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1.
Bioact Mater ; 7: 154-166, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34466724

RESUMO

Zinc is generally considered to be one of the most promising materials to be used in biodegradable implants, and many zinc alloys have been optimized to improve implant biocompatibility, degradation, and mechanical properties. However, long-term degradation leads to the prolonged presence of degradation products, which risks foreign body reactions. Herein, we investigated the in vivo biocompatibility and degradation of a biodegradable Zn-Mg-Fe alloy osteosynthesis system in the frontal bone, mandible, and femur in beagles for 1 year. Results of the routine blood, biochemical, trace element, and histological analyses of multiple organs, peripheral blood CD4/CD8a levels, and serum interleukin 2 and 4 levels showed good biocompatibility of the Zn-Mg-Fe alloy. Zinc content analysis revealed zinc accumulation in adjacent bone tissue, but not in the liver, kidney, and spleen, which was related to the degradation of the Zn-Mg-Fe alloy. The alloy demonstrated a uniform slowing degradation rate in vivo. No degradation differences in the frontal bone, mandible, and femur were observed. The degradation products included zinc oxide [ZnO], zinc hydroxide [Zn(OH)2], hydrozincite [Zn5(OH)6(CO3)2], and hopeite [Zn3(PO4)2·4H2O]. The good biocompatibility and degradation properties of the Zn-Mg-Fe alloy render it a very attractive osteosynthesis system for clinical applications.

2.
J Ethnopharmacol ; 282: 114608, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34517059

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Nelumbo nucifera (N. nucifera), a kind of edible Chinese herbal, has been studied in treating hyperlipidemia. However, the hypolipidemic mechanism of N. nucifera remains unknown. Aims of this review: We aimed to screen the effective constituent of N. nucifera alkaloids and elucidated the potential mechanism for treating hyperlipidemia. A triple combination strategy of UHPLC-MSn, hypolipidemic activity and transcriptome sequencing was built to unveil the hypolipidemic mechanism of Nelumbo nucifera alkaloid. MATERIALS AND METHODS: We comprehensively investigated the characterization of N. nucifera alkaloids by using UHPLC-LTQ-Orbitrap MSn. And the hypolipidemic activity of candidate active ingredients were evaluated on sodium oleate-induced HepG2 cell. Finally, O-nornuciferine and N. nucifera alkaloid extraction were analyzed by RNA sequence (RNA-seq) to decipher the underlying hypolipidemic mechanism and were verified by qRT-PCR. RESULTS: 35 compounds were identified from N. nucifera alkaloid extraction by UHPLC-LTQ-Orbitrap MSn. Among them, O-nornuciferine and N. nucifera alkaloid extraction which showed significant hypolipidemic activity were analyzed by transcriptome sequencing. After the intervention of O-nornuciferine and N. nucifera alkaloid extraction, 1 and 158 differentially expressed genes (DEGs) were identified, severally. The enrichment analysis indicated that the hypolipidemic effect was adjusted by the expression of numerous key DEGs involved in bile secretion, glycerolipid and sphingolipid metabolism, PPAR signaling pathway. CONCLUSIONS: O-nornuciferine and N. nucifera alkaloids had exibited significant effects in hyperlipidemia. The candidate genes were LDLR, LPL and ANGPTL4, etc. It was most likely that they adjusted lipid metabolism by modulating expression levels of various key factors which were involved in bile secretion, glycerolipid metabolism, sphingolipid metabolism and PPAR signaling pathway, and so on. This study clarified the hypolipidemic mechanism of the alkaloids in N. nucifera, and laid a foundation for the subsequent development of clinical application and better quality of N. nucifera.

3.
Food Chem ; 370: 131264, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34788949

RESUMO

Supercooling can preserve beef without freezing damage, whereas maintaining the supercooled state is difficult. An innovative method of static magnetic field extended supercooling (SM-ES) was proposed to maintain the non-frozen state of beef. Effect of SM-ES (-4 °C + SMF) compared with refrigerated (4 °C), slow-frozen (-4 °C) and frozen (-18 °C) treatment on beef quality was investigated. Results demonstrated that SM-ES successfully preserved beef at -4 °C without ice nucleation for 14 days. The SEM images revealed that the microstructure of slow-frozen/frozen samples was damaged due to crystallizing, while the ice nucleation was not observed in SM-ES treated beef. Compared with refrigerated, slow-frozen and frozen treatment, the drip loss of SM-ES decreased by 21.9%, 47.8% and 30.9%, respectively. The lipid oxidation degree of beef decreased following SM-ES treatment. SM-ES treatment extended the shelf-life of beef for more than 6 days compared with refrigeration while prevented its crystallizing.


Assuntos
Metabolismo dos Lipídeos , Refrigeração , Animais , Bovinos , Congelamento , Campos Magnéticos , Oxirredução
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120266, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34391063

RESUMO

In this paper, two tetrahydo[5]helicene-based dyes (THH1 and THH2) were studied on their twist intramolecular charge transfer. And the water-soluble blue carbon dots (N-CDs) were synthesized. Considering that their different optical propeties, the ratiometric fluorescence probes N-CDs/THH1 and N-CDs/THH2 were constructed by mixing N-CDs and dyes simply. It was found that N-CDs/THH1 and N-CDs/THH2 could quickly and sensitively detect water content in ethanol, with linear range of 0.500-25.0 vol% and 0.500-30.0 vol%, respectively. What's more, through the actual sample test, it showed that the detection had good accuracy and precision. At the same time, it was found that two ratiometric probes could also be applied to the thermometry with good reversibility based on optical temperature sensors.


Assuntos
Corantes Fluorescentes , Pontos Quânticos , Etanol , Temperatura , Água
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120306, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34461524

RESUMO

Discrimination of human serum albumin (HSA) from bovine serum albumin (BSA) based on the fluorescence probe technique is still challenging due to similar chemical structures. In this work, a novel flavonoid-based fluorescent probe AF is reported for successful discrimination of HSA from BSA. The sensing performances of probe, including sensing dynamic, sensitivity and selectivity, have been carefully studied. Moreover, sensing mechanism was elucidated by Job's plot, displacement experiment, and molecular docking, suggesting that the specific response to HSA originated from the albumin-induced restricted intramolecular rotation (RIR) of probe. This work may provide a simple way for designing of novel probes for HSA with high selectivity.


Assuntos
Soroalbumina Bovina , Albumina Sérica Humana , Flavonoides , Humanos , Simulação de Acoplamento Molecular , Ligação Proteica , Albumina Sérica Humana/metabolismo , Espectrometria de Fluorescência
7.
J Proteomics ; 250: 104383, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34562664

RESUMO

Lysine 2-hydroxyisobutyrylation (Khib) is one of the newly discovered post-translational modifications (PTMs) through protein acylation. It has been reported to be widely distributed in both eukaryotes and prokaryotes, and plays an important role in chromatin conformation change, gene transcription, protein subcellular localization, protein-protein interaction, signal transduction, and cellular proliferation. In this study, the Khib modification proteome of siliques from A. thaliana under salt stress (Ss) and those in the control (Cs) were compared. The results showed that Khib modification was abundant in siliques. Totally 3810 normalized Khib sites on 1254 proteins were identified, and the Khib modification showed a downregulation trend dramatically: it was down-regulated at 282 sites on 205 proteins while was up-regulated at 96 sites on 78 proteins in Ss siliques (Data are available via ProteomeXchange with identifier PXD028116 and PXD026643). Among them, 13 proteins, including F4IVN6, Q9M1P5, and Q9LF33, had sites with the most significant regulation of Khib modification. Bioinformatics analysis suggested that the differentially Khib-regulated proteins mainly participated in glycolysis/gluconeogenesis and endocytosis. In particular, there were differentially117 Khib-regulated proteins that were mapped to the protein-protein interaction database. In the KEGG pathway enrichment analysis, Khib-modified proteins were enriched in several pathways related to energy metabolism, including gluconeogenesis pathway, pentose phosphate pathway, and pyruvate metabolism. Overall, our work reveals the first systematic analysis of Khib proteome in Arabidopsis siliques under salt stress, and sheds a light on the future studies on the regulatory mechanisms of Khib during the salt stress response of plants. SIGNIFICANCE: In this study, we found the Khib-modified proteins in silique under salt stress and described the enrichment of Khib-modified proteins involved in the biological processes and cellular localization. Proteins undergoing 2-hydroxyisobutylation were mainly involved in the gluconeogenesis pathway, pentose phosphate pathway, and pyruvate metabolism, suggesting that 2-hydroxyisobutylation affects the energy metabolic pathway, and thus the development of the plant. In addition, specific candidate proteins that may affect plant development under salt stress were selected. This study will provide a theoretical basis for revealing the function and mechanism of these proteins and their 2-hydroxyisobutyryl modifications during the development of silique under salt stress.

8.
Carbohydr Polym ; 275: 118691, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34742418

RESUMO

Fucosylated chondroitin sulfates (FCS) are a sulfated polysaccharide exclusively existing in the body wall of sea cucumber. FCS possesses a mammalian chondroitin sulfate like backbone, namely repeating disaccharides units composed of GlcA and GalNAc, with fucosyl branches linked to GlcA and/or GalNAc residues. It is found that FCS can prevent unhealthy dietary pattern-induced metabolic syndromes, including insulin resistance and ß-cell function improvement, anti-inflammation, anti-hyperlipidemia, and anti-adipogenesis. Further studies show that those activities of FCS might be achieved through positively modulating gut microbiota composition. Besides, FCS also show therapeutic efficacy in cancer, HIV infection, and side effects of cyclophosphamide. Furthermore, bioactivities of FCS are closely affected by their molecular weights, sulfation pattern of the fucosyl branches, and chain conformations. This review summarizes the recent 20 years studies to provide references for the future studies and applications of FCS in functional foods or drugs.

9.
Clin Nucl Med ; 2021 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-34739399

RESUMO

ABSTRACT: A 22-year-old man with a history of recurrent papillary thyroid cancer underwent 131I therapy. Posttherapeutic whole-body scintigraphy was performed 5 days after administration of 5.5 GBq of 131I. The scintigraphy revealed increased activity in the left elbow. On physical examination, the patient had posttraumatic superficial scab at the site of the abnormal activity.

10.
Chemosphere ; : 132745, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34743800

RESUMO

With the acceleration of urbanization and the improvement of people's living standards, more chemicals that humans rely on are entering the city and surrounding water bodies. Anionic surfactants are one of the essential products for human beings. It is also one of the inducements that cause the eutrophication. The algae-laden water caused by eutrophication is a headache in the traditional water treatment process. To solve the problem, ultrafitration combined process was widely investigated to treat the algae-laden water. The presence of stimuli, low concentration anionic surfactant, probably interfere the performance of ultrafiltration process during algae-laden water treatment. In this study, the influence of two typical anionic surfactants, sodium dodecyl sulfate (SDS) and sodium dodecyl benzene sulfonate (LAS), on the performance of coagulation-enhanced ultrafiltration was investigated. The aluminum sulfate hydrate and iron sulfate hydrate were respectively employed as coagulant. Based on the residual turbidity and zeta potential, 4 mg/L Al and 8 mg/L Fe were determined as the optimal coagulant dosage. The floc morphology confirmed that Al-algae flocs with lower fractal dimension (Df) were looser and more porous compared to Fe-algae flocs. More coagulant was depleted by LAS due to the better hydrophobicity of LAS. During the filtration process, LAS caused a larger flux reduction compared with SDS regardless of the coagulant that was used. More organic compounds penetrate into membrane pores and block the pores with the presence of LAS since algal cell aggregation was weakened. Finally, the rejection of organic compounds by the coagulation-enhanced ultrafiltration process was studied, and the co-existing surfactants can cause effluent deterioration. Therefore, the presence of surfactants has a negative effect to the ultrafiltration treatment of algae-laden water.

11.
Cell Cycle ; : 1-18, 2021 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-34747340

RESUMO

This study explored the effects of miR-125-5p and interleukin-6 receptor (IL-6 R) on ulcerative colitis (UC) cell models and mouse models. The sera derived from UC patients and healthy subjects were collected for expression analysis. UC in vitro models and in vivo model were constructed and used. Expressions of miR-125-5p, IL-6 R, AK1/STAT3 and NF-κB pathways, and inflammatory factors, histopathology and apoptosis were determined by conducting a series of molecular experiments. The relationship between miR-125-5p and IL-6 R was analyzed by TargetScan7.2 and verified by dual-luciferase assay. The disease activity index (DAI) score, weight change, and colon length of the mice were recorded and analyzed. Decreased expression of miR-125-5p in the sera of UC patients was related to the increased expression of its target gene IL-6 R. In vitro, up-regulation of miR-125-5p decreased IL-6 R expression, contents of inflammatory factors in THP-1 cells and cell apoptosis of NCM460, and inhibited the activation of JAK1/STAT3 and NF-κB pathway. However, down-regulation of miR-125-5p produced the opposite effects to its up-regulation. IL-6 R overexpression partially reversed the effects of miR-125-5p up-regulation on UC cell models. In vivo, miR-125-5p overexpression significantly improved the severity of colitis, including DAI score, colon length, tissue damage, apoptosis, and inflammatory response, in the mice in the UC group. In addition, miR-125-5p up-regulation significantly reduced the expression of IL-6 R in the UC mice, and reduced the expression levels of JAK1, STAT3 and p65 phosphorylation. MiR-125-5p targeting IL-6 R regulates macrophage inflammatory response and intestinal epithelial cell apoptosis in ulcerative colitis through JAK1/STAT3 and NF-κB pathway.

12.
Front Plant Sci ; 12: 738042, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34745172

RESUMO

Real-time dynamic monitoring of orchard grape leaf diseases can greatly improve the efficiency of disease control and is of great significance to the healthy and stable development of the grape industry. Traditional manual disease-monitoring methods are inefficient, labor-intensive, and ineffective. Therefore, an efficient method is urgently needed for real-time dynamic monitoring of orchard grape diseases. The classical deep learning network can achieve high accuracy in recognizing grape leaf diseases; however, the large amount of model parameters requires huge computing resources, and it is difficult to deploy to actual application scenarios. To solve the above problems, a cross-channel interactive attention mechanism-based lightweight model (ECA-SNet) is proposed. First, based on 6,867 collected images of five common leaf diseases of measles, black rot, downy mildew, leaf blight, powdery mildew, and healthy leaves, image augmentation techniques are used to construct the training, validation, and test set. Then, with ShuffleNet-v2 as the backbone, an efficient channel attention strategy is introduced to strengthen the ability of the model for extracting fine-grained lesion features. Ultimately, the efficient lightweight model ECA-SNet is obtained by further simplifying the network layer structure. The model parameters amount of ECA-SNet 0.5× is only 24.6% of ShuffleNet-v2 1.0×, but the recognition accuracy is increased by 3.66 percentage points to 98.86%, and FLOPs are only 37.4 M, which means the performance is significantly better than other commonly used lightweight methods. Although the similarity of fine-grained features of different diseases image is relatively high, the average F1-score of the proposed lightweight model can still reach 0.988, which means the model has strong stability and anti-interference ability. The results show that the lightweight attention mechanism model proposed in this paper can efficiently use image fine-grained information to diagnose orchard grape leaf diseases at a low computing cost.

14.
Biomacromolecules ; 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34762396

RESUMO

Oxidation-sensitive drug delivery systems (DDSs) have attracted attention due to the potential to improve efficacy and safety of chemotherapeutics. These systems are designed to release the payload in response to oxidative stress conditions, which are associated with many types of cancer. Despite extensive research on the development of oxidation-sensitive DDS, the lack of selectivity toward cancer cells over healthy cells remains a challenge. Here, we report the design and characterization of polymeric micelles containing thioether groups with varying oxidation sensitivities within the micellar core, which become hydrophilic upon thioether oxidation, leading to destabilization of the micellar structure. We first used the thioether model compounds, 3-methylthiopropylamide (TPAM), thiomorpholine amide (TMAM), and 4-(methylthio)benzylamide (TPhAM) to investigate the effect of the chemical structures of the thioethers on the oxidation by hydrogen peroxide (H2O2). TPAM shows the fastest oxidation, followed by TMAM and TPhAM, showing that the oxidation reaction of thioethers can be modulated by changing the substituent groups bound to the sulfur atom. We next prepared micelles containing these different thioether groups within the core (TP, TM, and TPh micelles). The micelles containing the thioether groups with a higher oxidation sensitivity were destabilized by H2O2 at a lower concentration. Micelle destabilization was also tested in human liver cancer (HepG2) cells and human umbilical vein endothelial cells (HUVECs). The TP micelles having the highest oxidation sensitivity were destabilized in both HepG2 cells and HUVECs, while the TPh micelles, which showed the lowest reactivity toward H2O2, were stable in these cell lines. The TM micelles possessing a moderate oxidation sensitivity were destabilized in HepG2 cells but were stable in HUVECs. Furthermore, the micelles were loaded with doxorubicin (Dox) to evaluate their potential in drug delivery applications. Among the micelles, the TM micelles loaded with Dox showed the enhanced relative toxicity in HepG2 cells over HUVECs. Therefore, our approach to fine-tune the oxidation sensitivity of the micelles has potential for improving therapeutic efficacy and safety of drugs in cancer treatment.

15.
Front Pharmacol ; 12: 755077, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34764873

RESUMO

Right ventricular (RV) remodeling is one of the essential pathological features in pulmonary arterial hypertension (PAH). RV hypertrophy or fibrosis are the leading causes of RV remodeling. Magnolol (6, 6', 7, 12-tetramethoxy-2,2'-dimethyl-1-ß-berbaman, C18H18O2) is a compound isolated from Magnolia Officinalis. It possesses multiple pharmacological activities, such as anti-oxidation and anti-inflammation. This study aims to evaluate the effects and underlying mechanisms of magnolol on RV remodeling in hypoxia-induced PAH. In vivo, male Sprague Dawley rats were exposed to 10% O2 for 4 weeks to establish an RV remodeling model, which showed hypertrophic and fibrotic features (increases of Fulton index, cellular size, hypertrophic and fibrotic marker expression), accompanied by an elevation in phosphorylation levels of JAK2 and STAT3; these changes were attenuated by treating with magnolol. In vitro, the cultured H9c2 cells or cardiac fibroblasts were exposed to 3% O2 for 48 h to induce hypertrophy or fibrosis, which showed hypertrophic (increases in cellular size as well as the expression of ANP and BNP) or fibrotic features (increases in the expression of collagen Ⅰ, collagen Ⅲ, and α-SMA). Administration of magnolol and TG-101348 or JSI-124 (both JAK2 selective inhibitors) could prevent myocardial hypertrophy and fibrosis, accompanied by the decrease in the phosphorylation level of JAK2 and STAT3. Based on these observations, we conclude that magnolol can attenuate RV hypertrophy and fibrosis in hypoxia-induced PAH rats through a mechanism involving inhibition of the JAK2/STAT3 signaling pathway. Magnolol may possess the potential clinical value for PAH therapy.

16.
Prev Sci ; 2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34719736

RESUMO

This study aimed to examine the relationship between electronic cigarette use and subsequent combustible cigarette use, controlling for confounding by using a propensity score method approach. Data from the first three annual waves of the Population Assessment of Tobacco and Health study were analyzed (n = 6309). Participants were tobacco-naïve at Wave 1; used e-cigarettes exclusively (n = 414), used combustible cigarettes exclusively (n = 46), or not used any tobacco products (n = 5849) at Wave 2. We conducted entropy balancing propensity score analysis to examine the association between exclusive e-cigarette or cigarette initiation and subsequent cigarette use at Wave 3, adjusting for non-response bias, sampling bias, and confounding. Among tobacco-naïve youth, exclusive e-cigarette use was associated with greater risk for subsequent combustible cigarette smoking initiation (OR = 3.42, 95% CI = (1.99, 5.93)) and past 30-day combustible cigarette use (OR = 2.88, 95% CI = (1.22, 6.86)) in the following year. However, the latter risk was comparatively lower than the risk if youth started with a combustible cigarette (OR = 25.79, 95% CI = (9.68, 68.72)). Results of sensitivity analyses indicated that estimated effects were robust to unmeasured confounding. Use of e-cigarettes in tobacco-naïve youth is associated with increased risk of subsequent past 30-day combustible cigarette use but the risk is an order of magnitude higher if they start with a combustible cigarette.

17.
Adv Sci (Weinh) ; : e2102886, 2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34719862

RESUMO

Electrochemical CO2 reduction to value-added chemicals/fuels provides a promising way to mitigate CO2 emission and alleviate energy shortage. CO2 -to-CO conversion involves only two-electron/proton transfer and thus is kinetically fast. Among the various developed CO2 -to-CO reduction electrocatalysts, transition metal/N-doped carbon (M-N-C) catalysts are attractive due to their low cost and high activity. In this work, recent progress on the development of M-N-C catalysts for electrochemical CO2 -to-CO conversion is reviewed in detail. The regulation of the active sites in M-N-C catalysts and their related adjustable electrocatalytic CO2 reduction performance is discussed. A visual performance comparison of M-N-C catalysts for CO2 reduction reaction (CO2 RR) reported over the recent years is given, which suggests that Ni and Fe-N-C catalysts are the most promising candidates for large-scale reduction of CO2 to produce CO. Finally, outlooks and challenges are proposed for future research of CO2 -to-CO conversion.

18.
J Healthc Eng ; 2021: 6024352, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34754409

RESUMO

Circle of Willis (CoW) is the most critical collateral pathway that supports the redistribution of blood supply in the brain. The variation of CoW is closely correlated with cerebral hemodynamic and cerebral vessel-related diseases. But what is responsible for CoW variation remains unclear. Moreover, the visual evaluation for CoW variation is highly time-consuming. In the present study, based on the computer tomography angiography (CTA) dataset from 255 patients, the correlation between the CoW variations with age, gender, and cerebral or cervical artery stenosis was investigated. A multitask convolutional neural network (CNN) was used to segment cerebral arteries automatically. The results showed the prevalence of variation of the anterior communicating artery (Aco) was higher in the normal senior group than in the normal young group and in females than in males. The changes in the prevalence of variations of individual segments were not demonstrated in the population with stenosis of the afferent and efferent arteries, so the critical factors for variation are related to genetic or physiological factors rather than pathological lesions. Using the multitask CNN model, complete cerebral and cervical arteries could be segmented and reconstructed in 120 seconds, and an average Dice coefficient of 78.2% was achieved. The segmentation accuracy for precommunicating part of anterior cerebral artery and posterior cerebral artery, the posterior communicating arteries, and Aco in CoW was 100%, 99.2%, 94%, and 69%, respectively. Artificial intelligence (AI) can be considered as an adjunct tool for detecting the CoW, particularly related to reducing workload and improving the accuracy of the visual evaluation. The study will serve as a basis for the following research to determine an individual's risk of stroke with the aid of AI.

19.
PLoS Pathog ; 17(11): e1010070, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34788350

RESUMO

Nuclear scaffold attachment factor A (SAFA) is a novel RNA sensor involved in sensing viral RNA in the nucleus and mediating antiviral immunity. Severe fever with thrombocytopenia syndrome virus (SFTSV) is a bunyavirus that causes SFTS with a high fatality rate of up to 30%. It remains elusive whether and how cytoplasmic SFTSV can be sensed by the RNA sensor SAFA. Here, we demonstrated that SAFA was able to detect SFTSV infection and mediate antiviral interferon and inflammatory responses. Transcription and expression levels of SAFA were strikingly upregulated under SFTSV infection. SAFA was retained in the cytoplasm by interaction with SFTSV nucleocapsid protein (NP). Importantly, SFTSV genomic RNA was recognized by cytoplasmic SAFA, which recruited and promoted activation of the STING-TBK1 signaling axis against SFTSV infection. Of note, the nuclear localization signal (NLS) domain of SAFA was important for interaction with SFTSV NP and recognition of SFTSV RNA in the cytoplasm. In conclusion, our study reveals a novel antiviral mechanism in which SAFA functions as a novel cytoplasmic RNA sensor that directly recognizes RNA virus SFTSV and mediates an antiviral response.

20.
Materials (Basel) ; 14(21)2021 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-34771861

RESUMO

Owing to their extreme hardness, cubic boron nitride (cBN) composites are widely used in cutting applications. The performance of cBN composites is closely related to the characteristics of the binder. Therefore, novel binders must be developed to improve the performance of cBN composites. In the present work, TiAl intermetallics were used as binders to fabricate cBN composites by employing a high-temperature and high-pressure sintering method. The phase transformation, sintering reaction mechanism, thermal stability, and mechanical properties of the resultant cBN composites were investigated. It was found that during the sintering process, Ti atoms preferentially reacted with boron nitride particles, whereas Al atoms enriched and transformed into TiAl3 phases and formed cBN/AlN, AlB2/TiN, and TiB2/TiAl3-layered structures eventually. The composites maintained good oxidation resistance at 1200 °C. A decrease in the particle size of the TiAl binder improved the uniformity of particle size distribution and increased the flexural strength of the composites.

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