RESUMO
Papers dealing with the study of the immune response to dengue virus infection and with the role it may play in the pathogenesis of dengue hemorrhaghic fever are increasingly important. The memory human T cell response in individuals with history of dengue infection during the Cuban epidemic was studied in this paper for a further evaluation of the antigenicity of viral proteins. To this end, mononuclear cells of peripheral blood from individuals immune to dengue and from a group of control subjects with viral antigens were incubated. It was obtained a significant proliferative response of lymphocytes from individuals with history of infection against dengue virus type 2 compared with control subjects. It was proved this way the dengue virus-specific memory T CD4+ cell response in the individuals under study.
Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Anticorpos Antivirais , Antígenos Virais/imunologia , Dengue , Vírus da Dengue , Linfócitos T , Antígenos Virais/sangueRESUMO
The cellular immune response to dengue type 2 virus envelope protein was studied. To this end, the lympho-proliferative capacity of T-lymphocytes obtained from splenocytes of animals immunized with the protein when they were stimulated by such protein and dengue 2 virus. It was realized that splenocytes proliferated significantly in response to both types of viral antigens and that the values of stimulation indexes were higher in response to the whole virus than to the protein alone. Based on the above-mentioned, it was concludes that purified dengue 2 virus envelope protein was capable of generating specific and memory responses of antigen T-cell to dengue 2 type virus.