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1.
Poult Sci ; 103(6): 103724, 2024 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-38701630

RESUMO

Sertoli cells (SC) are a type of important cells in the testes, which can provide transport proteins, regulatory proteins, growth factors, and other cytokines for the spermatogenic process. They participate in the regulation of the maturation and differentiation of spermatogenic cells and play an important supporting role in the migration, proliferation, and differentiation of germ cells at all levels in the testes. Previous studies found differential expression of LINC9137, miR-140-3p, and Sodium/Potassium Transporting ATPase Interacting 3 (NKAIN3) genesin high and low sperm motility goose testicular tissues. This study investigated the effects of the LINC9137-miR-140-3p-NKAIN3 signal axis on the proliferation and apoptosis of goose testicular sertoli cells at the cellular level, respectively. The results showed that through acridine orange staining, oil red O staining, Alkaline phosphatase (AKP) staining, and RT qPCR assay, it was comprehensively identified that the cultured testicular sertoli cells were purified in vitro. Through the dual luciferase activity detection test, it was found that LINC9137 has a targeted binding site with miR-140-3p and NKAIN3. In addition, this study found that overexpression of miR-140-3p significantly inhibited the expression of LINC9137 and NKAIN3 in sertoli cells, and their expression was significantly increased when miR-140-3p was interfered with. By measuring cell proliferation activity and apoptosis related gene expression, it was found that overexpression of LINC9137 decreased cell proliferation activity (P > 0.05), while the expression level of apoptosis factor Bcl2 Associated X Protein (Bax)/B-cell lymphoma-2 (Bcl2) increased (P > 0.05). On the contrary, when interfering with LINC9137, the cell proliferation activity of sertoli cells was significantly increased (P < 0.01), and the expression level of apoptosis factor Bax/Bcl2 was significantly reduced (P < 0.05); The effect of miR-140-3p on the proliferation and apoptosis of sertoli cells is opposite to that of LINC9137. Meanwhile, this study co transfected overexpressed LINC9137 and miR-140-3p plasmids into sertoli cells, and found that the effect of LINC9137 overexpression on supporting cell proliferation was weakened by miR-140-3p. This study elucidates the role and function of the LINC9137 miR-140-3p-NKAIN3 signaling axis in the development of goose testes and spermatogenesis, establishes a regulatory network related to spermatogenesis, and provides a theoretical basis for studying the genetic regulation of goose spermatogenesis.

2.
Comput Methods Programs Biomed ; 240: 107676, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37343376

RESUMO

BACKGROUND AND OBJECTIVE: Electrocardiographic imaging (ECGI) has emerged as a non-invasive approach to identify atrial fibrillation (AF) driver sources. This paper aims to collect and review the current research literature on the ECGI inverse problem, summarize the research progress, and propose potential research directions for the future. METHODS AND RESULTS: The effectiveness and feasibility of using ECGI to map AF driver sources may be influenced by several factors, such as inaccuracies in the atrial model due to heart movement or deformation, noise interference in high-density body surface potential (BSP), inconvenient and time-consuming BSP acquisition, errors in solving the inverse problem, and incomplete interpretation of the AF driving source information derived from the reconstructed epicardial potential. We review the current research progress on these factors and discuss possible improvement directions. Additionally, we highlight the limitations of ECGI itself, including the lack of a gold standard to validate the accuracy of ECGI technology in locating AF drivers and the challenges associated with guiding AF ablation based on post-processed epicardial potentials due to the intrinsic difference between epicardial and endocardial potentials. CONCLUSIONS: Before performing ablation, ECGI can provide operators with predictive information about the underlying locations of AF driver by non-invasively and globally mapping the biatrial electrical activity. In the future, endocardial catheter mapping technology may benefit from the use of ECGI to enhance the diagnosis and ablation of AF.


Assuntos
Fibrilação Atrial , Ablação por Cateter , Humanos , Fibrilação Atrial/diagnóstico por imagem , Mapeamento Potencial de Superfície Corporal/métodos , Diagnóstico por Imagem , Eletrocardiografia/métodos , Átrios do Coração/diagnóstico por imagem , Átrios do Coração/cirurgia , Ablação por Cateter/métodos
3.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-907977

RESUMO

Objective:To investigate the prognosis value of the Child-Turcotte-Pugh (CTP), pediatrics end-stage liver disease/model for end-stage liver disease(PELD/MELD) and sequential organ failure assessment (SOFA) scores in pediatric acute liver failure (PALF) at 28 th day. Methods:Fifty-four PALF patients admitted in the Pediatric Intensive Care Unit (PICU) and Infection Department of Pediatrics, Qingdao Women′s and Children′s Hospital from June 1, 2012 to June 1, 2019 were included in the study.According to the survival of PALF patients on the 28 th day, they were divided into the survival group (28 cases) and the death group (26 cases). Baseline characte-ristics and laboratory examination data of PALF patients in both groups were collected and compared.Receiver operating characteristic (ROC) curve was used to evaluate the prognostic value of CTP, PELD/MELD and SOFA scores in PALF. Results:The mortality rate of 54 PALF patients was 48.1%.Compared with the survival group, PALF patients in the death group were significantly younger than those in survival group [11.0(3.8-39.0) months vs.14.5(7.3-84.0) months]( Z=-2.145, P=0.020). In addition, CTP, PELD/MELD and SOFA scores were significantly higher in the death group than those in survival group [14.0(11.7-15.0) vs.9.0(7.0-10.0), 32.0(29.0-36.0) vs.25.0(22.0-26.0), 13.0(11.0-16.0) vs.6.0(4.0-7.0)]( Z=-5.095, -4.894, -5.502, all P<0.05). Serum lactate level, blood ammonia level, total bilirubin, direct bilirubin and international normalized ratio were significantly higher in the death group than those in survival group [3.4(2.1-5.3) mmol/L vs.1.5(0.8-2.3) mmol/L, 69.5(46.9-102.9) μmol/L vs.41.7(27.3-50.3) μmol/L, 173.0(97.0-237.2) μmol/L vs.71.9(62.0-136.9) μmol/L, 132.3(53.6-206.2)μmol/L vs.59.3(62.0-99.7) μmol/L, 2.6(1.8-3.5) vs.1.7(1.5-1.9)]( Z=-4.027, -3.220, -2.649, -2.648, -3.807, all P<0.05). Prothrombin time (PT) was significantly prolonged in the death group than that of survival group [27.5(19.2-41.9)s vs.17.8(16.9-22.2)s]( Z=-3.489, P<0.05). Compared with those of survival group, serum albumin, alanine transaminase (ALT) and alpha fetoprotein (AFP) levels were significantly lower in the death group [(30.9±1.0) g/L vs.(33.6±0.9) g/L, 379.2(163.3-880.3) U/L vs.962.5(457.0-1 657.3) U/L, 7.5(0.7-115.8) μg/L vs.22.1(7.9-91.3) μg/L]( t=2.049, Z=-2.510, -2.342, respectively, all P<0.05). The incidence of alimentary tract hemorrhage was significantly higher in the death group than that of survival group (22/26 cases vs.11/28 cases)( χ2=13.340, P<0.05). The cut-off value of CTP, PELD/MELD and SOFA scores in predicting the prognosis of PALF were 11.5, 28.5 and 10.0, respectively.Among the three scoring systems, the specificity and positive predictive value of SOFA scores remained the highest.The sensitivity and specific of a combination of three scoring systems in predicting the prognosis of PALF were 92.3% and 89.3%, respectively, and its Youden index was the highest than that of a single scoring of either CTP, PELD/MELD or SOFA ( Z=2.19, P<0.05). Conclusions:CTP, PELD/MELD and SOFA scores have high predictive value for the short-term prognosis of PALF.The combined detection of the three scoring systems can improve the forecasting efficiency of PALD.

4.
Frontiers of Medicine ; (4): 887-902, 2021.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-922517

RESUMO

Monocarboxylic acid transporter 1 (MCT1) maintains axonal function by transferring lactic acid from oligodendrocytes to axons. Subarachnoid hemorrhage (SAH) induces white matter injury, but the involvement of MCT1 is unclear. In this study, the SAH model of adult male Sprague-Dawley rats was used to explore the role of MCT1 in white matter injury after SAH. At 48 h after SAH, oligodendrocyte MCT1 was significantly reduced, and the exogenous overexpression of MCT1 significantly improved white matter integrity and long-term cognitive function. Motor training after SAH significantly increased the number of ITPR2


Assuntos
Animais , Masculino , Ratos , MicroRNAs/genética , Transportadores de Ácidos Monocarboxílicos/genética , Ratos Sprague-Dawley , Hemorragia Subaracnóidea , Simportadores/genética , Substância Branca/lesões
5.
Preprint em Inglês | medRxiv | ID: ppmedrxiv-20088740

RESUMO

We compared the ability of 2 commercial molecular amplification assays [RealTime SARS-CoV-2 on the m2000 (Abbott) and ID NOW COVID-19 (Abbott)] and a laboratory-developed test [modified CDC 2019-nCoV RT-PCR assay with RNA extraction by eMag(R) (bioMerieux) and amplification on QuantStudio 6 or ABI 7500 Real-Time PCR System (Life Technologies)] to detect SARS-CoV-2 RNA in upper respiratory tract specimens. Discrepant results were adjudicated by medical record review. 200 nasopharyngeal swab specimens in viral transport medium (VTM) were collected from symptomatic patients between March 27 and April 9, 2020. Results were concordant for 167 specimens (83.5% overall agreement), including 94 positive and 73 negative specimens. The RealTime SARS-CoV-2 assay on the m2000 yielded 33 additional positive results, 25 of which were also positive by the modified CDC assay but not by the ID NOW COVID-19 assay. In a follow-up evaluation, 97 patients for whom a dry nasal swab specimen yielded negative results by the ID NOW COVID-19 assay had a paired nasopharyngeal swab specimen collected in VTM and tested by the RealTime SARS-CoV-2 assay; SARS-CoV-2 RNA was detected in 13 (13.4%) of these specimens. Medical record review deemed all discrepant results to be true positives. The ID NOW COVID-19 test was the easiest to perform and provided a result in the shortest time: as soon as 5 minutes for positive and 13 minutes for negative result. The RealTime SARS-CoV-2 assay on the m2000 detected more cases of COVID-19 infection than the modified CDC assay or the ID NOW COVID-19 test.

6.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-864035

RESUMO

Objective:To discuss the incidence rate and influencing factors of postencephalitic epilepsy (PE) in children with viral encephalitis at acute symptomatic seizure(ASS).Methods:The data of 132 children with ASS in the First Affiliated Hospital of Zhengzhou University from September 2013 to July 2018 were retrospectively analyzed, and the patients were divided into PE group (62 cases) and non-PE group (70 cases) according to whether they had PE at final follow-up.The risk factors of PE in children with ASS were analyzed using the multivariate Logistic regre-ssion methods. Results:The incidence of PE in children with ASS was 46.97% (62/132 cases). There were statistically significant differences in terms of psychological and behavioral abnormalities[19.4%(12/62 cases) vs.2.9%(2/70 cases)], repetitive seizures (more than 5 seizures) [67.7%(42/62 cases) vs.17.1%(12/70 cases)], status epilepticus (SE) [30.6%(19/62 cases) vs.11.4%(8/70 cases)], generalized seizures [72.6%(45/62 cases) vs.88.6%(62/70 cases)], endotracheal intubation [21.0%(13/62 cases) vs.2.9%(2/70 cases)], the duration of fever [5.5(3.0, 11.0) d vs.3.0(2.0, 6.0) d], the duration in the intensive care unit (ICU) [13(5, 21) d vs.6(3, 8) d], electroencephalography epileptiform discharges [49.1%(27/55 cases) vs.6.8%(4/59 cases)], presence of subcortical involvement on neuroimaging [37.3%(22/55 cases) vs.20.3%(14/59 cases)] in children with ASS between the PE group and the non-PE group(all P<0.05). The multivariate Logistic regression analysis showed that repetitive seizures (more than 5 seizures) ( OR=5.256, 95% CI: 1.538-17.961, P=0.008), SE( OR=6.003, 95% CI: 1.411-25.539, P=0.015), electroencephalography epileptiform discharges ( OR=36.693, 95% CI: 7.031-191.485, P<0.01) and the duration in ICU ( OR=1.170, 95% CI: 1.058-1.298, P=0.002) were the risk factors for PE in children with ASS. Conclusions:The incidence rate of PE in children with ASS is high.Children with ASS are more likely to develop into PE if they have repetitive seizures (more than 5 seizures), SE, electroencephalography epileptiform discharges, and the longer duration in ICU.

7.
Journal of Leukemia & Lymphoma ; (12): 378-381, 2020.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-862847

RESUMO

The related changes of bone marrow microenvironment (especially mesenchymal stem cells and osteoblasts) would lead to the occurrence of leukemia. Bone morphogenetic protein (BMP) is considered to be the only known morphogenetic factor capable of inducing differentiation of mesenchymal stem cells into osteoblasts, and its role in regulating the occurrence and development of leukemia in the bone marrow microenvironment has been paid more attention. The studies found that acute myeloid leukemia derived-mesenchymal stem cells induced the morphologic transformation and resistance of K562-ADM through BMP. This paper reviews the related research of BMP in order to provide new targets and directions for the research and treatment of leukemia.

8.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-743938

RESUMO

Objective To study the changes and significance of serum intestinal fatty acid binding protein (IFABP) in children with traumatic brain injury(TBI) complicaled with acute gastrointestinal injury (AGI). Methods A total of 95 children suffering from TBI hospitalized in the PICU of the First Affiliated Hospital of Zhengzhou University from January 2017 to March 2018 were enrolled in the study. According to the modified Glasgow coma score combined with clinical classification criteria for acute closed head injury, the cases were devided into mild(43 cases),moderate (23 cases),and severe(29 cases). Children were gra-ded according to AGI (AGI Ⅰ42 cases,AGI Ⅱ 30 cases,AGI Ⅲ 13 cases,and AGI Ⅳ 10 cases). Thirty healthy children who underwent physical examination at outpatient service were enrolled as the control group. Blood samples were collected at the time of admission and on the 3rd day after admission. Serum IFABP was detected by ELISA,and the differences of serum IFABP concentrations were compared among groups. The correlations between IFABP with TBI classification and AGI grade were analyzed. The receiver operating characteristic (ROC) curve was drawn,and the predictive values of IFABP for the diagnosis of children with TBI complicated with AGI were evaluated. Results On the day of admission,the serum levels of IFABP in mild,moderate and severe brain injury group were significantly higher than that in control group (all P <0. 01). And serum IFABP concentration gradually increased with the increase of brain injury (all P < 0. 01).Serum IFABP levels in children with AGI grade Ⅰto Ⅳ were significantly higher than those in control group (all P < 0. 01). The levels of serum IFABP also increased with the increase of AGI level (all P < 0. 01). The concentration of serum IFABP was positively correlated with the grade of TBI and AGI (rs = 0. 82,P < 0. 01;rs = 0. 70,P < 0. 01). In each group,the levels of serum IFABP on the 3rd day after admission were lower than those on admission (all P < 0. 01). The ROC curve analysis showed that serum IFABP was of high diag-nostic value in children with TBI complicated with AGI,and the area under the ROC curve was 0. 88. When the cutoff value of IFABP was 431. 36 ng/ L,the sensitivity and specificity were 71. 61% and 90. 00% ,re-spectively. Conclusion Serum IFABP can be used as a sensitive indicator for the early diagnosis and disease assessment in children with TBI complicated with gastrointestinal dysfunction.

9.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-756119

RESUMO

We reported a case of monochorionic monoamniotic twins discordant for anencephaly diagnosed by second-trimester ultrasonography at the First Affiliated Hospital of Fujian Medical University.Ultrasound at seven weeks of gestation showed only one gestational sac with an embryo inside.Another 12 gestational weeks' ultrasound scan performed at another hospital found one gestational sac and one fetus (crown-rump length was 6.11 cm and nuchal translucency was 0.11 cm) in the upper-middle uterine cavity.The ultrasound examination at 22+6 gestational weeks identified one placenta and two fetuses without obvious diaphragm echo in between.Although no structural abnormality was observed in one fetus,frog-like eyes,absence of skull image and brain tissue echo were presented in the other fetus.The patient was transferred to a higher level hospital and was successfully performed radiofrequency ablation for selective reduction at 23+4 weeks of gestation.At 35 weeks,a premature live boy and an anencephalic stillbirth fetus were born vaginally after premature rupture of membranes.The baby boy was healthy at follow-up at four months old.

10.
BMC Cancer ; 18(1): 411, 2018 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-29649989

RESUMO

BACKGROUND: Brucea javanica oil emulsion (BJOE) is traditional Chinese medicine with implicated anti-tumor activity, which has been used for treating lung cancer in China. The aim of this investigation was to evaluate the effects and safety of intrapleural injection of BJOE in treating malignant pleural effusion (MPE). METHODS: The randomised controlled trials (RCTs) on the effects and safety of BJOE in treating MPE were searched from electronic medical database including MEDLINE, SCI, EMBASE, Cochrance Library and CNKI. A total of 14 RCTs with 1085 patients were involved in this meta-analysis. RESULTS: The overall response rate (ORR) of traditional chemotherapy drugs plus BJOE was higher than that of traditional chemotherapy drugs alone (p = 0.001; odds ratio = 1.39). Meanwhile, the combination of BJOE and traditional chemotherapy drugs improved the quality of life (QOL) of patients with MPE (p < 0.001; odds ratio = 1.56) compared with traditional chemotherapy drugs alone. Moreover, the participation of BJOE reduced the myelotoxicity and digestive reactions caused by traditional chemotherapy drugs (p < 0.05). CONCLUSIONS: The efficacy and safety of traditional chemotherapy drugs plus BJOE was superior to traditional chemotherapy drugs alone via intrapleural injection in controlling MPE, which suggested that BJOE can be used to treat MPE.


Assuntos
Antineoplásicos Fitogênicos/administração & dosagem , Antineoplásicos Fitogênicos/química , Brucea/química , Óleos de Plantas/administração & dosagem , Óleos de Plantas/química , Derrame Pleural Maligno/tratamento farmacológico , Emulsões , Humanos , Razão de Chances , Perfusão , Derrame Pleural Maligno/patologia , Viés de Publicação , Qualidade de Vida , Resultado do Tratamento
11.
China Pharmacy ; (12): 1668-1672, 2018.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-704866

RESUMO

OBJECTIVE:To excavate the drug use rules of type 2 diabetes,and to provide reference for clinical treatment. METHODS:Clinical data of 807 patients with type 2 diabetes were collected from our hospital during Jan. 2012-Jun. 2017. Complex network and subnetworks for drug compatibility and their visual chromatograms were established by Gephi 0.9.1 software. MATLAB R2014a software was used for topic model mining. The complex network,subnetworks and the overlapping structures of community were analyzed in respects of node distance and degree centrality. Core drugs for type 2 diabetes were screened. RESULTS & CONCLUSIONS:The drug complication complex network established in the study included 119 nodes and 24 412 edges with maximum edge weight of 378. Drugs with high frequency included Insulin aspart injection,Fluvastatin sodium extended release tablets,Metformin hydrochloride tablets,Acarbose tablets,etc. Average distance of network was 1.89,and maximum distance was 4. The distance of 89.90% drugs was 1 or 2,and the links between the various drugs were close. Insulin aspart injection(0.914),Metformin hydrochloride tablets(0.887)and Voglibose tablets(0.866)were core drugs of the network. Totally 4 typical topics were excavated, including peripheral neuropathy, peripheral vascular disease, abnormal lipid metabolism and microangiopathy. Results of subnetwork analysis showed that typical topics were based on hypoglycemic therapy and supplemented by neurotrophic drugs,antihypertensive drugs,lipid regulating drugs and drugs for improvement of retinopathy. Complex network analysis showed that drug use regularity was in line with related guideline of type 2 diabetes and clinical practice. The method has practical significance of data mining in clinic.

12.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-700525

RESUMO

Objective To explore the application effect of formative assessment on the standardized training in pediatric residents.Methods A total of 36 pediatric residents who attended the standardized training in affiliated hospital of Nantong university were randomly divided into 2 groups (18 in the experimental group and 18 in the control group) and trained in accordance with the standardized training content and standard of the national resident of pediatrics.Residents in the experimental group received formative assessment,with total score including process assessment (35%),graduation examination (50%),medicalethics (5%),reading report (5%) and work attendance (5%).While the other 18 residents in the control group received traditional assessment,with final score of graduation examination.The effectiveness of formative assessment was conducted via analysis of test score and self evaluation.The SPSS 17.0 was used for statistics,independent sample t test was used to perform inter-group comparison and Pearson correlation coefficient was used to demonstrate the correlation.P<0.05 signifies statistically significant differences.Results In the experimental group,the process evaluation and the graduation examination were both positively related with the total score (r=0.953,r=0.966,P=0.00).In the graduation examination,the scores of clinical practice skills test and case analysis in the experimental group were both higher than those in the control group [(12.03 ± 1.51) vs.(10.83 ± 1.42),(12.03 ± 1.40) vs.(10.83 ± 1.29),P<0.05],with statistically significant differences.The self evaluation of trainees showed that the clinical-inference and decisionmaking ability,the self-study ability,scores of attitudes and professionalism and total scores in the experimental group were higher than those in the control group,with statistically significant differences (P<0.05).Conclusion The application of formative evaluation on the standardized training in pediatric residents is beneficial to improve the training effect and is worth popularizing.

13.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-752138

RESUMO

Traditional Chinese Medicine (TCM) healthcare culture presents a long history and has broad connotations.TCM healthcare culture in the new era receives increasing concerns due to its full-blown theoretical system as well as various healthcare models, which is integrated with modern science and technology and business model. This article focuses on the new opportunities and challenges to the development of Chinese medicine healthcare culture industry in the new era and based on its major features, comprehensively analyzes the innovation and development issues of TCM healthcare culture from multi-dimensions, including integration of international strategy, social needs and technology development, etc.

14.
China Pharmacy ; (12): 3208-3212, 2017.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-612240

RESUMO

OBJECTIVE:To explore the application of topic model in the study of type 2 diabetes treatment plan. METHODS:Clinical data of 650 inpatients with type 2 diabetes in our hospital during Jan. 2012-Jun. 2016 were analyzed retrospectively. The data of clinical diagnosis,lab indexes and clinical drug use were exchanged,summarized and merged by MATLAB R2014a software. Latent Dirichlet allocation and author topic model were adopted to extract the typical topics with topic probability value>0.1,and the topics was described by the complications with cumulative probability value>0.5. RESULTS:A total of 62 complications words,16 abnormal laboratory indexes groups and 20 treatment plans were obtained. A total of 4 typical topics were excavated(cumulative probability values for the first few complications were 0.8786,0.8247,0.8215,0.7536;topic probability value were 0.3364,0.2773,0.2035,0.1176,respectively) and were mainly characterized by peripheral neuropathy,peripheral vascular disease,abnormal lipid metabolism and microvascular lesions;abnormal lab indexes groups met the above characteristics. The complications with high distribution rate included diabetic peripheral neuropathy (0.5787), hypertension (0.3631),atherosclerosis (0.2789),hyperlipidemia (0.4578) and diabetic retinopathy (0.3143);main drugs included Insulin aspart injection,Insulin injection,Methylcobalamin dispersible tablets,etc. CONCLUSIONS:The complications of type 2 diabetes are characterized by peripheral neuropathy,peripheral vascular disease,abnormal lipid metabolism and microvascular lesions. The medication rules with clinical significance can be extracted from the clinical data by topic model.

15.
Chinese Journal of Immunology ; (12): 437-440, 2017.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-510455

RESUMO

Objective:To study on the changes of the ratio of serum NEFA/ALB in children with primary nephrotic syndrome , and to provide new indicators for the diagnosis and severity assessment of clinical PNS .Methods:The levels of serum albumin ( ALB) , free fatty acid (NEFA),urea nitrogen(BUN),uric acid(UA),creatinine(Scr),total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol ( LDL-C) ,high density lipoprotein cholesterol ( HDL-C) were quantitatively measured in 52 patients with PNS in acute stage ( acute stage group ) and 34 patients with PNS in complete remission ( remission stage group ) and 50 healthy children ( con-trol group).The lipid/lipoprotein and renal function parameters were also analyzed .Results: Compared with controls group ,the levels of serum ALB,HDL-C in acute group and remission group decreased significantly ( P<0.05 ) ,but the levels of serum NEFA/ALB,TC, TG,LDL-C,UA,BUN,Scr in acute group increased significantly ( P<0.05 ) ,the levels of serum TG ,LDL-C in acute group increased significantly (P<0.05).Compared with remission group,the levels of serum ALB in acute group decreased significantly (P<0.05), but the levels of serum NEFA/ALB,TC,TG,LDL-C,UA,BUN,Scr increased significantly (P<0.05).In PNS children,the ratio of NEFA to ALB was positively correlated with the levels of TC ,TG,LDL-C,UA,BUN (P<0.05),while negatively correlated with ALB (P<0.05).Multiple linear regression analysis showed that the ratio of NEFA to ALB had an independent association with BUN levels (β=0.045,t=1.602,P=0.003).Conclusion:In the development process of PNS disease ,serum ratio of NEFA to ALB increased in-dicates impaired kidney function or reduced kidney function ,and it can help doctor assess and judge the clinical condition .

16.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-621076

RESUMO

Objective To investigate the relationship between serum three triacylglycerol lipase (ATGL) and inflammatory factors in patients with coronary atherosclerotic heart disease (CHD).Methods 135 cases of CHD patients,and 50 healthy people were enrolled in the study.CHD patients were divided into stable angina pectoris (SAP) group (53 cases),unstable angina pectoris (UAP) group(46 cases) and acute myocardial infarction (AMI) group (36 cases) according to relevant diagnostic criteria.The serum levels of ATGL,tumor necrosis factor (TNF-α),interleukin-6(IL-6), interleukin-10(IL-10),1,25 dihydroxy vitamin D3[1,25(OH)2D3] were measured by ELISA, and statistical treatment was performed.Results The levels of ATGL,1,25(OH)2D3 in CHD group were lower than that in control group,and the levels of TNF-α,IL-6 and IL-10 were higher than those of control group(P<0.05).Comparison among different clinical types of CHD patients showed that ATGL,1,25 (OH)2D3 levels of AMI group were significantly lower than those of SAP group and UAP group,while TNF-α,IL-6 and IL-10 were higher than those of SAP group and UAP group(P<0.05);the levels of ATGL,1,25(OH)2D3 in UAP group were lower than those in SAP group,and TNF-α and IL-10 were higher than those in SAP group(P<0.05).Spearman correlation analysis showed that in the CHD group the serum levels of ATGL negatively correlated with TG,LDL-C,LP (a),TNF-α,IL-6,IL-10(r=-0.289,-0.227,-0.359,-0.243,-0.205,-0.337,P<0.05),while positively correlated with age,HDL-C,1,25(OH)2D3(r=0.267,0.356,0.229,P<0.05).Conclusion The ATGL levels in patients with CHD decreases with the increase of inflammatory factors,and plays a certain anti-inflammatory role in the progression of CHD.It can be used as a monitoring indicator to reflex the progress of CHD.

17.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-619219

RESUMO

Objective:To assess the effects of dental treatment under general anesthesia on the life quality of children and their families.Methods:59 children aged 3 to 6-year-old were included.Parents were required to complete the early childhood oral health impact scales(ECOHIS) before and 1 month after dental treatment under general anesthesia.The questionnaire includes child impact section(CIS) and family impact section(FIS).Data were analyzed using SPSS version 19.0.Results:55 out of the 59 parents completed the qustionnaire.After treatment the total ECOHIS scores decreased by 51% (P < 0.001),overall child impact section scores decreased by 50% (P <0.001),and family impact section scores decreased by 53% (P <0.001),revealing a large effect size(ES) for the child(1.4) and family (4.0) sections of the ECOHIS.Conclusion:Dental treatment under general anesthesia for uncooperative young children with extensive dental problems can improve the quality of life of both children and their families.

18.
Vet Microbiol ; 197: 62-67, 2016 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-27938684

RESUMO

Forty-three fowl adenovirus (FAdV) strains were isolated in China from 2007 to 2014 from poultry and ostriches with inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS). Phylogenetic analysis showed that 28/43 strains clustered into Fowl aviadenovirus D (FAdV-D) and 9/43 strains clustered into FAdV-E. FAdV-C included three isolates of ostrich origin, one of goose origin and two of chicken origin. Based on hexon loop 1 gene sequencing analysis, these viruses were genetically related to FAdV-4, FAdV-8a, FAdV-8b and FAdV-11, of which FAdV-11 was dominant. The isolation in 2014 of three FAdV strains belonging to serotype 4 from ostrich flocks is to our knowledge the first finding of FAdV-4 infection and HPS cases in ostriches. Epidemiological analysis showed that FAdV has been circulating in northern and eastern China, where more than 50% of layers and broilers are raised. The hosts of this pathogen included broilers, layers, geese and ostriches. IBH and HPS cases had a sporadic or cluster distribution from 2007 to 2013; however, since 2014 the number of cases has increased sharply. To control FAdV, strict biosecurity protection measures are necessary and a multivalent vaccine may be needed.


Assuntos
Anseriformes , Aviadenovirus/genética , Doenças das Aves/virologia , Galinhas , Struthioniformes , Animais , Doenças das Aves/epidemiologia , China/epidemiologia , Filogenia
19.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-814953

RESUMO

OBJECTIVE@#To explore the effect of general anesthesia combined with epidural anesthesia and patient-controlled epidural analgesia on the incidence of the post-operative cognitive dysfunction (POCD) in elderly patients.
@*METHODS@#A total of 100 elderly patients with gastric cancer, who underwent radical gastrectomy in Yan'an People's Hospital from March 2012 to March 2015, were randomly divided into 3 groups: a general anesthesia and patient-controlled intravenous analgesia (PCIA) (Group I, n=35), a general anesthesia combined with epidural anesthesia and PCIA (Group II, n=30), and a general anesthesia combined with epidural anesthesia and patient-controlled epidural analgesia (PCEA) (Group III, n=35). There was no significant difference in age, gender, body mass index (BMI), length of operation, American Society of Anesthesiologists (ASA) grade and Mini-Mental State Examination (MMSE) score (all P>0.05). The cognitive function was assessed at the 1st day before operation and at the 6 h, 12 h, 2 d and 6 d after operation by the Mini-Mental State Examination test (MMSE), and the incidence of POCD was compared among the 3 groups. The mean arterial pressure (MAP), blood glucose, cortisol and tumor necrosis factor α (TNF-α) content during the operation were considered. The dosage of sevoflurane (SEVO) in the operation process and the pain score at the 6, 12 and 24 h after operation were recorded.
@*RESULTS@#The incidence of POCD at 6 h was 45.71% in the Group I and 34.28% in the Group II, which were higher than that in the Group III (10.00%). The incidence of POCD at 12 h was 34.29% in the Group I and 28.57% in the Group II, which were higher than that in the Group III (3.33%). The incidence of POCD at the 2 d was 28.57% in the Group I and 17.14% in the Group II, which were higher than that in the Group III (0). The incidence of POCD at 6 d was 17.14% in the Group I and 2.85% in the Group II, which were higher than that in the Group III (0). The blood glucose, cortisol and TNF-α content were consistent with the incidence of POCD, with significant difference (all P<0.05). The amplitude of MAP in the Group II and the Group III was significantly less than that in the Group I, and the postoperative analgesia effect in the Group III was obviously better than that in the other two groups (both P<0.05).
@*CONCLUSION@#The general anesthesia combined with epidural anesthesia and patient-controlled epidural analgesia can effectively maintain the stable hemodynamic status in the patients and display inhibitory effect on postoperative cognitive dysfunction.


Assuntos
Humanos , Analgesia Epidural , Analgesia Controlada pelo Paciente , Anestesia Epidural , Anestesia Geral , Cognição , Transtornos Cognitivos , Disfunção Cognitiva , Gastrectomia , Incidência , Éteres Metílicos , Testes Neuropsicológicos , Complicações Pós-Operatórias , Sevoflurano , Neoplasias Gástricas , Fator de Necrose Tumoral alfa
20.
The Journal of Practical Medicine ; (24): 396-398,399, 2016.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-603176

RESUMO

Objective To investigate the expressions of TK1 (thymidine kinase 1) in PHC (primary hepatic carcinoma). Methods TK1 and AFP in serum of 33 cases of PHC (primary hepatic carcinoma), 38 cases of hepatic cirrhosis,36 cases of hepatitis and 35 cases of healthy people were detected by means of Western blot—enhanced chemiluminecence and electrochemiluminescence. Results The difference of TK1 level in PHC group indicated significance when compared with that in hepatic cirrhosis group , hepatitis group and control group (U value was 436.4, 352.1, 163.6, respectively, all P 0.05). Kaplan-Meier curve analysis revealed that PHC patients with TK1≤ 2.0 pmol/L had a significantly shortened overall survival when compared with those with TK1 > 2.0 pmol/L (χ2 = 3.954,P < 0.05). Multivariable Cox regression analysis indicated that the level of TK1 and TNM stage were the independent risk factors for patients with PHC (all P <0.05). Conclusions The detection of TK1 has a certain clinical value in the diagnosis, monitoring and evaluation of the prognosis of the PHC.

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