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A compact hardware-software complex for measuring the electrical and mechanical parameters of a liquid based on a piezoelectric resonator with a lateral exciting electric field has been developed. It is shown that the modulus of the electrical impedance of the resonator near the resonant frequency decreases monotonically with an increase in conductivity of the contacting liquid. The obtained dependences can be used as calibration curves for measuring conductivity in the range of 100-10,000 µS/cm. A method for the simultaneous determination of the modulus of elasticity, viscosity, and permittivity of a liquid using the electromechanical equivalent scheme was developed and tested on a mixture "water - glycerol" with the different glycerol content.
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A microbial test-system for real-time determination of low/residual concentrations of kanamycin in a liquid without the need for special labels is presented. The main element of the system was a piezoelectric resonator excited by a lateral electric field based on an X-cut lithium niobate plate 0.5 mm thick with two rectangular electrodes on one side. On the other side of the resonator, there was a 1.5 ml liquid container. As a sensory element we used Escherichia coli B-878 microbial cells, which are sensitive to kanamycin. For measurement 1 ml of this cells suspension was placed in a liquid container and then the test liquid in the amount of 2 µl containing kanamycin was added. The change in the real part of the electrical impedance of the resonator before and after the test liquid addition was used as an analytical signal which indicated the presence of kanamycin. The lower limit of determination of kanamycin turned out to be 1.0 µg/ml with an analysis time of 10 min. The test-system allows to detect kanamycin in the presence of such antibiotic as ampicillin and polymixin.
Assuntos
Técnicas Biossensoriais/instrumentação , Técnicas Eletroquímicas/instrumentação , Canamicina/química , Antibacterianos/química , Impedância Elétrica , Escherichia coli/efeitos dos fármacos , Nióbio/química , Óxidos/química , SuspensõesRESUMO
The influence of the conductivity of a liquid contacting with a piezoelectric resonator with a lateral electric field based on a PZT piezoceramic plate was experimentally and theoretically investigated. In this resonator the shear component of the mechanical displacement, which does not lead to radiation losses upon contact with the liquid, was prevalent. The frequency dependences of the real and imaginary parts of the electrical impedance of the resonator in the frequency range 50-300 kHz showed the presence of three resonances at frequencies of 68.7, 97.8 and 264 kHz with the values of the electro-mechanical coupling coefficient of 12.2%, 14.7% and 6.5%, respectively. The quality factor of each resonance in contact with the liquid turned out to be significantly higher than the quality factor of the resonator with a longitudinal acoustic wave based on lithium niobate. The dependences of the maximum value of the real part of the electrical impedance of such a resonator on the conductivity of the liquid for each resonance were obtained. This experimental data turned out to be in a good agreement with theoretical results obtained by using the method of equivalent circuit.
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For the first time, a rapid method was proposed to determine the susceptibility of Escherichia coli cells to antibiotics by the example of ampicillin by using a biological sensor based on a slot mode in an acoustic delay line. It has been established that an indicator of the antibiotic activity to microbial cells is the difference between the recorded sensor's signal before and after exposure cells with antibiotic. The depth and frequency of the peaks of resonant absorption in the frequency dependence of the insertion loss of sensor varied after adding an antibiotic with different concentrations to the microbial cells. By using the acoustic sensor based on slot-mode a criterion of E. coli sensitivity to ampicillin was established. The advantages of this method are the ability to carry out the analysis directly in the liquid, the short analysis time (within 10-15 min), and the possibility to reusable sensor.
Assuntos
Antibacterianos/farmacologia , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Escherichia coli/efeitos dos fármacos , Testes de Sensibilidade Microbiana/instrumentação , Testes de Sensibilidade Microbiana/métodos , Acústica/instrumentaçãoRESUMO
Theoretical and experimental studies of the influence of a thin metal film on the characteristics of a piezoelectric resonator with a lateral electric field showed the possibility of creating a micro-displacement meter in the range of 10-300⯵m. For the experiments, two resonators based on the plates of PZT piezoceramics with a thickness of 3.56 and 4.46â¯mm with resonant frequencies of ~96 and ~260â¯kHz for both resonators were used. It has been experimentally established that in both cases, with an increase in the width of the gap between the free side of the piezoelectric resonator and thin aluminum film, the frequency of the parallel resonance and maximum value of the real part of the electrical impedance increase and reach saturation. Besides, it has been shown that the relative change of these values with a change in the width of the gap in the range 10-300⯵m increases with decreasing the thickness of the resonator. In this case, the frequency of the series resonance practically does not change. It has been also established that the sensitivity of the resonator to the presence of a conducting film at the resonant frequency of ~96â¯kHz is significantly higher in comparison with the resonant frequency of ~260â¯kHz. The experimental results are in the qualitative agreement with the theoretical data.
RESUMO
The paper describes a new biological sensor which represents a resonator based on a segment of a rectangular waveguide of 8â¯GHz band with shear dimensions of 28.5â¯×â¯12.6â¯mm2. On one side, the resonator is bounded by a metallic short-circuited wall; on the other side, it is bounded by a lithium niobate plate with a porous polystyrene film. This film, applied by centrifugation and modified in high-frequency discharge plasma in argon, was used to immobilize cells of Escherichia coli K-12. This resonator was connected through a coaxial-waveguide adapter to the S parameter meter, by means of which the reflection coefficient S11 in the plane of the lithium niobate plate was measured. The addition of an aqueous solution of ampicillin at 4-50⯵g/ml to immobilized cells led to a significant change in the reflection coefficient of S11 from -â¯10.15â¯dB to -â¯15.09â¯dB. At the same time, the resonance frequency changed insignificantly within the range 8.06-8.068â¯GHz. The optimal time for modifying the polystyrene film for obtaining the required porosity and the optimal time for the immobilization of the bacterial cells were determined. The immobilized cells retained their activity for 4 months at a temperature of 4⯰C. The study showed the promise of such a biosensor to determine ß-lactam antibiotics in aqueous solutions by using ampicillin as an example. The limit of detection of the developed biosensor with respect to ampicillin was established (4⯵g/ml).
Assuntos
Ampicilina/isolamento & purificação , Técnicas Biossensoriais , Escherichia coli K12/química , Ampicilina/química , Micro-Ondas , Nióbio/química , Óxidos/química , Temperatura , Água/químicaRESUMO
In this paper, we investigated theoretically and experimentally the effect of the conductivity of the film located in the immediate vicinity of the free side of a piezoelectric resonator based on the PZT ceramics on its characteristics. It is shown that with increasing the conductivity of the film at first the parallel resonant frequency does not change, then it decreases in a certain interval and after that remains practically constant. With increasing the width of the gap between the film and the resonator, the resonant frequency increases and reaches the saturation. At that the degree of the total frequency change decreases with decreasing the conductivity of the film. We also investigated the dependence of the maximum value of the real part of the electrical impedance on the film conductivity at the different values of the width of the gap between the film and the resonator. The dependences of the maximum value of the real part of the electrical impedance on the width of the gap between the film and the resonator are also obtained for different values of the film conductivity. A comparison of the theoretical and experimental data showed their good qualitative agreement.
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The results of the study of the influence of the conductivity of a thin film located with a certain gap above the acoustic delay line on the characteristics of propagating acoustic wave with a quasi-shear-horizontal polarization (SH0) are presented. The delay line was made of Y-X lithium niobate plate with a thickness of 200⯵m containing two interdigital transducers for exciting and receiving SH0 wave. The operating frequency range of the delay line was equal to 2.6-3.8â¯MHz. The 1â¯mm thick glass plate with a film of the given conductivity applied on one plate's side was placed above this delay line with the some air gap. The change in the phase of output signal of the delay line was measured at the various values of the width of this gap. As films with the various values of the conductivity, tin dioxide films deposited on a glass plate by high-frequency reactive magnetron sputtering in the oxygen-argon mixture were used. It has been shown that the velocity of SH0 wave propagating in the delay line under investigation depends on the film conductivity and the width of the gap between the delay line and conductive film. The dependences of the change in the SH0 wave velocity on a width of the gap between the surface of the delay line and conductive film were constructed, as well as on the film conductivity. The comparison of the experimental results with the theoretical data showed their good agreement.
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The possibility of the registration of the interaction of the cells Azospirillum lipoferum Sp59b with the specific antibodies directly in the conducting suspensions by using an acoustic sensor was shown. The main element of the sensor is a piezoelectric resonator with a lateral electric field. The analysis is based on a comparison of the resonator's electrical impedance before and after the specific biological interaction between the cells and antibodies. By using this sensor one can detect and identify the bacterial cells directly in the buffer solution with the conductivity between 2.4 and 20⯵S/cm. The minimum detectable concentration of the bacterial cells turned out to be â¼103â¯cells/ml and for a short time (less than 10â¯min). Also the possibility of the detection of the cells in the presence of the extraneous microflora was shown. The results provide the opportunities for the development of a new class of the methods for the analysis of the microbial cells in real-time directly in the buffer solution.
Assuntos
Anticorpos Antibacterianos , Azospirillum lipoferum/imunologia , Técnicas Biossensoriais/métodos , Acústica , Especificidade de Anticorpos , Reações Antígeno-Anticorpo , Azospirillum lipoferum/ultraestrutura , Soluções Tampão , Sistemas Computacionais , Impedância Elétrica , Lipopolissacarídeos/imunologiaRESUMO
The influence of the liquid with the different values of conductivity, permittivity, and viscosity on the characteristics of the slot mode in the structure consisting of delay line with propagating shear - horizontal acoustic wave of zero order (SH0) and upper piezoelectric plate separated by the air gap was experimentally investigated. The delay line made of the Y-X lithium niobate plate 0.2mm thick contained two interdigital transducers for the excitation and reception of acoustic wave in the frequency range of 2.6-3.8MHz. The upper plate represented the 0.5mm thick plate of Z-X lithium niobate. The excitation of the slot mode led to the appearance of the sharp resonant peaks on the frequency dependencies of insertion loss and phase of the output signal. It has been found that the depth and frequency of these peaks depend on the parameters of liquid contacting with the upper plate. The possibility of the use of the slot mode in the pointed structure for the identification of liquids with the different values of the conductivity, viscosity, and permittivity was shown. The qualitative explanations of the obtained experimental results are presented.
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The effect of a thin layer with the finite surface conductivity located near the lateral electric field excited resonator on its characteristics is studied theoretically and experimentally. It has been shown that for the fixed distance between the free side of the resonator and conducting layer with increasing the surface conductivity of the layer the resonant frequency of the parallel resonance remains initially practically constant, then sharply decreases in a certain range and then insignificantly changes. For the fixed value of the layer conductivity the parallel resonant frequency increases at the increase in the gap between the resonator and layer and then achieves the saturation. The maximum change in the frequency of the parallel resonance corresponds to a zero gap when the layer conductivity varies over the wide range is equal to â¼1%. The frequency of the series resonance decreases only by â¼0.08% due to the change in the layer conductivity. The obtained results may be useful for the development of the gas sensors based on the lateral electric field excited piezoelectric resonator conjugated to the gas sensitive film, the conductivity of which changes in the presence of the given gas.
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The influence of the width of the gap between the free side of the piezoelectric lateral electric field excited resonator and the metal film placed on the dielectric plate on the frequencies of the parallel and series resonances was experimentally and theoretically studied. The measurements were carried out in the temperature range of 14-45⯰C. It has been shown that the change of the gap width from 0 up to 3.5â¯mm leads to the change of the parallel resonant frequency on 1.3% at the constant temperature. At the same conditions the change of the series resonant frequency does not exceed 0.07%. Theoretical analysis quantitatively confirmed the experimental dependencies of the aforementioned frequencies on the gap width at the room temperature. At that the maximum difference between the theoretical and experimental values of the parallel and series resonant frequencies in all cases does not exceed 1.2%. The obtained results may be used as the basis for the development of the sensors for the measurement of the displacement in the interval of 0.2-2â¯mm in the temperature range of 15-45⯰C.
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The acoustic biological sensor for the analysis of the bacterial cells in conducting suspension was developed. The sensor represented the two channel delay line based on the piezoelectric plate of Y-X lithium niobate thick of 0.2mm. Two pairs of the interdigital transducers (IDT) for the excitation and reception of shear horizontal acoustic wave of zero order (SH0) in each channel were deposited by the method of photolithography. One channel of the delay line was electrically shorted by the deposition of thin aluminum film between IDTs. The second channel remained as electrically open. The liquid container with the volume of 5ml was fixed on the plate surface between IDTs by the glue, which did not cause the additional insertion loss. For the first time the influence of the conductivity of the cell suspension on the registration of the specific and nonspecific interactions of the bacterial cells with phage-antibodies (phage-Abs) was studied by means of the developed sensor. The dependencies of the change in insertion loss and phase of the output signal on the conductivity of the buffer solution at specific/nonspecific interactions for the electrically open and shorted channels of the delay line were obtained. It was shown that the sensor successfully registered the interactions of microbial cells with phage-Abs in the range of the conductivity of 2-20 µS/cm on the model samples A. brasilense Sp245 - specific phage-Abs. The sensor in the time regime of the operation fast reacted on the specific/nonspecific interaction and the time of the stabilization of the output parameters did not exceed 10min.
Assuntos
Anticorpos , Azospirillum brasilense , Bacteriófagos , Técnicas Biossensoriais , Escherichia coli K12 , Nióbio , Óxidos , SomRESUMO
The electro-acoustical sensor for the real-time identification of the bacteriophage FAl-SR65 is described. The polyclonal antibodies specific to the bacteriophage FAl-SR65 were for the first time obtained, and the possibility of their application to the bacteriophage detection by using the electro-acoustical method of analysis was also studied for the first time. The electro-acoustical sensor was based on the lateral electric field excited piezoelectric resonator. The frequency dependences of the real and imaginary parts of the electrical impedance of such a resonator, loaded with the virus-antibody suspension, significantly differed from those measured for the same resonator with the same virus suspension but without antibodies (control). The limit for the reliable bacteriophage detection during their interaction with the antibodies (sensitivity level) was determined to be 106 phages ml-1. The obtained results demonstrate the possibility to record the bacteriophage-antibody interactions and can be used as the basis for the development of the biological sensor for the quantitative virus detection.
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Acústica , Bacteriófagos/isolamento & purificação , Eletroquímica/instrumentação , Fatores de TempoRESUMO
The possibility of using the acoustic sensor on the basis of a two-channel delay line for rapid analysis of bacterial cells in the conductive suspensions was investigated. The dependencies of change in phase and insertion loss of output signal of the sensor on conductivity of buffer solution with various concentrations of cells due to a specific interaction "bacterial cells - mini-antibodies" for electrically open and electrically shorted channels of delay line were measured. It has been found that these changes have the most values for the electrically open channel. It has been also shown that the sensor rapidly responds to the specific interaction and the time stabilization of the phase and insertion loss of output signal is less than 10min.
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Acústica , Anticorpos Antibacterianos/isolamento & purificação , Técnicas Biossensoriais/instrumentação , Azospirillum brasilense , Eletricidade , Escherichia coli , Limite de Detecção , SuspensõesRESUMO
It has been shown that by using piezoelectric lateral electric field excited resonators based on X - cut LiNbO3, one can determine the octane number of gasoline. The measured dependence of gasoline permittivity on its octane number has shown that there is an ambiguous connection between pointed parameters. We have demonstrated both theoretically and experimentally that the value of the real part of the electrical impedance on the frequency of parallel resonance uniquely associates with the octane number of gasoline contacting the free side of the resonator. At that the frequency of parallel resonance does not depend on permittivity/octane number of gasoline. An example of determination of the octane number of a mixture of two different samples of gasoline is given.
RESUMO
The novel method of suppression of parasitic oscillations in lateral electric field excited piezoelectric resonator is suggested. Traditionally such resonator represents the piezoelectric plate with two electrodes on one side of the plate. The crystallographic orientation of the plate is selected so that the tangential components of electric field excite bulk acoustic wave with given polarization travelling along the normal to the plate sides. However at that the normal components of field excite the parasitic Lamb waves and bulk waves of other polarization which deteriorate the resonant properties of the resonator. In this work we suggest to separate the source of the HF electric field and resounded piezoelectric plate by air gap. In this case the tangential components of the field in piezoelectric plate do not practically weaken but normal components significantly decrease. This method is realized on the composite resonator having the structure "glass plate with rectangular electrodes - air gap - plate of 128 Y-X lithium niobate." It has been shown that there exist the optimal value of the width gap which ensure the good quality of series and parallel resonances in frequency range 3-4MHz with record values of Q-factor of â¼15,000 in both cases.
RESUMO
It has been demonstrated that electroacoustic analysis with polyclonal antibodies can be used for bacteriophage detection. The frequency dependences of the real and imaginary parts of electrical impedance of a resonator with a viral suspension with antibodies were shown to be essentially different from the dependences of a resonator with control viral suspension without antibodies. It was shown that ΦAl-Sp59b bacteriophages were detected with the use of antibodies in the presence of foreign virus particles. The ΦAl-Sp59b bacteriophage content in the analyzed suspension was ~1010106 phages/mL; the time of analysis was no more than 5 min. The optimally informative parameter for obtaining reliable information was the change in the real or imaginary part of electrical impedance at a fixed frequency near the resonance upon the addition of specific antibodies to the analyzed suspension. It was demonstrated that the interaction between bacteriophages and antibodies can be recorded, offering good prospects for the development of a biological sensor for liquid-phase identification and virus detection.
Assuntos
Anticorpos Antivirais/química , Azospirillum/virologia , Bacteriófagos/química , Campos Eletromagnéticos , Animais , Anticorpos Antivirais/imunologia , Azospirillum/imunologia , Bacteriófagos/imunologia , Técnicas Imunológicas/métodos , CoelhosRESUMO
The changes in the electro-acoustic parameters of cell suspension due to the interaction of cells with bacteriophages both in a pure. culture and in the presence of extraneous microflora were investigated. It has been found that the specific changes in the electroacoustic parameters of cell suspension under the action of bacteriophage occur only in microbial cells which are sensitive to the bacteriophage studied. It has been established that a sensor unit allows of distinguishing a situation when the bacterial cells are infected with specific bacteriophages of the control experiments and a situation with no introduction of infection. An approximate criterion of the presence of specific interactions of bacteriophages and cells in suspension was developed. In accordance with this criterion the change in electrical impedance of the sensor unit must not be less than - 1%. In control experiments a standard microbiological technique, plating the cells infected with bacteriophages on solid nutrient medium, was used. For the first time the possibility of using the method of electroacoustic analysis for determination of a spectrum of lytic activity of bacteriophages was shown. The results obtained may be used for development of a new express method for determining the sensitivity to bacteriophages of the microbial cells.
Assuntos
Acinetobacter calcoaceticus/virologia , Azospirillum brasilense/virologia , Bacteriófago M13/fisiologia , Escherichia coli/virologia , Lisogenia/fisiologia , Pseudomonas putida/virologia , Acinetobacter calcoaceticus/imunologia , Acústica/instrumentação , Antibiose , Azospirillum brasilense/imunologia , Impedância Elétrica , Escherichia coli/imunologia , Especificidade de Hospedeiro , Pseudomonas putida/imunologiaRESUMO
An array containing two resonators placed on X-cut lithium niobate plate has been experimentally investigated. The resonator's lateral electric field was directed along the Y-crystallographic axis. It has been shown that stable resonance exists for a longitudinal acoustic wave propagating along the X-axis in the area between the electrodes. A layer of special damping coating was deposited around the resonators and on the part of electrodes to suppress parasitic oscillations induced mainly by Lamb waves. Frequency dependences of the real and imaginary parts of electric impedance/admittance were measured for every resonator to find resonant frequency and Q-factor with series and parallel resonances. The optimal values of width of electrode coating for every resonator were revealed which provide good resonance quality. The measurements of parameter S12, which characterizes a degree of acoustical coupling between the resonators, have shown its value to be higher than 50dB in the absolute value in all the cases considered. This means that the resonators under study are entirely acoustically decoupled. Thus it has been demonstrated that the damping layer not only provides a sufficiently good quality of every resonator's resonance, but it also assures their entire acoustical decoupling.