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1.
Talanta ; 221: 121493, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076098

RESUMO

The non-specific adsorption of protein has caused many problems in the application of materials. In this paper, a tri-block copolymer PEO-PNIPAAm-PSPMAP with double effects were obtained via atom transfer radical copolymerization (ATRP). The double-effect copolymer is covalently bonded to the hydrophobic material through a photosensitizer to achieve surface modification and applied to analytical chemistry. Sufficient hydratable groups (for instance, ether bonds, amide groups, and sulfonic acid groups) in the copolymer provides a basis for the anti-protein adsorption. At the same time, the interaction of the hydrophilic group and isopropyl group with temperature changes provides the possibility of elastic self-cleaning of the material, which is instrumental in extending the circulate lifetime of materials. Therefore, it is an environmentally friendly coating material. Besides, the effective antifouling performance and elastic self-cleaning function of the coating have been confirmed by the dynamic adsorption experiment of a fluorescent protein. The coating is used in capillary electrophoresis (CE), and its excellent protein separation spectrum verifies the practicality of the coating.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33048675

RESUMO

Local feature descriptor learning aims to represent distinctive images or patches with the same local features, where their representation is invariant under different types of deformation. Recent studies have demonstrated that descriptor learning based on Convolutional Neural Network (CNN) is able to improve the matching performance significantly. However, they tend to ignore the importance of sample selection during the training process, leading to unstable quality of descriptors and learning efficiency. In this paper, a dual hard batch construction method is proposed to sample the hard matching and non-matching examples for training, improving the performance of the descriptor learning on different tasks. To construct the dual hard training batches, the matching examples with the minimum similarity are selected as the hard positive pairs. For each positive pair, the most similar non-matching example is then sampled from the generated hard positive pairs in the same batch as the corresponding negative. By sampling the hard positive pairs and the corresponding hard negatives, the hard batches are produced to force the CNN model to learn the descriptors with more efforts. In addition, based on the above dual hard batch construction, an ℓ22 triplet loss function is built for optimizing the training model. Specifically, we analyze the superiority of the ℓ22 loss function when dealing with hard examples, and also demonstrate it in the experiments. With the benefits of the proposed sampling strategy and the ℓ22 triplet loss function, our method achieves better performance compared to state-of-the-art on the reference benchmarks for different matching tasks.

4.
Drug Deliv ; 27(1): 1474-1490, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33100061

RESUMO

The emergence of nanomaterials for drug delivery provides the opportunity to avoid the side effects of systemic drug administration and injury caused by the removal of tumors, delivering great promise for future cancer treatments. However, the efficacy of current nano drugs is not significantly better than that of the original drug treatments. The important reason is that nano drugs enter the tumor vasculature, remaining close to the blood vessels and unable to enter the tumor tissue or tumor cells to complete the drug delivery process. The low efficiency of drug penetration into tumors has become a bottleneck restricting the development of nano-drugs. Herein, we present a systematic overview of recent advances on the design of nano-drug carriers in drug delivery systems for enhancing drug penetration into tumors. The review is organized into four sections: The drug penetration process in tumor tissue includes paracellular and transcellular transport, which is summarized first. Strategies that promote tumor penetration are then introduced, including methods of remodeling the tumor microenvironment, charge inversion, dimensional change, and surface modification of ligands which promote tissue penetration. Conclusion and the prospects for the future development of drug penetration are finally briefly illustrated. The review is intended to provide thoughts for effective treatment of cancer by summarizing strategies for promoting the endocytosis of nano drugs into tumor cells.

5.
Biomater Sci ; 2020 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-33107517

RESUMO

In recent years, liposomes have been used in the field of biomedicine and have achieved many significant results. Because of the unique characteristics of liposomes, there are many materials that can be loaded in many types of liposome, such as biologically active substances. Liposomes are made of lipid bilayers, which are very similar to cell membranes. They have many biological and technical advantages and have even been used in clinical practice because of their unique structure. As liposomes have hydrophobic cavities and hydrophilic cavities, they can simultaneously load hydrophobic and hydrophilic drugs. This causes an increase in the drug loading efficiency and therapeutic capability. Different therapeutic effects are achieved by different methods such as placing the biological materials in the bilayer structure of the liposome or encapsulating them in the liposome. By these methods, bio-simulated liposomes can achieve considerable cancer treatment effects. Now liposomes have become an independent platform and play an important role in many facets. In this review, we focus on the use of liposomes composed of biomaterials for the treatment of cancer. These include liposomes that mimic mammalian cells, bacteria, and viruses.

6.
Sci Total Environ ; 755(Pt 2): 142530, 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-33039931

RESUMO

As the largest contributor to production-based emissions, electricity generation sector has led to huge carbon emission transmissions. This is the first attempt to explore the latest features of carbon emission transmissions from electricity sector to the final domestic consumption of China in 2002-2015, combining MRIO-based Structural Path Analysis and transmission-based emission method. Results show that: (1) Although inter-provincial transmissions are increasing significantly, emission transmissions within intra-provincial trading are dominated. (2) 30 provinces are classified into two types, i.e., consumption centers and production centers. Both the inter-provincial transmission paths in consumption centers and production centers show the grid-level agglomeration and provincial heterogeneity. The inflow paths in consumption centers are mainly sourced from the production of Eastern China and South China, while the outflow paths for production centers are caused by the consumption in Central China, Guangdong and Jiangsu. Inter-provincial linkages are intensified and perform the feature of territorial propinquity. (3) Both intra-grid and inter-grid transmission nodes show an agglomeration trend of "electricity sector < intermediate sectors < electricity sector < consumption". These intermediate sectors include manufacture sectors, energy-intensive sectors and service sector. This paper provides policy implications on promoting low-carbon electricity cooperation across provinces and managing intermediate transmissions along supply chain.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33011842

RESUMO

As a common mental health problem, social anxiety refers to the fear and avoidance of interacting in social or performance situations, which plays a crucial role in many health and social problems. Although a growing body of studies has explored the neuroanatomical alterations related to social anxiety in clinical patients, far fewer have examined the association between social anxiety and brain morphology in the general population, which may help us understand the neural underpinnings of social anxiety more comprehensively. Here, utilizing a voxel-based morphometry approach via structural magnetic resonance imaging, we investigated brain gray matter correlates of social anxiety in 231 recent graduates of the same high school grade. We found that social anxiety was positively associated with gray matter volume in the right middle temporal gyrus (MTG), which is a core brain area for cognitive processing of emotions and feelings. Critically, emotional intelligence mediated the impact of right MTG volume on social anxiety. Notably, our results persisted even when controlling for the effects of general anxiety and depression. Altogether, our research reveals right MTG gray matter volume as a neurostructural correlate of social anxiety in a general sample of adolescents and suggests a potential indirect effect of emotional intelligence on the association between gray matter volume and social anxiety.

8.
Ann Plast Surg ; 85(4): 430-436, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32931683

RESUMO

PURPOSE: This study aimed to investigate the repair of bone defects in rabbits with tissue-engineered bones using cocultured endothelial progenitor cells (EPCs) and bone marrow mesenchymal stem cells (BMSCs) as seeding cells. METHODS: Endothelial progenitor cells and BMSCs were isolated and purified from the peripheral blood and bone marrow, respectively, of New Zealand rabbits. The third passage of BMSCs was cultured alone or with EPCs. Cells were characterized using specific markers and then seeded on partially deproteinized biologic bones from pigs as a scaffold. The engineered bones were used to repair bone defects in rabbits. Hematoxylin and eosin and Masson staining were performed to examine vascularization and osteogenesis in the engineered bone. RESULTS: The cocultured EPCs and BMSCs grew well on the surface of the scaffold. Compared with monocultured BMSCs, cocultured EPCs and BMSCs promoted the formation of blood vessels and bone on the scaffold, in addition to accelerating the repair of bone defects. The collagen content was significantly increased in the scaffold with cocultured EPCs and BMSCs, compared with the scaffold seeded with mono-cultured BMSCs. CONCLUSIONS: Tissue-engineered bones seeded with cocultured EPCs and BMSCs may be used effectively for the repair of bone defects.

9.
Adv Healthc Mater ; : e2000429, 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32940022

RESUMO

Dynamic measurements of steroid hormones in vivo are critical, but steroid sensing is currently limited by the availability of specific molecular recognition elements due to the chemical similarity of these hormones. In this work, a new, self-templating synthetic approach is applied using corona phase molecular recognition (CoPhMoRe) targeting the steroid family of molecules to produce near infrared fluorescent, implantable sensors. A key limitation of CoPhMoRe has been its reliance on library generation for sensor screening. This problem is addressed with a self-templating strategy of polymer design, using the examples of progesterone and cortisol sensing based on a styrene and acrylic acid copolymer library augmented with an acrylated steroid. The pendant steroid attached to the corona backbone is shown to self-template the phase, providing a unique CoPhMoRE design strategy with high efficacy. The resulting sensors exhibit excellent stability and reversibility upon repeated analyte cycling. It is shown that molecular recognition using such constructs is viable even in vivo after sensor implantation into a murine model by employing a poly (ethylene glycol) diacrylate (PEGDA) hydrogel and porous cellulose interface to limit nonspecific absorption. The results demonstrate that CoPhMoRe templating is sufficiently robust to enable a new class of continuous, in vivo biosensors.

10.
Sci Rep ; 10(1): 14354, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32873822

RESUMO

This paper describes a minimally invasive technique of percutaneous intervertebral bridging cementoplasty (PIBC) to augment the fractured vertebrae and immobilize the intervertebral space with endplate-disc complex injury simultaneously. Thirty-two patients with adjacent multilevel osteoporotic thoracolumbar fractures (AMOTLFs) and vertebral endplate-disc complex injury (EDCI) treated by PIBC were retrospectively reviewed. The PIBC technique was a combination of puncture, balloon expansion and bridging cementoplasty. The clinical and radiological assessments were reviewed. The operation time was 82.8 ± 32.5 min, and blood loss was 76.9 ± 31.7 mL. A cement bridge was connected between the two fractured vertebrae across the injured intervertebral space. VAS at three time points including pre-operation, post-operation 1 day and final follow-up was 6.9 ± 0.9, 2.9 ± 0.8 and 1.7 ± 0.8, respectively; ODI at three time points was (71.1 ± 7.8)%, (18.4 ± 5.7)%, and (10.3 ± 5.7)%, respectively; Cobb angle at three time points was 46.0° ± 10.4°, 25.9° ± 8.5°, and 27.5° ± 7.1°, respectively. Compared with pre-operation, VAS, ODI and Cobb angle were significantly improved at post-operation 1 day and final follow-up (P < 0.05). Clinical asymptomatic cement leakage was observed in thirteen patients. No vessel or neurological injury was observed. PIBC may be an alternative way of treatment for AMOTLFs with EDCI. The technique is a minimally invasive surgery to augment the fractured vertebrae and immobilize the injured intervertebral space simultaneously.

11.
J Psychiatr Res ; 131: 60-68, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32937251

RESUMO

BACKGROUND: The human brain activity is inherently dynamic over time. Conventional neuroimaging studies have reported abnormalities of static intrinsic brain activity or connectivity in adolescent patients with conduct disorder (CD). Little is known, however, regarding the temporal dynamics alterations of brain activity in CD. METHODS: In this study, resting-state functional magnetic resonance imaging examinations were performed on adolescent patients with pure CD and age-matched typically developing (TD) controls. The dynamic amplitude of low-frequency fluctuation (dALFF) was first measured using a sliding-window method. The temporal variability (TV) was then quantified as the variance of dALFF over time and compared between the two groups. Further, the relationships between aberrant TV of dALFF and clinical features were evaluated. RESULTS: CD patients showed reduced brain dynamics (less temporal variability) in the default-mode network, frontal-limbic cortices, sensorimotor areas, and visual regions which are involved in cognitive, emotional and perceptional processes. Importantly, receiver operating characteristic curve analysis revealed that regions with altered TV of dALFF exhibited a better ability to distinguish CD patients than the results from static ALFF in the current data set. CONCLUSIONS: Our findings extended previous work by providing a novel perspective on the neural mechanisms underlying adolescent patients with CD and demonstrated that the altered dynamic local brain activity may be a potential biomarker for CD diagnosis.

12.
Adv Colloid Interface Sci ; 284: 102254, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32942182

RESUMO

Protein, as the material basis of vita, is the crucial undertaker of life activities, which constitutes the framework and main substance of human tissues and organs, and takes part in various forms of life activities in organisms. Separating proteins from biomaterials and studying their structures and functions are of great significance for understanding the law of life activities and clarifying the essence of life phenomena. Therefore, scientists have proposed the new concept of proteomics, in which protein separation technology plays a momentous role. It has been diffusely used in the food industry, agricultural biological research, drug development, disease mechanism, plant stress mechanism, and marine environment research. In this paper, combined with the recent research situation, the progress of protein separation technology was reviewed from the aspects of extraction, precipitation, membrane separation, chromatography, electrophoresis, molecular imprinting, microfluidic chip and so on.

13.
Nat Commun ; 11(1): 4664, 2020 09 16.
Artigo em Inglês | MEDLINE | ID: mdl-32938919

RESUMO

Cardiorenal syndrome type 4 (CRS4) is a common complication of chronic kidney disease (CKD), but the pathogenic mechanisms remain elusive. Here we report that morphological and functional changes in myocardial mitochondria are observed in CKD mice, especially decreases in oxidative phosphorylation and fatty acid metabolism. High phosphate (HP), a hallmark of CKD, contributes to myocardial energy metabolism dysfunction by downregulating peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α). Furthermore, the transcriptional factor interferon regulatory factor 1 (IRF1) is revealed as the key molecule upregulated by HP through histone H3K9 acetylation, and responsible for the HP-mediated transcriptional inhibition of PGC1α by directly binding to its promoter region. Conversely, restoration of PGC1α expression or genetic knockdown of IRF1 significantly attenuates HP-induced alterations in vitro and in vivo. These findings demonstrate that IRF1-PGC1α axis-mediated myocardial energy metabolism remodeling plays a crucial role in the pathogenesis of CRS4.


Assuntos
Síndrome Cardiorrenal/metabolismo , Fator Regulador 1 de Interferon/metabolismo , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Síndrome Cardiorrenal/patologia , Modelos Animais de Doenças , Regulação para Baixo , Metabolismo Energético , Técnicas de Silenciamento de Genes , Taxa de Filtração Glomerular , Glucuronidase/genética , Insuficiência Cardíaca/etiologia , Insuficiência Cardíaca/metabolismo , Humanos , Fator Regulador 1 de Interferon/genética , Masculino , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Pessoa de Meia-Idade , Mitocôndrias Cardíacas/metabolismo , Mitocôndrias Cardíacas/patologia , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/genética , Fosfatos/metabolismo , Regiões Promotoras Genéticas , Ratos , Insuficiência Renal Crônica/complicações , Insuficiência Renal Crônica/metabolismo , Adulto Jovem
14.
Viruses ; 12(9)2020 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-32887282

RESUMO

Bovine herpesvirus 1 (BoHV-1) is an alphaherpesvirus that causes great economic losses in the cattle industry. Herpesvirus infection generally induces endoplasmic reticulum (ER) stress, and the unfolded protein response (UPR) in infected cells. However, it is not clear whether ER stress and UPR can be induced by BoHV-1 infection. Here, we found that ER stress induced by BoHV-1 infection could activate all three UPR sensors (the activating transcription factor 6 (ATF6), the inositol-requiring enzyme 1 (IRE1), and the protein kinase RNA-like ER kinase (PERK)) in MDBK cells. During BoHV-1 infection, the ATF6 pathway of UPR did not affect viral replication. However, both knockdown and specific chemical inhibition of PERK attenuated the BoHV-1 proliferation, and chemical inhibition of PERK significantly reduced the viral replication at the post-entry step of the BoHV-1 life cycle. Furthermore, knockdown of IRE1 inhibits BoHV-1 replication, indicating that the IRE1 pathway may promote viral replication. Further study revealed that BoHV-1 replication was enhanced by IRE1 RNase activity inhibition at the stage of virus post-entry in MDBK cells. Furthermore, IRE1 kinase activity inhibition and RNase activity enhancement decrease BoHV1 replication via affecting the virus post-entry step. Our study revealed that BoHV-1 infection activated all three UPR signaling pathways in MDBK cells, and BoHV-1-induced PERK and IRE1 pathways may promote viral replication. This study provides a new perspective for the interactions of BoHV-1 and UPR, which is helpful to further elucidate the mechanism of BoHV-1 pathogenesis.

15.
Eur J Surg Oncol ; 46(10 Pt B): e55-e61, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32782201

RESUMO

BACKGROUND: The aim of this study was to evaluate the safety of natural orifice specimen extraction surgery (NOSES) and to compare the short- and long-term outcomes of three techniques of NOSES for rectal cancer (RC). MATERIALS AND METHODS: A consecutive series of RC patients in stage I-III who underwent laparoscopic NOSES were enrolled. Three main techniques of NOSES included specimen eversion and extra-abdominal resection (EVER), specimen extraction and extra-abdominal resection (EXER) and intra-abdominal resection and specimen extraction (IREX). The postoperative complications, 5-year disease free survival (DFS), 5-year local recurrence rate (LRR) and 5-year distant metastasis rate (DMR) were compared in three techniques. RESULTS: 268 RC patients met inclusion criteria, including 83 patients treated with EVER, 75 patients treated with EXER and 110 patients treated with IREX. Tumor location was the most critical factor associated with technique selection, with P < 0.001. Postoperative complication rate was 12.3% for all patients, and it was 18.1% for EVER, 13.3% for EXER and 7.3% for IREX. There were no significant differences for anastomotic leakage, anastomotic bleeding and intraabdominal abscess among three technique groups, with P > 0.05. For long-term outcomes, the 5-year DFS, 5-year LRR and 5-year DMR were 85.03%, 4.22% and 11.00% for all patients. Patients in advanced tumor stage have worse long-term survival compared with patients in early stage, but no significant survival differences were observed among three technique groups. CONCLUSION: Three techniques of NOSES for RC had acceptable short- and long-term outcomes, and tumor location was a determinant of technique selection.

16.
EBioMedicine ; 58: 102910, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32739867

RESUMO

BACKGROUND: Abnormalities of functional activation and cortical volume in brain regions involved in the neurobiology of fear and anxiety have been implicated in the pathophysiology of social anxiety disorder (SAD). However, few studies have performed separate measurements of cortical thickness (CT) and cortical surface area (CSA) which reflect different neurobiological processes. Thus, we aimed to explore the cortical morphological anomaly separately in SAD using FreeSurfer. METHODS: High-resolution structural magnetic resonance images were obtained from 32 non-comorbid never-treated adult SAD patients and 32 demography-matched healthy controls. Cortical morphometry indices including CT and CSA were separately determined by FreeSurfer and compared between the two groups via whole-brain vertex-wise analysis, while partial correlation analysis using age and gender as covariates were conducted. FINDINGS: The patients with SAD showed decreased CT but increased CSA near-symmetrically in the bilateral prefrontal cortex (PFC) of the dorsolateral, dorsomedial, and ventromedial subdivisions, as well as the right lateral orbitofrontal cortex; increased CSA in the left superior temporal gyrus (STG) was also observed in SAD. The CSA in the left PFC was negatively correlated with the disease duration. INTERPRETATION: As the balloon model hypothesis suggests that the tangentially stretched cortex may cause dissociations in cortical morphometry and affect the cortical capacity for information processing, our findings of dissociated morphological alterations in the PFC and cortical expansion in the STG may reflect the morphological alterations of the functional reorganization in those regions, and highlight the important role of those structures in the pathophysiology and neurobiology of SAD. FUNDING: This study was funded by the National Natural Science Foundation of China (Grant Nos. 31700964, 31800963, 81621003, and 81820108018).

17.
Pathol Res Pract ; 216(9): 153101, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32825966

RESUMO

Synaptotagmin 7 (SYT7) can encode a single-pass 46-kDa transmembrane protein which located on human chromosome 11q12.2. It has been reported to be dysregulated in several cancers; however, there are few reports on the role of SYT7 in non-small cell lung carcinoma (NSCLC). The purpose of our study was to investigate the expression of SYT7 in NSCLC and its relationship with the prognosis of NSCLC. Differences in SYT7 expression were explored by using a public database and tissue samples. The prognostic value of SYT7 and its expression correlation with clinical parameters were evaluated by statistical analysis. Our current study found that elevated mRNA and protein levels of SYT7 in NSCLC tissues compared to adjacent normal tissues. The high expression of SYT7 in NSCLC patients was positively correlated with tumour differentiation (P = 0.031) and pT (P = 0.041). The higher SYT7 expression had a shorter survival time than those with lower SYT7 expression in NSCLC patients. Furthermore, multivariate analysis demonstrated that the expression of SYT7 was an unfavourable independent prognostic factor for NSCLC (P = 0.044). In conclusion, SYT7 was upregulated in NSCLC tissues and maybe a prognostic and diagnostic factor of NSCLC.

18.
Curr Med Sci ; 40(4): 642-645, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32767262

RESUMO

The SARS-CoV-2 infection status of hospitalized children was surveyed in the department of pediatric hematology and oncology in three different hospitals of epidemic areas in Hubei, China. A cross-sectional study was performed to investigate the clinical characteristics, lung CT scan, SARS-CoV-2 nucleic acid test and serum antibodies of hospitalized children with hemato-oncological diseases from January 23 to April 24, 2020. 299 children were enrolled in this study, including 176 males (58.9%) and 123 females (41.1%), aged from 2 months to 16 years. 255 cases (85.3%) received chemotherapy or other immunosuppressive therapies, and there were 44 cases (14.7%) of other benign diseases. Nucleic acid test was performed on 258 children (86.3%) and one case was positive. 163 cases (54.5%) were tested for serum antibodies, and all of them were negative. Lung CT scan was performed on 247 children (82.6%), and 107 of them showed infectious changes. Only one case (0.33%) of COVID-19 was diagnosed in the group. The prevalence rate of COVID-19 in enrolled children with hemato-oncological diseases in Hubei was 0.33%. Immunosuppressed patients are not prone to produce related antibodies. Comprehensive protective measures and ward management can reduce the risk of SARS-CoV-2 infection in the group patients.


Assuntos
Betacoronavirus , Infecções por Coronavirus/epidemiologia , Doenças Hematológicas/epidemiologia , Neoplasias/epidemiologia , Pandemias , Pneumonia Viral/epidemiologia , Adolescente , Criança , Criança Hospitalizada , Pré-Escolar , China/epidemiologia , Técnicas de Laboratório Clínico/métodos , Comorbidade , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/prevenção & controle , Estudos Transversais , Feminino , Humanos , Lactente , Controle de Infecções , Masculino , Programas de Rastreamento , Pandemias/prevenção & controle , Pneumonia Viral/diagnóstico , Pneumonia Viral/prevenção & controle , Prevalência , Fatores de Risco , Inquéritos e Questionários
19.
PeerJ ; 8: e9565, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32765968

RESUMO

This study describes two new species of freshwater crab of the genus Heterochelamon Türkay & Dai, 1997 from southern China, H. huidongense from Guangdong Province and H. jinxiuense from Guangxi Zhuang Autonomous Region. The two new species can be differentiated from congeners by characters derived from the shape of the epibranchial tooth, external orbital angle, cheliped proportions and structure of the male first gonopod. The present study brings the number of Heterochelamon species to seven. We used the mitochondrial 16S rRNA gene for a molecular analysis and the results are consistent with the morphological features that support the recognition of two new taxa.

20.
Am J Gastroenterol ; 2020 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-32769422

RESUMO

INTRODUCTION: Celiac disease (CeD) is a lifelong immune-mediated enteropathy in which dietary gluten triggers an inflammatory reaction in the small intestine. This retrospective cohort study examines healthcare resource utilization (HRU) and costs between patients with CeD and matched controls. METHODS: Patients with CeD (cases) with an endoscopic biopsy and ≥2 medical encounters with a CeD diagnosis between January 1, 2010, and October 1, 2015, were identified in the MarketScan databases. The date of the first claim with a CeD diagnosis on or after the endoscopic biopsy was the index date. Cases were matched 1:1 to patients without CeD (controls) on demographic characteristics and Deyo-Charlson Comorbidity Index score. Clinical characteristics, all-cause, and CeD-related HRU and costs (adjusted to 2017 US dollars) were compared between cases and controls during the 12 months before (baseline) and 24 months after (follow-up) the index date. RESULTS: A total of 11,008 cases (mean age 40.6 years, 71.3% women) were matched to 11,008 controls. During the follow-up, a higher proportion of cases had all-cause and CeD-related HRU including inpatient admissions, emergency department visits, gastroenterologist visits, dietician visits, endoscopic biopsies, and gastroenterology imaging (all P ≤ 0.002). Incremental all-cause and CeD-related costs were in the first ($7,921 and $2,894) and second ($3,777 and $935) year of follow-up, driven by outpatient services costs. DISCUSSION: In this US national claims database analysis, there was evidence of an increase in both all-cause and CeD-related HRU and related costs in patients with CeD compared with matched patients without CeD, suggesting a significant economic burden associated with CeD.

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