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1.
J Pharm Biomed Anal ; 177: 112813, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31472326

RESUMO

Differentiated composition in precursor ions for different subclasses of ginsenosides in the negative electrospray-ionization mode has been reported, which lays a foundation for the sorted and untargeted identification of ginsenosides. Carboxyl-free ginsenosides simultaneously from Panax ginseng, P. quinquefolius, and P. notoginseng, were comprehensively characterized and statistically compared. A neutral loss/product ion scan (NL-PIS) incorporated untargeted profiling approach, coupled to ultra-high performance liquid chromatography, was developed on a linear ion-trap/Orbitrap mass spectrometer for characterizing carboxyl-free ginsenosides. It incorporated in-source fragmentation (ISF) full scan-MS1, mass tag-MS2, and product ion scan-MS3. Sixty batches of ginseng samples were analyzed by metabolomics workflows for the discovery of ginsenoside markers. Using formic acid (FA) as the additive, carboxyl-free ginsenosides (protopanaxadiol-type, protopanaxatriol-type, and octillol-type) gave predominant FA-adducts, while rich deprotonated molecules were observed for carboxyl-containing ginsenosides (oleanolic acid-type and malonylated) when source-induced dissociation (SID) was set at 0 V. Based on the NL transition [M+FA‒H]- > [M-H]- and the characteristic sapogenin product ions, a NL-PIS approach was established. It took advantage of the efficient full-information acquisition of ISF-MS1 (SID: 50 V), the high specificity of mass tag (NL: 46.0055 Da)-induced MS2 fragmentation, and the substructure fragmentation of product ion scan-MS3. We could characterize 216 carboxyl-free ginsenosides, and 21 thereof were potentially diagnostic for the species differentiation. Conclusively, sorted and untargeted characterization of the carboxyl-free ginsenosides was achieved by the established NL-PIS approach. In contrast to the conventional NL or PIS-based survey scan strategies, the high-accuracy MSn data obtained can enable more reliable identification of ginsenosides.

2.
J Med Virol ; 92(1): 34-44, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31429942

RESUMO

Mounting evidence indicates that serum soluble E-cadherin (sE-cadherin) serves as an important player in various physiological and pathological processes. However, the crosstalk between serum sE-cadherin and oxidative stress in chronic hepatitis B (CHB) remains to be illustrated. The main purpose of this study is to explore the molecular mechanisms underlying the function of sE-cadherin in CHB virus infection. Levels of serum sE-cadherin, total antioxidant capacity (TAC), glutathione (GSH), superoxide dismutase (SOD), total oxidant activity (TOA), NADPH oxidase 2 (NOX2), and malondialdehyde (MDA), from 51 patients with hepatitis B envelope antigen (HBeAg)-negative CHB, 54 patients with HBeAg-positive CHB, and 109 healthy individuals were detected by enzyme-linked immunosorbent assay. In our study, patients with CHB showed significantly higher serum sE-cadherin levels than healthy individuals (P < .01). Furthermore, we also found that the serum sE-cadherin levels were significantly negatively correlated with TAC, antioxidant enzymes (GSH and SOD) in patients with CHB, and that serum sE-cadherin concentrations were significantly positively correlated with liver enzyme markers (alanine transaminase and aspartate aminotransferase) and oxidative markers (TOA, NOX2, and MDA) in patients with CHB. Therefore, serum sE-cadherin may act as a new candidate biomarker for reflecting inflammation and oxidative stress status in the development and progression of hepatitis B virus infection.

3.
J Enzyme Inhib Med Chem ; 35(1): 139-144, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31724435

RESUMO

A series of naphthalene-chalcone derivatives (3a-3t) were prepared and evaluated as tubulin polymerisation inhibitor for the treatment of breast cancer. All compounds were evaluated for their antiproliferative activity against MCF-7 cell line. The most of compounds displayed potent antiproliferative activity. Among them, compound 3a displayed the most potent antiproliferative activity with an IC50 value of 1.42 ± 0.15 µM, as compared to cisplatin (IC50 = 15.24 ± 1.27 µM). Additionally, the promising compound 3a demonstrated relatively lower cytotoxicity on normal cell line (HEK293) compared to tumour cell line. Furthermore, compound 3a was found to induce significant cell cycle arrest at the G2/M phase and cell apoptosis. Compound 3a displayed potent tubulin polymerisation inhibitory activity with an IC50 value of 8.4 µM, which was slightly more active than the reference compound colchicine (IC50 = 10.6 µM). Molecular docking analysis suggested that 3a interact and bind at the colchicine binding site of the tubulin.

4.
Lung Cancer ; 139: 18-21, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31704279

RESUMO

OBJECTIVES: While immune checkpoint inhibitor (ICI) therapy has excellent efficacy in treating multiple cancers, some patients experience accelerated disease progression, defined as hyper-progressive disease (HPD). However, the characteristics of HPD, especially in patients with non-small-cell lung cancer (NSCLC), remain to be elucidated. MATERIALS AND METHODS: We report about an advanced NSCLC patient with striking disease progression, defined as HPD by existing standards, after the administration of pembrolizumab. We also reviewed related studies to discuss the definition, relative factors, and prognosis of HPD. RESULTS AND CONCLUSION: This case of NSCLC with HPD had a series of characteristics not widely described in HPD cases previously reported, suggesting the potential complexity of this phenomenon and the necessity to study its characteristics and to more closely monitor patients who are administered ICIs.

5.
Biologicals ; 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31753578

RESUMO

The traditional vaccine strains, such as LaSota, do not completely prevent the shedding of NDV. An ideal vaccine which could not only prevent the clinical signs, but significantly reduce the shedding of NDV is urgently needed for the eradication of ND. In this study, an NDV isolate APMV-1/Chicken/China (SC)/PT3/2016 (hereafter referred as PT3) was identified as a class Ⅰ NDV and a lentogenic strain. The antigenic relationship between PT3 and 3 other NDV strains, including vaccine strain LaSota and 2 prevalent genotype Ⅶd and Ⅵb strains were analyzed. The protective efficacy of PT3 and LaSota against challenge with genotype Ⅶd and Ⅵb strains were assessed. The antigenic analysis result showed that 4 strains belong to the single serotype and the PT3 antiserum exhibited the highest HI titer against 3 other NDV strains. The results of protective efficacy showed that both of LaSota and PT3 could provide 100% survivability for infected chickens. However, PT3 performed better in inducing higher humoral responses and reducing virus shedding than the LaSota strain. Lentogenic strains from Class I NDV appear to be promising vaccine candidates for the control of ND, and allows for the easy discrimination of field NDV and vaccine strains.

6.
Zootaxa ; 4657(1): zootaxa.4657.1.8, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31716802

RESUMO

A new species of the genus Thalassironus de Man, 1889, which was discovered from marine sediments in the South China Sea, is described here as Thalassironus filiformis sp. nov. The new species is characterized by having short outer labial setae and cephalic setae of equal length, long filiform tail, buccal cavity with three anterior teeth and three additional teeth just posterior to amphideal fovea, male with a segmented midventral precloacal seta, spicules double curved, slender with ventral ala. It is very similar to Thalassironus lynnae Keppner, 1987, but can be differentiated from the latter species by much shorter cephalic setae (8 µm vs 14-16 µm), longer tail (772-780 µm vs 300-506 µm), longer spicules (67-68 µm vs 35-40 µm), smaller value of de Man ratio "a" (66 vs 99.7-102) and different structure of spicules. An updated dichotomous key to all known species of Thalassironus is provided.


Assuntos
Enoplídios , Nematoides , Animais , China , Masculino
7.
Zootaxa ; 4603(3): zootaxa.4603.3.9, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-31717219

RESUMO

A new free-living marine nematode species of the genus Elzalia belonging to the family Xyalidae is described from the seabed sediment of the northeast region of the South China Sea. Elzalia heterospiculata sp. nov. is characterized by elongated spicules unequal in length, left spicule prominently longer than right spicule, ratio of body length to spicule length is 4.7-6.2; complex gubernaculum consisting of four sections, apophyses clavate, 12-14 µm long; and amphideal fovea positioned near the top of the head. It is similar to E. federici Castillo-Fernandez and Lambshead, 1990 and E. floresi Gerlach, 1957. However, the new species differs from the similar two species in having markedly unequal spicules, complex gubernaculum consisting of four sections, amphideal fovea close to the top of the head. Updated key to all species of Elzalia is provided.


Assuntos
Nematoides , Animais , Osso e Ossos , China , Cromadoria , Cabeça
8.
Int J Mol Sci ; 20(22)2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31739611

RESUMO

The Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus prevalent in east and southeast Asia, the Western Pacific, and northern Australia. Since viruses are obligatory intracellular pathogens, the dynamic processes of viral entry, replication, and assembly are dependent on numerous host-pathogen interactions. Efforts to identify JEV-interacting host factors are ongoing because their identification and characterization remain incomplete. Three enzymatic activities of flavivirus non-structural protein 3 (NS3), including serine protease, RNA helicase, and triphosphatase, play major roles in the flaviviruses lifecycle. To identify cellular factors that interact with NS3, we screened a human brain cDNA library using a yeast two-hybrid assay, and identified eight proteins that putatively interact with NS3: COPS5, FBLN5, PPP2CB, CRBN, DNAJB6, UBE2N, ZNF350, and GPR137B. We demonstrated that the DnaJ heat shock protein family (Hsp40) member B6 (DNAJB6) colocalizes and interacts with NS3, and has a negative regulatory function in JEV replication. We also show that loss of DNAJB6 function results in significantly increased viral replication, but does not affect viral binding or internalization. Moreover, the time-course of DNAJB6 disruption during JEV infection varies in a viral load-dependent manner, suggesting that JEV targets this host chaperone protein for viral benefit. Deciphering the modes of NS3-interacting host proteins functions in virion production will shed light on JEV pathogenic mechanisms and may also reveal new avenues for antiviral therapeutics.

9.
BMC Genomics ; 20(1): 806, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31684870

RESUMO

BACKGROUND: Transmissible gastroenteritis virus (TGEV) infection can cause acute inflammation. Long noncoding RNAs (lncRNAs) play important roles in a number of biological process including inflammation response. However, whether lncRNAs participate in TGEV-induced inflammation in porcine intestinal epithelial cells (IPECs) is largely unknown. RESULTS: In this study, the next-generation sequencing (NGS) technology was used to analyze the profiles of lncRNAs in Mock and TGEV-infected porcine intestinal epithelial cell-jejunum 2 (IPEC-J2) cell line. A total of 106 lncRNAs were differentially expressed. Many differentially expressed lncRNAs act as elements to competitively attach microRNAs (miRNAs) which target to messenger RNA (mRNAs) to mediate expression of genes that related to toll-like receptors (TLRs), NOD-like receptors (NLRs), tumor necrosis factor (TNF), and RIG-I-like receptors (RLRs) pathways. Functional analysis of the binding proteins and the up/down-stream genes of the differentially expressed lncRNAs revealed that lncRNAs were principally related to inflammatory response. Meanwhile, we found that the differentially expressed lncRNA TCONS_00058367 might lead to a reduction of phosphorylation of transcription factor p65 (p-p65) in TGEV-infected IPEC-J2 cells by negatively regulating its antisense gene promyelocytic leukemia (PML). CONCLUSIONS: The data showed that differentially expressed lncRNAs might be involved in inflammatory response induced by TGEV through acting as miRNA sponges, regulating their up/down-stream genes, or directly binding proteins.

10.
Math Biosci Eng ; 16(6): 6962-6974, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31698598

RESUMO

To investigate effect of the number of deviators, the tension method, and the tendon profile on the flexural behaviour of reinforcement concrete (RC) T-beams strengthened with externally prestressed tendons, seven identical RC T-beams strengthened with external prestressing tendons were tested under four-point loading. Of these, one beam was ordinary RC beam without strengthening, another six beams were classified into three groups termed G1, G2 and G3. Two beams in G1 had identical straight external tendons with a different number of deviators, two beams in G2 had identical V shape external tendons with different tension method, and two beams in G3 had identical U shape external tendons with different tension method. The failure mode, deflection, strain, load carrying capacity and ductility of the specimens under loading were recorded and analyzed. Test results indicated that strengthening with external prestressing tendons is a very effective method to improve the load carrying capacity and stiffness of the RC beam. Provision of two deviators at the loading points led to satisfactory service load behavior (deflection, cracking, and concrete strain) and a higher load carrying capacity compared to the case where one deviator or no deviators were provided. In addition, tension method of the external tendon nearly had no effect on the load carrying capacity and mechanical behaviour of the RC beams.

11.
Int Immunopharmacol ; : 105960, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31704287

RESUMO

The mTOR signaling pathway has been demonstrated to be related to the development of osteoarthritis (OA) by regulating expression of autophagy regulators. Few studies have shed light on the association of mTOR-pathway-related gene variants with OA risk. Totally 441 OA patients and 533 controls were recruited and their genotypes for mTOR-pathway-related gene variants were determined based on the matrix-assisted laser desorption/ionization time of flight mass spectrometry. Genetic risk scores (GRS) were calculated to evaluate the combined effect of these polymorphisms on OA risk. No significant differences were observed in genotypic and allelic frequencies of AKT rs1130233/REDD1 rs1053639 polymorphisms. However, the mTOR rs1034528 polymorphism was demonstrated to be related to an increased risk of OA, especially among smokers and individuals aged ≥60 years. This single nucleotide polymorphism (SNP) also showed significantly correlation with the Lequesne's index. Similarly, the IRS1 rs1801278 polymorphism increased the risk of OA among smokers, drinkers and individuals aged ≥60 years. A strong positive correlation was found between PTEN rs3830675 polymorphism and OA risk, and this SNP was more frequent in the smokers and drinkers groups. No association was found between IRS1 rs1801278/PTEN rs3830675 polymorphism and OA characteristics. Additionally, there was a strong interaction between genetic factors and lifestyles under the combined models (IRS1 rs1801278/ PTEN rs3830675 polymorphism and smoking/drinking). A high GRS was positively related to an increased risk of OA. In summary, three gene polymorphisms (mTOR [rs1034528], IRS1 [rs1801278] and PTEN [rs3830675]) were found to affect the risk of OA development by regulating the mTOR pathway.

12.
Yonsei Med J ; 60(12): 1187-1194, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31769250

RESUMO

PURPOSE: Adipogenic differentiation of adipose tissue-derived mesenchymal stem cells (AMSCs) is critical to many disease-related disorders, such as obesity and diabetes. Studies have demonstrated that miRNA-138 (miR-138) is closely involved in adipogenesis. However, the mechanisms affected by miR-138 remain unclear. This work aimed to investigate interactions between miR-138 and lipoprotein lipase (LPL), a key lipogenic enzyme, in AMSCs. MATERIALS AND METHODS: Human AMSCs (hAMSCs) isolated from human abdomen tissue were subjected to adipogenic differentiation medium. Quantitative real-time polymerase chain reaction and Western blot assay were applied to measure the expressions of miR-138, LPL, and the two adipogenic transcription factors cytidine-cytidine-adenosine-adenosine-thymidine enhancer binding protein alpha (C/EBPα) and peroxisome proliferator-activated receptor gamma (PPARγ). The relationship between miR-138 and LPL was predicted utilizing the miRTarBase database and validated by dual luciferase reporter assay. RESULTS: Showing increases in C/EBPα and PPARγ expression levels, hAMSCs were induced into adipogenic differentiation. During adipogenesis of hAMSCs, miR-138 expression was significantly downregulated. Overexpression of miR-138 by transfection inhibited hAMSCs adipogenic differentiation in vitro. Mechanically, LPL was a target of miR-138. LPL expression was upregulated during adipogenesis of hAMSCs, and this upregulation was reversed by miR-138 overexpression. Functionally, silencing of LPL by transfection exerted similar inhibition of the expressions of C/EBPα and PPARγ. Meanwhile, LPL ectopic expression was able to partly abolish the suppressive effect of miR-138 overexpression on adipogenic differentiation of hAMSCs. CONCLUSION: Upregulation of miR-138 inhibits adipogenic differentiation of hAMSCs by directly downregulating LPL.

13.
Bioresour Technol ; : 122430, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31761626

RESUMO

The effects on nitrogen removal from landfill leachate were compared between the denitrification (DN) direct coupling in Partial nitrification (PN)-Anammox (DN+(PN-Anammox)) and pre-DN followed by PN-Anammox (DN-PN-Anammox). Both processes can achieve coupling and high nitrogen removal. However, the DN+(PN-Anammox) process was not conducive to the treatment of high-COD wastewater. The total nitrogen removal rate (TNRR) and total nitrogen removal efficiency (TNRE) were stable at 0.31 kg/(m3·d) and 76.3%. When UASB was added to denitrification and transform the process into DN-PN-Anammox, the influent NH4+-N and COD concentrations were increased to 2230 and 2612 mg/L, TNRR and TNRE reached 0.45 kg/(m3·d) and 96.7%, respectively. The DN-PN-Anammox process not only was able to make full use of degradable COD in wastewater to realize the NO3--N removal, but also benefited the growth of autotrophic bacteria. The DN-PN-Anammox reduced the oxygen supply and was more conducive to the treatment of highly-concentrated mature landfill leachate.

14.
Mol Med Rep ; 20(5): 4551-4557, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702026

RESUMO

Increasing evidence has indicated that long noncoding RNAs (lncRNAs) are involved in the tumorigenesis and progression of various types of cancer. The lncRNA deleted in lymphocytic leukemia 1 (DLEU1) has been reported to be dysregulated in cancer cells and thus associated with tumor development; however, the role of DLEU1 in renal cell carcinoma (RCC) remains unclear. In the present study, DLEU1 was knocked down using small interfering RNA in the RCC cell lines KETR3 and 786­O to determine the role of DLEU1. Cell Counting Kit­8, colony formation, Transwell and flow cytometry assays were performed to assess the effects of DLEU1 on cell proliferation, migration, invasion and apoptosis in KETR3 and 786­O cells. The protein expression levels of factors associated with apoptosis and epithelial­mesenchymal transition (EMT) were examined by western blot. The results demonstrated that silencing DLEU1 decreased the growth capacity, migration and invasion of KETR3 and 786­O cells. Additionally, loss of DLEU1 was observed to stimulate the mitochondrial pathway of cell apoptosis via regulation of the expression of Bcl­2/Bax, cleaved caspase­3 and cleaved caspase­9 in KETR3 and 786­O cells. Furthermore, DLEU1 knockdown significantly inhibited the protein kinase B (Akt) pathway by downregulating the expression of phosphorylated­Akt, cyclin  D1 and P70S6 kinase. In addition, depletion of DLEU1 was observed to impair the process of EMT in RCC cells via the upregulation of E­cadherin, and downregulation of N­cadherin and vimentin. Collectively, these results indicated a pro­oncogenic role of DLEU1 in the progression and development of RCC via modulation of the Akt pathway and EMT phenotype.

15.
Ecotoxicol Environ Saf ; : 109903, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31706567

RESUMO

Liming has been widely used to remediate Cd-contaminated acidic soils, but the effects of consecutive liming are still unclear. Four-year liming experiments were conducted to assess the remediation of Cd-contaminated acidic paddy fields in a double rice cropping system. With four-year consecutive liming (quicklime, 2.25 t ha-1 per season), the soil pH was increased by an average of 0.57 units (0.10-1.16 units), while the soil DTPA-Cd and available Fe and Mn were reduced by 9%, 19% and 31% (p < 0.05), respectively. The exchangeable plus water-soluble Cd fraction in soil was reduced by 17%, while the soil carbonate-, Fe/Mn oxide- and organic-bound Cd fractions were increased by 23%, 41% and 10% (p < 0.05), respectively. The Cd in rice grain was reduced by 55% for early rice and 63% for late rice (p < 0.05) and in some cases was reduced to below the Chinese allowable limit (0.2 mg kg-1). High annual fluctuations in rice grain Cd could be caused by variations in the field water regime and in rainfall. With consecutive liming, the soil pH, DTPA-Cd and rice grain Cd varied greatly in the first three seasons and then remained relatively less variable. Meanwhile, soil available nutrients (N, P and K) and rice grain yield were little affected by liming. Soil DTPA-Cd linearly decreased with increasing soil pH, while the reduction of Cd in rice grain logarithmically decreased with increasing soil pH and the reduction in soil DTPA-Cd in the heading stage, indicating potential implications for forecasting rice grain Cd content. Therefore, consecutive liming with quicklime can be recommended for the remediation of Cd-contaminated acidic paddy fields, though supplementary measures are still necessary.

16.
Anal Chim Acta ; 1092: 32-41, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31708030

RESUMO

Deer-hide gelatin (DHG) is an important animal-derived traditional Chinese medicine (TCM), which has been applied in TCM for over 400 years. However, it is extremely difficult to distinguish DHG with adulteration or made with other animal skins due to the highly processing procedure. Therefore, a simple strategy for identifying species-specific peptide biomarkers in deer-hide gelatin (DHG) is needed. In the present study, untargeted and targeted mass spectrometry approaches were implemented to analyze comprehensive peptidomic profiles of trypsin-digested animal gelatins. Mathematics set theory was then used to interrogate the relationship between different samples and peptides in the target species set, while the peptides were not considered as species-specific biomarkers in other sets. Two peptides were identified as DHG-specific peptides. Targeted mass spectrometry approach was then used to verify these two peptides. It showed that these two peptides could be used for distinguishing DHG from other animal hide gelatins. The present strategy provides a simple method for peptide biomarker discovery, which can be applied in the identification of specific peptides in some highly processed animal derived traditional Chinese medicines (TCMs). Thus, the present work provides an effective strategy for rapid, simple discovery and application of species-specific peptide biomarkers to ensure animal derived TCMs quality and make them authenticable and traceable.

17.
Zootaxa ; 4614(2): zootaxa.4614.2.7, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31716383

RESUMO

Two new species of the family Xyalidae from the Laizhou Bay of the Bohai Sea, China are described and illustrated herein. Daptonema papillifera sp. nov. is characterized by relatively small body size, L-shaped spicules with a large cephalate proximal end, triangular gubernaculum with a dorsal apophysis, 5-6 conjoint precloacal cuticularized spines and two ventral papillae located at the middle of the tail. Daptonema papillifera sp. nov. is easily distinguished from the other species in this genus by having 5-6 conjoint precloacal cuticularized spines and two ventral caudal papillae. Pseudosteineria anteramphida sp. nov. is characterized by eight groups of long subcephalic setae located posterior to amphideal fovea, curved slender spicules with cephalate proximal end and tapered distal end, tubular gubernaculum without apophysis, precloacal supplements absent, and a short precloacal seta present. In comparison with its most similar congeneric species, P. ventropapillata Tchesunov, 2000 the new species differs in having smaller body, not jointed cephalic setae, absence of precloacal supplements and absence of gubernacular apophysis.


Assuntos
Nematoides , Animais , Baías , Tamanho Corporal , China , Cromadoria
18.
Zootaxa ; 4695(2): zootaxa.4695.2.10, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31719361

RESUMO

A new free-living marine nematode species from marine sediments in the East China Sea, is described here as Perspiria boucheri sp. nov. The new species is characterized by amphideal fovea surrounded partially by body annulations; four cephalic setae stout; conical buccal cavity with a minute dorsal tooth and two ventrosublateral teeth; pharynx with a pyriform terminal bulb; tail elongated, conical with 3/4 posterior cylindrical portion which having distinct coarse annulations; spicules strongly curved with narrow ventral velum, handle-shaped proximally; gubernaculum canoe-shaped, without apophysis. The new species differs from the most similar species Perspiria striaticaudata (Timm, 1962) by strongly curved spicules with handle-shaped proximal end, gubernaculum without dorsal apophysis and relatively shorter tail. Updated key to all species of Perspiria is provided.


Assuntos
Nematoides , Animais , China , Cromadoria
19.
Opt Express ; 27(20): A1431-A1440, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684496

RESUMO

In this paper, we designed a single sized Metal-Insulator Pair-Metal hybrid grating for dual-band perfect absorption from 8 µm to 14 µm utilizing both nondispersive insulators and dispersive phonic insulators. The hybrid grating was composed of Al/ZnTe-SiC pair/Al, which incorporated an ultrathin phononic SiC layer between the nondispersive ZnTe dielectric spacer and Al substrate. The physical mechanisms responsible for the dual-band perfect absorption were elucidated by the resonance of fundamental magnetic polaritons (MPs). Dual-band perfect absorption with incident angle insensitive feature was enabled. An equivalent LC circuit model predicting the dual-band resonant absorption peaks wavelengths was proposed and verified. Furthermore, the effects of grating period, strip width, nondispersive dielectric spacer thickness and polar phononic dielectric spacer thickness on the absorption were explored.

20.
J Chromatogr A ; : 460693, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31732154

RESUMO

An ultrasensitive microchip electrophoresis chemiluminescence (MCE-CL) assay platform based on separation assisted cascade signal amplification was developed for detection of trace biomolecules. In this work, the aptamer was used as a target probe to bind target molecule and triggering cascade signal amplification reaction. The horseradish peroxide labeled DNA (HRP-DNA) was used as signal probe, utilizing nucleic acid hybridization and exonuclease cutting technology realized ultrasensitive detection of biomolecules on the MCE-CL assay platform. Taking gamma interferon (IFN-γ) as a model analyte, the linear range for IFN-γ detection is 8.0 × 10-15-1.0 × 10-8 M, the detection limit is 1.6 fM, which is six orders magnitude lower than that of without signal amplification. The proposed method was successfully applied for the quantification of IFN-γ in human plasma samples. It was demonstrated that the MCE-CL assay platform was quick, sensitive, and highly selective. It may serve as a tool for clinical analysis of IFN-γ to assist in the diagnosis of disease.

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