbid conditions, infection organisms, site of culture, and response to antibiotic therapy were noted and compared to 52 additional cases of spontaneous discitis reported in the literature since 1980. RESULTS: A wide variety of infecting organisms was identified as causing spontaneous discitis, in contrast to previous reports of both postoperative discitis and spontaneous discitis. Nine of 10 patients with positive disc cultures had negative blood cultures. Appropriate antibiotics were curative in all patients but 1, regardless of the duration of symptoms. Nuclear imaging, computed tomography, and magnetic resonance imaging were all useful, although the last appears to be the most sensitive and specific imaging modality for detecting discitis. CONCLUSIONS: Spontaneous infectious discitis is an uncommon cause of low back pain in adults. Nevertheless, it should be considered in any patient with acute or subacute pain. Elevated acute-phase reactants with appropriate imaging modality suggest the diagnosis. given the wide variety of infecting organisms identified, culture of blood and/or disc for the specific causative organism is critical to successful treatment outcome.


Assuntos
Infecções Bacterianas , Discite/microbiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Antibacterianos/uso terapêutico , Bacteriemia/microbiologia , Infecções Bacterianas/diagnóstico , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/microbiologia , Vértebras Cervicais/microbiologia , Comorbidade , Discite/diagnóstico , Discite/tratamento farmacológico , Feminino , Hospitais Comunitários , Humanos , Dor Lombar/diagnóstico , Vértebras Lombares/microbiologia , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Sensibilidade e Especificidade , Tomografia Computadorizada por Raios X , Resultado do Tratamento
11.
Hosp Pract (Off Ed) ; 27(6): 119-22, 127-31, 1992 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-1597489

RESUMO

A case is made for the existence of a primary antiphospholipid antibody syndrome. This clinical entity can be characterized as including an elevation in titers of antibodies to negatively charged phospholipids, hypercoagulation, thrombocytopenia, and recurrent fetal loss. With further elucidation, the systemic pathologic roles of antiphospholipid antibodies should emerge.


Assuntos
Síndrome Antifosfolipídica , Aborto Habitual/etiologia , Anticorpos Anti-Idiotípicos/sangue , Síndrome Antifosfolipídica/sangue , Síndrome Antifosfolipídica/complicações , Síndrome Antifosfolipídica/tratamento farmacológico , Feminino , Humanos , Lúpus Eritematoso Sistêmico/sangue , Fosfolipídeos/imunologia , Gravidez , Complicações Hematológicas na Gravidez
12.
Am J Nephrol ; 12(1-2): 126-33, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1492877

RESUMO

Postpartum hemolytic uremic syndrome (HUS) is described in a woman with a history of spontaneous abortions and both circulating lupus anticoagulant and anticardiolipin antibody (ACA). After termination of her pregnancy because of severe preeclampsia, ACA blood levels increased simultaneously with the onset of a microangiopathic process associated with severe hypertension and renal failure. Plasma exchange resulted in a rapid decline in ACA levels and immediate improvement in her clinical condition. This case strongly suggests an important causal relationship between ACA and postpartum HUS. The possible mechanisms of ACA-related postpartum HUS and the potential role of plasmapheresis in its treatment are reviewed and discussed.


Assuntos
Anticorpos Anticardiolipina/sangue , Síndrome Antifosfolipídica/complicações , Síndrome Hemolítico-Urêmica/etiologia , Transtornos Puerperais/etiologia , Adulto , Síndrome Antifosfolipídica/sangue , Feminino , Síndrome Hemolítico-Urêmica/sangue , Síndrome Hemolítico-Urêmica/terapia , Humanos , Troca Plasmática , Gravidez , Transtornos Puerperais/sangue , Transtornos Puerperais/terapia
16.
Infect Immun ; 28(1): 295-7, 1980 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-6769819

RESUMO

Scrub typhus immunogens that received inadequate gamma radiation contained residual, viable rickettsiae. The presence of these organisms in the host was masked by the rapid immune response elicited by the large number of inactivated rickettsiae. Transfer of homogenized spleen cells from immunized mice to normal syngeneic recipients provided a sensitive technique for the detection of these viable, replicating organisms.


Assuntos
Antígenos de Bactérias , Técnicas Bacteriológicas , Orientia tsutsugamushi/imunologia , Baço/microbiologia , Animais , Feminino , Raios gama , Camundongos , Camundongos Endogâmicos C3H , Orientia tsutsugamushi/crescimento & desenvolvimento , Orientia tsutsugamushi/efeitos da radiação , Baço/transplante , Transplante Homólogo
17.
Bull Am Acad Psychiatry Law ; 8(1): 44-55, 1980.
Artigo em Inglês | MEDLINE | ID: mdl-7225588

RESUMO

The serious question of involuntary hospitalization at this point in history is properly referred to due process in court. While numerous studies have addressed the decisions, statistics and outcomes in this area, insufficient attention has been paid to how the clinician may productively incorporate the clinical experience mobilized by the procedure into the therapeutic work. The authors suggest that the issues raised include maintenance of the alliance, responsibility, dependence, limit-setting, the subjective experience of the process, and the problem of counter-transference. They indicate how the various aspects of commitment may be explored aso as to yield maximal therapeutic benefit, and maintain the alliance during the conflicts of an adversary procedure.


Assuntos
Internação Compulsória de Doente Mental/legislação & jurisprudência , Psiquiatria Legal/legislação & jurisprudência , Transtornos Mentais/terapia , Adulto , Comunicação , Contratransferência , Feminino , Humanos , Masculino , Relações Profissional-Paciente , Estados Unidos
19.
Infect Immun ; 26(1): 131-6, 1979 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-115796

RESUMO

Scrub typhus immunogens were prepared from Rickettsia tsutsugamushi strains Karp, Kato, Gilliam, Kostival, and Buie by exposing frozen infected yolk sac suspensions to 300 krad of gamma radiation. Mouse protection tests showed that each of the irradiated immunogens protected C3H/HeDub mice against high challenge levels of Karp and Gilliam, but that none of these single-strain immunogens were capable of protecting against all five of the challenge strains. Broad-spectrum protection was achieved by using combinations of three strains of irradiated rickettsiae in a vaccination regimen of three injections at 5-day intervals. A comparison of vaccination efficacy employing three such combinations (Karp-Gilliam-Kato, Karp-Kostival-Kato, and Buie-Kostival-Kato) indicated that both sequential administration of strains on successive vaccination days and multiple injections of trivalent mixtures produced protective responses superior to those obtained with single-strain immunogens. Trivalent mixtures of rickettsiae exhibited a striking synergistic effect on the immune response of C3H/HeDub mice and elicited a protective response against Kato challenge that could not be obtained with any single-strain immunogen. Mice vaccinated with the trivalent Karp-Gilliam-Kato immunogen resisted challenge with more than 10(3) 50% mouse lethal doses of Karp and Gilliam for 12 months, and were resistant to similar levels of challenge with Kato and Buie for 6 months.


Assuntos
Orientia tsutsugamushi/imunologia , Vacinas Antirrickéttsia , Tifo por Ácaros/imunologia , Vacinas , Animais , Feminino , Raios gama , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Orientia tsutsugamushi/efeitos da radiação , Fatores de Tempo , Vacinação
20.
Infect Immun ; 22(1): 298-300, 1978 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-103827

RESUMO

Unirradiated Rickettsia tsutsugamushi and a component of gamma-irradiated Karp immunogen required for homologous immunity were more stable than the immunogen component that elicited heterologous (Kato strain) protection.


Assuntos
Antígenos de Bactérias , Orientia tsutsugamushi/imunologia , Animais , Congelamento , Raios gama , Camundongos , Orientia tsutsugamushi/patogenicidade , Orientia tsutsugamushi/efeitos da radiação , Tifo por Ácaros/imunologia , Temperatura
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