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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121873, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36126624

RESUMO

In this work, a rapid and sensitive strategy was developed to determine thiram (THI) and atrazine (ATZ) by surface-enhanced Raman scattering (SERS) technology. ß-cyclodextrin modified silver nanoparticles (ß-CD-AgNPs) were synthesized using ß-CD as a reducing agent and encapsulating agent under alkaline conditions and employed as SERS substrate. The existence of ß-CD can capture the molecules to form host-guest complex and fix molecular orientation in its cavity, thus ensuring the enhanced SERS signal intensity of THI and ATZ. The linear response extends from 2.56 × 10-8 to 2.56 × 10-3 mol/L for THI and 3.08 × 10-8 to 3.08 × 10-3 mol/L for ATZ, with the limits of detection (LOD) of 2.42 × 10-9 mol/L for THI and 7.26 × 10-9 mol/L for ATZ, respectively. The application of the proposed method in real samples including apple and water were investigated, and the results would help promote the application of SERS technology as a powerful analytical tool for detecting other pesticide residues. It is expected that this SERS strategy will provide great value for rapid detecting pesticide residues in food products and environmental systems.


Assuntos
Atrazina , Nanopartículas Metálicas , Resíduos de Praguicidas , Tiram/análise , Análise Espectral Raman/métodos , Resíduos de Praguicidas/análise , Atrazina/análise , Nanopartículas Metálicas/química , Frutas/química , Prata/química , Água/análise
2.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121872, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36152504

RESUMO

Small molecular food contaminants, such as mycotoxins, pesticide residues and antibiotics, are highly probable to be passively introduced in food at all stages of its processing, including planting, harvest, production, transportation and storage. Owing to the high risks caused by the unknowing intake and accumulation in human, there is an urgent need to develop rapid, sensitive and efficient methods to monitor them. Fluorescence-based aptasensors provide a promising platform for this area owing to its simple operation, high sensitivity, wide application range and economical practicability. In this paper, the common sorts of small molecular contaminants in foods, namely mycotoxins, pesticides, antibiotics, etc, are briefly introduced. Then, we make a comprehensive review, from fluorescence resonance energy transfer (in turn-on, turn-off, and ratiometric mode, as well as energy upconversion) to fluorescence polarization, of the fluorescence-based aptasensors for the determination of these food contaminants reported in the last five years. The principle of signal generation, the advances of each sort of fluorescent aptasensors, as well as their applications are introduced in detail. Additionally, we also discussed the challenges and perspectives of the fluorescent aptasensors for small molecular food contaminants. This work will offer systematic overview and inspiration for amateurs, researchers and developers of fluorescence-based aptasensors for the detection of small molecules.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Micotoxinas , Humanos , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Transferência Ressonante de Energia de Fluorescência , Antibacterianos
3.
J Hazard Mater ; 444(Pt A): 130406, 2023 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-36417778

RESUMO

People constantly move their heads during conversation, as such movement is an important non-verbal mode of communication. Head movement alters the direction of people's expired air flow, therefore affecting their conversational partners' level of exposure. Nevertheless, there is a lack of understanding of the mechanism whereby head movement affects people's exposure. In this study, a dynamic meshing method in computational fluid dynamics was used to simulate the head movement of a human-shaped thermal manikin. Droplets were released during the oral expiration periods of the source manikin, during which it was either motionless, was shaking its head or was nodding its head, while the head of a face-to-face target manikin remained motionless. The results indicate that the target manikin had a high level of exposure to respiratory droplets when the source manikin was motionless, whereas the target manikin's level of exposure was significantly reduced when the source manikin was shaking or nodding its head. The source manikin had the highest level of self-exposure when it was nodding its head and the lowest level of self-exposure when its head was motionless. People's level of exposure during close contact is highly variable, highlighting the need for further investigations in more realistic conversational scenarios.


Assuntos
Líquidos Corporais , Movimentos da Cabeça , Humanos , Aerossóis e Gotículas Respiratórios , Comunicação , Expiração
4.
Sci Total Environ ; 856(Pt 2): 159180, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36191704

RESUMO

Microplastic (MPs) pollution is a global marine environmental problem. The effects of MPs on the gut microbiota of aquatic organisms have received considerable attention. For example, microbes colonizing MPs in pond cultures alter the structure and function of the intestinal microbes of shrimp and fish. It was hypothesized that bacteria on MPs in natural mariculture areas also interact with the intestinal flora of golden pompano (Trachinotus ovatus) because biofilms can form on the surface of MPs during long-term floating in seawater. To our knowledge, this study is the first to investigate MPs pollution in T. ovatus aquaculture. DNA sequencing and bioinformatics analysis confirmed the effect of microbial colonization of MPs on the intestinal flora of T. ovatus. The MPs detected in the gut wet weight (w.w.) of golden pompano (546 ± 52 items/g) were mainly pellets and fragments of blue or green, whereas the sediment MPs dry weight (d.w.) (4765 ± 116 items/kg) were mainly black fibers. The MPs richness in the sediment gradually increased from the open-sea aquaculture area to the estuarine aquaculture area and was positively correlated with the MPs richness in the intestinal tract of golden pompano. MPs 20-200 µm were the most common in the gut and sediment. The intake of MPs increased the abundance of Proteobacteria and decreased that of Firmicutes in the intestinal flora. The functional compositions of MP-colonizing microbes and gut microbiota were similar, suggesting that the two communities influence each other. Network analysis further confirmed this and revealed that Vibrio plays a key role in the intestinal flora and surface microorganisms of MPs. Overall, the intake of MPs by aquatic animals not only affects the intestinal flora and intestinal microbial function, but also poses potential risks to aquaculture.


Assuntos
Microbioma Gastrointestinal , Vibrio , Animais , Microplásticos , Plásticos , Aquicultura , Peixes
5.
J Affect Disord ; 322: 39-45, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36375541

RESUMO

BACKGROUND: Anxious depression is a common subtype of major depressive disorder (MDD) associated with adverse outcomes and severely impaired social function. The aim of this study was to explore the relationships between child maltreatment, family functioning, social support, interpersonal problems, dysfunctional attitudes, and anxious depression. METHODS: Data were collected from 809 MDD patients. The Hamilton Anxiety Scale (HAMA), Hamilton Depression Scale-17 (HAMD-17), Family Assessment Device (FAD), Childhood Trauma Questionnaire (CTQ), Social Support Rating Scale (SSRS), Interpersonal Relationship Integrative Diagnostic Scale (IRIDS), and Dysfunctional Attitudes Scale (DAS) were administered and recorded. Anxious depression was defined as an anxiety/somatization factor score ≥ 7 on the HAMD-17. Chi-squared tests, Mann-Whitney U tests, distance correlations, and structural equation models were used for data analysis. RESULTS: Two-fifths of MDD patients had comorbid anxiety, and there were significant differences in child maltreatment, family functioning, social support, interpersonal problems, and dysfunctional attitudes between groups. Of these factors, interpersonal relationships were most related to anxiety in MDD patients, and dysfunctional attitudes mediated the relationship between interpersonal relationships and anxiety in MDD patients. LIMITATIONS: This study used cross-sectional data with no further follow-up to assess patient outcomes. This study did not include information about pharmacological treatments. A larger sample size is needed to validate the results. CONCLUSIONS: Psychosocial factors were significantly associated with anxious depression. Interpersonal relationships and dysfunctional attitudes have a direct effect on anxious depression, and interpersonal relationships also mediate the effects of anxious depression via dysfunctional attitudes.


Assuntos
Depressão , Transtorno Depressivo Maior , Criança , Humanos , Transtorno Depressivo Maior/psicologia , Estudos Transversais , Transtornos de Ansiedade/psicologia , Ansiedade/epidemiologia , Ansiedade/psicologia
6.
Curr Probl Cardiol ; : 101527, 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36455793

RESUMO

PD-1(programed death-1)/PD-L1(programed death-1 ligand) blockade represents a major breakthrough of anti-cancer therapies, however, it may come with increased risk of cardiovascular morbidity, such as myocarditis, acute coronary syndrome, arrhythmias, etc. Although the PD-1/PD-L1 blockade-related acute coronary syndrome (ACS) is rare, it can be fatal. Previous studies have implicated a role of the PD-1/PD-L1 axis in the development of atherosclerosis. This review explores a hypothesis that PD-1/PD-L1 blockade accelerates the progression of atherosclerosis and promotes plaque rupture, by synthesizing the evidence of vascular inflammation, as well as plaque progression, destabilization and rupture via T-cell activation and effector function. In order to improve the prognosis of cancer patients and decrease the cardiotoxicity of PD-1/PD-L1 blockade therapy, early recognition of PD-1/PD-L1-blockade-related ACS is important.

7.
Psych J ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36455922

RESUMO

Based on the conservation of resources theory, this two-wave study investigated the mediating role of work-nonwork conflict in the relationship between job crafting and employee recovery experience and examined the moderating role of work demands in this relationship. Participants were 486 employees (39.3% male and 60.7% female) from a medical company in the central region of China who responded to a paper-and-pencil survey twice with a 1-month interval. Regression-based results indicated that job crafting positively predicted recovery experience after work through lower work-nonwork conflict. Furthermore, the association between job crafting and work-nonwork conflict was moderated by work demands, such that the effect was stronger for employees with higher work demands. The present study explains how job crafting may improve employees' after-work recovery experience and addresses whether this process could be more significant for employees with higher work demands. The conclusion has practical implications for improving employee recovery experience.

8.
J Hazard Mater ; 445: 130526, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36463741

RESUMO

Agrochemicals such as pesticide residues become environmental contaminants due to their ecotoxic risks to plant, animal and human health. Ametryn (AME) is a widely used farmland pesticide and its residues are widespread in soils, surface stream and groundwater. However, its toxicological and degradative mechanisms in plants and food crops are largely unknown. This study comprehensively investigated AME toxicology and degradation mechanisms in a paddy crop. AME was freely absorbed by rice roots, translocated to the above-ground and thus repressed plant elongation, and reduced dry weight and chlorophyll concentration, but increased oxidative injury and subcellular electrolyte permeability. Analysis of the transcriptome and metabolome revealed that exposure to AME evoked global AME-responsive genes and step-wise catabolism of AME. We detected 995 (roots) and 136 (shoots) upregulated and differentially expressed genes (DEGs) in response to AME. Metabolomic profiling revealed that many basal metabolites such as carbohydrates, amino acids, glutathione, hormones and phenylpropanoids involved in AME catabolism were accordingly accumulated in rice. Eight metabolites and twelve conjugates of AME were characterized by HPLC-Q-TOF-HRMS/MS. These AME metabolites and conjugates are closely related to DEGs, differentially accumulated metabolites (DAMs) and activities of antioxidative enzymes. Collectively, our work highlights the specific mechanisms for AME degradative metabolism through Phase I and II reactive pathways (e.g. hydroxylation and dealkylation), with will help develop genetically engineered rice used to bioremediate AME-contaminated paddy soils and minimize AME accumulation rice crops.

9.
Brain Res ; : 148192, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36463959

RESUMO

BACKGROUND: Hypoxia-ischemic brain damage (HIBD) is a primary cause of morbidity and disability in survivors of preterm infants. We previously discovered that miR-200b-3p plays an important role in HIBD via targeting Slit2. This study was designed to identify novel targets of miR-200b-3p and investigate the relationship between miR-200b-3p and its downstream effectors. METHODS AND RESULTS: Cultured primary rat hippocampal neurons were used in the model of oxygen-glucose deprivation (OGD) and RT-qPCR was utilized to detect the alterations of miR-200b-3p in these cells following the OGD. Our study found that the expression of miR-200b-3p was up-regulated in neurons post OGD. Bioinformatics analysis identified that ß transducin repeat-containing protein (ß-TrCP) is a target gene of miR-200b-3p, and our luciferase reporter gene assay confirmed that miR-200b-3p can interact with ß-TrCP mRNA. Hypoxia-ischemic brain damage was induced in three-day-old SD rats and inhibition of miR-200b-3p by injection of antagomir into bilateral lateral ventricles enhanced ß-TrCP expression at both the mRNA and protein levels in rats' brains. TUNEL staining and CCK-8 assays found that the survival of hippocampal neurons in the miR-200b-3p antagomir group was improved significantly (p<0.05), whereas apoptosis of neurons in the miR-200b-3p antagomir group was significantly decreased (p<0.05), as compared with the OGD group. However, silencing of ß-TrCP by ß-TrCP siRNA impaired the neuroprotective effect of miR-200b-3p antagomir. H&E staining showed that miR-200b-3p attenuated the pathological changes in the hippocampal region of rats with HIBD. CONCLUSION: Our study has demonstrated that ß-TrCP is a target gene of miR-200b-3p and that inhibition of miR-200b-3p by antagomir attenuates hypoxia-ischemic brain damage via ß-TrCP.

10.
Eur J Pharmacol ; : 175430, 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36460131

RESUMO

Disruption of blood-testis barrier (BTB) was a crucial pathological feature of diabetes induced-testicular injury at early phase. Aucubin (AU), a main active component in Eucommiae Cortex, has drawn attention for its benefits against male reproductive system disease. The current study was aimed at investigating the protective role of AU and exploring the underlying mechanism in diabetic model. A murine model of type 2 diabetes mellitus (T2DM) was induced by high-fat diet (HFD) combined with streptozocin (STZ). Testicular weight index and morphology, sperm quality, integrity of BTB and protein levels were analyzed. The underlying mechanism of the protective effect of AU was further explored in Sertoli cells (SCs) cultured with high glucose (HG). Our results showed AU inhibited testicular structural destruction, restored disruption of BTB and improved abnormal spermatogenic function in diabetic mice. Consistent with in vivo results, HG induced decreased transcellular resistance and increased permeability in SCs monolayers, while AU exposure reverses this trend. Meanwhile, reduced expression of Zonula occludin-1(ZO-1) and Connexin43(Cx43) in testicular tissue diabetic mice and HG-induced SCs was prominently reversed via AU treatment. Mechanistic studies suggested a high affinity interaction between AU and c-Src protein was identified based on molecular docking, and the activation of c-Src was significantly inhibited in AU treatment. Furthermore, AU significantly increased the expression of Cx43 and ZO-1 proteins HG-induced SCs, which can be further enhanced in gene-silenced c-Src cells to some extent. Our results suggested that AU ameliorated disruption of BTB and spermatogenesis dysfunction in diabetic mice via inactivating c-Src to stabilize cell junction integrity.

11.
Front Immunol ; 13: 1066936, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466908

RESUMO

As the precursor of taurine, cysteine serves physiological functions, such as anti-oxidative stress and immune improvement. Investigation of cysteine and its derivatives has made positive progress in avian and mammalian species, yet the study and application of cysteine in aquatic animals are relatively rare. Therefore, we evaluated the effects of supplementing a low-fishmeal diet with various levels of cysteine on the growth, antioxidant capacity, intestine immunity, and resistance against Streptococcus agalactiae of the juvenile golden pompano (Trachinotus ovatus). According to our study, exogenous supplementation with 0.6-1.2% cysteine greatly increased the final body weight (FBW) and specific growth rate (SGR) of golden pompano compared to the control group. Under the present conditions, the optimum dietary cysteine supplementation level for golden pompano was 0.91% based on the polynomial regression analysis of SGR. Meanwhile, we found that the Nrf2/Keap1/HO-1 signaling pathway was notably upregulated with the increase of exogenous cysteine, which increased antioxidant enzyme activity in serum and gene expression in the intestine and reduced the level of reactive oxygen species (ROS) in the serum of golden pompano. In addition, morphological analysis of the midgut demonstrated that exogenous cysteine improved muscle thickness and villi length, which suggested that the physical barrier of the intestine was greatly strengthened by cysteine. Moreover, cysteine increased the diversity and relative abundance of the intestinal flora of golden pompano. Cysteine suppressed intestinal NF-κB/IKK/IκB signaling and pro-inflammatory cytokine mRNA levels. Conversely, intestinal anti-inflammatory cytokine gene expression and serum immune parameters were upregulated with the supplementary volume of cysteine and improved intestine immunity. Further, exogenous cysteine supplementation greatly reduced the mortality rate of golden pompano challenged with S. agalactiae. In general, our findings provide more valuable information and new insights into the rational use of cysteine in the culture of healthy aquatic animals.


Assuntos
Cisteína , Streptococcus agalactiae , Animais , Cisteína/farmacologia , Proteína 1 Associada a ECH Semelhante a Kelch , Antioxidantes/farmacologia , Fator 2 Relacionado a NF-E2 , Peixes , Intestinos , Dieta/veterinária , Estresse Oxidativo , Citocinas , Mamíferos
12.
Comput Struct Biotechnol J ; 20: 6543-6551, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36467579

RESUMO

The world's population continues to increase and thus requires more food production to take place in nonarable land, such as saline soil; therefore, it is urgent to find solutions to enhance the salinity tolerance of crops. As the second genome of plants, the rhizosphere microbiome plays critical roles in plant fitness under stress conditions. Many beneficial microbes that help plants cope with salinity stress have been identified, highlighting their roles in mitigating salt stress-induced negative effects on plants. However, a comprehensive review of the microbial species that are able to confer plant salt tolerance and the underlying mechanisms is still lacking. In this review, we compared the representative fungal and bacterial taxa that demonstrate the ability to enhance plant growth in saline soil. We also reviewed the mechanisms by which rhizosphere microbes enhance plant salt stress tolerance, i.e., by re-establishing ion and osmotic homeostasis, preventing damage to plant cells, and resuming plant growth under salt stress. Finally, future research efforts to explore the rhizosphere microbiome for agricultural sustainability are proposed.

13.
Childs Nerv Syst ; 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36471063

RESUMO

PURPOSE: Pineal anlage tumor is an extremely rare tumor which was considered as a subtype of pineovlatoma with an overall poor prognosis. This case-based review further summarize the clinical profile. METHODS: A patient with pineal anlage tumor was reported, her clinical data and gene analysis results were recorded. RESULTS: An 8-month-old girl, with an obvious enhancing pineal occupancy and obstructive hydrocephalus. Her histological and immunohistochemical findings contained rhabdomyoblastic, melanin pigment and cartilage island. The wholeexpme sequencing and genome-wide copy number variation sequencing were performed, no mutations associated with pineoblatoma as well as copy number variants were identified. In terms of treatment, our patient underwent subtotal resection without radiotherapy or chemotherapy, and the residual tumor enlarged 4 months after surgery. We have followed her up for 10 months, and the child is still alive. CONCLUSION: Surgery combined radiotherapy and chemotherapy is still the best treatment currently,and genetic testing for patients is necessary.

14.
J Pharm Anal ; 12(5): 791-800, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36320600

RESUMO

In this study, ecofriendly and economic carboxy-terminated plant fibers (PFs) were used as adsorbents for the effective in-syringe solid phase extraction (IS-SPE) of fluoroquinolone (FQ) residues from water. Based on the thermal esterification and etherification reaction of cellulose hydroxy with citric acid (CA) and sodium chloroacetate in aqueous solutions, carboxy groups grafted onto cotton, cattail, and corncob fibers were fabricated. Compared with carboxy-terminated corncob and cotton, CA-modified cattail with more carboxy groups showed excellent adsorption capacity for FQs. The modified cattail fibers were reproducible and reusable with relative standard deviations of 3.2%-4.2% within 10 cycles of adsorption-desorption. A good extraction efficiency of 71.3%-80.9% was achieved after optimizing the extraction condition. Based on carboxylated cattail, IS-SPE coupled with ultra-performance liquid chromatography with a photodiode array detector was conducted to analyze FQs in environmental water samples. High sensitivity with limit of detections of 0.08-0.25 µg/L and good accuracy with recoveries of 83.8%-111.7% were obtained. Overall, the simple and environment-friendly modified waste PFs have potential applications in the effective extraction and detection of FQs in natural waters.

15.
Artigo em Inglês | MEDLINE | ID: mdl-36333998

RESUMO

Brain functional alterations in type 2 diabetes with diabetic peripheral neuropathy (DPN) related to motor dysfuction remain largely unknown. We aim to explore intrinsic resting brain activity in DPN. A total of 28 DPN patients, 43 diabetics without DPN (NDPN) and 32 healthy controls (HCs) were recruited and underwent resting-state functional MRI. We calculated the amplitude of low-frequency fluctuation (ALFF), fractional ALFF (fALFF) and regional homogeneity (ReHo). One-way analysis of covariance was applied to evaluate above indicators among the three groups, and the mean ALFF/fALFF/ReHo values of altered brain regions were then correlated to clinical features of patients. Compared with NDPN group, the DPN group showed significantly decreased ALFF values in the right orbital superior frontal gyrus (ORBsup), medial superior frontal gyrus (SFGmed) and incresead ALFF values in the left inferior temporal gyrus (ITG) and decreased fALFF values in the right SFGmed. Compared with HCs, the NDPN group showed increased ALFF values in the right ORBsup, middle frontal gyrus, left orbital middle frontal gyrus and decreased fALFF values in the right middle temporal gyrus. Notably, the mean ALFF values of the right ORBsup were significantly negatively correlated with Toronto Clinical Scoring System scores and gait speed in diabetics. The mean ALFF/fALFF values of right SFGmed, the mean ALFF values of left ITG and right ORBsup were significantly differentiated between DPN and NDPN patients in ROC curve analysis. DPN patients have abnormal brain activity in sensorimotor and cognitive brain areas, which may implicate the underlying neurophysiological mechanisms in intrinsic brain activity.

16.
Ther Adv Hematol ; 13: 20406207221132346, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36324489

RESUMO

Background: The clinical risk classification of acute myelocytic leukemia (AML) is largely based on cytogenetic and molecular genetic detection. However, the optimal treatment for intermediate-risk AML patients remains uncertain. Further refinement and improvement of prognostic stratification are therefore necessary. Objectives: The aim of this study was to identify serum protein biomarkers to refine risk stratification in AML patients. Design: This study is a retrospective study. Methods: Label-free proteomics was used to identify the differential abundance of serum proteins in AML patients. Transcriptomic data were combined to identify key altered markers that could indicate the risk rank of AML patients. The survival status was assessed by Kaplan-Meier and multivariate Cox regression analyses. Results: We delineated serum protein expression in a population of AML patients. Many biological processes were influenced by the identified differentially expressed proteins. Association analysis of transcriptome data showed that intercellular adhesion molecule-2 (ICAM2) had a higher survival prediction value in the intermediate-risk AML group. ICAM2 was detrimental for intermediate-risk AML, regardless of whether patients received bone marrow transplantation. ICAM2 well distinguishes the intermediate group of patients, whose probability of survival is comparable to that of patients with the ELN-2017 according to the reference classification. In addition, newly established stratified clinical features were associated with leukemia stem cell scores. Conclusion: The inclusion of ICAM2 expression into the AML risk classification according to ELN-2017 was a good way to transfer patients from three to two groups. Thus, providing more information for clinical decision-making to improve intermediate-risk stratification in AML patients.

17.
Front Immunol ; 13: 982628, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36325340

RESUMO

Background: Prostate cancer (PCa), a prevalent malignant cancer in males worldwide, screening for patients might benefit more from immuno-/chemo-therapy remained inadequate and challenging due to the heterogeneity of PCa patients. Thus, the study aimed to explore the metabolic (Meta) characteristics and develop a metabolism-based signature to predict the prognosis and immuno-/chemo-therapy response for PCa patients. Methods: Differentially expressed genes were screened among 2577 metabolism-associated genes. Univariate Cox analysis and random forest algorithms was used for features screening. Multivariate Cox regression analysis was conducted to construct a prognostic Meta-model based on all combinations of metabolism-related features. Then the correlation between MetaScore and tumor was deeply explored from prognostic, genomic variant, functional and immunological perspectives, and chemo-/immuno-therapy response. Multiple algorithms were applied to estimate the immunotherapeutic responses of two MeteScore groups. Further in vitro functional experiments were performed using PCa cells to validate the association between the expression of hub gene SLC17A4 which is one of the model component genes and tumor progression. GDSC database was employed to determine the sensitivity of chemotherapy drugs. Results: Two metabolism-related clusters presented different features in overall survival (OS). A metabolic model was developed weighted by the estimated regression coefficients in the multivariate Cox regression analysis (0.5154*GAS2 + 0.395*SLC17A4 - 0.1211*NTM + 0.2939*GC). This Meta-scoring system highlights the relationship between the metabolic profiles and genomic alterations, gene pathways, functional annotation, and tumor microenvironment including stromal, immune cells, and immune checkpoint in PCa. Low MetaScore is correlated with increased mutation burden and microsatellite instability, indicating a superior response to immunotherapy. Several medications that might improve patients` prognosis in the MetaScore group were identified. Additionally, our cellular experiments suggested knock-down of SLC17A4 contributes to inhibiting invasion, colony formation, and proliferation in PCa cells in vitro. Conclusions: Our study supports the metabolism-based four-gene signature as a novel and robust model for predicting prognosis, and chemo-/immuno-therapy response in PCa patients. The potential mechanisms for metabolism-associated genes in PCa oncogenesis and progression were further determined.


Assuntos
Neoplasias da Próstata , Masculino , Humanos , Prognóstico , Neoplasias da Próstata/genética , Neoplasias da Próstata/terapia , Neoplasias da Próstata/metabolismo , Microambiente Tumoral/genética , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Imunoterapia , Proteínas dos Microfilamentos/metabolismo , Proteínas Cotransportadoras de Sódio-Fosfato Tipo I
18.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 34(9): 991-994, 2022 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-36377457

RESUMO

Podoplanin (PDPN) is a small transmembrane mucin-like glycoprotein which is expressed on the surface of lymphatic endothelial cells, glomerular podocytes, type-I alveolar epithelial cells and some tumor cells. PDPN plays crucial function in variety of physiological and pathological processes such as embryonic development, immunoreaction, inflammation and cancer. C-type lectin-like receptor 2 (CLEC2) is mainly expressed on the platelet which specific ligand is PDPN. The interaction between PDPN and CLEC2 has received extensive attention. In this review, we summarized recent researches on the role of in sepsis and elaborated the possible mechanisms and some potential therapies for sepsis by targeting PDPN, which may provide theoretical basis for the mechanism and treatment of sepsis.


Assuntos
Podócitos , Sepse , Cricetinae , Animais , Especificidade de Anticorpos , Células CHO , Epitopos , Cricetulus , Células Endoteliais , Anticorpos Monoclonais , Glicoproteínas de Membrana
20.
Transl Psychiatry ; 12(1): 474, 2022 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-36357369

RESUMO

In major depressive disorder (MDD) patients, nonsuicidal self-injury (NSSI) is a common comorbidity, and it is important to clarify the underlying neurobiology. Here, we investigated the association of NSSI with brain function and structure in MDD patients. A total of 260 MDD patients and 132 healthy controls (HCs) underwent resting-state functional magnetic resonance imaging and three-dimensional T1-weighted structural scans. NSSI behaviour was assessed through interviews. Voxel-based morphometry analysis (VBM), regional homogeneity analysis (ReHo), functional connectome topology properties and network-based statistics were used to detect the differences in neuroimaging characteristics. Finally, the random forest method was used to evaluate whether these factors could predict NSSI in MDD. Compared with HCs, MDD patients with a history of NSSI showed significant right putamen grey matter volume (GMV), right superior orbital frontal cortex ReHo, left pallidum degree centrality, and putamen-centre function network differences. Compared to MDD subjects without NSSI, those with past NSSI showed significant right superior temporal gyrus (STG) GMV, right lingual gyrus ReHo, sigma and global efficiency, and cerebellum-centre function network differences. The right STG GMV and cerebellum-centre function network were more important than other factors in predicting NSSI behaviour in MDD. MDD patients with a history of NSSI have dysregulated spontaneous brain activity and structure in regions related to emotions, pain regulation, and the somatosensory system. Importantly, right STG GMV and cerebellar loops may play important roles in NSSI in MDD patients.


Assuntos
Transtorno Depressivo Maior , Comportamento Autodestrutivo , Humanos , Encéfalo , Lobo Temporal/patologia , Imageamento por Ressonância Magnética/métodos , Cerebelo , Neuroimagem , Comportamento Autodestrutivo/diagnóstico por imagem
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