Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.019
Filtrar
1.
J Hazard Mater ; 443(Pt B): 130214, 2023 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-36327837

RESUMO

Bisphenol A (BPA) is a toxic endocrine disruptor detected in various environments. Microbial metabolic/enzymatic degradation has been thought to be the main pathway for BPA attenuation in natural environments. In this study, we found that under visible light conditions, superoxide produced by bacteria was the main reason for the rapid removal of BPA, accounting for 57 % of the total removal rate. With visible light, the bacteria degraded BPA at a rate of 0.22 mg/L/d, and the total removal within 8 days reached 85 %, which is 4.7 times compared with that of dark culture. The intermediate product 4-iso-propenylphenol, which was considered as an end-product of microbial degradation of BPA in previous reports, was detected in large quantities at 24 h in culture but gradually decreased in our experiment. Community analysis suggested bacteria with aromatic hydrocarbon degradation ability were more enriched under light incubation. Moreover, the bacteria showed well degradation ability to various pharmaceutically active but nonbiodegradable compounds including diclofenac and fluoxetine, with a removal rate of 88 % and 20 %, respectively. Our study revealed the organic pollutant transformation pathway under the combined action of light and microorganisms, providing new insights into the microbial treatment of aromatic hydrocarbon pollutants.


Assuntos
Compostos Benzidrílicos , Poluentes Ambientais , Biodegradação Ambiental , Compostos Benzidrílicos/metabolismo , Fenóis/metabolismo , Bactérias/metabolismo , Luz
2.
Food Chem ; 404(Pt A): 134608, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36272301

RESUMO

Our previous studies have confirmed oligopeptides could be meaningful to tea taste and biofunction. As the total content of oligopeptide among different tea types were varied. It is a natural speculation that certain tea processing step could be vital for oligopeptides enrichment. In current study, five types of traditional Chinese tea were produced from the same batch of fresh leaves. Step processing samples were acquired through which to profile the oligopeptides and free amio acids composition change during tea processed. As a result, firstly, withering was the vital step for oligopeptide enrichment, followed by fermentation, yellowing and drying. Secondly, as the enrichment of oligopeptides was often accompanied by the increase of protein amino acids, suggesting certain proteins degradation in fresh leaves could be the main source of oligopeptides. Thirdly, a total of 166 abundant oligopeptides were screened out, through which 14 high degradation protein were also located by protomic approaching.


Assuntos
Camellia sinensis , Chá , Chá/química , Aminoácidos/análise , Fermentação , Camellia sinensis/química , Folhas de Planta/química , Oligopeptídeos/metabolismo
3.
Sci Total Environ ; 857(Pt 1): 159390, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36243072

RESUMO

Annual gross primary productivity (AGPP) is the basis for grain production and terrestrial carbon sequestration. Mapping regional AGPP from site measurements provides methodological support for analysing AGPP spatiotemporal variations thereby ensures regional food security and mitigates climate change. Based on 641 site-year eddy covariance measuring AGPP from China, we built an AGPP mapping scheme based on its formation and selected the optimal mapping way, which was conducted through analysing the predicting performances of divergent mapping tools, variable combinations, and mapping approaches in predicting observed AGPP variations. The reasonability of the selected optimal scheme was confirmed by assessing the consistency between its generating AGPP and previous products in spatiotemporal variations and total amount. Random forest regression tree explained 85 % of observed AGPP variations, outperforming other machine learning algorithms and classical statistical methods. Variable combinations containing climate, soil, and biological factors showed superior performance to other variable combinations. Mapping AGPP through predicting AGPP per leaf area (PAGPP) explained 86 % of AGPP variations, which was superior to other approaches. The optimal scheme was thus using a random forest regression tree, combining climate, soil, and biological variables, and predicting PAGPP. The optimal scheme generating AGPP of Chinese terrestrial ecosystems decreased from southeast to northwest, which was highly consistent with previous products. The interannual trend and interannual variation of our generating AGPP showed a decreasing trend from east to west and from southeast to northwest, respectively, which was consistent with data-oriented products. The mean total amount of generated AGPP was 7.03 ± 0.45 PgC yr-1 falling into the range of previous works. Considering the consistency between the generated AGPP and previous products, our optimal mapping way was suitable for mapping AGPP from site measurements. Our results provided a methodological support for mapping regional AGPP and other fluxes.


Assuntos
Mudança Climática , Ecossistema , Sequestro de Carbono , Solo , Aprendizado de Máquina , Carbono , Dióxido de Carbono/análise
4.
Talanta ; 251: 123770, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35961081

RESUMO

Iron oxide nanoparticles (IONPs) have been widely used as a nanoscale tool in biomedical research. However, it remains largely unknown how IONPs are transformed at a subcellular level to elicit distinct biological effects. In the present study, we prepared three different IONPs, including two IONPs targeting mitochondria (IONP-TPP) and lysosomes (IONP-APM), respectively, and a control with no specified target (IONP). By MTT assay and JC-1 staining, mitochondria-targeted IONP-TPP was found to produce significant cytotoxicity and severe mitochondrial membrane depolarization in MCF-7 cells. Furthermore, X-ray absorption spectroscopy (XAS) analysis revealed that IONP-TPP underwent remarkable edge defects and oxidation inside the cell. These findings suggest that IONPs are prone to the chemical transformation at mitochondria, and mitochondria are vulnerable to IONPs accumulation in the cell.


Assuntos
Nanopartículas de Magnetita , Nanopartículas , Compostos Férricos/toxicidade , Lisossomos , Nanopartículas de Magnetita/química , Mitocôndrias , Coloração e Rotulagem
5.
Cell Biol Int ; 46(3): 475-487, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34939719

RESUMO

Mutations of PSEN1 have been reported in dilated cardiomyopathy pedigrees. Understanding the effects and mechanisms of PSEN1 in cardiomyocytes might have important implications for treatment of heart diseases. Here, we showed that PSEN1 was downregulated in ischemia-induced failing hearts. Functionally, cardiovascular specific PSEN1 deletion led to spontaneous death of the mice due to cardiomyopathy. At the age of 11 months, the ratio of the heart weight/body weight was slightly lower in the Sm22a-PSEN1-KO mice compared with that of the WT mice. Echocardiography showed that the percentage of ejection fraction and fractional shortening was significantly reduced in the Sm22a-PSEN1-KO group compared with the percent of these measures in the WT group, indicating that PSEN1-KO resulted in heart failure. The abnormally regulated genes resulted from PSEN1-KO were detected to be enriched in muscle development and dilated cardiomyopathy. Among them, several genes encode Ca2+ ion channels, promoting us to investigate the effects of PSEN1 KO on regulation of Ca2+ in isolated adult cardiomyocytes. Consistently, in isolated adult cardiomyocytes, PSEN1-KO increased the concentration of cytosolic Ca2+ and reduced Ca2+ concentration inside the sarcoplasmic reticulum (SR) lumen at the resting stage. Additionally, SR Ca2+ was decreased in the failing hearts of WT mice, but with the lowest levels observed in the failing hearts of PSEN1 knockout mice. These results indicate that the process of Ca2+ release from SR into cytoplasm was affected by PSEN1 KO. Therefore, the abnormalities in Ca2+ homeostasis resulted from downregulation of PSEN1 in failing hearts might contribute to aging-related cardiomyopathy, which might had important implications for the treatment of aging-related heart diseases.


Assuntos
Cálcio , Cardiomiopatia Dilatada , Animais , Cardiomiopatia Dilatada/genética , Homeostase , Camundongos , Camundongos Knockout , Miócitos Cardíacos/fisiologia , Retículo Sarcoplasmático
6.
Med Oncol ; 40(1): 24, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36454423

RESUMO

As one of the most common malignant cancers in the world, gastric cancer is caused by mang factors among which tobacco smoke is an important risk factor. Gastric cancer stem cells (GCSCs) and the derived exosomes play a key role in the occurrence and development of gastric cancer, and exosomal circRNA is considered as a new regulatory factor in the development of gastric cancer. However, it is unclear whether tobacco smoke can affect exosomes and their transport circRNAs to promote the development of gastric cancer. Herein, we provided a new insight into tobacco smoke promoting the progression of gastric cancer. In the present study, we demonstrated that tobacco smoke-induced exosomes promoted the spheroidizing ability, stemness genes expression, and epithelial-mesenchymal transition (EMT) process of GCSCs. We further found that hsa-circRNA-000670 (circ670) was up-regulated in tissues of gastric cancer patients with smoking history, tobacco smoke-induced GCSCs, and their exosomes. Functional assays have shown that circ670 knockdown inhibited the stemness and EMT process of GCSCs, whereas circ670 overexpression appeared to have an opposite effect. Our findings indicated that exosomal circ670 promotes the development of tobacco smoke-induced gastric cancer, which may provide insight into the mechanism of tobacco smoke promoting the progression of gastric cancer.


Assuntos
Exossomos , Neoplasias Gástricas , Poluição por Fumaça de Tabaco , Humanos , RNA Circular/genética , Neoplasias Gástricas/genética , Tabaco/efeitos adversos , Células-Tronco Neoplásicas
7.
Bioresour Technol ; 369: 128426, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36462764

RESUMO

The high cost and severe foam in rhamnolipid fermentation are still bottlenecks for its industrial production and application. Non-foaming production of rhamnolipid by Pseudomonas aeruginosa FA1 was explored in solid-state fermentation using the agro-processing waste (peanut meal) as low-cost substrate. An environmental-friendly extraction method was developed to harvest rhamnolipid from solid-state culture. Strain FA1 produced 265.4 ± 8.2 mg rhamnolipid using 10 g peanut meal. HPLC-MS results revealed that 7 rhamnolipid homologues were produced, mainly including Rha-C8-C10 and Rha-Rha-C10-C10. Nitrate was the optimal nitrogen source. Peanut meal, MgSO4 and CaCl2 were significant factors for rhamnolipid production in solid-state fermentation. Rhamnolipid production was enhanced 31 % using the solid-state medium optimized by response surface method. The produced rhamnolipid reduced water surface tension to 28.1 ± 0.2 mN/m with a critical micelle concentration of 70 mg/L. The crude oil was emulsified with an emulsification index of 75.56 ± 1.29 %. The growth of tested bacteria and fungi was inhibited.

8.
Sci Total Environ ; : 160565, 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36464052

RESUMO

Fetal development is susceptible to various environmental stimuli, which possibly lead to lasting health effects. Of particular note is maternal environment that is increasingly recognized as an essential factor in fetal growth. However, the studies of the influences of environmental factors on fetal development are limited due to the species variation between animals and human as well as restricted source of human fetal tissues. Here, we construct a placental barrier-brain organoid-on-a-chip model, which enables to investigate the effects of maternal PM2.5 exposure on human fetal system including placental barrier and fetal brain. The multilayered microdevice is designed with the upper placental barrier and the bottom hiPSC-derived brain organoids, separated by porous membrane. This system represents multiple organs with physiological interactions between fetal and maternal side. Maternal PM2.5 exposure is simulated by introducing PM2.5 into the maternal side, and the subsequent effects are examined based on the multiorgan-on-a-chip (MOC) system. The results demonstrate that placental barrier is greatly injured with induced inflammatory responses. Moreover, brain organoids are potentially damaged including the impaired cell proliferation and cell differentiation, implying the adverse impacts of PM2.5 exposure on fetal neural development. These adverse responses caused by maternal PM2.5 exposure are potentially critical for the placental insufficiency, as well as abnormalities in fetal brain development. Together, human placental barrier-brain organoid-on-a-chip model presents a biomimetic fetal system to study the complex impacts of maternal environment on fetal development, and possibly advance our understanding of reproductive diseases and fetal disorders.

9.
Front Microbiol ; 13: 991703, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466641

RESUMO

Cryptococcus spp. is a complex species that often causes cryptococcosis, which is one of the most common opportunistic infections in adults living with HIV and has very high morbidity and mortality rates. This study aimed to investigate the antifungal susceptibility profiles and epidemiological characteristics of the Cryptococcus neoformans species complex (CNSC) and the Cryptococcus gattii species complex (CGSC) in Zhejiang Province, China. A total of 177 CNSC and 3 CGSC isolates were collected, and antifungal susceptibility was tested by FUNGUS 3 and verified with an E-test. Moreover, multiple classification methods and genomic analyses were performed. The majority of the isolates (96.11%) were C. neoformans (formerly C. neoformans var. grubii) (ST5-VNI-A-α). Our study highlights that most of the patients with cryptococcosis were non-HIV patients in China, and nearly half of them did not have underlying diseases that led to immune insufficiency. Most of the Cryptococcus spp. isolates in this study were sensitive to common antifungal drugs. Two 5-flucytosine (5-FC)-resistant strains were identified, and FUR1 mutation was detected in the 5-FC-resistant isolates. Typing based on whole-genome sequencing (WGS) showed better discrimination than that achieved with multilocus sequence typing (MLST) and indicated a clear population structure. A phylogenetic analysis based on WGS included more genomic information than traditional classification methods.

10.
Int Arch Allergy Immunol ; : 1-12, 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36470235

RESUMO

INTRODUCTION: Osteoarthritis (OA) is associated with oxidative stress injury (OSI) and inflammatory responses in chondrocytes. This study sought to explore the mechanism of high mobility group A1 (HMGA1) in interleukin-1beta (IL-1ß)-induced OSI and inflammatory responses in primary chondrocytes. METHODS: Primary chondrocytes were cultured and treated with IL-1ß to establish an OA-cell model. Levels of HMGA1, Jumonji domain-containing 3 (JMJD3), and ZEB1 in cells were determined by real-time quantitative polymerase chain reaction and Western blot analysis. Cell viability, contents of tumor necrosis factor-α, IL-6, and IL-10, reactive oxygen species level, and glutathione peroxidase activity were assessed by the cell counting kit-8 assay, enzyme-linked immunosorbent assay, and assay kits. Enrichment levels of HMGA1 on the JMJD3 promoter and enrichment levels of JMJD3 and trimethylated histone H3 at lysine 27 (H3K27me3) on the ZEB1 promoter region were determined by chromatin immunoprecipitation. Functional rescue experiments were performed to analyze the impact of ZEB1 and JMJD3 on IL-1ß-induced chondrocytes. RESULTS: IL-1ß treatment induced HMGA1 upregulation, OSI, and inflammatory responses in chondrocytes. HMGA1 downregulation reduced IL-1ß-induced OSI and inflammatory responses in chondrocytes. Mechanically, HMGA1 was bound to the JMJD3 promoter to promote JMJD3 transcription, and JMJD3 induced demethylation of H3K27me3 on the ZEB1 promoter to promote ZEB1 transcription. Overexpression of JMJD3 or ZEB1 neutralized the protective role of silencing HMGA1 in IL-1ß-induced chondrocytes. CONCLUSION: HMGA1 aggravated IL-1ß-induced OSI and inflammatory responses in chondrocytes through the promotion of JMJD3 and ZEB1.

11.
Front Neurosci ; 16: 1058487, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36452330

RESUMO

Recently, attention has been drawn toward brain imaging technology in the medical field, among which MRI plays a vital role in clinical diagnosis and lesion analysis of brain diseases. Different sequences of MR images provide more comprehensive information and help doctors to make accurate clinical diagnoses. However, their costs are particularly high. For many image-to-image synthesis methods in the medical field, supervised learning-based methods require labeled datasets, which are often difficult to obtain. Therefore, we propose an unsupervised learning-based generative adversarial network with adaptive normalization (AN-GAN) for synthesizing T2-weighted MR images from rapidly scanned diffusion-weighted imaging (DWI) MR images. In contrast to the existing methods, deep semantic information is extracted from the high-frequency information of original sequence images, which are then added to the feature map in deconvolution layers as a modality mask vector. This image fusion operation results in better feature maps and guides the training of GANs. Furthermore, to better preserve semantic information against common normalization layers, we introduce AN, a conditional normalization layer that modulates the activations using the fused feature map. Experimental results show that our method of synthesizing T2 images has a better perceptual quality and better detail than the other state-of-the-art methods.

12.
Biomed Environ Sci ; 35(10): 899-910, 2022 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-36443267

RESUMO

Objective: The study aimed to analyze the applicability of the World Health Organization's exclusionary guidelines for Urinary creatinine (Ucr) in the general Chinese population, and to identify Ucr related factors. Methods: We conduct a cross-sectional study using baseline data from 21,167 participants in the China National Human Biomonitoring Program. Mixed linear models and restricted cubic splines (RCS) were used to analyze the associations between explanatory variables and Ucr concentration. Results: The geometric mean and median concentrations of Ucr in the general Chinese population were 0.90 g/L and 1.01 g/L, respectively. And 9.36% samples were outside 0.3-3.0 g/L, including 7.83% below the lower limit and 1.53% above the upper limit. Middle age, male, obesity, smoking, higher frequency of red meat consumption and chronic kidney disease were associated significantly with higher concentrations of Ucr. Results of the RCS showed Ucr was positively and linearly associated with body mass index, inversely and linearly associated with systolic blood pressure, diastolic blood pressure, triglycerides level, and glomerular filtration rate, and were non-linearly associated with triiodothyronine. Conclusion: The age- and gender-specific cut-off values of Ucr that determine the validity of urine samples in the general Chinese population were recommended. To avoid introducing bias into epidemiologic associations, the potential predictors of Ucr observed in the current study should be considered when using Ucr to adjust for variations in urine dilution.


Assuntos
Asiáticos , Pessoa de Meia-Idade , Masculino , Humanos , Creatinina , Estudos Transversais , Taxa de Filtração Glomerular , China
13.
Nanoscale ; 14(46): 17277-17289, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36377319

RESUMO

Silk sericin (SS) is a natural water-soluble protein with the potential to chelate metal ions via its polar groups. However, the difficulty of identifying the saturation of SS limits its application as filter films. One solution is to construct SS filter films with an indicator to reflect the degree of saturation of silk sericin. Hence, the nanocoating consisting of co-assembled SS protein and anthocyanin (C3G) nanoparticles is designed, constructed, and characterized to chelate metal ions with a saturation-visualization detection behavior. Here, metal ions Zn2+ and Al3+ are chosen as models to explore the chelating ability of SS and indicator behaviors of C3G, which could indicate the saturation degree of SS. Interestingly, after the saturation of SS in the solution and filter film situations, the visible color progressively shifts from pink to blue (Zn2+) or violet (Al3+), with the corresponding redshift of UV-Vis absorbance of C3G. Remarkable removal effectiveness of Zn2+ and Al3+, namely 93.16% and 53.97%, as well as an evident saturation-visualization detection, were identified by filter paper films with the nanocoating. Our research provides a fresh viewpoint for designing SS filter films that could effectively remove metal ions while enabling real-time viewing.


Assuntos
Nanopartículas , Sericinas , Seda , Antocianinas , Íons
14.
Mol Neurobiol ; 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36445634

RESUMO

Sepsis is a leading cause of intensive care unit admission and death worldwide. Most surviving patients show acute or chronic mental disorders, which are known as sepsis-associated encephalopathy (SAE). Although accumulating studies in the past two decades focused on the pathogenesis of SAE, a systematic review of retrospective studies which exclusively focuses on the inflammatory mechanisms of SAE has been lacking yet. This review summarizes the recent advance in the field of neuroinflammation and sheds light on the activation of microglia in SAE. Activation of microglia predominates neuroinflammation. As the gene expression profile changes, microglia show heterogeneous characterizations throughout all stages of SAE. Here, we summarize the systemic inflammation following sepsis and also the relationship of microglial diversity and neuroinflammation. Moreover, a collection of neuroinflammation-related dysfunction has also been reviewed to illustrate the possible mechanisms for SAE. In addition, promising pharmacological or non-pharmacological therapeutic strategies, especially those which target neuroinflammation or microglia, are also concluded in the final part of this review. Collectively, clarification of the vital relationship between neuroinflammation and SAE-related mental disorders would significantly improve our understanding of the pathophysiological mechanisms in SAE and therefore provide potential targets for therapies of SAE aimed at inhibiting neuroinflammation.

15.
J Neurooncol ; 160(2): 433-443, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36357822

RESUMO

PURPOSE: Brain radiotherapy combined with concomitant and six cycles of adjuvant temozolomide (TMZ) is the standard treatment for newly diagnosed high-grade gliomas (HGGs). However, the optimal number of cycles of TMZ is still controversial. We conducted this retrospective cohort study to evaluate whether prolonging adjuvant TMZ beyond six cycles resulted in better survival outcomes. METHODS: Patients with high-grade gliomas treated with standard brain radiotherapy combined with TMZ were retrospectively analysed. The duration of adjuvant TMZ ranged from 6 to 12 cycles. Those with 6 cycles of adjuvant TMZ were defined as the standard STUPP group, and those with 7-12 cycles were called the extended STUPP group. Median progression-free survival (PFS) and overall survival (OS) were estimated by the Kaplan-Meier method. The Cox proportional hazards model was adopted to estimate the Hazard ratio (HR) associated with PFS and OS. RESULTS: From September 2011 to May 2021, 372 patients were eligible (143 in the standard group, 229 in the extended group). Patients who received extended STUPP had better PFS and OS compared with standard STUPP. The median PFS for the standard STUPP group was 12 months and for the extended STUPP group 22 months (log-rank P < 0.001). The median OS for the standard STUPP group and extended STUPP group were 12 months and 36 months, respectively (log-rank P < 0.001). In the subgroup analysis, the two treatments did not differ in IDH-mutated patients, while patients with IDH wild-type had a significantly better response to extended treatment than to standard treatment (PFS: log-rank P = 0.004; OS log-rank P = 0.001). Patients with MGMT promoter methylation treated with extended STUPP obtained longer PFS and OS than those treated with standard STUPP (PFS: log-rank P = 0.015; OS log-rank P = 0.010). Adverse events including leukopenia (P < 0.001), thrombocytopenia (P = 0.090), fatigue (P < 0.001) and nausea/vomiting (P = 0.004) were more frequent in the extended group. CONCLUSION: Extended TMZ treatment was superior to standard 6-cycle TMZ for both PFS and OS. The incidence of toxicities in extended group was higher but tolerable.

16.
J Affect Disord ; 323: 10-20, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36403803

RESUMO

BACKGROUND: Major depressive disorder (MDD) is an overbroad and heterogeneous diagnosis with no reliable or quantifiable markers. We aim to combine machine-learning techniques with the individual minimum spanning tree of the morphological brain network (MST-MBN) to determine whether the network properties can provide neuroimaging biomarkers to identify patients with MDD. METHOD: Eight morphometric features of each region of interest (ROI) were extracted from 3D T1 structural images of 106 patients with MDD and 97 healthy controls. Six feature distances of the eight morphometric features were calculated to generate a feature distance matrix, which was defined as low-order MBN. Further linear correlations of feature distances between ROIs were calculated on the basis of low-order MBN to generate individual high-order MBN. The Kruskal's algorithm was used to generate the MST to obtain the core framework of individual low-order and high-order MBN. The regional and global properties of the individual MSTs were defined as the feature. The support vector machine and back-propagation neural network was used to diagnose MDD and assess its severity, respectively. RESULT: The low-order and high-order MST-MBN constructed by cityblock distance had the excellent classification performance. The high-order MST-MBN significantly improved almost 20 % diagnostic accuracy compared with the low-order MST-MBN, and had a maximum R2 value of 0.939 between the predictive and true Hamilton Depression Scale score. The different group-level connectivity strength mainly involves the central executive network and default mode network (no statistical significance after FDR correction). CONCLUSION: We proposed an innovative individual high-order MST-MBN to capture the cortical high-order morphological correlation and make an excellent performance for individualized diagnosis and assessment of MDD.

17.
Front Public Health ; 10: 1005721, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388340

RESUMO

Background: Coal dust is a major risk factor for the occupational health of coal miners, and underground workers with coal mine dust lung disease (Coal miners with coal mine dust lung disease (CMDLD) may have a higher risk of developing Nodular thyroid disease (NTD). The aim of this study was to investigate the relationship between coal mine dust lung disease and the development of Nodular thyroid disease in coal miners. Methods: This was a clinical retrospective observational study that included 955 male coal miners from 31 different coal mining companies in Huainan, Anhui Province, China, who were examined in April 2021 at the Huainan Occupational Disease Prevention and Control Hospital to collect all their clinical physical examination data, including general conditions, laboratory test indices and imaging indices. Based on the presence or absence of Nodular thyroid disease, 429 cases with Nodular thyroid disease were classified as the diseased group and 526 cases without Nodular thyroid disease were classified as the control group. Logistic regression was used to analyse the correlation between the occurrence of Nodular thyroid disease in coal miners, and further single- and multi-factor logistic regression was used to screen the risk exposure factors for Nodular thyroid disease in coal miners. Results: Age, coal mine dust lung disease (CMDLD), red blood cells (RBC), mean red blood cell volume (MCV), albumin (ALB), albumin/globulin (A/G), indirect bilirubin (IBIL), globulin (GLOB), total bilirubin (TBil) and myeloperoxidase (MPO) were associated with the development of Nodular thyroid disease in coal miners (p < 0.05) The results of univariate and multifactorial logistic regression analysis showed that CMDLD (OR:4.5,95%CI:2.79-7.51) had the highest OR and CMDLD was the strongest independent risk exposure factor for the development of Nodular thyroid disease in coal miners. Conclusions: There is a strong correlation between coal mine dust lung disease and Nodular thyroid disease in underground coal miners, and clinicians need to be highly aware of the high risk of NTD in coal miners with CMDLD and adopt individualized clinical prevention strategies.


Assuntos
Doenças Transmissíveis , Pneumopatias , Doenças da Glândula Tireoide , Masculino , Humanos , Poeira , Carvão Mineral , Pneumopatias/epidemiologia , Doenças da Glândula Tireoide/epidemiologia , Mitoxantrona , Bilirrubina , Albuminas
18.
Front Pediatr ; 10: 999684, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36340735

RESUMO

Background: Immunogenic cell death (ICD)-mediated immune response provides a strong rationale to overcome immune evasion in acute lymphoblastic leukemia (ALL). ICD will produce damage-associated molecular patterns (DAMPs) in tumor microenvironment. However, there are few studies on the application of DAMPs-related molecular subtypes in clinically predicting stage III of ALL prognosis. The current study is to identify the DAMPs-associated genes and their molecular subtypes in the stage III of ALL and construct a reliable risk model for prognosis as well as exploring the potential immune-related mechanism. Materials and methods: We used Target and EBI database for differentially expressed genes (DEGs) analysis of the stage III pediatric ALL samples. Three clusters were identified based on a consistent clustering analysis. By using Cox regression and LASSO analysis, we determined DEGs that attribute to survival benefit. In addition, the Gene Set Enrichment Analysis (GSEA) was performed to identify potential molecular pathways regulated by the DAMPs-related gene signatures. ESTIMATE was employed for evaluating the composition of immune cell populations. Results: A sum of 146 DAMPs-associated DEGs in ALL were determined and seven transcripts among them were selected to establish a risk model. The DAMPs-associated gene signature significantly contributed to worse prognosis in the high-risk group. We also found that the high-risk group exhibited low immune cell infiltration and high expression of immune checkpoints. Conclusion: In summary, our study showed that the DAMPs-related DEGs in the stage III of children ALL could be used to predict their prognosis. The risk model of DAMPs we established may be more sensitive to immunotherapy prediction.

19.
RSC Adv ; 12(46): 29928-29938, 2022 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-36321106

RESUMO

Tannic acid (TA) is a natural phenolic compound abundant in plants. Its characteristics of low combustion and good absorption make it useful in the flame retardant field. On this basis, a new expansive flame retardant system (ACT) composed of ammonium polyphosphate (APP)/TA functional clay (CT) was used to study the synergistic flame retardancy and smoke suppression of natural rubber (NR). Because of their unique flame retardancy and better mechanical properties compared with the traditional expansive flame retardant system (IFR), new flame retardants have attracted much attention in various fields. The results of the cone calorimeter showed that the ACT system can significantly influence the decomposition behavior of NR and form a highly graphitized and phosphorous carbon layer to protect the composite material, thus a synergistic effect is produced on the flame retardancy and smoke suppression performance of the composite material. In addition, within the effective additive quality range of the ACT system, TC can give the NR composite excellent mechanical properties.

20.
J Adv Res ; 41: 1-12, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36328739

RESUMO

INTRODUCTION: The human genome encodes two melatonin receptors (MT1 and MT2) that relay melatonin signals to cellular interior. Accumulating evidence has linked melatonin to multiple health benefits, among which its anticancer effects have become well-established. However, the implications of its receptors in lung adenocarcinoma have so far remained incompletely understood. OBJECTIVES: This study aims to investigate the response of the MT1 receptor to melatonin treatment and its dynamic regulation by ubiquitin-specific protease 8 (USP8) in lung adenocarcinoma. METHODS: The mRNA levels of MT1 and MT2 receptors were analyzed with sequencing data. The expression and localization of the MT1 receptor with melatonin treatment were investigated by immunoblotting, immunofluorescence and confocal microscopy assays. Endocytic deubiquitylases were screened to identify MT1 association. The effects of USP8 were assessed with shRNA-mediated knockdown and small molecule inhibitor. The combined efficacy of melatonin and USP8 suppression was also evaluated using xenograft animal models. RESULTS: Bioinformatic analysis revealed increased expression of the MT1 receptor in lung adenocarcinoma tissues. Melatonin treatment leads to the downregulation of the MT1 receptor in lung adenocarcinoma cells, which is attributed to receptor endocytosis and lysosomal degradation via the canonical endo-lysosomal route. USP8 negatively regulates the endocytic degradation of the MT1 receptor incurred by melatonin exposure and thus protects lung adenocarcinoma cell growth. USP8 suppression by knockdown or pharmacological inhibition effectively deters cancer cell proliferation and sensitizes lung adenocarcinoma cells to melatonin in vitro. Furthermore, USP8 silencing significantly potentiates the anticancer effects of melatonin in xenograft tumor models. CONCLUSION: The MT1 receptor responds to melatonin treatment and is endocytosed for lysosomal degradation that is counteracted by USP8. The inhibition of USP8 demonstrates tumor-suppressive effects and thus can be exploited as potential therapeutic strategy either as monotherapy or combined therapy with melatonin.


Assuntos
Adenocarcinoma de Pulmão , Adenocarcinoma , Neoplasias Pulmonares , Melatonina , Animais , Humanos , Receptor MT1 de Melatonina/genética , Receptor MT1 de Melatonina/metabolismo , Receptor MT2 de Melatonina/metabolismo , Melatonina/farmacologia , Adenocarcinoma de Pulmão/tratamento farmacológico , Adenocarcinoma/tratamento farmacológico , Neoplasias Pulmonares/tratamento farmacológico , Proteases Específicas de Ubiquitina
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...