[Action of amsidine on the evolution of lymphocyte cultures and on the structure of human metaphase chromosomes]. / Action de l'amsidine sur l'évolution de cultures lymphocytaires et sur la structure des chromosomes métaphasiques humains.

Chromosomic alterations can be observed after the effect of a antimitotic amsidine on metaphases of cultured human lymphocytes: gaps, breaks, exchange figures, deletions, rings. Moreover we notice, among these cells, particular lines of big sized lymphoplasmocytoid cells, whose rate is abnormally high when compared to reference cultures.

[In vitro study of fibroblasts from patients with Duchenne muscular dystrophy. Evaluation of capping and ultrastructural aspect]. / Etude in vitro de fibroblastes de malades atteints de myopathie de Duchenne: évaluation du capping et aspect ultrastructural.

There have been conflicting studies of lymphocyte capping from patients with Duchenne Muscular Dystrophy. We have evaluated the proportion of capped fibroblasts of 10 patients with Duchenne Muscular Dystrophy. The results, compared with 15 normal controls, showed that the reduction of fibroblast capping is correlated to age patients. Additional studies, decreased number of retracted fibroblasts after colchicine incubation and ultrastructural observations, suggest that capping deficit would be the consequence of microtubular system alteration. This late phenomenon may be secondary to the primary membrane defect.

[Characterization of chromosomal aberrations induced in man by various antimitotic agents]. / Typologie d'aberrations chromosomiques induites chez l'Homme par différents antimitotiques.

Six antitumoral drugs were tested in human lymphocytes cultures. Our observations pointed out specific abnormalities beside more usual chromosomal aberrations. These lesions are mainly telomeric and chromatidic fusions, chromomerisations and despiralizations. We present an interpretation of these abnormalities.

[Immunocytochemical labeling of human chromosomes by antibodies from human autoimmune serum]. / Marquage immunocytochimique de chromosomes humains après action d'anticorps de sérum autoimmun d'origine humaine.

Mitotic human chromosomes are successively irradiated by ultraviolet, incubated with serum from a patient showing systemic lupus erythematosus (S.L.E.), and antihuman Sheep serum conjugated with peroxidase. After revelation by diaminobenzidine (DAB) banding patterns are pointed out. The chromosome labelling is discussed in comparison with results obtained in other immunocytochemical experiments.

[Variations of various enzymatic activities of the Krebs cycle (malate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase) during experimental ischemic shock in the rat. Influence of adenosine 5' triphosphoric acid]. / Variations de quelques activités enzymatiques du cycle de Krebs (malate déshydrogénase, isocitrate déshydrogénase, succinate déshydrogénase) au cours du choc ischémique expérimental chez le rat. Influence de l'acide adénosine-5' triphosphorique

During the ischemic shock caused by the removal of tourniquets placed on the hind paws of the rat, a marked decrease in the enzyme activities of Krebs cycle yielding ATP (malate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase) at the level of the gastrocnemius muscle and the liver, was observed together with a plasma increase of these enzymes. The intraperitoneal injection of ATP diminishes significantly the variations observed.