RESUMO
BACKGROUND: We explored the correlation between the TGFBR2 gene that is mediated by NF-κb signaling pathways and the pathogenesis of Kawasaki disease in children. METHODS: In this study, 43 children with Kawasaki disease from April 2014 to January 2016 at our hospital were selected as the observation group, and 42 healthy children were selected as the control group. The mRNA expression levels of NF-κb gene and TGFBR2 gene in different groups were detected using fluorescence quantitative PCR. The protein expression levels of the NF-κb and TGFBR2 were detected using enzyme-linked immunosorbent assay (ELISA) in different groups. The expression levels of NF-κb and TGFBR2 in the observation group and the control group were detected using immunohistochemistry. RESULTS: Compared to the control group, the mRNA expression levels of NF-κb and TGFBR2 were 12.3 times and 27.5 times as high as those in the control group respectively and there were significant differences between the two groups (P<0.05). ELISA results showed that there were significant differences between the protein expression levels of NF-κb and TGFBR2 in the control group (0.87±0.12, 1.25±0.18 µg/L) and those in the observation group (3.27±0.17, 8.16±0.22 µg/L) (P<0.05). Western-blotting results showed that the expression levels of the NF-κB and TGFBR2 in children with Kawasaki disease were significantly higher than those in healthy subjects (P<0.05). Immunohistochemistry results showed that the positive cell rate of TGFBR2 (89.7%) was significantly higher in children with Kawasaki disease than that in healthy children (4.5%); there was significant difference between the two groups (P<0.05). CONCLUSIONS: The TGFBR2 may be involved in the pathogenesis of Kawasaki disease in children through NF-κb signaling pathways.
Assuntos
Síndrome de Linfonodos Mucocutâneos/fisiopatologia , NF-kappa B/metabolismo , Receptor do Fator de Crescimento Transformador beta Tipo II/genética , Transdução de Sinais/genética , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Feminino , Regulação da Expressão Gênica , Humanos , Imuno-Histoquímica , Masculino , Síndrome de Linfonodos Mucocutâneos/genética , NF-kappa B/genética , RNA Mensageiro/metabolismoRESUMO
This study aims to evaluate the effect of peripheral blood miR-125b expression on severity and prognosis in children with viral encephalitis (VE). Children with VE (severe and mild groups) were grouped into VE group, and 40 healthy children as control group. Plasma RNA was extracted, and real-time quantitative PCR was conducted to detect miR-125b relative expression. Associations of miR-125b expression with clinical characteristics and prognosis of VE children were analyzed. Area under ROC curve (AUC) was calculated to evaluate the accuracy of the prognostic value of miR-125b. Univariate analysis and logistic regression analysis were performed to analyze risk factors of the prognoses of VE children. The plasma miR-125b expression was higher in the VE group than in the control group and higher in the severe group than the mild group. MiR-125b expression was associated with status convulsion, hemiplegia, multiple organ injuries, and stress hyperglycemia in VE children. Patients with poor prognosis exhibited higher miR-125b expression than those with good prognosis, and the rate of high miR-125b expression of the patients with poor prognosis (64.10%, 25/39) was higher than that in those with good prognosis (28.92%, 24/83). The AUC of miR-125b expression to predict prognosis of VE children was 0.833. When the cutoff value was 1.715, the diagnostic sensitivity (87.2%), specificity (71.1%), and accuracy (76.2%) were the highest. Status convulsion, stress hyperglycemia, and miR-125b were considered as risk factors for poor prognosis in VE children. Peripheral blood miR-125b expression may be correlated with the severity and prognosis of VE in children.
Assuntos
Encefalite Viral/sangue , Encefalite Viral/diagnóstico , MicroRNAs/sangue , Adolescente , Criança , Pré-Escolar , Progressão da Doença , Encefalite Viral/terapia , Feminino , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Lactente , Masculino , MicroRNAs/genética , RNA Mensageiro , Curva ROC , Índice de Gravidade de DoençaRESUMO
Transforming growth factor type ?1(TGF—?1)belong to a family of polypeptides with regulato-ry effects on growth and differention of a variety of cells types.TGF—?1 play an important rolein regulation of immune response by acting as a negative modulate the cell proliferation throughstill unknow mechanisms.We investigated the effect of TGF—?1 on the IL—2R expression andproliferation activited DN cells and we observed that TGF—?1 was able to increase the expres-sion of IL—2R in DN cells and to suppress the proliferation of DN cells and we have discussedabout these results.