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1.
J Nanosci Nanotechnol ; 20(4): 2543-2549, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492274

RESUMO

In this work, crystalline Si nanoparticles are synthesized on a large scale via a low temperature molten salt method. The crystal morphologies and electrochemical properties of the samples after HCl and HF leaching are studied in detail. The electrochemical properties of the as-produced silicon samples, which are used as anode materials in lithium-ion batteries (LIBs), are evaluated. The final product, which contains more Si and less SiO², improves cyclic stability because it buffers the volumetric effect of Si during lithiation/delithiation. The HF acid leach removes the impurity of SiO². However, the prepared sample exhibits lower electrochemical properties. The sample with a low SiO² content can deliver a capacity of 1503 mAh g-1 after 50 cycles at a higher current density of 1 A g-1, and the Coulombic efficiency is approximately 100%. However, the sample after HF acid leaching only delivers a discharge capacity of 389 mAh g-1 at 1 A g-1 after 50 cycles.

2.
Int J Med Mushrooms ; 21(7): 683-691, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31679302

RESUMO

A skin substitute TG05 obtained from residues of the culinary-medicinal mushroom Flammulina velutipes cultivation process was developed in this study for the first time. Pre-column derivatization high-performance liquid chromatography fingerprints analysis revealed that TG05 was composed of water-insoluble fibers containing xylose (57%), glucose (19.5%), and arabinose (16.3%) as major monomers. Porous and opaque structure of TG05 was demonstrated by scanning electron microscopy. Animal experiments conducted on mice and rats indicated that TG05 notably accelerated the wound-healing process. In addition, TG05 induced proliferation and migration of human keratinocytes time- and dose-dependently. Taken together, the skin substitute TG05 with new structure promotes wound healing in vitro and in vivo. This study provided a novel method to produce functional biomaterial from abundant and low-cost agricultural residues generated during edible mushroom cultivation.

3.
Mini Rev Med Chem ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686638

RESUMO

Cytisine is a quinolizidine alkaloid isolated from various Leguminosae plants. Cytisine and its derivatives exhibit a broad range of biological properties, such as smoking cessation aid, antidepressant, neuroprotective, nootropic, anticancer, antiviral, antiparasitic, antidiabetic, insecticidal, and nematicidal activities. In this review, the progress of cytisine and its derivatives in regard to bioactivities, total synthesis, structural modifications focusing on their N-12 position and lactam ring is reported. Additionally, structure-activity relationships of cytisine and its derivatives are also discussed.

4.
Chem Commun (Camb) ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31687693

RESUMO

In this study, ultrathin α-Co(OH)2 nanosheets intercalated with molybdate were successfully synthesized using a one-step coprecipitation method, and the derived Co-Mo-O mixed metal oxides demonstrated outstanding catalytic performance in the aerobic oxidative desulfurization (AODS) of fuels using molecular oxygen (O2) in air as the oxidizing agent.

5.
Medicine (Baltimore) ; 98(44): e17738, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689821

RESUMO

This study aimed to evaluate a novel real-time rotational optical coherence tomography (OCT) imaging system (OCTIS) with a fiber-optic probe to look at endometrium and to correlate the OCTIS images with standard histology. OCT could obtain real-time images resembling histological examination. With recent development of customized probes, it allows OCT to be used in the field of gynecology.This is a pilot, prospective, ex-vivo and observational study. Women underwent hysterectomy for various gynecological conditions were recruited and OCTIS images were obtained from endometrium of 15 fresh uterus specimens immediately after hysterectomy. The excised uterus was cut open and OCTIS imaging was obtained. The scanned region of endometrium was excised for histological examination and OCTIS images were precisely compared to corresponding histological images and ultrasound images. Blinded qualitative analysis on OCTIS images was performed by 2 assessors to determine inter-rating reliability on the histopathological diagnosis.Epithelium, glands, cysts, and stroma of endometrium were clearly seen by the OCTIS. Different phases of menstrual cycle of normal endometrium could be differentiated and pathological condition such as hyperplastic and dysplasic endometrium, which corresponded well with histological findings, could be identified. The inter-rater reliability between assessors on overall OCTIS endometrium and neoplastic OCTIS endometrium was moderate (Kendall τb of 0.58) and substantial (Kendall τb of 0.76), respectively.OCTIS can provide real-time, high-resolution and rotational imaging modality to view endometrial structure with high consistency with histological examination and satisfactory agreement between observers. It has a great potential to be developed in the clinical use of endometrial assessment for gynecological applications.

6.
J Am Acad Dermatol ; 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31669080

RESUMO

BACKGROUND: Moderate-to-severe atopic dermatitis/AD is increasingly recognized as a systemic disease, largely due to proteomic blood studies. There are growing efforts to develop AD biomarkers utilizing minimal tissues. OBJECTIVE: To characterize the AD skin proteomic signature and its relationship with the blood proteome, and genomic skin profile in the same individuals. METHODS: We evaluated lesional and non-lesional biopsies and blood from moderate-to-severe AD (n=20) and healthy (n=28) individuals using OLINK proteomics (utilizing 10µg/10µL for skin and blood) and RNAseq (skin). RESULTS: The AD skin proteome demonstrated significant upregulation in lesional and even in non-lesional skin compared to controls in inflammatory markers (MMP12; Th2/IL1RL1/IL-33R, IL-13, CCL17; Th1/ CXCL10; Th17/Th22/PI3, CCL20, S100A12), and in cardiovascular-associated proteins (SELE, MMPs, PGF, MPO, FABP4, VEGFA; FDR<0.05). Skin proteins demonstrated much higher and significant upregulations (vs. controls) compared to blood, suggesting a skin source for the inflammatory/cardiovascular profile. Gene and protein expressions were correlated (r=0.410, P<0.001), with commonly upregulated inflammatory and cardiovascular-risk products, suggesting protein translation in skin. LIMITATIONS: Our analysis was limited to 354 proteins. CONCLUSIONS: The AD skin proteome shows an inflammatory and cardiovascular signature even in non-lesional skin, emphasizing the need for proactive treatment. Skin proteomics presents a sensitive option for biomarker monitoring.

7.
Med Sci Monit ; 25: 8172-8180, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670317

RESUMO

BACKGROUND Baicalin, one of the main bioactive components extracted from the traditional Chinese medicine baical Skullcap root, has an anti-tumor activity which had been studied in several cancers. However, its role in human mesothelioma remains unknown. In this study, we investigated the anti-tumor mechanisms of baicalin in the mesothelioma cell line MESO924. MATERIAL AND METHODS Effects of baicalin on mesothelioma were assessed by measuring cell viability, apoptosis, migration, invasion, inactivation of signaling intermediates, and cell-cycle alterations. RESULTS Baicalin inhibited the proliferation, migration, and invasion of human mesothelioma cells and increased their apoptosis, all in a dose-dependent manner. Specifically, baicalin decreased the expression of p-EGFR, p-AKT, p-MAPK, p-S6, Bcl-2, and VEGF and increased the expression of Bax in mesothelioma cells. The suppressed mesothelioma cellular proliferation is due to the arrest of the S cell cycle by baicalin. Inhibition of the PI3K/AKT/mTOR signaling pathway by a PI3K/AKT/mTOR inhibitor augmented the anti-proliferation effects induced by baicalin. In addition, baicalin increased the sensitivity of MESO924 to the chemotherapeutic drugs doxorubicin, cisplatin, and pemetrexed. CONCLUSIONS These results highlight the roles of baicalin in inhibiting cell growth, migration, and invasion of mesothelioma cells while increasing apoptosis and sensitizing cells to chemotherapeutic agents through the PI3K/AKT/mTOR signaling pathway, which indicates that baicalin could be a useful drug for mesothelioma therapy.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31679783

RESUMO

Graphitic carbon nitride (g-C3N4) has aroused great concern since it applied to the photocatalytic process. However, the inherent shortcomings of bulk g-C3N4, such as small active surface area, low separation efficiency of photogenerated carriers, sluggish charge transport process, etc., result in low-level photocatalytic performance. The rich-nitrogen carbon nitride nanotubes (CNNTs) made by an easy supermolecule self-assembly method could slove these subsisting problems. The CNNTs with unique morphology possess superior separation/migration of photo-excited charge carriers and enhanced photocatalytic performance. Under irradiation with visible light (λ > 400 nm), measured with Pt (3 wt%) as co-catalyst, the CNNTs have a hydrogen evolution rate of 18.06 mmol h-1 g-1 and its apparent quantum yield (AQY) is 12.55% (420 nm). The CNNTs are also applied to degrade antibiotics with simultaneous hydrogen production, providing a method for alleviating energy crisis and environmental pollution issues. The degradation rate of bisphenol A (BPA) is 92% and simultaneously with 13.63 µmol photocatalytic hydrogen generation after irradiation for 5 h.

9.
Physiol Genomics ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31682178

RESUMO

Long non-coding RNAs have been implicated in the development and progression of atherosclerosis. However, the expression and mechanism of action of lncRNAs in atherosclerosis are still unclear. We implemented microarray analysis in human advanced atherosclerotic plaques and normal arterial intimae to detect the lncRNA and mRNA expression profile. Gene Ontology functional enrichment and pathway analyses were applied to explore the potential functions and pathways involved in the pathogenesis of atherosclerosis. A total of 236 lncRNAs and 488 mRNAs were selected for further Ingenuity Pathway Analysis. Moreover, qRT-PCR tests of most selected lncRNAs and mRNAs with high fold changes were consistent with the microarray data. We also performed ELISA to investigate the corresponding proteins levels of selected genes and showed that serum levels of SPP1, CD36, ATP6V0D2, CHI3L1, MYH11 and BDNF were differentially expressed in patients with coronary heart disease compared with healthy subjects.These proteins correlated with some biochemical parameters used in the diagnosis of cardiovascular diseases. Furthermore, receiver operating characteristic analysis showed a favorable diagnostic performance.The microarray profiling analysis and validation of differentially-expressed lncRNAs and mRNAs in atherosclerosis not only provide new insights into the pathogenesis of this disease, but may also reveal new biomarkers for its diagnosis and treatment.

10.
Math Biosci Eng ; 16(6): 6805-6821, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31698589

RESUMO

p62/SQSTM1 is the scaffold protein implicated in selective autophagy, which is induced by cellular stress. Research has shown that p62 is highly expressed in cancer. Moreover, p62 can easily promote tumor metastasis. However, studies have not reached a consensus on the relationship of p62 expression with the diagnosis and prognosis of lung cancer. We conducted a systematic review and meta-analysis of studies on p62 expression in the prognosis and clinical-pathological parameters of lung cancer patients. Literature search was performed with PubMed, Web of Science, EMBASE, Cochrane Library, and SpringerLink databases. Fixed-effects and random-effects models were used to study the relationship of p62 expression with patients' overall survival (OS) and clinical-pathological parameters. I2 was used to test for heterogeneity. Egger's test was used to assess publication bias. The meta-analysis collected and considered 13 articles, which included 1393 lung cancer patients. The studies show that the high expression of p62 is associated with poor OS in lung cancer patients. The clinical-pathological parameters of patients show that p62 is more highly expressed in high TNM stage (II + III + IV VS. I), Lymph node metastasis (N1 VS. N0), and distant metastases (D1 VS. D0). However, there is no correlation between the p62 expression and the Beclin 1 and LC3B in lung cancer patients. In conclusion, the over-expression of p62 is associated with poor OS in lung cancer patients and can be used as a biomarker for lung cancer diagnosis and prognosis.

11.
J Environ Manage ; 250: 109363, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31703242

RESUMO

The past two decades have witnessed growing global concern about excessive greenhouse gas (GHG) emissions by reservoirs and the development of hydropower. Literature review showed that life cycle GHG emissions per energy production of collected global dataset ranged from 0.04 to 237.0 gCO2eq/kW⋅h, with a mean of 25.8 ±â€¯3.0 gCO2eq/kW⋅h. Synthesis from the China's five largest hydro-projects and other publications estimated that the large- and mid-scale hydro-projects in China had a carbon footprint between 6.2 gCO2eq/kWh and 34.6 gCO2eq/kWh, with a mean value of 19.2 ±â€¯6.8 gCO2eq/kWh (mean ±â€¯sd.). Over 80% of the carbon footprint of the hydro-projects could be conservatively allocated to hydroelectricity generation, while the rest could then be allocated to flood control services. In the Three Gorges Dam Project, the allocated life cycle GHG emissions per energy production of its hydroelectricity production was estimated to be 17.8 gCO2eq/kW⋅h. GHG emissions from reservoir sediments and in the phase of operation and maintenance were still uncertain. There is still a need of in-depth research on reservoir carbon cycling to quantify net reservoir GHG emissions.

12.
J Hazard Mater ; : 121480, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31704118

RESUMO

Photoreduction of highly toxic Cr(VI) has been regarded as an efficient and green method to achieve water purification. In this process, better charge carrier separation is vital to achieving excellent performance. Besides, it is vital to systematically explore the influencing factors and reaction mechanism. Herein, a novel 3D PPy/Zn3In2S6 nanoflower composite was successfully fabricated via in-situ polymerization. The remarkable conductivity of PPy provides a good electron transport path to facilitate the separation and migration of charge carriers, which benefits to the activity improvement. The results show that 5% PPy/Zn3In2S6 exhibits superior photocatalytic activity with almost 100% Cr(VI) reduction just within 24 min and 99.4% of Methyl orange (MO) is degraded in 25 min. On this basis, factors of different catalyst dosage, concentration, ions and pH under the reduction system were systematically investigated. Especially, different organic acids were in-depth analyzed and the activity could be significantly enhanced just adding 0.1 mmol organic acids. 5% 3D PPy/Zn3In2S6 nanoflower composites (with tartaric acid) exhibits superior photocatalytic activity, which can achieve 100% photoreduction of Cr(VI) just within 6 min. Finally, a possible reaction mechanism was proposed. Moreover, 3D PPy/Zn3In2S6 nanoflower also presented an efficient photodegradation activity for organic pollution.

13.
J Clin Immunol ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31705453

RESUMO

PURPOSE: STING-associated vasculopathy with onset in infancy (SAVI) is a new rare auto-inflammatory disease. The purpose of this study is to report new cases and summarize the manifestations and outcome of SAVI. METHODS: We made a retrospective analysis of three pediatric patients diagnosed with SAVI between March 2016 and July 2018 in Beijing Children's Hospital. RESULTS: Three patients comprised one boy and two girls. The median age of onset was 4 months. All patients had the same de novo heterozygous mutation (c.463G>A, p. V155M) of TMEM173. All patients presented with interstitial lung disease and one coexisted with diffuse alveolar hemorrhage. Rashes were presented in two patients. Other clinical manifestations include febrile attacks, failure to thrive, arthritis, myositis, cerebrovascular involvement, ureteral calculus, gastroesophageal reflux, and malnutrition. Ground-glass opacities were the most common features of chest computed tomography, followed with cysts and reticular opacities. Transbronchial lung biopsy was performed in one patient revealing pulmonary vasculitis. Skin biopsy was performed in one patient with changes of vasculitis. All patients were treated with corticosteroids and two patients received combined treatment of tofacitinib. The therapeutic effects of tofacitinib were limited on interstitial lung disease in both patients and were poor on rashes in one patient. One patient under the treatment of tofacitinib died. CONCLUSIONS: New clinical aspect of diffuse alveolar hemorrhage is first reported to be associated with SAVI. Unsatisfactory therapeutic effects of tofacitinib are observed in this study and further evaluations are needed.

14.
Cell Biochem Biophys ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31696435

RESUMO

Candidate oncogene placenta specific 8 (PLAC8) has been identified to participate in different cellular process and human diseases. However, the effects of PLAC8 on cell proliferation and migration in human kidney cancer (KC) remained unclear. In current study, physiological effects of PLAC8 in immortalized human embryonic kidney cell line (HEK293T) were investigated in vitro. Two PLAC8 knockout (KO) cell lines were established via CRISPR/Cas9-mediated methods combined with fluorescence activated single cell sorting. To classify the characteristic of PLAC8 during cell proliferation and migration in HEK293T, cellular proliferative activity was analyzed by cell counting and colony formation assay. Cell cycle distribution was analyzed by flow cytometry. Cellular motile activity was analyzed by wound-healing and migration assay. Further underlying molecular mechanism was explored via western blot. With the KO cell lines, it was found that PLAC8 KO could decrease cell proliferation. Moreover, the inhibitory effects of PLAC8 KO on cell proliferation were associated with a G2/M arrest in cell cycle progression concomitant with a remarkable inhibition of Cyclin B1 and elevation of Cyclin A. The alteration of cell cycle proteins and E-cadherin might further associate with the enhancement of cell motility. Our study revealed a novel role for PLAC8 in cell proliferation and migration of HEK293T cells, which might shed light on further study of PLAC8 on human KC.

15.
Clin Exp Nephrol ; 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31605314

RESUMO

BACKGROUND: Crescent formation in immunoglobulin A nephropathy (IgAN) has been demonstrated to be a risk factor for worse outcomes. For IgAN patients with 0-25% crescentic glomeruli (C1), whether corticosteroids (CS) can improve the prognosis remains unclear. We tried to investigate the need for using CS in IgAN patients with C1 in different proteinuria levels. METHODS: A total of 120 eligible IgAN patients with C1 from two academic medical centers were retrospectively studied, and 57 (47.5%) received CS. Patients were grouped according to with or without CS. The outcomes were the rate of estimated glomerular filtration rate (eGFR) decline (ml/min per 1.73 m2/year) and a composite outcome (50% decrease in eGFR, end stage renal disease (ESRD) or death due to kidney disease). The progression of adverse outcome among them were analyzed in Kaplan-Meier curve. The independent significance of CS on renal outcome or eGFR decline rate were analyzed by multivariable Cox regression or linear regression. RESULTS: Unadjusted Kaplan-Meier showed that the outcome of treated patients was better than that of the untreated patients. Multiple Cox regression and linear regression analysis found that CS independently protected the renal outcome and decreased the eGFR decline rate. In the subgroup analysis, multivariate linear regression showed that CS decreased the eGFR decline rate both in proteinuria ≥ 1 g/day and < 1 g/day. CONCLUSIONS: CS protected the renal outcome and slowed the eGFR decline rate of IgAN patients with C1, it also decreased the eGFR decline rate even in those with initial proteinuria < 1 g/day.

16.
Inorg Chem ; 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31652061

RESUMO

The research on exploring advanced electrocatalysts that coupled with structural coherence and fast mass/electron transport characteristics, and maximized electrocatalytic redox activity is extremely urgent for the oxygen evolution reaction (OER), a key process for water dissociation, but it still challenging. Herein, we demonstrate a templated-engaged strategy for the fabrication of highly open and defect-rich Mn-doped Cu(OH)2 hexagonal nanorings (denoted as Mn-doped Cu(OH)2 HNs) by employing Mn(OH)2 hexagonal nanoplates as a sacrificial template. As a result of the successful doping of Mn into Cu(OH)2, the as-prepared Mn-doped Cu(OH)2 HNs possess rich defects and a modified electronic structure, which contribute to the exceptional property as a catalyst for OER electrocatalysis. More importantly, by coupling nickel foam (NF) supported Mn-doped Cu(OH)2 HNs as the anode electrode, NFs supported Pt/C as cathode electrode, a potential of only 1.62 V is needed to drive the water electrolysis to reach the current density of 10 mA cm-2, comparable to the commercial IrO2//Pt/C couple.

17.
Plant Physiol Biochem ; 144: 254-263, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31593898

RESUMO

To elucidate the mechanism of soybean resistance to Al, physiological and biochemical indices and antioxidant enzyme expression and activities were systematically analyzed in Al-sensitive (Glycine max Merr., Yunnan Province of China, SB) and Al-resistant Dambo (Glycine max Merr., Kyoto of Japan, RB) black soybean plants. According to the results, the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in RB root tips were significantly lower than those in SB root tips, though the opposite results occurred for soluble protein contents. Moreover, the expression and activities of superoxide dismutase (SOD, EC1.15.1.1.1.1.1.1), peroxidase (POD, EC1.11.1.7) and catalase (CAT, EC1.11.1.6) under 0-400 µM Al for 0-96 h were greater in RB than in SB. However, below 100 µM Al, the activities of those enzymes in SB increased with increasing Al concentration and treatment duration, with SOD activity being lowest and CAT activity exceeding that of POD with increasing Al concentration. Overall, enzyme activity in SB treated with Al at concentrations greater than 200 µM was lower than that in the SB control (CK; not treated with Al) and decreased with treatment duration. Additionally, at Al concentrations lower than 200 µM, enzyme activities in RB were significantly greater than those in RB CK and increased with both Al concentration and treatment duration. Moreover, enzyme activity in RB treated with 400 µM Al was slightly greater than that in RB CK. Thus, CAT activity determines soybean resistance to Al. These results indicate that soybean resistance to Al can be enhanced by regulating the expression and activity of antioxidant enzymes to remove H2O2 under Al stress.

18.
BMC Public Health ; 19(1): 1404, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31664970

RESUMO

BACKGROUND: Skin disease is a global public health problem that often has physiological, psychological and social impacts. However, it is not very clear how to adapt to these impacts, especially psychosocial adaptation of patients with skin disease. METHODS: We searched EMBASE, PubMed, CINAHL and PsycINFO from 2009 to 2018. The following themes were extracted from the included articles: the concepts, related factors, and interventions for psychosocial adaptation of patients with skin disease. Two reviewers independently screened and analyzed. RESULTS: From 2261 initial records, 69 studies were identified and analyzed. The concept of psychosocial adaptation in patients with skin disease was referred to under an assortment of descriptions. The related factors for psychosocial adaptation in patients with skin disease included the following: demographic factors (sex, age, education level, ethnicity, BMI, sleep quality, marital status, exercise amount, family history, the use of topical treatment only, personality and history of smoking); disease-related factors (disease severity, clinical symptoms, localization and duration); psychological factors (anxiety/depression, self-esteem, body image, stigma and suicidal ideation); and social factors (social support, social interaction, sexual life, economic burden and social acceptance). Despite being limited in quantity, several studies have clarified the benefits of adjuvant care in the form of cognitive behavioral training, educational training and self-help programs, all of which have become common methods for dealing with the psychosocial impacts. CONCLUSIONS: Based on the previous literatures, we constructed a protocol of care model for psychosocial adaptation in patients with skin disease. It not only provided the direction for developing new instruments that could assess psychosocial adaptation statue, but also a basis for helping patients adjust to changes in skin disease.

19.
Mol Imaging Biol ; 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31659574

RESUMO

PURPOSE: This work aims to identify intratumoral habitats with distinct heterogeneity based on 2-deoxy-2-[18F]fluro-D-glucose positron emission tomography (PET)/X-ray computed tomography (CT) imaging, and to develop a subregional radiomics approach to predict progression-free survival (PFS) in patients with nasopharyngeal carcinoma (NPC). PROCEDURES: In total, 128 NPC patients (85 vs. 43 for primary vs. validation cohorts) who underwent pre-treatment PET/CT scan were enrolled retrospectively. Each tumor was partitioned into several phenotypically consistent subregions based on individual- and population-level clustering. For each subregion, 202 radiomics features were extracted to construct imaging biomarker for prognosis via Cox's proportional hazard model combined with forward stepwise feature selection. Relevance of imaging biomarkers and clinicopathological factors were assessed by multivariate Cox regression analysis and Spearman's correlation analysis. To investigate whether imaging biomarkers could provide complementary prognosis information beyond existing predictors, a scoring system was further developed for risk stratification and compared with AJCC staging system. RESULTS: Three subregions (denoted as S1, S2, and S3) were discovered with distinct PET/CT imaging characteristics in the two cohorts. The prognostic performance of imaging biomarker S3 outperformed the whole tumor (C-index, 0.69 vs. 0.58; log-rank test, p < 0.001 vs. p = 0.552). Imaging biomarker S3 and AJCC stage were identified as independent predictors (p = 0.011 and 0.042, respectively) after adjusting for clinicopathological factors. The scoring system outperformed the traditional AJCC staging system (log-rank test, p < 0.0001 vs. p = 0.0002 in primary cohort and p = 0.0021 vs. p = 0.0277 in validation cohort, respectively). CONCLUSIONS: Subregional radiomics analysis of PET/CT imaging has the potential to predict PFS in patients with NPC, which also provides complementary prognostic information for traditional predictors.

20.
Curr Neurovasc Res ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31660818

RESUMO

OBJECTIVE: This study was to investigate the potential protective effects of curcumin in cerebral ischemia-reperfusion (CIR) and its regulation of miR-7. METHODS: Rats were occluded by middle cerebral artery occlusion (MCAO) for 1.5 h and reperfused for 2 h to establish a local CIR model. After 24 hours of model establishment, MCAO rats were given curcumin for 3 days by intragastric administration. PC12 cells were cultured for 6 h in oxygen-glucose deprivation medium and then reoxygenated for 24 h to establish an oxygen-glucose deprivation/reoxygenation (OGD/R) model. The OGD/R model cells were treated with curcumin for 48 h. RESULTS: Curcumin inhibited the decrease of miR-7-5p expression and increase of RelA p65 expression induced by CIR and ODG/R. RelA p65 was a target of miR-7-5p. MiR-7-5p antagonists were able to counteract the effect of curcumin on the expression of RelA p65 in ischemic brain tissue of MCAO rats and OGD/R model cells. Curcumin improved OGD/R-induced inhibition of cell activity, necrosis and apoptosis. Curcumin significantly reduced the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1ß, reactive oxygen species (ROS) and malondialdehyde (MDA) and increased the activity of superoxide dismutases (SOD) and catalase (CAT) in OGD/R-induced cells. Curcumin may inhibit OGD/R-induced cell damage by regulating miR-7-5p. Curcumin improved cerebral infarction, nerve damage and cognitive dysfunction in rats with CIR, which may be related to the regulation of miR-7-5p/RelA p65 axis. CONCLUSION: Curcumin exerts cerebral protection by attenuating cell necrosis and apoptosis, inflammatory response and oxidative stress following CIR, which may be related to its regulation of the miR-7/RELA p65 axis.

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