RESUMO
The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth.
Assuntos
Intestino Delgado/citologia , Lactação , Celulas de Paneth/citologia , Animais , Feminino , CobaiasRESUMO
El objetivo del estudio fue determinar el tiempo máximo para recuperar y criopreservar espermatozoides obtenidos de la cola del epidídimo de caninos post orquiectomía. Se obtuvo testículos de 20 caninos entre 1 y 8 años de edad. Los testículos se colocaron en cloruro de sodio al 0.9% y se almacenaron a 5 ºC durante 0, 24, 48 y 72 horas. Los espermatozoides fueron recuperados al cortar la cola del epidídimo en un dilutor en base a Tris-citrato-fructosa. Para el proceso de criopreservación, se añadió yema de huevo (20%) y glicerol (5%) a la muestra diluida (espermatozoides + dilutor). La nueva dilución fue envasada en pajillas de 0.5 ml y sometidas a una curva de enfriamiento para luego sercolocadas en nitrógeno líquido. Se evaluó motilidad total, motilidad progresiva e integridad funcional de membrana. Previo a la criopreservación, la motilidad total varió significativamente después de 24 y 72 horas de almacenamiento a 5 ºC (p<0.05). Sin embargo, la motilidad total encontrada después del proceso de criopreservación sólo varió después de 72 horas de almacenamiento a 5 ºC (p<0.05). Similar tendencia se observó para la motilidad progresiva. La integridad funcional de membrana plasmática, tanto antes como después del proceso de criopreservación, no sufrió cambios significativos entre los grupos. Los resultados demuestran que es posible recuperar y criopreservar espermatozoides epididimarios hasta después de 48 horas de la orquiectomía del animal.
The aim of the study was to determine the maximum time to recover and cryopreserve spermatozoa from the tail of canine epididymis post orchiectomy. The testes were obtained through orchiectomy from 20 dogs aged 1 to 8 years. The testis were placed in sodium chloride 0.9% and stored at 5 °C for 0, 24, 48 and 72 hours. Spermatozoa were recovered by cutting the tail of the epididymis in an extender based on Tris-citrate-fructose. For the cryopreservation process, egg yolk (20%) and glycerol (5%) was added to the diluted sample (sperm + dilutor).The new dilution was packaged in 0.5 ml straws, which were subjected to a cooling curve and then placed in liquid nitrogen. Total motility, progressive motility and functional integrity of the membrane were evaluated. Before cryopreservation process, total motility after 24 and 72 hours of storage at 5 ºC decreased (p<0.05). However, total motility found after cryopreservation process varied significantly only after 72 hours of storage at 5 ºC (p<0.05). Similar trend was observed for progressive motility. The functional integrity of membrane, both before and after the cryopreservation process, did not suffer significant changes between groups. The results showed that it is possible to collect and cryopreserve epididymal sperm until 48 hours of orchiectomy.
Assuntos
Animais , Criopreservação , Cães , Epididimo , Espermatozoides , Orquiectomia , Preservação do SêmenRESUMO
Se estudió el efecto del ketoprofeno, un antiprostaglandínico bloqueador de la enzima ciclooxigenasa, sobre la presión arterial pulmonar media (PAPm) en 10 terneros Jersey machos, de 1 a 2 meses de edad, nacidos a nivel del mar y expuestos durante 3 días a una hipoxia ambiental de 3,320 m de altitud. Los terneros se distribuyeron en un grupo de 5 animales que recibió placebo (grupo control) y un grupo que recibió ketoprofeno en dosis de 3 mg/kg de peso vivo, por 5 días consecutivos, durante su estadía a nivel del mar (grupo tratado). La PAPm se determinó mediante la técnica de cateterismo cardiaco a nivel del mar y al tercer día de su estadía en la altura. Los valores promedio de la PAPm (mm Hg) a nivel del mar fueron de 19.00 ± 0.94 para el Grupo control y 21.00 ± 3.39 para el Grupo tratado, y en la altura de 39.50 ± 6.00 para el Grupo control y 31.25 ± 5.70 para el Grupo tratado, no existiendo diferencia significativa. Sin embargo, se observó diferencia por exposición a la altura al analizar la información de ambos grupos en conjunto (a nivel del mar: 20.00 ± 2.68 y al tercer día de exposición a la altura: 35.28 ± 7.16) (p menor que 0.05). Estos resultados sugieren que el ketoprofeno no tuvo efecto sobre la PAPm en terneros Jersey sometidos durante 3 días a la hipoxia de la altura.
The effect of ketoprofen, an antiprostaglandin substance that inhibits the enzyme ciclooxigenase, on the mean pulmonary arterial pressure (mPAP) was evaluated in 10, male Jersey calves, of 1-2 months of age. Calves were born at sea level and exposed during 3 days to an environmental hypoxia of 3,320 m of high altitude. A group of 5 animals received destiled water as placebo (Control group), and other group received ketoprofen in dose of 3 mg/kg body weight for 5 consecutive days while at sea level (Treated group). The mPAP was measured by cardiac cateterism at sea level and 3 days after arriving to the high altitude. The mean values of mPAP (mm Hg) at sea level were: Control group 19.00 ± 0.94 and Treated group 21.00 ± 3.39; whereas at the high altitude were: Control group 39.50 ± 6.00 and Treated group 31.25 ± 5.70, and without significant differences. When data of the two groups were joined and analysed together, the values for mPAP at sea level (20.00 ± 2.68) were statistically different (p minor that 0.05) from those at day 3 of exposition to high altitude (35.28 ± 7.16). The results suggested that ketoprofen had not effect on mPAP in Jersey calves exposed during 3 days at high altitude hypoxia.