The effect of cannabis edibles on driving and blood THC.

BACKGROUND: Cannabis has been shown to impact driving due to changes produced by delta-9-tetrahydrocannabinol (THC), the psychoactive component of cannabis. Current legal thresholds for blood THC while driving are based predominantly on evidence utilizing smoked cannabis. It is known that levels of THC in blood are lower after eating cannabis as compared to smoking yet the impact of edibles on driving and associated blood THC has never been studied. METHODS: Participants drove a driving simulator before and after ingesting their preferred legally purchased cannabis edible. In a counterbalanced control session, participants did not consume any THC or cannabidiol (CBD). Blood was collected for measurement of THC and metabolites as well as CBD. Subjective experience was also assessed. RESULTS: Participants consumed edibles with, on average, 7.3 mg of THC, which is less than the maximum amount available in a single retail package in Ontario, providing an ecologically valid test of cannabis edibles. Compared to control, cannabis edibles produced a decrease in mean speed 2 h after consumption but not at 4 and 6 h. Under dual task conditions in which participants completed a secondary task while driving, changes in speed were not significant after the correction for multiple comparison. No changes in standard deviation of lateral position (SDLP; 'weaving'), maximum speed, standard deviation of speed or reaction time were found at any time point or under either standard or dual task conditions. Mean THC levels were significantly increased, relative to control, after consuming the edible but remained relatively low at approximately 2.8 ng/mL 2 h after consumption. Driving impairment was not correlated with blood THC. Subjective experience was altered for 7 h and participants were less willing/able to drive for up to 6 h, suggesting that the edible was intoxicating. INTERPRETATION: This is the first study of the impact of cannabis edibles on simulated driving. Edibles were intoxicating as revealed by the results of subjective assessments (VAS), and there was some impact on driving. Detection of driving impairment after the use of cannabis edibles may be difficult.

Producer perceptions of dairy calf management, behavior, and welfare.

Approaches for raising calves vary across commercial dairy farms and relate to behavioral opportunities and animal welfare. The objectives of this study were to evaluate how US dairy producers and calf managers perceive 1) welfare implications of varying management practices (including social housing and milk allowance) and behaviors in dairy calves, and 2) aspects of the human-animal relationship in calf rearing and relationships with on-farm management and personal calf handling habits. Survey questions were primarily quantitative (e.g., Likert scales) and addressed how common calf management practices and observed calf behaviors were related to aspects of welfare, including calf health and comfort. We additionally posed questions addressing respondent habits, management protocols, and perceptions related to human-animal interaction. Responses from 93 dairy producers and calf managers were collected via digital surveys. Social housing was viewed as being generally positive for both calf comfort and health, although this view was stronger with respect to calf comfort. Respondents from farms using social housing (56%) had more positive perceptions of social housing, viewed social play as being associated with better calf comfort and health, and considered access to other calves and "freedom to express natural behavior" as being more important for calves, compared with respondents from farms not providing social housing. Providing greater milk allowances (>7.6 L/d) was viewed as being good for both calf comfort and health, although respondents from farms providing these milk allowances (59%) had more positive perceptions than those who provided lesser allowances. Abnormal oral behaviors were viewed as being associated with both poor calf comfort and health. The welfare importance of various resources which may reduce abnormal oral behaviors (including hay and brushes) was perceived more ambiguously, although respondents from farms providing these resources, compared with those who do not, generally viewed them as more preferred by calves. We observed a positive relationship between how respondents perceived the human-animal bond (i.e., that calves enjoy contact with humans) and stated personal behavior related to calf contact (frequency of contacting calves to scratch or pet them). Respondent demographics were not related to perceptions of the human-animal relationship, but respondents identifying as female described more frequent positive calf interactions. Described aspects of human-animal interactions were not related to implementation of social housing on-farm. Job satisfaction was positively related to perception of the human-animal relationship. Overall, these results suggest that most calf management personnel place a high value on calf welfare, although farms implementing social housing appear to place a greater value on subjective calf well-being and individual perceptions of animal welfare may depend on practical experience.

Characterizing ammonia emissions from horses fed different crude protein concentrations.

Evaluating impact of animal agriculture on air quality has been the focus of recent research. Ammonia (NH) volatilization occurs when undigested protein in feces and urea in urine is broken down by bacteria and enzymes. Information regarding NH emission from equine facilities is limited, and effects of CP intake on NH emissions have not been investigated. Nine mature geldings were used in a 3 × 3 replicated Latin square design study to determine effects of dietary CP on potential NH losses from feces and urine. We hypothesized feeding horses above the CP requirement would result in an increase in NH emissions from urine and feces and different bedding materials would affect NH emissions from urine. Diets were formulated using different ratios of bahiagrass () and Tifton-85 bermudagrass () hays, and a commercial vitamin mineral supplement to provide 3 different CP concentrations and labeled in relation to each other: LOW-CP, MED-CP, and HIGH-CP (10.6%, 11.5%, and 12%, respectively). Each study period consisted of an 11-d diet adaptation phase, followed by a 3-d total collection of urine and feces. To determine total nitrogen (TN) and urea-N concentrations, samples were pooled by period ( = 9). For in vitro determination of NH concentrations, urine and fecal samples were pooled within period by diet ( = 3) and mixed with either wheat straw or wood shavings. Ammonia emission of these samples was measured using a vessel system with an airflow rate (2.5 L/min) at 20°C over a 7-d period. Concentration of NH in each vessel was measured using a photoacoustic multigas analyzer. Temperature, airflow rate, and NH concentration in each vessel were used to calculate NH emission rate (ER). Data were analyzed using a mixed model ANOVA with repeated measures. Urinary TN and urea-N excretion increased as CP intake increased ( < 0.0001). Vessel urinary NH concentrations were not different across diets ( = 0.1225), ranging from 55.48 ppm (LOW-CP) to 101.14 ppm (HIGH-CP); however, they differed between bedding types ( < 0.0001), with straw higher than shavings (97 vs. 73.5 ppm, respectively). Cumulative urinary NH ER tended to be different across diets ( = 0.0550) ranging from 5.87 g/m to 9.97 g/m and bedding types ( = 0.0129), with straw being higher than shavings (11.1 vs. 6.9 g/m, respectively). Overfeeding CP to horses can lead to increased urinary TN and urea-N excretion, which could lead to greater of NH in the atmosphere.

Organization of the neural switching circuitry underlying reflex micturition.

The functions of the lower urinary tract to store and periodically eliminate urine are regulated by a complex neural control system in the brain and spinal cord that coordinates the activity of the bladder and urethral outlet. Experimental studies in animals indicate that urine storage is modulated by reflex mechanisms in the spinal cord, whereas voiding is mediated by a spinobulbospinal pathway passing through a coordination centre in the rostral brain stem. Many of the neural circuits controlling micturition exhibit switch-like patterns of activity that turn on and off in an all-or-none manner. This study summarizes the anatomy and physiology of the spinal and supraspinal micturition switching circuitry and describes a computer model of these circuits that mimics the switching functions of the bladder and urethra at the onset of micturition.

Crib-biting in US horses: breed predispositions and owner perceptions of aetiology.

REASONS FOR PERFORMING STUDY: Crib-biting is an equine stereotype that may result in diseases such as colic. Certain breeds and management factors have been associated. OBJECTIVES: To determine: breed prevalence of crib-biting in US horses; the likelihood that one horse learns to crib-bite from another; and owner perceptions of causal factors. METHODS: An initial postal survey queried the number and breed of crib-biting horses and if a horse began after being exposed to a horse with this habit. In a follow-up survey, a volunteer subset of owners was asked the number of affected and nonaffected horses of each breed and the extent of conspecific contact. The likelihood of crib-biting given breed and extent of contact was quantified using odds ratio (OR) and significance of the association was assessed using the Chi-squared test. RESULTS: Overall prevalence was 4.4%. Thoroughbreds were the breed most affected (133%). Approximately half of owners believed environmental factors predominantly cause the condition (54.4%) and crib-biting is learned by observation (48.8%). However, only 1.0% of horses became affected after being exposed to a crib-biter. The majority (86%) of horses was turned out in the same pasture with other horses and extent of contact with conspecifics was not statistically related to risk. CONCLUSION: This is the first study to report breed prevalence for crib-biting in US horses. Thoroughbreds were the breed more likely to be affected. More owners believed either environmental conditions were a predominant cause or a combination of genetic and environmental factors contributes to the behaviour. Only a small number of horses reportedly began to crib-bite after being exposed to an affected individual, but approximately half of owners considered it to be a learned behaviour; most owners did not isolate affected horses. POTENTIAL RELEVANCE: Genetic predisposition, not just intensive management conditions and surroundings, may be a factor in the high crib-biting prevalence in some breeds, and warrants further investigation. Little evidence exists to suggest horses learn the behaviour from other horses, and isolation may cause unnecessary stress.

Pathway HUDs: are they viable?

We describe two experiments that examine 3D pathway displays in a head-up location for aircraft landing and taxi. We address both guidance performance and pilot strategies in dividing, focusing, and allocating attention between flight path information and event monitoring. In Experiment 1 the 3D pathway head-up display (HUD) was compared with a conventional 2D HUD. The former was found to produce better guidance, with few costs to event detection. Some evidence was provided that attentional tunneling of the pathway HUD inhibits the detection of unexpected traffic events. In Experiment 2, the pathway display was compared in a head-up versus a head-down location. Excellent guidance was achieved in both locations. A slight HUD cost for vertical tracking in the air was offset by a HUD benefit for event detection and for lateral tracking during taxi (i.e., on the ground). The results of both experiments are interpreted within the framework of object- and space-based theories of visual attention and point to the conclusion that pathway HUDs combine the independent advantages of pathways and HUDs, particularly during ground operations. Actual or potential applications include understanding the costs and benefits of positioning a 3D pathway display in a head-up location.

Infrequent genetic exchange and recombination in the mitochondrial genome of Candida albicans.

Previous analyses of diploid nuclear genotypes have concluded that recombination has occurred in populations of the yeast Candida albicans. To address the possibilities of clonality and recombination in an effectively haploid genome, we sequenced seven regions of mitochondrial DNA (mtDNA) in 45 strains of C. albicans from human immunodeficiency virus-positive patients in Toronto, Canada, and 3 standard reference isolates of C. albicans, CA, CAI4, and WO-1. Among a total of 2,553 nucleotides in the seven regions, 62 polymorphic nucleotide sites and seven indels defined nine distinct mtDNA haplotypes among the 48 strains. Five of these haplotypes occurred in more than one strain, indicating clonal proliferation of mtDNA. Phylogenetic analysis of mtDNA haplotypes resulted in one most-parsimonious tree. Most of the nucleotide sites undergoing parallel change in this tree were clustered in blocks that corresponded to sequenced regions. Because of the existence of these blocks, the apparent homoplasy can be attributed to infrequent, past genetic exchange and recombination between individuals and cannot be attributed to parallel mutation. Among strains sharing the same mtDNA haplotypes, multilocus nuclear genotypes were more similar than expected from a random comparison of nuclear DNA genotypes, suggesting that clonal proliferation of the mitochondrial genome was accompanied by clonal proliferation of the nuclear genome.

Attentional filtering in the design of electronic map displays: a comparison of color coding, intensity coding, and decluttering techniques.

In a series of experiments color coding, intensity coding, and decluttering were compared in order to assess their potential benefits for accessing information from electronic map displays. Participants viewed electronic battlefield maps containing 5 classes of information discriminable by color or intensity, or, in the decluttering condition, displayed or removed entirely by a key press. Participants were asked questions requiring them to focus on objects within a class (objects presented at the same color or intensity) or to integrate data between objects in different classes (objects presented at different colors and intensities). The results suggested that the benefits of color and intensity coding appear to be in segregating the visual field rather than calling attention to the objects presented at a certain color or intensity. Interactivity proved to be a disadvantage; the time cost of information retrieval outweighed the time benefits of presenting less information on the display or even allowing map users to customize their displays. Potential applications of this research include a cost-benefit analysis for the use of 3 attentional filtering techniques and an attempt to quantitatively measure map complexity.

Display signaling in augmented reality: effects of cue reliability and image realism on attention allocation and trust calibration.

This experiment seeks to examine the relationships among three advanced technology features (presentation of target cuing, reliability of target cuing, and level of image reality and the attention and trust given to that information). The participants were 16 military personnel who piloted an unmanned air vehicle and searched for targets camouflaged in terrain, which was presented at two levels of image realism. Cuing was available for some targets, and the reliability of this information was manipulated at two levels (100% and 75%). The results showed that the presence of cuing aided target detection for expected targets but drew attention away from the presence of unexpected targets. Cuing benefits and attentional tunneling were both reduced when cuing became less reliable. Increasing image realism was compelling but increased reliance on the cuing information when those data were reliable. Potential applications include a cost-benefit analysis of how trust modulates attention in the use of automated target recognition systems and the extent to which increased realism may influence this trust.

A model for types and levels of human interaction with automation.

Technical developments in computer hardware and software now make it possible to introduce automation into virtually all aspects of human-machine systems. Given these technical capabilities, which system functions should be automated and to what extent? We outline a model for types and levels of automation that provides a framework and an objective basis for making such choices. Appropriate selection is important because automation does not merely supplant but changes human activity and can impose new coordination demands on the human operator. We propose that automation can be applied to four broad classes of functions: 1) information acquisition; 2) information analysis; 3) decision and action selection; and 4) action implementation. Within each of these types, automation can be applied across a continuum of levels from low to high, i.e., from fully manual to fully automatic. A particular system can involve automation of all four types at different levels. The human performance consequences of particular types and levels of automation constitute primary evaluative criteria for automation design using our model. Secondary evaluative criteria include automation reliability and the costs of decision/action consequences, among others. Examples of recommended types and levels of automation are provided to illustrate the application of the model to automation design.

Frames of reference for the display of battlefield information: judgment-display dependencies.

In 2 experiments, U.S. Army soldiers viewed computer-generated displays that presented battlefield information from 3 different frames of reference: a 2D plan view display (with contour lines), a 3D exocentric perspective display, and an interactive 3D immersed display. In Experiment 1, soldiers made geographical judgments. The results suggested that both 3D displays suffered from ambiguity of distance estimates but that the 3D immersed display was most accurate for judging whether a location is directly visible from another. In Experiment 2, the 3D exocentric display was compared with a 3D immersed view, coupled with a small 2D inset map, in a more continuous battlefield scenario in which judgments of enemy activity were made. The findings of 3D ambiguity were replicated from Experiment 1. The accuracy of judgments of enemy activity suffered with the immersed display when information necessary to answer correctly did not appear in the initial forward view and required panning to acquire, reflecting the cognitive demands of integration across different views. This display also hindered soldiers' ability to report changes in enemy activity from one scene to the next. The results of this research will help to provide guidelines for the appropriate choice of computer display technology to assist in designing battlefield visualization aids. Caution should be exercised in choosing immersive viewpoints.

Target cuing in visual search: the effects of conformality and display location on the allocation of visual attention.

Two experiments were performed to examine how frame of reference (world-referenced vs. screen-referenced) and target expectancy can modulate the effects of target cuing in directing attention for see-through helmet-mounted displays (HMDs). In the first experiment, the degree of world referencing was varied by the spatial accuracy of the cue; in the second, the degree of world referencing was varied more radically between a world-referenced HMD and a hand-held display. Participants were asked to detect, identify, and give azimuth information for targets hidden in terrain presented in the far domain (i.e., the world) while performing a monitoring task in the near domain (i.e., the display). The results of both experiments revealed a cost-benefit trade-off for cuing such that the presence of cuing aided the target detection task for expected targets but drew attention away from the presence of unexpected targets in the environment. Analyses support the observation that this effect can be mediated by the display: The world-referenced display reduced the cost of cognitive tunneling relative to the screen-referenced display in Experiment 1; this cost was further reduced in Experiment 2 when participants were using a hand-held display. Potential applications of this research include important design guidelines and specifications for automated target recognition systems as well as any terrain-and-targeting display system in which superimposed symbology is included, specifically in assessing the costs and benefits of attentional cuing and the means by which this information is displayed.

Head-up displays: effects of clutter, display intensity, and display location on pilot performance.

Two experiments examined the effects of display location (head-up and head-down), display clutter, and display intensity on pilot performance in a general aviation-cruise flight environment. In Experiment 1, a low-fidelity simulation revealed that the detection of commanded flight changes and flight-path tracking performance was better in the head-down condition as compared to the head-up condition. In contrast, midair traffic detection was superior with the head-up display (HUD), reflecting an attentional trade-off. Experiment 2 used the same paradigm in a high-fidelity visual simulation. Flight performance was equivalent between HUD and head-down locations. Detection of commanded changes and traffic was better in the HUD condition, revealing the HUD benefits of reduced scanning. The presence of clutter inhibited detection of command changes and traffic in both head-up and head-down conditions. Lowlighting the task-irrelevant clutter did not facilitate detection of commanded changes, however, the clutter cost for detecting traffic was diminished if the added information was lowlighted in the head-down location. The data suggested that attention was modulated between tasks (flight control and detection), and between display areas (head-up and head-down).

Visual scanning and pilot expertise: the role of attentional flexibility and mental model development.

In order to examine differences in flying expertise, 12 novice and 12 expert pilots flew a 7-segment simulation pattern under specific attentional constraints while cockpit instrument visual scan was recorded. Flight segments involved various combinations of maneuvering of heading, altitude and airspeed. Expert pilots performed better than novices on vertical and longitudinal, but not lateral control. They accomplished their superior vertical tracking by allocating more control resources to the vertical control. Analyses of scanning strategies revealed that experts: a) had shorter dwells and more frequent visits to most instruments; b) adapted their visiting strategy more flexibly in response to changing task demands; c) demonstrated a better mental model of cross-coupling and predictive relations between and within axes; and d) showed more frequent checking of axes whose values remained constant. The data is discussed in terms of their implications in pilot cockpit scan training program development.

Information access in a dual-task context: testing a model of optimal strategy selection.

Pilots were required to access information from a hierarchical aviation database by navigating under single-task conditions (Experiment 1) and when this task was time-shared with an altitude-monitoring task of varying bandwidth and priority (Experiment 2). In dual-task conditions, pilots had 2 viewports available, 1 always used for the information task and the other to be allocated to either task. Dual-task strategy, inferred from the decision of which task to allocate to the 2nd viewport, revealed that allocation was generally biased in favor of the monitoring task and was only partly sensitive to the difficulty of the 2 tasks and their relative priorities. Some dominant sources of navigational difficulties failed to adaptively influence selection strategy. The implications of the results are to provide tools for jumping to the top of the database, to provide 2 viewports into the common database, and to provide training as to the optimum viewport management strategy in a multitask environment.

Superimposition, symbology, visual attention, and the head-up display.

In two experiments we examined a number of related factors postulated to influence head-up display (HUD) performance. We addressed the benefit of reduced scanning and the cost of increasing the number of elements in the visual field by comparing a superimposed HUD with an identical display in a head-down position in varying visibility conditions. We explored the extent to which the characteristics of HUD symbology support a division of attention by contrasting conformal symbology (which links elements of the display image to elements of the far domain) with traditional instrument landing system (ILS) symbology. Together the two experiments provide strong evidence that minimizing scanning between flight instruments and the far domain contributes substantially to the observed HUD performance advantage. Experiment 1 provides little evidence for a performance cost attributable to visual clutter. In Experiment 2 the pattern of differences in lateral tracking error between conformal and traditional ILS symbology supports the hypothesis that, to the extent that the symbology forms an object with the far domain, attention may be divided between the superimposed image and its counterpart in the far domain.

Electronic maps for terminal area navigation: effects of frame of reference and dimensionality.

Two experiments are reported that contrast rotating versus fixed electronic map displays, which pilots used for a simulated approach to a landing. In Experiment 1, a rotating versus fixed-map display was experimentally crossed with a two-dimensional (2D) versus three-dimensional (3D) view (perspective map) as pilots' ability to maintain the flight path and demonstrate awareness of the location of surrounding terrain features were assessed. Rotating displays supported better flight path guidance and did not substantially harm performance on terrain awareness tasks. 3D displays led to a substantial cost for vertical control but did not differ from 2D displays in lateral control. In Experiment 2, pilots flew with the rotating 2D display and with an improved version of the rotating 3D display, designed to reduce the ambiguity of representing altitude information. Vertical control improved as a result of the 3D display design improvement, but lateral control did not. The results are discussed in terms of the costs and benefits of presenting information in 3D, ego-referenced format for both flight path control and terrain awareness.

Role of multiple resources in predicting time-sharing efficiency: evaluation of three workload models in a multiple-task setting.

The goal of our study was to assess the validity of the assumptions underlying three prominent workload models: the Time-Line Analysis and Prediction workload model (Parks & Boucek, 1989), the VACP workload model (Aldrich, Szabo, & Bierbaum, 1989), and the W/INDEX model (North & Riley, 1989). Sixteen subjects flew a low-fidelity flight simulation. Subjects were required to perform a two-axis tracking task, a concurrent visual-monitoring task, and a discrete decision task. The decision task had 16 variations defined by two levels on each of the following dimensions: input modality (visual vs. auditory), processing code (spatial vs. verbal), difficulty (easy vs. hard), and response modality (manual vs. voice). Dual-task costs were found only for the tracking task. The tracking data were then analyzed using two approaches: a traditional analysis of variance (ANOVA) and a correlational analysis of tracking performance versus model predictions. The ANOVA revealed that performance on the tracking task was better when the concurrent decision task was responded to vocally and was easy. Input modality and processing code of the concurrent decision task had no significant effect on tracking performance. The correlational analysis was used to evaluate each of the three models, to determine what features were responsible for improving the models' fit, and to compare their performance with a pure time-line model that makes no multiple-resource assumptions. All three models did a good job of predicting variance between experimental conditions, accounting for between 56% and 84% of the variance in our data and between 10% and 40% of an earlier data set. Different features of each model that affect the fit are then discussed. We conclude that it is important for models to retain a multiple-resource coding, although the best features of that coding remain to be determined. Coding tasks by their demand level appears to be less critical.

Mental workload and cognitive task automaticity: an evaluation of subjective and time estimation metrics.

The evaluation of mental workload is becoming increasingly important in system design and analysis. The present study examined the structure and assessment of mental workload in performing decision and monitoring tasks by focusing on two mental workload measurements: subjective assessment and time estimation. The task required the assignment of a series of incoming customers to the shortest of three parallel service lines displayed on a computer monitor. The subject was either in charge of the customer assignment (manual mode) or was monitoring an automated system performing the same task (automatic mode). In both cases, the subjects were required to detect the non-optimal assignments that they or the computer had made. Time pressure was manipulated by the experimenter to create fast and slow conditions. The results revealed a multi-dimensional structure of mental workload and a multi-step process of subjective workload assessment. The results also indicated that subjective workload was more influenced by the subject's participatory mode than by the factor of task speed. The time estimation intervals produced while performing the decision and monitoring tasks had significantly greater length and larger variability than those produced while either performing no other tasks or performing a well practised customer assignment task. This result seemed to indicate that time estimation was sensitive to the presence of perceptual/cognitive demands, but not to response related activities to which behavioural automaticity has developed.

Implications of graphics enhancements for the visualization of scientific data: dimensional integrality, stereopsis, motion, and mesh.

This study examines the effectiveness of four techniques to assist scientists in evaluating multidimensional data. Subjects viewed a series of complex 3D data sets, each representing an underlying complex surface, from which a set of discrete points or observations were sampled. From each sample they answered questions that required focus of attention on certain data points or integration across varying numbers of data points and dimensions. After a number of samples were viewed from each surface, subjects were tested for their retention of the surface characteristics. In Experiment 1, 3D (perspective) representations were found to support superior performance to 2D (planar) representations, but only for more integrative questions. Animated motion provided no benefits. In Experiment 2, stereoptic views of a 3D display were also found to support performance, particularly for integrative questions, but the ability to rotate the data space (motion parallax) and the presence of a mesh surface connecting the points did not. The posttests revealed some evidence that 3D representations improved the ability to visualize the surface, but neither 3D renderings nor stereopsis led to a better abstract representation of the data.