RESUMO
Changes in the secondary structure of enzymes induced by gamma-rays 60Co at doses not exceeding one ionization per macromolecule were studied to elucidate a possible role of radiation-chemical processes in the evolution of proteins. The data on the comparative radioresistance of various types of secondary protein structures, alpha-helix, parallel and anti-parallel beta-structures, and beta-turn, were obtained by the method of circular dichroism. It was shown that beta-turns were resistant against radiation, alpha-helix was relatively stable, and beta-layer underwent significant changes. The importance of these structural types in the evolution of proteins is discussed. A special role of beta-turn as structural elements fixing the confirmation of macromolecules and therefore responsible for adaptation of the protein structure against a constant radiation background is proposed.
Assuntos
Adaptação Fisiológica/efeitos da radiação , Álcool Desidrogenase/efeitos da radiação , L-Lactato Desidrogenase/efeitos da radiação , Estrutura Secundária de Proteína/efeitos da radiação , Ribonucleases/efeitos da radiação , Subtilisinas/efeitos da radiação , Animais , Bovinos , Dicroísmo Circular , Relação Dose-Resposta à Radiação , Raios gama , Cavalos , Fígado/enzimologia , Músculos/enzimologia , Pâncreas/enzimologia , SuínosRESUMO
S-Methylmethionine had moderate radioprotective activity, the efficiency of radioprotection was 15-30%, the value of dose decrease coefficient-1.2. Biological effect of S-methylmethionine was probably provided by its ability to decrease the level of lipid peroxidation and inhibit monoamine oxidase activity.
Assuntos
Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/farmacologia , Vitamina U/farmacologia , Animais , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos da radiação , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/efeitos da radiação , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Lesões Experimentais por Radiação/metabolismo , Lesões Experimentais por Radiação/mortalidadeRESUMO
The change in the secondary structure of ribonuclease after 60Co gamma irradiation with a dose of 1,000 Gy in 0.2% aqueous solution was estimated using the circular dichroism method. The beta structures were significantly changed, while other types of the secondary structure (alpha-helix and beta-turns) changed insignificantly. The secondary structure injury was also affected by oxygen. The data are attributed to characteristics of the secondary structure of this enzyme.
Assuntos
Oxigênio/farmacologia , Ribonucleases/efeitos da radiação , Animais , Bovinos , Dicroísmo Circular , Raios gama , Pâncreas/enzimologia , Conformação Proteica/efeitos dos fármacos , Conformação Proteica/efeitos da radiação , Ribonucleases/química , Ribonucleases/efeitos dos fármacos , Soluções , VácuoRESUMO
A simultaneous synthesis of peptides (2-5 residues) and oligonucleotides (3-9 residues) has been carried out on caolinite matrix using amino acids and aminoacyladenylates as substrates. The rate of oligomer synthesis on mineral surface is higher than that in solution. The mechanism of synthesis has been described. The data has been discussed in connection with abiogenesis of two major types of biopolymers, proteins and nucleic acids.
Assuntos
Oligonucleotídeos/síntese química , Peptídeos/síntese química , Monofosfato de Adenosina/análogos & derivados , Aminoácidos , Concentração de Íons de Hidrogênio , Hidrólise , Técnicas In Vitro , Caulim , Oligonucleotídeos/análise , Peptídeos/análise , TemperaturaAssuntos
Melaninas/farmacocinética , Peptídeos/síntese química , Polímeros/farmacocinética , Evolução Biológica , Catálise , Melaninas/efeitos da radiação , Peptídeos/efeitos da radiação , Fotoquímica , Polímeros/efeitos da radiação , Sílica Gel , Dióxido de Silício/farmacocinética , Dióxido de Silício/efeitos da radiação , Raios UltravioletaAssuntos
Oxigênio/efeitos da radiação , Ribonucleases/efeitos da radiação , Animais , Bovinos , Relação Dose-Resposta à Radiação , Ativação Enzimática/efeitos da radiação , Raios gama , Técnicas In Vitro , Oxigênio/metabolismo , Pâncreas/enzimologia , Desnaturação Proteica/efeitos da radiação , Ribonucleases/metabolismo , SoluçõesRESUMO
The monolayer technique, the methods of electron microscopy and IR-spectroscopy were used to study isolated nuclear membranes of calf thymus cells. The data obtained permitted to study the relationship between structural disorders induced by irradiation of membranes, the changes in their functional status, and the role played by a lipid component of membranes in these processes.
Assuntos
Núcleo Celular/efeitos da radiação , Membranas Intracelulares/efeitos da radiação , Animais , Núcleo Celular/fisiologia , Núcleo Celular/ultraestrutura , Fenômenos Químicos , Físico-Química , Membranas Intracelulares/fisiologia , Membranas Intracelulares/ultraestrutura , Lipídeos de Membrana/efeitos da radiação , Microscopia Eletrônica , Fosfolipídeos/efeitos da radiação , Espectrofotometria Infravermelho , TermodinâmicaRESUMO
A comparative study was made of the effect of X-radiation on the membrane-bound glucoso-6-phosphatase of the nuclear membrane and microsomal fraction of calf thymus cells. Dose- and concentration-dependencies of inactivation of glucoso-6-phosphatase are indicative of a higher radiosensitivity of glucoso-6-phosphatase of nuclear membranes than that of microsomes. This difference in radiosensitivity is associated with the peculiarities of the composition and structural organization of these two membrane systems of a cell.
Assuntos
Glucose-6-Fosfatase/efeitos da radiação , Membranas Intracelulares/metabolismo , Animais , Bovinos , Núcleo Celular/metabolismo , Glucose-6-Fosfatase/metabolismo , Técnicas In Vitro , Microssomos/metabolismo , Tolerância a Radiação , Timo/ultraestruturaRESUMO
A study was made of the effect of X-radiation on nuclear membranes. Good resolution spectra of nuclear membrane of equal effective thickness were obtained by the method of internal reflection spectroscopy in the infrared region. The experiment indicated the presence of major characteristic bands of protein and phospholipid molecular groups of non-irradiated nuclear membranes. Conformational changes in the samples of the exposed (100 and 1000 Gy) lipoprotein complex resulted in some quantitative changes in the absorption intensities of the molecular groups of membranes. These data are consistent with the behaviour of nuclear membranes on the interphase, studied by the Langmur monolayer technique, and with the changes in the membrane morphology observed with the electron microscope.
Assuntos
Membrana Nuclear/efeitos da radiação , Animais , Bovinos , Técnicas In Vitro , Lipídeos de Membrana/efeitos da radiação , Proteínas de Membrana/efeitos da radiação , Conformação Proteica/efeitos da radiação , Espectrofotometria Infravermelho , Timo/citologiaRESUMO
The ribonuclease molecules irradiated in a solution in the presence of 18O2 were separated, by the method of gel-chromatography, into fractions of aggregated macromolecules, denatured monomers and the molecules which retained the original sizes. Oxygen was bound by the molecules of all fractions. The oxygen binding prevented the aggregation of macromolecules. The fractions of molecules of monomer forms, obtained after irradiation in the presence of oxygen, were more inactive than those obtained after irradiation in vacuum.
Assuntos
Oxigênio/farmacologia , Ribonucleases/efeitos da radiação , Animais , Bovinos , Cromatografia por Troca Iônica , Raios gama , Técnicas In Vitro , Substâncias Macromoleculares , Pâncreas/enzimologia , Desnaturação Proteica/efeitos da radiação , Ribonucleases/antagonistas & inibidores , Ribonucleases/metabolismoRESUMO
Kinetics of the process has been investigated employing differential spectroscopy technique. Possibility of synthesis of peptide bonds in aqueous solutions using aminoacyladenylates as condensing agents was shown. Analysis of the data obtained shows that the synthesis of peptide bond under the conditions investigated with the excess amounts of the second substrate follow the first order equation for irreversible reactions, the rate of the reaction decreasing with the increase in substrate concentrations. Velocity constants are determined for the first order reaction at different substrate concentrations. The data obtained are discussed in relation to the problem of abiogenic synthesis of biologically important macromolecules.
Assuntos
Monofosfato de Adenosina/análogos & derivados , Peptídeos/síntese química , Monofosfato de Adenosina/farmacologia , Aminoácidos/farmacologia , Relação Dose-Resposta a Droga , Técnicas In Vitro , Cinética , Substâncias Macromoleculares , Soluções , Espectrofotometria Ultravioleta , Água/farmacologiaAssuntos
Adenosina Trifosfatases/efeitos da radiação , Fosfatase Alcalina/efeitos da radiação , Ribonucleases/efeitos da radiação , Timo/enzimologia , Animais , Bovinos , Relação Dose-Resposta à Radiação , Ativação Enzimática/efeitos da radiação , Técnicas In Vitro , Magnésio , Membrana Nuclear/enzimologia , Membrana Nuclear/efeitos da radiação , Raios XRESUMO
It has been demonstrated that UV irradiation of acetaldehyde (2.5%) and ammonium nitrate (1.5%) solutions results in the synthesis of the pyruvic acid (PA). With the increase in the duration of irradiation, the yield of the produced PA decreases which is associated both with its photolysis and with its further transformations, for instance, into amino acids. The results obtained are discussed in relation to abiogenic synthesis of biologically important compounds in prebiological evolution and to possible development of metabolic pathways which are found in contemporary organisms.