Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.812
Filtrar
1.
Eur Radiol ; 30(1): 413-424, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31332558

RESUMO

BACKGROUND: We attempted to train and validate a model of deep learning for the preoperative prediction of the response of patients with intermediate-stage hepatocellular carcinoma (HCC) undergoing transarterial chemoembolization (TACE). METHOD: All computed tomography (CT) images were acquired for 562 patients from the Nan Fang Hospital (NFH), 89 patients from Zhu Hai Hospital Affiliated with Jinan University (ZHHAJU), and 138 patients from the Sun Yat-sen University Cancer Center (SYUCC). We built a predictive model from the outputs using the transfer learning techniques of a residual convolutional neural network (ResNet50). The prediction accuracy for each patch was revaluated in two independent validation cohorts. RESULTS: In the training set (NFH), the deep learning model had an accuracy of 84.3% and areas under curves (AUCs) of 0.97, 0.96, 0.95, and 0.96 for complete response (CR), partial response (PR), stable disease (SD), and progressive disease (PD), respectively. In the other two validation sets (ZHHAJU and SYUCC), the deep learning model had accuracies of 85.1% and 82.8% for CR, PR, SD, and PD. The ResNet50 model also had high AUCs for predicting the objective response of TACE therapy in patches and patients of three cohorts. Decision curve analysis (DCA) showed that the ResNet50 model had a high net benefit in the two validation cohorts. CONCLUSION: The deep learning model presented a good performance for predicting the response of TACE therapy and could help clinicians in better screening patients with HCC who can benefit from the interventional treatment. KEY POINTS: • Therapy response of TACE can be predicted by a deep learning model based on CT images. • The probability value from a trained or validation deep learning model showed significant correlation with different therapy responses. • Further improvement is necessary before clinical utilization.

2.
Toxicol Lett ; 318: 104-113, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31672611

RESUMO

BACKGROUND: Bupivacaine (BP) is commonly used as a local anaesthetic(LA) in the clinic, but it can also cause neurotoxicity, especially in patients with diabetes. Previous studies have found that high-glucose environments can aggravate BP-induced DNA damage in nerve cells. Ku70 is subunit of the DNA damage repair enzyme DNA-PK. This study was designed to determine whether high-glucose conditions enhance BP neurotoxicity and DNA damage by inhibiting Ku70 expression. METHODS: We examined the effect of BP on apoptosis and DNA damage in murine dorsal root ganglion (DRG) neurons under hyperglycaemic conditions. Untreated DRG cells and DRG cells pretreated with NU7441, a DNA-PK inhibitor, were cultured for 3 days under normal culture conditions or with 50 mM glucose, and the cells were then treated with BP for 3 h. DNA damage was investigated via comet assays, the ratio of early to late apoptotic cells was assessed by Annexin V-FITC/PI staining, and cell viability was measured by CCK-8 assays. The protein expression levels of DNA-PK, Ku70, Bax, Bcl-2 and γH2ax were measured by immunofluorescence or Western blotting. RESULTS: Compared to its effect under normal culture conditions, BP treatment led to decreased cell viability and increased DNA damage in DRG cells grown under high-glucose conditions. The rate of DRG cell apoptosis and the expression of γH2ax, the ratio of Bax to Bcl-2 also increased under the high-glucose conditions. Furthermore, Ku70 expression was inhibited. The DNA-PK inhibitor, NU7441, could significantly inhibit DNA-PK and Ku70 expression, simultaneously further aggravating BP-induced apoptosis and DNA damage under high-glucose conditions. CONCLUSION: These data indicate that hyperglycaemia may enhance BP-induced neurotoxicity and DNA damage by inhibiting the DNA repair protein Ku70.


Assuntos
Anestésicos Locais/toxicidade , Apoptose/efeitos dos fármacos , Bupivacaína/toxicidade , Cromonas/toxicidade , Inibidores Enzimáticos/toxicidade , Gânglios Espinais/efeitos dos fármacos , Glucose/toxicidade , Autoantígeno Ku/antagonistas & inibidores , Morfolinas/toxicidade , Síndromes Neurotóxicas/etiologia , Animais , Células Cultivadas , Dano ao DNA , Gânglios Espinais/enzimologia , Gânglios Espinais/patologia , Autoantígeno Ku/metabolismo , Camundongos , Síndromes Neurotóxicas/enzimologia , Síndromes Neurotóxicas/patologia , Transdução de Sinais/efeitos dos fármacos
3.
Pac Symp Biocomput ; 25: 139-148, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31797593

RESUMO

Computed tomographic (CT) is a fundamental imaging modality to generate cross-sectional views of internal anatomy in a living subject or interrogate material composition of an object, and it has been routinely used in clinical applications and nondestructive testing. In a standard CT image, pixels having the same Hounsfield Units (HU) can correspond to different materials, and it is therefore challenging to differentiate and quantify materials. Dual-energy CT (DECT) is desirable to differentiate multiple materials, but the costly DECT scanners are not widely available as single-energy CT (SECT) scanners. Recent advancement in deep learning provides an enabling tool to map images between different modalities with incorporated prior knowledge. Here we develop a deep learning approach to perform DECT imaging by using the standard SECT data. The end point of the approach is a model capable of providing the high-energy CT image for a given input low-energy CT image. The feasibility of the deep learning-based DECT imaging method using a SECT data is demonstrated using contrast-enhanced DECT images and evaluated using clinical relevant indexes. This work opens new opportunities for numerous DECT clinical applications with a standard SECT data and may enable significantly simplified hardware design, scanning dose and image cost reduction for future DECT systems.

4.
Cytokine ; 125: 154850, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31557635

RESUMO

BACKGROUND: Endometrial injury can result in thin endometrium and subfertility. Granulocyte macrophage colony stimulating factor (GM-CSF) contributes to tissue repair, but its role in endometrial regeneration has not been investigated. METHODS: To determine the effect of GM-CSF on endometrial regeneration, we established a mouse model of thin endometrium by uterine perfusion with 20 µL 90% ethanol. Thin endometrium in mice was featured by lowered endometrial thickness, decreased expression of Ki67 in glandular cells, and a reduced number of implantation sites. To explore the mechanism of GM-CSF on endometrial regeneration, endometrium was obtained from patients undergoing hysterectomy or hysteroscopy and endometrial biopsy. Effects of GM-CSF on primary cultured human endometrial glandular and stromal cells were examined by the 5-bromo-2'-deoxyuridine (BrdU) proliferation assay and transwell migration assay, followed by exploration of the potential signaling pathway. RESULTS: GM-CSF intraperitoneal (i.p.) injection significantly increased endometrial thickness, expression of Ki67 in endometrial glandular cells, and the number of implantation sites. GM-CSF significantly promoted proliferation of primary human endometrial glandular cells and migration of stromal cells. GM-CSF activated p-Akt and increased expressions of p70S6K and c-Jun, which were blocked by LY294002. CONCLUSION: We found that GM-CSF could improve endometrial regeneration, possibly through activating PI3K/Akt signaling pathway.

5.
Toxicol In Vitro ; 62: 104668, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31629073

RESUMO

Methamphetamine (MA) has a high uptake in lung, but the precise mechanism of MA-induced lung toxicity remains unclear. The aim of this study is to investigate the role of MA abuse in remodeling of pulmonary arteries and to explore the possible correlation of the association of the remodeling with the redox imbalance in pulmonary arterial smooth muscle cells (PASMCs). Wistar rats were randomly divided into control group and MA group for the experimental study. We employed H&E staining, western blot, immunofluorescence, knockdown, flow in our experimental approach. Our studies shows that chronic exposure to MA led to weight loss, increased pulmonary arterial pressure, hypertrophy of right ventricle and remodeling of pulmonary arterial wall of rats. Our cell culture study with PASMCs indicates that MA significantly induced the imbalance between proliferation and apoptosis by upregulating the level of PCNA, Bcl-2 and reduction in the expression of BAX and Caspase 3. MA markedly prevented the nuclear translocation of Nrf2 to inhibit antioxidation. The knockdown of Nrf2 expression using siRNA significantly elevated the expression of SOD2/GCS and the production of ROS in PASMCs and even scaled up the amount of PASMCs induced by MA. Linear regression analysis showed that knockdown of Nrf2 promoted the positive correlation of relative ROS level with proliferation of PASMCs. Therefore, chronic exposure to MA induces pulmonary arterial remodeling by Nrf2-mediated imbalance of redox system to aggravate oxidative stress, and Nrf2 is a possible target for the treatment of MA-lung toxicity.

6.
Biosens Bioelectron ; 148: 111836, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31731074

RESUMO

This work reports a customized methodology for the fabrication of 3D CdS nanosheet (NS)-enwrapped carbon fiber framework (CFF) and its utilization for sensitive split-type CuO-mediated PEC immunoassay. Specifically, the 3D CdS NS-CFF was fabricated via a solvothermal process, while the sandwich immunocomplexing was allowed in a 96 well plate with CuO nanoparticles (NPs) as the signaling labels. The subsequent release of the Cu2+ ions was directed to interact with the CdS NS, generating trapping sites and thus inhibiting its photocurrent generation. In such a protocol, the 3D CdS NS-CFF photoelectrode could not only guarantee its sufficient contact with the Cu2+-containing solution but also supply plenty CdS surface for the Cu2+ ions. Because of the target-dependent release of the Cu2+ ions and its proper coupling with the 3D CdS NS-CFF photoelectrode, a sensitive split-type PEC immunoassay was achieved for the detection of brain natriuretic peptide (BNP). This proposed system exhibited good stability and selectivity, and its applicability for real sample analysis was also demonstrated via comparison with the commercial BNP enzyme-linked immunosorbent assay (ELISA) kit. We expect this work could stimulate more interest in the design and utilization of 3D photoelectrodes for novel PEC bioanalysis.

7.
Methods Mol Biol ; 2077: 141-163, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31707657

RESUMO

Two-component systems allow bacteria to respond to changes in environmental or cytosolic conditions through autophosphorylation of a histidine kinase (HK) and subsequent transfer of the phosphate group to its downstream cognate response regulator (RR). The RR then elicits a cellular response, commonly through regulation of transcription. Engineering two-component system signaling networks provides a strategy to study bacterial signaling mechanisms related to bacterial cell survival, symbiosis, and virulence, and to develop sensory devices in synthetic biology. Here we focus on the principles for engineering the HK to identify unknown signal inputs, test signal transmission mechanisms, design small molecule sensors, and rewire two-component signaling networks.

8.
Acta Trop ; 201: 105211, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31600522

RESUMO

Enterocytozoon bieneusi is a potentially important zoonotic pathogen. However, there is no information on E. bieneusi infection of captive long-tailed macaques (Macaca fascicularis) in Hainan Province, China. Here 193 fecal specimens of M. fascicularis were collected from a breeding base in Hainan Province, China, housing non-human primates for experimental use. E. bieneusi was identified and genotyped by nested PCR analysis of the internal transcribed spacer (ITS) region of the rRNA gene. A total of 59 (30.6%) specimens were PCR-positive for E. bieneusi and 16 ITS genotypes were identified including nine known genotypes: Type IV (n = 19), D (n = 11), CM1 (n = 8), PigEBITS7 (n = 4), Pongo2 (n = 4), Peru8 (n = 3), Peru11 (n = 1), WL21 (n = 1) and CM2 (n = 1) and seven novel genotypes HNM-I to HNM-VII (one each). Importantly, genotypes D, Type IV, Peru8, PigEBITS7, and Peru11, which were the predominant (38/59, 64.4%) genotypes identified among captive M. fascicularis in this study, are also well-known human-pathogenic genotypes. All the genotypes of E. bieneusi identified here, including the seven novel ones, belonged to zoonotic Group 1. This is the first report of the identification of E. bieneusi in M. fascicularis in Hainan Province, China. The finding that the numerous known human-pathogenic types and seven novel genotypes of E. bieneusi all belong to zoonotic Group 1 indicates the possibility of transmission of this important pathogenic parasite between M. fascicularis and humans.

9.
J Nanosci Nanotechnol ; 20(4): 2308-2315, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492241

RESUMO

While most of the fluorescent nanoparticles used in stimulated emission depletion (STED) microscopy have a long excitation wavelength, many applications need shorter wavelength fluorophores, which are yet to be developed for STED microscopy applications. Here, three kinds of fluorescent nanoparticles, namely silica nanoparticles (NFv465), fluoro-max blue aqueous fluorescent nanoparticles (FBs) and light yellow nanoparticles (LYs) with short excitation wavelength in violet band have been studied to assess whether they are applicable in STED microscopy. The experimental configuration utilizes a 405 nm continuous wave (CW) laser as excitation beam and a 532 nm CW laser as depletion beam. We compare the photostability, photobleaching and depletion efficiency of three kinds of fluorescent nanoparticles in a series of experiments. Light yellow nanoparticles are proved to be a good candidate as fluorophore in STED microscopy.

10.
Carbohydr Polym ; 228: 115410, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31635751

RESUMO

A polysaccharide (PPS) in peanut sediment of aqueous extraction process was obtained at pH4.0, purified via anion-exchange chromatography. The composition, chain conformation and rheological properties were investigated. PPS mainly consisted of arabinose, galacturonic acid, xylose, and rhamnose. The intrinsic viscosity [η] was 0.71 dL/g in 0.1 M NaNO3 solution. The weight-average molar mass Mw and polydispersity index were 3.77 × 105 g/mol and 1.25, suggesting high homogeneity. The average radius of gyration (Rg), hydrodynamic radius (Rh), Rg/Rh ratio and conformation parameter v were 25.5, 18.2, 1.40 and 0.21, respectively, indicating compact coil chain conformation with branched structure. Molecular morphology revealed that PPS displayed chain shape comprised of spheres with a diameter range of 15-50 nm and apparent length of chains mainly ranged from 100 to 300 nm. The aggregation caused by molecular self-association enhanced with concentration increasing. Additionally, Newtonian behavior was observed at various concentrations. Increase in temperature effectively broke this behavior. 10.0 wt.% PPS possessed activation energy of 21.7 KJ/mol, was structured liquid and almost fitted Cox-Merz rule. These closely related with its conformation and molecular self-association behavior.

11.
Brain Lang ; 201: 104714, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31790907

RESUMO

The framework of embodied cognition has challenged the modular view of a language-cognition divide by suggesting that meaning-retrieval critically involves the sensory-motor system. Despite extensive research into the neural mechanisms underlying language-motor coupling, it remains unclear how the motor system might be differentially engaged by different levels of linguistic abstraction and language proficiency. To address this issue, we used fMRI to quantify neural activations in brain regions underlying motor and language processing in Chinese-English speakers' processing of literal, metaphorical, and abstract language in their L1 and L2. Results overall revealed a response in motor ROIs gradually attenuating in intensity from literal to abstract via metaphorical language in both L1 and L2. Furthermore, contrast analyses between L1 and L2 showed overall greater activations of motor ROIs in the L2. We conclude that motor involvement in language processing is graded rather than all-or-none and that the motor system has a dual-functional role.

12.
Sci Rep ; 9(1): 18155, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31796759

RESUMO

By manipulating the reference pulses amplitude, a security vulnerability is caused by self-reference continuous-variable quantum key distribution. In this paper, we formalize an attack strategy for reference pulses, showing that the proposed attack can compromise the practical security of CVQKD protocol. In this scheme, before the beam splitter attack, Eve intercepts the reference pulses emitted by Alice, using Bayesian algorithm to estimate phase shifts. Subsequently, other reference pulses are re-prepared and resubmitted to Bob. In simulations, Bayesian algorithm effectively estimates the phase drifts and has the high robustness to noise. Therefore, the eavesdropper can bias the excess noise due to the intercept-resend attack and the beam splitter attack. And Alice and Bob believe that their excess noise is below the null key threshold and can still share a secret key. Consequently, the proposed attack shows that its practical security can be compromised by transmitting the reference pulses in the continuous-variable quantum key distribution protocol.

13.
Plant Biotechnol J ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31799788

RESUMO

Coriander (Coriandrum sativum L. 2n = 2x = 22), a plant from the Apiaceae family, also called cilantro or Chinese parsley, is a globally important crop used as vegetable, spice, fragrance, and traditional medicine. Here, we report a high-quality assembly and analysis of its genome sequence, anchored to 11 chromosomes, with total length of 2,118.68 Mb and N50 scaffold length 160.99 Mb. We found that two whole-genome duplication events, respectively dated to ~ 45-52 and ~ 54-61 million years ago, were shared by the Apiaceae family after their split from lettuce. Unbalanced gene loss and expression observed between duplicated copies produced by these two events. Gene retention, expression, metabolomics and comparative genomic analyses of Terpene synthase (TPS) gene family, involved in terpenoid biosynthesis pathway contributing to coriander's special flavor, revealed that tandem duplication contributed to coriander TPS gene family expansion, especially compared to their carrot counterparts. Notably, a TPS gene highly expressed in all 4 tissues and 3 development stages studied, is likely a major-effect gene encoding linalool synthase and myrcene synthase. The present genome sequencing, transcriptome, metabolome and comparative genomic efforts provide valuable insights into the genome evolution and spice trait biology of Apiaceae and others related plants, and facilitated further research into important gene functions and crop improvement.

14.
Head Neck ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31785054

RESUMO

BACKGROUND: No study has determined the incidence of long-term opioid use, or risk factors for long-term use, ≥1 year after radiotherapy. METHODS: Medical records of 276 head/neck cancer patients were retrospectively assessed for persistent opioid use 1-year after curative-intent radiotherapy. Numerous potential risk factors were assessed and the physicians' documented reasons for continued use were qualitatively categorized as suspected opioid use disorder (OUD) or as medically indicated for control of ongoing pain. RESULTS: Of note, 20 of 276 patients continued using opioids long-term. High maximum opioid dose and the use of opioids and/or psychotropics/non-opioid analgesics at the radiation oncology intake visit were associated with this outcome. Three patients continued due to suspected OUD and 17 due to medical indications. CONCLUSION: Of note, 7.2% of patients developed long-term opioid use, which was associated with high maximum opioid dose and early initiation of opioids and/or psychotropics/non-opioid analgesics. Physicians cited medical indications as the primary reason for continued use.

15.
Aging (Albany NY) ; 112019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801111

RESUMO

BACKGROUND: Tumor recurrence is the main cause of poor prognosis of GBM. Finding the characteristics of recurrent GBM that provide early warning of tumor recurrence can provide guidance for the clinical treatment of recurrent GBM. RESULTS: Reactive oxygen species (ROS) biosynthetic processes was significantly elevated in recurrent GBM. The recurrent risk score based on the ROS biosynthetic process was closely related to tumor purity and tumor immune functions. The quantitative risk assessment system could be used to predict the recurrence time of GBM. Gallic acid, a compound with high anti-oxidation activity and low cytotoxicity, was screened as a potential chemotherapy sensitizer for recurrent GBM. CONCLUSION: The quantitative risk assessment system based on ROS biosynthetic process could be used for early warning of GBM recurrence. Combination of low-dose gallic acid and temozolomide could improve therapeutic outcomes in recurrent GBM. METHODS: A total of 663 primary and recurrent GBM samples with clinical and microarray data were included in this study. GSVA, LASSO-COX, and Kaplan-Meier survive curve were performed to construct and verify a quantitative risk assessment system for GBM recurrence prediction. An antioxidant capacity test and cell viability test were used to discover potential drugs for recurrent GBM.

16.
World J Surg Oncol ; 17(1): 206, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801560

RESUMO

BACKGROUND: Malignant transformation of endometriosis in the rectovaginal septum is rare and usually misdiagnosed as a colorectal or gynecological tumor. We report a rare case of primary endometrioid adenocarcinoma of the rectovaginal septum with invasion of the rectum. CASE PRESENTATION: A 57-year-old overweight woman presented with vaginal bleeding and self-reported left lower abdominal pain during the previous 2 weeks. Preoperative imaging showed a large pelvic mass with invasion of the rectum, suggestive of a gynecologic malignancy. Multiple endoscopic biopsies and immunohistochemical analyses of specimens was performed. The patient received joint gynecological-surgical laparotomy, and there were no intra- or postoperative complications. The histopathological diagnosis was rectovaginal endometrioid adenocarcinoma with rectum infiltration. The patient received adjuvant chemotherapy and achieved good treatment response, with no early complications. At 12 months after surgery, there was no evidence of recurrence. CONCLUSIONS: A high index of clinical suspicion is required for the diagnosis of endometrioid adenocarcinoma in the rectovaginal septum. Surgery combined with additional chemotherapy or radiotherapy seems to be a standard treatment, and hormonal therapy is optional. The efficacies of other therapies, including targeted medication and immunotherapy, are unknown.

17.
J Mech Behav Biomed Mater ; 103: 103543, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31783284

RESUMO

The study of the gating mechanism of mechanosensitive channels opens a window to the exploration of how different mechanical stimuli induce adaptive cellular behaviors of both the protein and the lipid, across different time and length scales. In this work, through a molecular dynamics-decorated finite element method (MDeFEM), the gating behavior of mechanosensitive channels of small conductance (MscS) in Escherichia coli (E. coli) is studied upon membrane stretch or global bending. The local membrane curvature around MscS is incorporated, as well as multiple MscL (mechanosensitive channels of large conductance) molecules in proximity to MscS. The local membrane curvature is found to delay MscS opening and diminishes moderately upon membrane stretching. Mimicking the insertion of lysophosphatidylcholine (LPC) molecules into the lipid, both downward and upward bending can active MscS, as long as the global membrane curvature radius reaches 34 nm. Based on the different MscS pore evolutions observed with the presence of one or more MscLs nearby, we propose that when coreconstituted, multiple MscL molecules tend to be located at the local membrane curvature zone around MscS. In another word, as MscL "swims around" in the lipid bilayer, it can be trapped by the membrane's local curvature. Collectively, the current study provides valuable insights into the interplay between mechanosensitive channels and lipid membrane at structural and physical levels, and specific predictions are proposed for further experimental investigations.

18.
Horm Metab Res ; 51(11): 729-734, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31683343

RESUMO

Contrasting data about the association between proliferative diabetic retinopathy (PDR) and vitamin D status remain unknown. First, a hospital-based cross-sectional study consisting of 889 diabetic retinopathy (DR) and non-DR (NDR) patients was admitted. Further the accumulated evidence was performed to explore the association and dose-response relationship. Our study indicated that the odd ratio for PDR in vitamin D deficiency (VDD) individuals was significantly increased (1.60, 95% CI 1.06-2.42), compared with NDR in vitamin D sufficiency individuals, adjusted by age, sex, diabetic duration, and HbA1c. Four studies plus our study with data on vitamin D levels in 4970 patients with PDR and NDR subjects are compared. Association between vitamin D deficiency and risk of PDR exists (OR=1.69, 95% CI 1.40-2.05; I2=0%, p=0.61). Association between a nonlinear trend for vitamin D decrease with risk of DR was significant (chi2=16.53, p=0.0003). No significant heterogeneity in identified studies was found (goodness of fit chi2=2.98, p=0.225). It is concluded that vitamin D deficiency is significantly associated with risk of proliferative diabetic retinopathy.

19.
Chemosphere ; 241: 125133, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31683427

RESUMO

Black carbon (BC) plays a vital role in atmospheric environment and climate change. Temporal variations and transport pathways of BC in Xiamen, China with the impacts of synoptic circulation were investigated in 2014 with Aethalometer. Annual mean BC concentration was 4270 ng m-3. BC exhibited clear diurnal (seasonal) variations, with the maximum of 6182 (4755) ng m-3 at 6:00 (in spring) and minimum of 2847 (3774) ng m-3 at 13:00 (in summer). Conditional probability function analysis indicated that high BC concentrations were associated with northwesterly winds with low wind speed. Air masses originating from the East China Sea and passing along with East China Coast had the highest BC concentrations. Potential source contribution function and concentration weighted trajectory analysis suggested that major sources for BC included the surrounding region, southwestern Fujian and eastern Guangdong to the southwest, Hubei, Hunan and Jiangxi to the northwest, the East China Sea and the South China Sea. Of the nine synoptic circulation patterns, three cyclone-related patterns were associated with low BC concentrations and small biomass burning (BCbb) contributions. Of the six anticyclone-related patterns, the three cold-high circulations around winter were associated with moderate BC concentrations and large BCbb contributions. The two cold-high patterns in spring and autumn were associated with high BC concentrations and small BCbb contributions, while the warm-high pattern was associated with moderate BC concentration and small BCbb contribution. The findings provide insights into the transport mechanisms of BC with the impacts of synoptic pattern in China.

20.
Urology ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31711982

RESUMO

OBJECTIVE: To study if prostatic ductal adenocarcinoma (PDA) controlled by Grade Group (GG), PSA, and tumor volume (TV) is an independent predictor of adverse radical prostatectomy (RP) outcomes. MATERIALS: One-hundred-and-twenty-eight PDA and 1,141 acinar continuous RPs were studied. Each tumor nodule (TN) was individually graded, staged, and its TV measured. Univariate analysis (UVA) identified features associated with lymph node metastasis (LN+), extraprostatic extension (EPE), positive surgical margins (SM+), and seminal vesicle invasion (SV+). We then assessed PDA effect on RP outcomes in a multivariate analysis (MVA). RESULTS: In 127 cases PDA was present in 1 TN and no TN was pure PDA. One-hundred-and-twenty-three cases had PDA in TNs with highest grade, stage, and TV. Patients with PDA were older (65 vs. 63 years, p<0.001), had higher GG (p<0.001), and LN+ (6.3% vs 2.7%, p=0.049). Controlling these variables by GG eliminated statistical significance. Overall, there were 3,249 separate TNs (129 PDA and 3,120 acinar). In UVA, PDA predicted EPE (92/124 vs 517/3,045), SV+ (28/1129 vs 116/3,120), and SM+ (51/129 vs 296/3,120), all p<0.001. In MVA, PDA lost its effect on EPE (OR=0.88, p=0.64), SM+ (OR=0.86, p=0.5), and SV+ (OR=0.99, p=0.98). CONCLUSION: Controlled for grade and TV, PDA was not an independent predictor of adverse RP outcomes, but former two were. Hence, higher GG and TV associated with PDA TNs may be predictive of adverse RP outcomes rather than PDA by itself. These conclusions may be used in preoperative risk stratification and definitive therapy planning when PDA is identified on needle biopsy.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA