RESUMO
BACKGROUND: Preventing post-harvest waste of Palmyra palm (Borassus aethiopum Mart.) fruits is possible by recovery of pectin as a value-added ingredient. Extraction conditions on yield and functionality of Palmyra palm pectin was determined at different temperatures and pH values with 30 min extraction time. RESULTS: Palmyra palm fruits contain more than 650 g kg-1 galacturonic acid and produce soft gels with sucrose in acidic media despite a high degree of acetylation (â¼5%). Mechanical deformation of pectin gel was similar when extracted at pH 2.5 and 70 °C or under natural pH at room temperature or 70 °C. Pectins isolated at pH 7 exhibited comparable gel softness (G'/Gâ³) with commercial pectin. Palm pectins also showed emulsifying activity greater than 50%, attributed to high protein content of 8 g 100 g-1 . For pectins extracted at pH near 5.2-5.5, molar mass ranged from 3.00 to 3.38 × 105 g mol-1 ; intrinsic viscosity ranged from 218 to 297 mL g-1 ; arabinose was the main neutral sugar; ζ-potential ranged from -23 to -25 mV. CONCLUSION: Palm fruit offers an inexpensive raw material to extract pectin in environmentally friendly and economical way and yield a pectin with unique gelling, viscosifying and emulsifying properties. © 2016 Society of Chemical Industry.
Assuntos
Arecaceae/química , Frutas/química , Pectinas/química , Extratos Vegetais/química , Biotecnologia , Concentração de Íons de Hidrogênio , Peso Molecular , Pectinas/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Temperatura , ViscosidadeRESUMO
Separate methods for the analyses of soluble carbohydrates in different plants and simmondsins in jojoba seed meal are described. A reliable gas chromatographic procedure for the simultaneous quantification of D-pinitol, myo-inositoL sucrose, 5-alpha-D-galactopyranosyl-D-pinitol. 2-alpha-D-galactopyranosyl-D-pinitol, simmondsin, 4-demethylsimmondsin, 5-demethylsimmondsin and 4,5-didemethylsimmondsin as trimethylsilyl derivatives in jojoba seed meal has been developed. The study of different extraction mixtures allowed for the quantitative recovery of the 9 analytes by a mixture of methanol-water (80:20, v/v) in the concentration range between 0.1 and 4%. Comparison of the separation parameters on three different capillary stationary phases with MS detection allowed for the choice of the optimal gas chromatographic conditions for baseline separation of the analytes.