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1.
Wei Sheng Yan Jiu ; 49(5): 705-710, 2020 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-33070809

RESUMO

OBJECTIVE: To explore the effect of pre-pregnancy weight and gestational weight gain in Chongqing City on birth weight of newborns. METHODS: Pregnant women were volunteered for the cohort study in 6 Maternal and Child Centers in Chongqing from January 2016 to June 2017, who were planning to be pregnant in latest 3 months. The basic information included height and weight of prepregnancy, first trimester, mid-trimester and third trimester of pregnancy, and pregnancy outcome were collected. ANOVA, chi square test and multi factor unconditional logistic regression model were used to analyze the data. RESULTS: In pre-pregnancy the major BMI of women were <18. 5(46. 74%, 1119/2394) and 18. 5≤BMI≤29. 99(47. 12%, 1128/2394), in first trimester of pregnancy, the figures were <18. 5(34. 46%, 825/2394) and 18. 5≤BMI≤29. 99(57. 77%, 1383/2394). The distribution BMI in above stages were statistically different(χ~2=74. 95, P<0. 01). The incidence of the low birth weight and macrosomia were 4. 51%(108/2394) and 6. 89%(165/2394). The average of neonatal birth weight, the low birth weight and macrosomia were statistically different(F=24. 18, P<0. 01) and(χ~2=66. 44, P<0. 01) comparisons among all prepregnancy BMI groups. The average birth weight of newborns, the low birth weight of the newborn and macrosomia were statistically different(F=11. 27, P<0. 01), and(χ~2=89. 53, P<0. 01) comparisons among all IOM groups of three stages of pregnancy. Low weight in pregnancy is the risk factor for low birth weight infants(RR=1. 90(95%CI 1. 27-2. 86)), while excessive gestational weight gain is the protective factor(RR=0. 66(95%CI 0. 46-0. 96)). Prepregnancy obesity(RR=3. 06(95%CI 1. 77-5. 31)) and excessive weight gain during pregnancy(RR=3. 60(95%CI 2. 48-5. 22)) were the risk factors for macrosomia. The above two factors, prepregnancy obesity and excessive weight gain during pregnancy interacted multiply. CONCLUSION: The figures of pre-pregnancy BMI are not equal to the BMI in first trimester of pregnancy. Low weight in pre-pregnancy is the risk factor for low birth weight infants, while excessive weight gain during pregnancy is the protective factor. Prepregnancy obesity and excessive weight gain during pregnancy are the risk factors for macrosomia. Prepregnancy obesity and excessive weight gain during pregnancy interacted multiply.


Assuntos
Ganho de Peso na Gestação , Peso ao Nascer , Índice de Massa Corporal , Criança , Estudos de Coortes , Feminino , Humanos , Recém-Nascido , Gravidez , Estudos Prospectivos
2.
Hortic Res ; 7: 161, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33082968

RESUMO

The carotenoid isomerase gene (BoaCRTISO) of Chinese kale was targeted and edited using the CRISPR/Cas9 system in the present study. The results showed a high mutation rate (81.25%), and 13 crtiso mutants were obtained. Only two types of mutations, insertions and replacements, were found. Both the total and individual carotenoid and chlorophyll concentrations of the biallelic and homozygous mutants were reduced, and the total levels declined by 11.89-36.33%. The color of the biallelic and homozygous mutants changed from green to yellow, likely reflecting a reduction in the color-masking effect of chlorophyll on carotenoids. The expression levels of most carotenoid and chlorophyll biosynthesis-related genes, including CRTISO, were notably lower in the mutants than in the WT plants. In addition, the functional differences between members of this gene family were discussed. In summary, these findings indicate that CRISPR/Cas9 is a promising technique for the quality improvement of Chinese kale and other Brassica vegetables.

3.
PeerJ ; 8: e9932, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33083111

RESUMO

The mitochondrial genomes (mitogenomes) of scale insects are less known in comparison to other insects, which hinders the phylogenetic and evolutionary studies of Coccoidea and higher taxa. Herein, the complete mitogenomes of Unaspis yanonensis, Planococcus citri and Ceroplastes rubens were sequenced for Coccoidea. The 15,220-bp long mitogenome of U. yanonensis contained the typical set of 37 genes including 13 PCGs, 22 tRNA genes and two rRNA genes; the 15,549-bp long mitogenome of P. citri lacked the tRNA gene trnV; the 15,387-bp long mitogenome of C. rubens exhibited several shortened PCGs and lacked five tRNA genes. The mitochondrial gene arrangement of the three mitogenomes was different from other scale insects and Drosophila yakuba. Most PCGs used standard ATN (ATA, ATT, ATC and ATG) start codons and complete TAN (TAA or TAG) termination codons. The ND4L had the highest evolutionary rate but COX1 and CYTB were the lowest. Most tRNA genes had cloverleaf secondary structures, whereas the reduction of dihydrouridine (DHU) arms and TψC arms were detected. Tandem repeats, stem-loop (SL) structures and poly-[TA]n stretch were found in the control regions (CRs) of the three mitogenomes. The phylogenetic analyses using Bayesian inference (BI) and maximum likelihood methods (ML) showed identical results, both supporting the inner relationship of Coccoidea as Coccidae + (Pseudococcidae + Diaspididae).

4.
Zootaxa ; 4759(4): zootaxa.4759.4.3, 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33056893

RESUMO

Based on morphological, molecular, and ecological evidences, a fourth species of cave-dwelling shrimp Macrobrachium tenuipes sp. nov. is described from Guangxi Zhuang Autonomous Region, southwestern China. This new species with smooth carapace and the extremely slender pereiopods, can be separated from other congeners by the shape of rostrum; the segmental ratios, the arrangement of teeth on the cutting edge of the fingers of second pereiopod; the longer and narrower scaphocerite; and the longer uropodal diaeresis spine. The new taxon is genetically distinct from 18 other species of Macrobrachium for which COI and 18S rRNA sequences are available. The data observed on the habitat and ecology will contribute to the conservation for M. tenuipes sp. nov.

5.
Hereditas ; 157(1): 41, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33054858

RESUMO

BACKGROUND: Cervical cancer (CC) is the third most common gynecological malignancy around the world. Cisplatin is an effective drug, but cisplatin resistance is a vital factor limiting the clinical usage of cisplatin. Enhancer of mRNA decapping protein 4 (EDC4) is a known regulator of mRNA decapping, which was related with genome stability and sensitivity of drugs. This research was to investigate the mechanism of EDC4 on cisplatin resistance in CC. Two human cervical cancer cell lines, HeLa and SiHa, were used to investigate the role of EDC4 on cisplatin resistance in vitro. The knockdown or overexpression of EDC4 or replication protein A (RPA) in HeLa or SiHa cells was performed by transfection. Cell viability was analyzed by MTT assay. The growth of cancer cells was evaluated by colony formation assay. DNA damage was measured by γH2AX (a sensitive DNA damage response marker) immunofluorescent staining. The binding of EDC4 and RPA was analyzed by immunoprecipitation. RESULTS: EDC4 knockdown in cervical cancer cells (HeLa and SiHa) enhanced cisplatin sensitivity and cisplatin induced cell growth inhibition and DNA damage. EDC4 overexpression reduced DNA damage caused by cisplatin and enhanced cell growth of cervical cancer cells. EDC4 could interact with RPA and promote RPA phosphorylation. RPA knockdown reversed the inhibitory effect of EDC4 on cisplatin-induced DNA damage. CONCLUSION: The present results indicated that EDC4 is responsible for the cisplatin resistance partly through interacting with RPA in cervical cancer by alleviating DNA damage. This study indicated that EDC4 or RPA may be novel targets to combat chemotherapy resistance in cervical cancer.

6.
Sci Total Environ ; : 142841, 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33077217

RESUMO

Biochar and woody peat have been recognized as an additive to reduce carbon and nitrogen loss during composting. Yet little is known about their influences on the transformation of phosphorus (P) fractions in composting. This study investigated the quantitative and qualitative changes in different P forms during composting with adding biochar or woody peat using sequential extraction and P K-edge X-ray absorption near-edge structure (XANES). The results showed that compost products from the treatment with adding woody peat had a higher HA/FA (the ratio of humic acid to fulvic acid) compared to biochar treatment and the control, suggesting that the addition of woody peat might benefit the humification process of composting. Sequential extraction and XANES illustrated that adding biochar or woody peat limited the P availability. Biochar increased the proportion of Pi and woody peat decreased the conversion from Po to Pi compared to the control. Structural equation modeling and redundancy analysis suggested that biochar improved the refractory P based on the indirect effects of NH4+-N by regulating microbial community, while woody peat was beneficial for Po accumulation by affecting humic acid. Taken together, this research provides basis for regulating the nutrient level of carbon, nitrogen, and phosphorus in composts and reducing environmental risks.

7.
Org Biomol Chem ; 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33078807

RESUMO

A copper-promoted oxidative cyanomethylation/ring-expansion of vinylcyclopropanes with α-C(sp3)-H bonds in alkyl nitriles is established for the generation of 1-cyanoethylated 3,4-dihydronaphthalenes. This cyanomethylation/ring-expansion involves a radical pathway and proceeds via cyanomethyl radical formation, radical addition and ring-expansion. This ring-expansion strategy offers a highly atom-economical route for the construction of nitrile-containing 3,4-dihydronaphthalenes, which can be transformed into other useful products under simple conditions.

8.
Anal Biochem ; 609: 113974, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33010205

RESUMO

Antibody-based therapeutics targeting membrane proteins have evolved as a major modality for the treatment of cancer, inflammation and autoimmune diseases. There are numerous challenges, ranging from desired epitope expression to reliable binding/functional assays which are associated with developing antibodies for this target class. Specifically, having a robust methodology for characterizing antibody interaction with a membrane protein target is essential for providing guidance on dosing, potency and thus expected efficacy. Fluorescence-activated cell sorting (FACS) has been commonly used to characterize antibodies binding to membrane protein targets. FACS provides information about the antibody-receptor complex (antibody bound to cells) and the apparent equilibrium dissociation constant (KD') is elucidated by fitting the antibody-receptor binding isotherm as a function of total antibody concentration to a nonlinear regression model. Conversely, Kinetic Exclusion Assay (KinExA) has been used to measure solution-based equilibrium dissociation constant (KD) of antibodies. Here, KD is determined by measuring the free antibody concentration at equilibrium in a series of solutions in which the antibody is at constant concentration and the receptor (either in the membrane or the cell) is titrated. We measured the binding affinity of the anti-CD20 antibody, Rituximab, using both FACS and KinExA. There was ~25-fold difference in the binding affinity measured by these two techniques. We have explored this discrepancy through additional experiments around the mathematical framework involved in the analysis of these two different binding assays. Finally, our study concluded that KinExA enables accurate measurement of the KD for strong protein-protein interactions (sub-nanomolar values) compared to FACS.

9.
Org Lett ; 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33049136

RESUMO

Research on perfluoroalkyl ether carboxylic acids (PFECAs) as alternatives for perfluoroalkyl substances continues with the goal of protecting the environment. However, very little is known about the utilization of decomposition products of PFECAs. We report herein a new series of deoxyfluorination reagents featuring OCF2 functional groups derived from certain PFECAs. Alkyl fluorides were generated from various alcohols in ≤97% yield by these novel reagents. The mechanistic experiment verified in situ generation of carbonic difluoride (COF2).

10.
Expert Rev Clin Immunol ; : 1-12, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33012198

RESUMO

OBJECTIVES: This meta-analysis was performed to investigate the associations between single-nucleotide polymorphisms (SNPs) in the TGF- ß and Smad3 genes and arthritis. METHODS: A meta-analysis was performed in STATA 14.0, with publication bias and meta-regression analysis. All types of arthritis were included, and subgroup analyses were performed to interpret variations among different types of arthritis. RESULTS: Twenty-two qualified studieswere selected to analyze the pooled accuracy, and 4 SNP sites were involved. The analysis of the TGFB1 SNP rs1800470 showed an association with arthritis in allelic (P = 0.011), homozygous (P = 0.034) and recessive (P = 0.021) genetic models. The analysis of the TGFB1 SNP rs1800471 demonstrated a close association with rheumatoid arthritis (RA) in homozygous (P = 0.000, 95%) and recessive (P = 0.008) models. The analysis of the SMAD3 SNP rs12901499 revealed a close association with osteoarthritis (OA) in the allelic (P = 0.001) model. CONCLUSION: This research showed that genetic variants of the TGF-ß pathway impact arthritis. The polymorphisms rs1800470, rs1800471 and rs12901499 were correlated with a higher prevalence of arthritis.

11.
Chem Commun (Camb) ; 56(78): 11685-11688, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33000809

RESUMO

1,2-Aryl migration of α,α-diaryl tertiary azides was achieved by using the catalytic system of FeCl2/N-heterocyclic carbene (NHC) SIPr·HCl. The reaction generated aniline products in good yields after one-pot reduction of the migration-resultant imines.

12.
Future Microbiol ; 15: 1265-1276, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33026882

RESUMO

MRSA is a super drug-resistant bacterium. Developing new drug or therapeutic strategies against MRSA is urgently needed. Increasing evidence has shown that herbal extracts and antibiotics can have synergistic effects against MRSA. This review focuses on commonly used antibiotics combined with herbal extracts against MRSA and the corresponding mechanisms. Through systematic analysis, we found that herbal extracts combined with antibiotics, such as ß-lactams, quinolones, aminoglycosides, tetracyclines and glycopeptides, could greatly enhance the antibacterial effects of the antibiotics, reduce the dosage and toxic side effects, and reverse MRSA resistance. Therefore, we conclude that herbal extracts combined with antibiotics may be a promising strategy to combat MRSA. This review provides a novel idea for overcoming antibiotic resistance.

13.
Nucleic Acids Res ; 2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33010154

RESUMO

High-throughput genetic screening based on CRISPR/Cas9 or RNA-interference (RNAi) enables the exploration of genes associated with the phenotype of interest on a large scale. The rapid accumulation of public available genetic screening data provides a wealth of knowledge about genotype-to-phenotype relationships and a valuable resource for the systematic analysis of gene functions. Here we present CRISP-view, a comprehensive database of CRISPR/Cas9 and RNAi screening datasets that span multiple phenotypes, including in vitro and in vivo cell proliferation and viability, response to cancer immunotherapy, virus response, protein expression, etc. By 22 September 2020, CRISP-view has collected 10 321 human samples and 825 mouse samples from 167 papers. All the datasets have been curated, annotated, and processed by a standard MAGeCK-VISPR analysis pipeline with quality control (QC) metrics. We also developed a user-friendly webserver to visualize, explore, and search these datasets. The webserver is freely available at http://crispview.weililab.org.

14.
Int J Mol Sci ; 21(19)2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33023038

RESUMO

Glucose-6-phosphate dehydrogenase (G6PDH) plays an important role in plant stress responses. Here, five FaG6PDH sequences were obtained in strawberry, designated as FaG6PDH-CY, FaG6PDH-P1, FaG6PDH-P1.1, FaG6PDH-P2 and FaG6PDH-P0, which were divided into cytosolic (CY) and plastidic (P) isoforms based on the bioinformatic analysis. The respective FaG6PDH genes had distinct expression patterns in all tissues and at different stages of fruit development. Notably, FaG6PDH-CY was the most highly expressed gene among five FaG6PDH members, indicating it encoded the major G6PDH isoform throughout the plant. FaG6PDH positively regulated cold tolerance in strawberry. Inhibition of its activity gave rise to greater cold-induced injury in plant. The FaG6PDH-CY transcript had a significant increase under cold stress, similar to the G6PDH enzyme activity, suggesting a principal participant in response to cold stress. Further study showed that the low-temperature responsiveness (LTR) element in FaG6PDH-CY promoter can promote the gene expression when plant encountered cold stimuli. Besides, FaG6PDH-CY was involved in regulating cold-induced activation of antioxidant enzyme genes (FaSOD, FaCAT, FaAPX and FaGR) and RBOH-dependent ROS generation. The elevated FaG6PDH-CY enhanced ROS-scavenging capability of antioxidant enzymes to suppress ROS excessive accumulation and relieved the oxidative damage, eventually improving the strawberry resistance to cold stress.

15.
Oral Dis ; 2020 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-32881142

RESUMO

OBJECTIVE: To assess the association of preoperative lymphocyte-to-monocyte ratio (LMR) and overall survival (OS) in oral cancer patients and develop a dynamic nomogram for individualized survival prediction. METHOD: The prognostic value of LMR was evaluated in a large-scale cohort with 651 postoperative oral cancer patients between January 2010 and December 2017. Propensity score-matched (PSM) analysis and inverse probability of treatment weighting (IPTW) analysis were performed to further verify the prognostic value of LMR. A dynamic nomogram was then developed based on the LMR and clinicopathological features, and its predictive performance and clinical utility were evaluated. RESULTS: A high LMR was significantly associated with better OS of oral cancer patients (HR=0.65; 95%CI=0.44-0.98). The similar association was also observed in the PSM and IPTW analyses. Moreover, compared to TNM staging system, the dynamic nomogram based on the LMR exhibited more excellent predictive performance (0.72 vs. 0.64, p< 0.001), with calibration curves (1000 bootstrap resamples) suggesting good match between the actual and predicted probabilities. Decision curve analyses (DCAs) showed a more significant positive net benefit in the practical ranges of threshold probabilities using the dynamic nomogram. CONCLUSION: Preoperative LMR may serve as an easily accessible and non-invasive prognostic biomarker for predicting the prognosis of oral cancer patients. A dynamic nomogram based on the LMR may show more convenience in survival prediction for patients with oral cancer. Further future studies are warranted to confirm our findings.

16.
Artigo em Inglês | MEDLINE | ID: mdl-32990397

RESUMO

Transition metal-catalyzed decarbonylation offers a distinct synthetic strategy for new chemical bond formation. However, the π-backbonding between CO π* orbitals and metal center d-orbitals impedes ligand dissociation to regenerate catalyst under mild condition. Herein, we developed a visible light-induced rhodium catalysis for decarbonylative coupling of imides with alkynes under ambient conditions. Initial mechanistic studies suggest that the rhodium complex simultaneously serves as the catalytic center and photosensitizer for decarbonylation. This visible light-promoted catalytic decarbonylation strategy offers new opportunities for reviewing old transformations with ligand dissociation as a rate determining step.

18.
J Hum Genet ; 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32968204

RESUMO

To explore whether DNA methylation of the ATP-binding cassette G1 (ABCG1) gene and its dynamic change are associated with incident type 2 diabetes mellitus (T2DM). We conducted a nested case-control study with 286 pairs of T2DM cases and matched controls nested in the Rural Chinese Cohort Study. Conditional logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for incident T2DM risk according to ABCG1 methylation level at baseline and its dynamic change at follow-up examination. Spearman's rank correlation coefficients were used to analyze the association between ABCG1 methylation and its possible risk factors in the control group. We found that T2DM risk increased by 16% (OR = 1.16, 95% CI = 1.02-1.31) with each 1% increase in DNA methylation levels of the ABCG1 loci CpG13 and CpG14. DNA methylation change of the ABCG1 locus CpG15 during the 6-year follow-up was associated with increased T2DM risk: T2DM risk increased by 78% in the upper tertile group (methylation gain ≥5%) versus lower tertile group (methylation gain <1%) (OR = 1.78, 95% CI = 1.01-3.15). Furthermore, body mass index was positively correlated with the DNA methylation level of the ABCG1 loci CpG13, CpG14 and CpG15. In conclusion, DNA methylation levels of the ABCG1 loci CpG13 and CpG14 and the methylation gain of locus CpG15 were positively associated with incident T2DM risk, which may suggest a possible etiologic pattern for T2DM and potentially improve T2DM prediction in rural Chinese people.

19.
Artigo em Inglês | MEDLINE | ID: mdl-32929675

RESUMO

Arsenic (As) contamination of aquatic and soil environments is a global concern, highlighting the importance of As removal via high-efficiency and low-cost removal technologies. In the present study, novel trimetallic biochar was developed through pyrolyzing corn straw impregnated with inexpensive metal Fe/Al/Zn (hydr)oxides. The results of SEM, FTIR, and XRD verified the formation of metal oxyhydroxides on the surface of the modified biochars, and the modification increased the specific surface area (SSA), total pore volume (TPV), and surface charge of the Fe/Al/Zn (hydr)oxides modified biochar (FAZ-CB). Compared with the original biochar, higher sorption rates and capacities was observed for the FAZ-CB. The maximum As (V) adsorption capacities of FAZ-CB reached 82.9 mg g-1. A pot experiment showed that application of FAZ-CB decreased bioavailable As fractions in the red soil significantly reduced the uptake of As by arugula in edible part and root (42.6 and 56.8%, respectively). The present study demonstrated the superiority of FAZ-CB in the As(V) immobilization in red soil, suggesting that it is a promising candidate for practical application for As immobilization. Therefore, FAZ-CB can be used as a promising functionalized biochar to remediate As contaminated red soil.

20.
J Diabetes Complications ; : 107712, 2020 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-32919864

RESUMO

BACKGROUND: We aimed to evaluate the association of the ratio of non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol (non-HDL-C/HDL-C) and its dynamic changes with incident type 2 diabetes mellitus (T2DM). METHODS: A total of 11,487 nondiabetic participants ≥18 years old in rural China were recruited in 2007-2008 and followed up in 2013-2014. A Cox proportional-hazards model was used to assess the risk of incident T2DM by quartiles of baseline non-HDL-C/HDL-C ratio and dynamic absolute and relative changes in non-HDL-C/HDL-C ratio, estimating hazard ratios (HRs) and 95% confidence intervals (CIs). RESULTS: Risk of incident T2DM was increased with quartiles 2, 3, and 4 versus quartile 1 of baseline non-HDL-C/HDL-C ratio (HR 1.46 [95% CI 1.08-1.98], 1.51 [1.12-2.03], and 2.16 [1.62-2.88], Ptrend < 0.001). As compared with stable non-HDL-C/HDL-C ratio during follow-up, an absolute gain in non-HDL-C/HDL-C ratio was associated with increased risk of T2DM (HR 1.67 [95% CI 1.25-2.24] for quartile 3 and 2.00 [1.52-2.61] for quartile 4). A relative increase in non-HDL-C/HDL-C ratio was also associated with increased risk of T2DM (HR 1.56 [95% CI 1.19-2.04] for quartile 3 and 1.97 [1.49-2.60] for quartile 4). Subgroup analyses showed that the association of non-HDL-C/HDL-C ratio with T2DM risk remained consistent. CONCLUSIONS: Increased non-HDL-C/HDL-C ratio is associated with increased risk of incident T2DM among rural Chinese adults, so the index may be an important indicator for identifying individuals at T2DM risk.

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