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1.
Cancer Lett ; : 216075, 2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36736530

RESUMO

N6-methyladenosine (m6A) RNA methylation and its associated RNA-binding protein insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) are involved in tumor initiation and progression. Here, we explored the biological function and clinical significance of IGF2BP1 in intrahepatic cholangiocarcinoma (iCCA). We found that IGF2BP1 expression was upregulated by H3K27 acetylation enrichment of its promoter, which positively correlated with poor clinicopathological characteristics and survival. Gain- and loss-of-function experiments showed that IGF2BP1 overexpression (knockdown) enhanced (attenuated) iCCA growth and metastasis in vitro and in vivo. Mechanistically, IGF2BP1 not only regulated the c-Myc/p16 axis to promote iCCA growth and inhibit senescence, but also activated the ZIC2/PAK4/AKT/MMP2 axis to induce tumor metastasis. More importantly, BTYNB, a recently identified IGF2BP1 inhibitor, exerted promising anti-tumor efficacy in a patient-derived xenograft (PDX) model, and IGF2BP1 conditional knockout (cKO) reduced the tumor burden. These results demonstrate the crucial role of IGF2BP1 in iCCA progression via m6A-dependent modification, highlighting IGF2BP1 as a potential therapeutic target in iCCA.

2.
Phys Chem Chem Phys ; 2023 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-36734476

RESUMO

The Raman intensity and other stoichiometric calculations of nitromethane (NM) and 2-nitrimino-5-nitro-hexahydro-1,3,5-triazine (NNHT) have been made by using first-principles density functional theory. We propose a method to judge the initial reaction mechanism of NM and NNHT under pressure based on the Raman intensity. Both the resulting NM and NNHT undergo hydrogen transfer and conventional trigger bond cleavage. And the results obtained from the Raman peak intensities infer a reaction path that is not inferior to the traditional C-NO2 and N-NO2 bond cleavage, thus verifying our results.

3.
Phys Chem Chem Phys ; 2023 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-36727537

RESUMO

Understanding and predicting the bond breaking mechanism of energetic materials before explosion initiation is one of the huge challenges in explosion science. By means of the mean square displacement of the atom from the equilibrium position and theoretical bond breaking tensile change of the chemical bond, we establish a new criterion to judge whether the chemical bond is broken. Further, α-RDX is used as the verification object to verify the accuracy of this model. We obtained an initial decomposition temperature of 434-513 K for α-RDX at 0 GPa, and the initial bond breaking type was N-NO2. Finally, based on this model, we discussed in detail the breaking of chemical bonds of solid nitromethane near the detonation pressure. We think that the high temperature and high pressure caused by the shock wave may break all the chemical bonds of nitromethane near the detonation pressure.

4.
Artigo em Inglês | MEDLINE | ID: mdl-36723949

RESUMO

Layered O3-type transition metal oxides are promising cathode candidates for high-energy-density Li-ion batteries. However, the structural instability at the highly delithiated state and low kinetics at the fully lithiated state are arduous challenges to overcome. Here, a facile approach is developed to make secondary particles of Ni-rich materials with nanosheet primary grains. Because the alignment of the primary grains reduces internal stress buildup within the particle during charge-discharge and provides straightforward paths for Li transport, the as-synthesized Ni-rich materials do not undergo cracking upon cycling with higher overall Li+ ion diffusion rates. Specifically, a LiNi0.75Co0.14Mn0.11O2 cathode with nanosheet grains delivers a high reversible capacity of 206 mAh g-1 and shows ultrahigh cycling stability, e.g., 98% capacity retention over 500 cycles in a full cell with a graphite anode.

5.
J Colloid Interface Sci ; 637: 67-75, 2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36682119

RESUMO

Traditional self-polishing antifouling coatings kill surface organisms by releasing toxic substances, which are damaging to the ecosystem. As a natural antimicrobial substance, eugenol is environmentally friendly and has been proven by different research teams to be effective in enhancing the anti-fouling effect of coatings in the real sea. While in these previous research works, the eugenol was released directly into the seawater thus cannot further serve as surface antifouling effect, leading to a limited antifouling effect of the coating. In this work, the quaternary ammonium component was introduced into the butyl ester-based resin - poly (eugenol methacrylate - acryloyloxyethyltrimethyl ammonium chloride - hexafluorobutyl methacrylate - methyl methacrylate - butyl methacrylate - ethylene glycol methyl ether acrylate) (EMQFP) coating for the first time by simple one-step free radical polymerization method. On the one hand, the eugenol produced by hydrolysis is anchored to the quaternary ammonium on the coating surface for a period of time due to the cationic-π interaction, instead of being released into seawater immediately after hydrolysis, thus increasing the utilization rate of eugenol; on the other hand, the negatively charged carboxylate groups generated after hydrolysis in the coating are mutually attracted to quaternary ammonium through electrostatic effect, so the resin chain segment conformation on the coating surface adjusted to produce zwitterionic-like structure, and the hydration of zwitterionic inhibits primary fouling adhesion.

6.
Dalton Trans ; 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36688505

RESUMO

Developing new high-performance electrode materials for improving the energy density of supercapacitors is an important task. Herein, a new three-dimensional (3D) metal-orgainc framework (MOF) [Mn(BGPD)(H2O)2] (Mn-BGPD; BGPD = N,N'-bis(glycinyl)pyromellitic diimide) was synthesized. When Mn-BGPD is used as the electrode material of supercapacitors, in a three-electrode setup, it shows an outstanding specific capacitance of 832.6 F g-1 at a current density of 1 A g-1. The asymmetrical supercapacitor of Mn-BGPD shows an attractive specific capacitance of 100 F g-1 at 1 A g-1, which corresponds to an excellent energy density of 35.5 W h kg-1. Moreover, better cycling stability with a capacitance retention of 46.7% is also shown. The high electrochemical performance makes Mn-BGPD a very promising electrode material for supercapacitors.

7.
J Mol Model ; 29(2): 43, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36653549

RESUMO

CONTEXT AND RESULTS: 6-Azido-8-nitrotetrazolo[1,5-b]pyridazine-7-amine (3at) is a promising green energetic material, which meets the development requirements of environment-friendly explosives. By discussing the relationship between lattice parameters and pressure, it is found that the compression ratio indicates anisotropy of compressibility. And bond lengths get shorter under pressure, resulting in stronger intermolecular bonds. The N3 group rotates under pressure. And then, the optical properties basically change regularly with the change of pressure. As the pressure increases, the absorption range widens. In the low energy interval, it shows transparency, and then with the increase of energy and pressure, it shows better optical activity. With the increase of pressure and energy, the absorption coefficient increases, representing that the optical activity becomes high. Finally, according to the analysis of mechanical properties, 3at exhibited brittle behavior at 0 GPa and 100 GPa, while at 10 to 90 GPa, the values of ν and B/G are malleable. COMPUTATIONAL AND THEORETICAL TECHNIQUES: Based on density functional theory, the crystal parameters, electronic properties, optical properties, and elastic and mechanical properties of 3at under different pressures were studied theoretically. The GGA-PW91+OBS method was used to calculate the physical parameters under pressure, such as lattice parameters, energy band structures, dielectric function, refractive index, absorption coefficient, and elastic constants. Physical properties under (3at) pressure are predicted.

8.
Intensive Care Res ; : 1-12, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36687387

RESUMO

Purpose: Significant results of randomized controlled trials (RCTs) should be properly weighed. This study adopted fragility index (FI) to evaluate the robustness of significant dichotomous outcomes from RCTs on coronavirus disease 2019 (COVID-19) treatment. Materials and methods: ClinicalTrials.gov and PubMed were searched from inception to July 31, 2021. FIs were calculated and their distribution was depicted. FI's categorical influential factors were analyzed. Spearman correlation coefficient (r s) was reported for the relationship between FI and the continuous characteristics of RCTs. Results: Fifty RCTs with 120 outcomes in 7869 patients were included. The FI distribution was abnormal with median 3 (interquartile range 1-7, P = 0.0001). The FIs and robustness were affected by the outcomes of interest, various patient populations, and interventions (T = 18.215,16.667, 23.107; P = 0.02,0.0001, 0.001, respectively). A cubic relationship between the FIs and absolute difference of events between groups with R square of 0.848 (T = 215.828, P = 0.0001, R square = 0.865) was observed. A strong negative logarithmic relationship existed between FI and the P value with R square = - 0.834. Conclusion: The robustness of significant dichotomous outcomes of COVID-19 treatments was fragile and affected by the outcomes of interest, patients, interventions, P value, and absolute difference of events between the groups. FI was an useful quantitative metric for the binary significant outcomes on COVID-19 treatments. Registration: PROSPERO (CRD42021272455). Supplementary Information: The online version contains supplementary material available at 10.1007/s44231-022-00027-y.

9.
Artigo em Inglês | MEDLINE | ID: mdl-36622818

RESUMO

RATIONALE AND OBJECTIVES: Up to 20% of idiopathic interstitial lung disease is familial, referred to as Familial Pulmonary Fibrosis (FPF). We performed an integrated analysis of FPF genetic risk by comprehensively evaluating for genetic rare variants (RVs) in a large cohort of FPF kindreds. METHODS: We performed whole-exome sequencing and/or candidate gene sequencing from affected individuals in 569 FPF kindreds, followed by co-segregation analysis in large kindreds, gene burden analysis, gene-based risk scoring, cell type enrichment analysis, and co-expression network construction. MEASUREMENTS AND MAIN RESULTS: We found that 14.9% - 23.4% of genetic risk in kindreds could be explained by RVs in genes previously linked to FPF, predominantly telomere related genes. We identified new candidate genes in a small number of families, including SYDE1, SERPINB8, GPR87, and NETO1, and developed tools for evaluation and prioritization of RV-containing genes across kindreds. Several pathways were enriched for RV-containing genes in FPF, including focal adhesion and mitochondrial complex I assembly. By combining single cell transcriptomics with prioritized candidate genes, we discovered that expression of RV-containing genes was enriched in smooth muscle cells, type II alveolar epithelial cells, and endothelial cells. CONCLUSIONS: In the most comprehensive FPF genetic study to date, we defined the prevalence of RVs in known FPF-related genes and identified new candidate genes and pathways relevant to FPF. However, we did not identify new RV-containing genes shared across multiple kindreds, thereby suggesting that heterogeneous genetic variants involving a variety of genes and pathways mediate genetic risk in most FPF kindreds.

10.
Signal Transduct Target Ther ; 8(1): 3, 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36588101

RESUMO

Autism spectrum disorder (ASD) affects 1-2% of all children and poses a great social and economic challenge for the globe. As a highly heterogeneous neurodevelopmental disorder, the development of its treatment is extremely challenging. Multiple pathways have been linked to the pathogenesis of ASD, including signaling involved in synaptic function, oxytocinergic activities, immune homeostasis, chromatin modifications, and mitochondrial functions. Here, we identify secretagogin (SCGN), a regulator of synaptic transmission, as a new risk gene for ASD. Two heterozygous loss-of-function mutations in SCGN are presented in ASD probands. Deletion of Scgn in zebrafish or mice leads to autism-like behaviors and impairs brain development. Mechanistically, Scgn deficiency disrupts the oxytocin signaling and abnormally activates inflammation in both animal models. Both ASD probands carrying Scgn mutations also show reduced oxytocin levels. Importantly, we demonstrate that the administration of oxytocin and anti-inflammatory drugs can attenuate ASD-associated defects caused by SCGN deficiency. Altogether, we identify a convergence between a potential autism genetic risk factor SCGN, and the pathological deregulation in oxytocinergic signaling and immune responses, providing potential treatment for ASD patients suffering from SCGN deficiency. Our study also indicates that it is critical to identify and stratify ASD patient populations based on their disease mechanisms, which could greatly enhance therapeutic success.


Assuntos
Transtorno do Espectro Autista , Secretagoginas , Animais , Camundongos , Transtorno do Espectro Autista/genética , Transtorno do Espectro Autista/metabolismo , Ocitocina/genética , Ocitocina/metabolismo , Fatores de Risco , Secretagoginas/genética , Secretagoginas/metabolismo , Peixe-Zebra/metabolismo , Humanos
11.
Molecules ; 28(2)2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36677668

RESUMO

Many studies have shown that alterations in the gut microbiota are associated with hypertension. Our study aimed to observe the characteristics of the gut microbiota in hypertension and to further explore whether drug molecules can play a therapeutic role in hypertension by interfering with the gut microbiota. We evaluated the differences in the composition of the gut microbiota in spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY). Meanwhile, three first-line cardiovascular disease (CVD) drugs, losartan, atorvastatin, and aspirin, were used to treat the SHR in order to observe their effects on the gut microbiota in SHR. The 16S rDNA results showed that the diversity and richness of the gut microbiota in SHR were significantly reduced compared with that of the WKY, the Firmicutes/Bacteroidetes ratio was increased, the abundances of Bifidobacterium and short chain fatty acids (SCFAs)-producing bacteria decreased, and the abundance of lactate-producing bacteria increased. In addition to lowering the blood pressure, losartan increased the abundances of Alistipes, Bacteroides, and Butyricimonas in SHR, reduced the abundances of Ruminococcaceae, Streptococcus, and Turicibacter, reduced the Firmicutes/Bacteroidetes ratio, and rebalanced the gut microbiota. Losartan also increased the abundances of Bifidobacterium and SCFAs-producing bacteria and reduced the abundance of lactate-producing bacteria. However, atorvastatin and aspirin had no significant effect on the gut microbiota in SHR. The above results showed that losartan could change the characteristics of the gut microbiota in hypertension and rebalance the gut microbiota, which may be related to lowering the blood pressure. Atorvastatin and aspirin have no significant influence on the gut microbiota in SHR.


Assuntos
Microbioma Gastrointestinal , Hipertensão , Ratos , Animais , Losartan/farmacologia , Pressão Sanguínea , Ratos Endogâmicos SHR , Atorvastatina/farmacologia , Aspirina/farmacologia , Hipertensão/tratamento farmacológico , Ratos Endogâmicos WKY
12.
Medicine (Baltimore) ; 102(3): e32654, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36701699

RESUMO

INTRODUCTION: Klebsiella pneumoniae-induced liver abscess (KP-PLA) is a common type of pyogenic liver abscess, severe acute pancreatitis (SAP) has high mortality, and poor prognosis in advanced colon cancer. There have been no report of SAP complicated with colon cancer after KP-PLA as so far. In this study, we reported a case of SAP secondary to KP-PLA with colon cancer for the first time, so as to provide reference for clinical diagnosis and treatment of these diseases. PATIENT CONCERNS AND DIAGNOSIS: A 64-year-old woman with a history of diabetes visited our hospital with abdominal pain for 5 + days. He was diagnosed with KP-PLA a month ago, which had not healed when he was admitted. He was diagnosed with SAP, and histological examination of colonic biopsy confirmed the diagnosis of moderately differentiated adenocarcinoma. INTERVENTIONS AND OUTCOMES: He was treated with intravenous antibiotics and underwent modified endoscopic mucosal resection under colonoscopy. We conducted a 2-month follow-up, and there was no recurrence of liver abscess and pancreatitis. CONCLUSION: Screening for intestinal tumors is necessary in patients with cryptogenic liver abscess, especially KP-PLA with diabetes.


Assuntos
Neoplasias do Colo , Abscesso Hepático Piogênico , Neoplasias Primárias Desconhecidas , Pancreatite , Feminino , Humanos , Pessoa de Meia-Idade , Doença Aguda , Neoplasias do Colo/complicações , Diabetes Mellitus/epidemiologia , Klebsiella pneumoniae/patogenicidade , Abscesso Hepático Piogênico/complicações , Abscesso Hepático Piogênico/microbiologia , Neoplasias Primárias Desconhecidas/complicações , Pancreatite/diagnóstico , Estudos Retrospectivos
13.
Chemosphere ; 314: 137700, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36587916

RESUMO

Microbes serve as important components of ecosystem services and biogeochemical processes in plateau lakes. However, the features of microbiota assembly, abundance and diversity in plateau lakes remain unclear. The microbial communities in surface water from nine plateau lakes in Yunnan Plateau, southwestern China, in the dry and rainy seasons were explored using 16S rRNA gene and 18S rRNA gene sequencing. The results showed that the bacterial community compositions were homogeneous while those of micro-eukaryotes were heterogeneous in plateau lakes. In both seasons, the bacterial phyla of Proteobacteria and Actinobacteriota predominated in oligotrophic lakes. The mesotrophic lakes were dominated by Proteobacteria, Actinobacteriota, Bacteroidota and Cyanobacteria. The eutrophic lakes were mainly dominated by Proteobacteria, Actinobacteriota and Cyanobacteria. The phylum SAR_k_norank had the major micro-eukaryotes in these plateau lakes. The alpha-diversity of bacteria declined in the rainy season, while that of micro-eukaryotes varied from lake to lake. The drivers of microbiotic community assembly in the dry season were identified as nutrient level-related factors. In the rainy season, however, the microbiota in oligotrophic lakes were related to nutrient levels. Microbial communities were driven by precipitation in mesotrophic and eutrophic lakes with large water volumes, while those in lakes with small water volumes were regulated by nutrient level-related factors. Our findings pose first and unique insights into the microbiota of the nine plateau lakes in Yunnan Plateau, providing important ecological knowledge for these lakes with different characteristics.


Assuntos
Cianobactérias , Microbiota , Lagos/química , China , RNA Ribossômico 16S/genética , Cianobactérias/genética , Proteobactérias/genética , Água
14.
Clin Chim Acta ; : 117235, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36716909

RESUMO

Early and differential diagnosis of perihilar cholangiocarcinoma (PHCCA) is highly challenging. This study aimed to evaluate whether volatile organic compounds (VOCs) in bile samples could be emerging diagnostic biomarkers for PHCCA. We collected 200 bile samples from patients with PHCCA and benign biliary diseases (BBD), including a 140-patient training cohort and an 80-patient test cohort. Gas chromatography-ion mobility spectrometry (GC-IMS) was used for VOCs detection. The predictive models were constructed using machine learning algorithms. Our analysis detected 19 VOC substances using GC-IMS in the bile samples and resulted in the identification of three new VOCs, 2-methoxyfuran, propyl isovalerate, and diethyl malonate that were found in bile. Unsupervised hierarchical clustering analysis supported that VOCs detected in the bile could distinguish PHCCA from BBD. Twelve VOCs defined according to 32 signal peaks had significant statistical significance between BBD and PHCCA, including four up-regulated VOCs in PHCCA, such as 2-ethyl-1-hexanol, propyl isovalerate, cyclohexanone, and acetophenone, while the rest eight VOCs were down-regulated. ROC curve analysis revealed that machine learning models based on VOCs could help diagnosing PHCCA. Among them, SVM provided the highest AUC of 0·966, with a sensitivity and specificity of 93·1% and 100%, respectively. The diagnostic model based on different VOC spectra could be a feasible method for the differential diagnosis of PHCCA.

15.
World Neurosurg ; 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36608796

RESUMO

OBJECTIVE: To evaluate the stability and function of the C1-C2 joint after ectopic functional reconstruction (EFR) of the C1 transverse ligament. METHODS: Eight human cadaveric cervical spines (C0-C4) were subjected to in vitro biomechanical test with moment control. Spine specimens were tested under the following conditions: 1) left intact; 2) destabilized by severing the transverse ligament of atlas; 3) after EFR of the transverse ligament. Range of motion was measured in flexion, extension, lateral bending, and axial rotation. RESULTS: Destabilization significantly increased range of motion in all directions compared with the intact status (P < 0.001). However, after EFR of the transverse ligament, range of motion in all directions was restored to the intact state. Meanwhile, coupling motions were reproduced in the axial rotation. CONCLUSIONS: EFR of the transverse ligament virtually recovers all the physiological functions of the native transverse ligament and might be a promising alternative for the treatment of anterior atlantoaxial dislocation. Further studies are warranted before clinical application of EFR of the transverse ligament.

16.
Mol Cell ; 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36630954

RESUMO

Genetic models suggested that SMARCA5 was required for DNA-templated events including transcription, DNA replication, and DNA repair. We engineered a degron tag into the endogenous alleles of SMARCA5, a catalytic component of the imitation switch complexes in three different human cell lines to define the effects of rapid degradation of this key regulator. Degradation of SMARCA5 was associated with a rapid increase in global nucleosome repeat length, which may allow greater chromatin compaction. However, there were few changes in nascent transcription within the first 6 h of degradation. Nevertheless, we demonstrated a requirement for SMARCA5 to control nucleosome repeat length at G1/S and during the S phase. SMARCA5 co-localized with CTCF and H2A.Z, and we found a rapid loss of CTCF DNA binding and disruption of nucleosomal phasing around CTCF binding sites. This spatiotemporal analysis indicates that SMARCA5 is continuously required for maintaining nucleosomal spacing.

17.
Eur Radiol ; 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36600121

RESUMO

OBJECTIVES: To measure creatine distribution in idiopathic inflammatory myopathy (IIM) patients' myocardial segments and investigate whether cardiovascular magnetic resonance (CMR) chemical exchange saturation transfer (CEST) creatine mapping can detect subclinical myocardial changes, CEST's ability was further compared with other conventional CMR mapping sequences. METHODS: Forty IIM patients (53.5 ± 10.5 years, 26 males) and eight healthy controls (35.4 ± 6 years, 5 males) underwent CMR scans on a 3.0-T MR scanner. Patients with IIM were further classified into two subgroups according to cardiac troponin T (cTn-T) values: the elevated cTn-T subgroup (n = 14) and the normal cTn-T subgroup (n = 26). Cine imaging, T2 SPAIR, LGE imaging, T1 mapping, T2 mapping, and Cr (creatine) CEST were performed. RESULTS: Cr mapping showed significantly reduced creatine in IIM patients among global myocardium (IIM: 0.109 ± 0.063, controls: 0.121 ± 0.021, p < 0.05), and decreased creatine signals were detected in all 16 cardiac segments (p < 0.05). Patients also had significantly prolonged native T1 and decreased enhanced T1 values in each cardiac segment (p < 0.05). There was no significant difference of LVEF and T2 values between IIM patients and controls. Between the two subgroups, elevated cTn-T was linked with creatine and extracellular volume fraction (ECV) values, providing a global average creatine signal of 0.107 vs 0.112 (p < 0.05) and 24.7 vs 32.4 (p < 0.05). CONCLUSION: Creatine CEST mapping can detect early-stage heart involvement with negative LGE findings in IIM. Compared with T1 mapping, CEST provides increased sensitivity to ECV measurement, making it significantly better than T1, and a promising CMR sequence for screening subclinical myocardial damage. KEY POINTS: • IIM patients with potential or ongoing heart involvement, elevated ECV, and reduced Cr CEST values could provide valuable information. • ECV and Cr CEST values were closely related to elevated cTn-T.

18.
Ann Hematol ; 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36622392

RESUMO

Acquired aplastic anemia (AA) is a bone marrow failure disorder characterized by pancytopenia, and immunosuppressive therapy (IST) is the optional first-line management. Several studies identified the influencing factors on IST response; however, there are still a considerable number of patients suffering from poor prognoses. In this study, we enrolled 61 AA patients aged ≤ 40 years old, and whole-exome sequencing (WES) found unexpected high FANC heterozygous germline mutations (28/61, 45.9%). Patients with FANC mutations have a significantly lower absolute reticulocyte count and CD34+ % in the bone marrow and also lower 3-, 6-, and 9-month IST response than that without mutation, which were 0% vs. 25% (P = 0.017), 26.3% vs. 42.1% (P = 0.495), and 29.4% vs. 72.2% (P = 0.011), especially in anti-thymocyte globulin combined with the cyclosporin A (ATG + CsA) group, which were 0% vs.33.4% (P = 0.143), 25% vs.83.3% (P = 0.103), and 25% vs. 100% (P = 0.003), respectively. The event-free survival in the FANCwt group was also better than that in the FANCmut group (P = 0.016) and also showed in patients who received ATG + CsA treatment (P = 0.045). In addition, all the adverse effects of FANC germline mutation were not significant in stem cell-transplanted group. Our result indicated that the WES-based detection of FANC heterozygous germline mutations may have a great meaning in predicting IST response of acquired AA. This study was registered at chictr.org.cn (# ChiCTR2100054992).

19.
Artigo em Inglês | MEDLINE | ID: mdl-36622608

RESUMO

Understanding long-term dynamics is vitally important for explaining current biodiversity patterns and setting conservation goals in a changing world. However, the changes in biodiversity in time and space, particularly the dynamics at the centuries or even longer time scales, are poorly documented because of a lack of continuous monitoring data. The sedimentary DNA (sedDNA) has a great potential for paleo-community reconstruction, and it has recently been used as a powerful tool to characterize past dynamics in terms of biodiversity over geological timescales. In particular, it is useful for prokaryotes and eukaryotes that do not fossilize; hence, it is revolutionizing the scope of paleoecological research. Here, a "Research Weaving" method was performed with systematic maps and bibliometric webs based on the Web of Science for Science Citation Index Expanded, presenting a comprehensive landscape of the sedDNA that traces biological dynamics. We identified that most sedDNA-based studies have focused on microbial dynamics and on using samples from multitypes of sediments. This review summarized the advantages and common applications of sedDNA, focused on the biodiversity in microbial communities, and provided an outlook for the future of sedDNA research.

20.
BMC Musculoskelet Disord ; 24(1): 38, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36650475

RESUMO

OBJECTIVE: The purpose of this study was to investigate the efficacy of Kinesio taping (KT) combined with multi-angle isometric resistance training for cervical spondylosis. METHODS: Sixty-one patients were divided into two groups by random number table method. Both groups were given multi-angle isometric training, the patients in the observation group were supplemented with Kinesio taping. Before and after treatment, the symptoms of cervical spine function were evaluated in two groups by visual analogue scale (VAS), cervical dysfunction index (NDI), cervical range of motion and muscle stiffness. RESULTS: After 3 weeks of treatment, VAS, NDI scores and the cervical range of motion were significantly better than before (P < 0.05). The range of anterior flexion and extension was significantly larger than the control group (P < 0.05), but the range of other motions were not certain. The muscle stiffness in KT group were significantly lower than the control group. CONCLUSION: Kinesio taping combined with multi-angle isometric resistance training can further alleviate the clinical symptoms and correct the neck abnormal posture. But its effects on the range of cervical motion remain uncertain.


Assuntos
Fita Atlética , Espondilose , Humanos , Pescoço , Vértebras Cervicais , Cervicalgia/terapia , Músculos , Amplitude de Movimento Articular/fisiologia , Espondilose/terapia
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