Adapting a continuous flow cryostat and a plate DAC to do high pressure Raman experiments at low temperatures.

We present a method for modifying a continuous flow cryostat and a steel plate DAC (Diamond Anvil Cell) to perform high pressure micro-Raman experiments at low temperatures. Despite using a steel DAC with a lower specific heat capacity (∼335 J/kg K), this setup can routinely perform high pressure (∼10 GPa) measurements at temperatures as low as 26 K. This adaptation is appropriate for varying the temperature of the sample while keeping it at a constant pressure. We determined that the temperature variation across the sample chamber is about 1 K using both direct temperature measurements and finite element analysis of the heat transport across the DAC. We present Raman spectroscopy results on elemental selenium at high pressures and low temperatures using our modified setup.

Dose controlled low energy electron irradiator for biomolecular films.

We have developed a multi target, Low Energy Electron (LEE), precise dose controlled irradiator for biomolecular films. Up to seven samples can be irradiated one after another at any preset electron energy and dose under UHV conditions without venting the chamber. In addition, one more sample goes through all the steps except irradiation, which can be used as control for comparison with the irradiated samples. All the samples are protected against stray electron irradiation by biasing them at -20 V during the entire period, except during irradiation. Ethernet based communication electronics hardware, LEE beam control electronics and computer interface were developed in house. The user Graphical User Interface to control the irradiation and dose measurement was developed using National Instruments Lab Windows CVI. The working and reliability of the dose controlled irradiator has been fully tested over the electron energy range of 0.5 to 500 eV by studying LEE induced single strand breaks to ΦX174 RF1 dsDNA.