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1.
Sci Total Environ ; 951: 175444, 2024 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-39134276

RESUMO

Underground pipelines serve as critical infrastructure for gas transmission, strategically buried for safety, environmental, and economic considerations. Despite their importance, operational challenges and external interferences can lead to underground gas leaks with potentially catastrophic consequences for both human safety and the environment. The presence of a protective soil bed introduces complexities in understanding subsurface transport phenomena and quantifying gas releases accurately. Herein, this review presents a systematic analysis of published research in the field of underground gas releases, with an emphasis on interdisciplinary approaches that connect the lithosphere and atmosphere. The analysis highlights the broad spectrum of employed methods, including theoretical models based on fundamental principles, empirical formulations derived from experimental data, and sophisticated computational tools. A clear fundamental understanding and computational analysis, and to a lesser extent experimental, have been established to describe the migration regime. In contrast, more empirical research has addressed the crater formation regime, though focus was given to the far-field modelling following the soil ejection rather than the transient phenomena leading to the formation of the crater. Additionally, this review touches upon practical and conceptual topics, such as detection and localization techniques, and flow regimes in other gaseous flows through soil and powder beds, putting into question the applicability of some presumed granulated concepts to the flowing behavior expected beyond migration. The research landscape predominantly focuses on investigating the influence of release parameters on the release phenomena only from the atmospheric or soil domain perspective. This work provides insights that aim to first transcend both domains and then bridge the three distinct flow regimes-migration, uplift, and crater formation-despite the limited acknowledgment of the necessity of addressing all regimes concurrently through a universal approach. This review serves as a valuable resource for engineers to develop innovative solutions for the management of risks associated with underground gas leaks.

2.
Acta Biomater ; 175: 157-169, 2024 02.
Artigo em Inglês | MEDLINE | ID: mdl-38159896

RESUMO

The meniscal tissue is a layered material with varying properties influenced by collagen content and arrangement. Understanding the relationship between structure and properties is crucial for disease management, treatment development, and biomaterial design. The internal layer of the meniscus is softer and more deformable than the outer layers, thanks to interconnected collagen channels that guide fluid flow. To investigate these relationships, we propose an integrated approach that combines Computational Fluid Dynamics (CFD) with Image Analysis (CFD-IA). We analyze fluid flow in the internal architecture of the human meniscus across a range of inlet velocities (0.1 mm/s to 1.6 m/s) using high-resolution 3D micro-computed tomography scans. Statistical correlations are observed between architectural parameters (tortuosity, connectivity, porosity, pore size) and fluid flow parameters (Re number distribution, permeability). Some channels exhibit Re values of 1400 at an inlet velocity of 1.6 m/s, and a transition from Darcy's regime to a non-Darcian regime occurs around an inlet velocity of 0.02 m/s. Location-dependent permeability ranges from 20-32 Darcy. Regression modelling reveals a strong correlation between fluid velocity and tortuosity at high inlet velocities, as well as with channel diameter at low inlet velocities. At higher inlet velocities, flow paths deviate more from the preferential direction, resulting in a decrease in the concentration parameter by an average of 0.4. This research provides valuable insights into the fluid flow behaviour within the meniscus and its structural influences. 3D models and image stack are available to download at https://doi.org/10.5281/zenodo.10401592. STATEMENT OF SIGNIFICANCE: The meniscus is a highly porous soft tissue with remarkable properties of load transfer and energy absorption. We give insight on the mechanism of energy absorption from high resolution uCT scans, never presented before, and a new method which combine CFD and image. The structure is similar to a sandwich structure with a stiff outside layer and a soft internal layer made of collagen channels oriented in a preferential direction guiding the fluid flow, enabling it to accommodate deformation and dissipate energy, making it a potentially optimized damping system. We investigate architectural/ fluid flow parameters- fluid regimes relationship, which is of interest of the readers working on designing suitable biomimetic systems that can be adopted for replacement.


Assuntos
Hidrodinâmica , Menisco , Humanos , Simulação por Computador , Microtomografia por Raio-X , Menisco/diagnóstico por imagem , Colágeno
3.
Sci Total Environ ; 867: 161499, 2023 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-36634769

RESUMO

Dam construction is the main factor altering the flow regimes, while few studies have described that in small and medium-sized rivers (SMRs). The universal indicators of hydrological alteration (IHA) that are widely used in large rivers calculate the parameters just on the annual scale and omit the intra-annual seasonal differences of the flow regimes in SMRs. To fully quantify dam-induced impacts on the flow regimes in SMRs, this paper proposes the improved IHA (IIHA) based on the universal IHA. Then two methods of range of variable approach (RVA) and histogram matching approach (HMA) are used to assess the flow regime alteration. Finally, two indicators of water quantity level (WQL)/hydrological alteration (HA) defined by the parameters in IIHA are employed to evaluate the influence of flow regime alteration on the riverine ecosystem. The case study of a typical SMR named Liujiaping River in Hunan Province, China, verifies the necessity of improving IHA where more hydrological parameters calculated in different periods can comprehensively reflect the flow regime alteration. We find that the integrated hydrological alteration of Liujiaping River assessed by RVA and HMA are both at a high level, and the flow regimes have been significantly altered after the dam construction. Also, the indicators of WQL and HA have higher correlation coefficients with 77 of IIHA parameters and thus can retain as much information of the flow regimes as possible to evaluate the influence of its alteration on the riverine ecosystem.

4.
Sensors (Basel) ; 22(7)2022 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-35408432

RESUMO

The present paper compares, for the first time, the regimes of a pulsating turbulent flow in a smooth pipe in terms of 0.001 ≤ ω+ ≤ 0.0346 and 0.16 ≤ ß ≤ 0.63 at Re ≈ 7000 with the uncertainty in estimating the flow rate by an ultrasonic flowmeter. It was revealed that the classification of pulsating flow regimes according to the dimensionless angular frequency ω+ does not have a direct relation with the K parameter equal to the ratio of the phase-average calibration constant in pulsating flow to the corresponding value in steady flow. The results of data processing showed that K depends on the relative amplitude of pulsations ß and the position of the chord of the ultrasonic beam trajectory (L/R is distance L from the pipe center to the chord to the pipe radius R). In the coordinates ß and L/R, there is a rather wide area where the uncertainty in flow rate estimation of pulsating flows should not exceed 0.5%. An increase in ß or L/R leads to an increase in measurement uncertainty, which in the limiting case ß, L/R → 1 can reach 5% or more. Favorable and unfavorable areas of the pipe section were identified when scanning pulsating flows and the effectiveness of using multi-path scanning schemes was estimated to reduce the resulting effect of flow pulsations on flow measurement uncertainty.

5.
Micromachines (Basel) ; 13(2)2022 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-35208284

RESUMO

The T-shaped micro-junction is among the most used geometry in microfluidic applications, and many design modifications of the channel walls have been proposed to enhance mixing. In this work, we investigate through numerical simulations the introduction of one pair of small rectangular cavities in the lateral walls of the mixing channel just downstream of the confluence region. The aim is to preserve the simple geometry that has contributed to spread the practical use of the T-shaped micro-junction while suggesting a modification that should, in principle, work jointly with the vortical structures present in the mixing channel, further enhancing their efficiency in mixing without significant additional pressure drops. The performance is analyzed in the different flow regimes occurring by increasing the Reynolds number. The cavities are effective in the two highly-mixed flow regimes, viz., the steady engulfment and the periodic asymmetric regimes. This presence does not interfere with the formation of the vortical structures that promote mixing by convection in these two regimes, but it further enhances the mixing of the inlet streams in the near-wall region of the mixing channel without any additional cost, leading to better performance than the classical configuration.

6.
Proc Natl Acad Sci U S A ; 119(8)2022 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-35165178

RESUMO

Mean annual temperature and mean annual precipitation drive much of the variation in productivity across Earth's terrestrial ecosystems but do not explain variation in gross primary productivity (GPP) or ecosystem respiration (ER) in flowing waters. We document substantial variation in the magnitude and seasonality of GPP and ER across 222 US rivers. In contrast to their terrestrial counterparts, most river ecosystems respire far more carbon than they fix and have less pronounced and consistent seasonality in their metabolic rates. We find that variation in annual solar energy inputs and stability of flows are the primary drivers of GPP and ER across rivers. A classification schema based on these drivers advances river science and informs management.


Assuntos
Ecossistema , Rios , Carbono/metabolismo , Luz , Estações do Ano , Temperatura , Tempo (Meteorologia)
7.
Rev. biol. trop ; 69(2)jun. 2021.
Artigo em Espanhol | LILACS, SaludCR | ID: biblio-1387642

RESUMO

Resumen Introducción: A pesar de su importancia en el rendimiento de la natación y la eficacia biológica individual, la forma del cuerpo y las variaciones de tamaño en respuesta a la velocidad del flujo de agua siguen siendo desconocidas para la mayoría de los peces migratorios de agua dulce en el Neotrópico. Objetivo: Contrastar la hipótesis de variación fenotípica en el tamaño y la conformación del cuerpo de dos especies de peces dulceacuícolas que enfrentan cambios de velocidad a través de sus migraciones en dos ríos pertenecientes a una cuenca neotropical. Métodos: Se utilizó la morfometría geométrica basada en hitos para estudiar las variaciones en el tamaño y la conformación del cuerpo para dos especies de peces Curimata mivartii y Pimelodus grosskopfii en hábitats lóticos y lénticos de la cuenca Colombiana del Magdalena-Cauca. Resultados: Los individuos de C. mivartii de dos hábitats lóticos fueron similares en tamaño y significativamente más grandes que los del hábitat léntico, mientras que los individuos de P. grosskopfii fueron similares en ambos hábitats. Ambas especies mostraron cuerpos más hidrodinámicos en hábitats lóticos y formas corporales más robustas en hábitats lénticos. Conclusión: Ambas especies exhiben cambios fenotípicos en la forma del cuerpo concordantes con las predicciones previas sobre las variaciones morfológicas de los peces. Esta información es relevante para predecir cambios en respuesta a la heterogeneidad ambiental, especialmente inducidos por actividades antropogénicas que cambian las velocidades del agua en el río.


Abstract Introduction: Despite their importance in swimming performance and individual fitness, body shape and size variations in response to water flow velocity remain unknown for most migratory freshwater fishes in the Neotropics. Objective: Test the hypothesis of phenotypic variation in the size and body shape of two species of freshwater fishes that encounter changes in water velocity across their migrations in two rivers that belong to a single Neotropical basin. Methods: Landmark-based geometric morphometrics was used to study variations in body size and shape for the fish species Curimata mivartii and Pimelodus grosskopfii in lotic and lentic habitats of the Colombian Magdalena-Cauca Basin. Results: Individuals of C. mivartii from two lotic habitats were similar in size and both were significantly bigger than those from lentic habitats, whereas individuals of P. grosskopfii were similar in size in both habitats. Both species showed more streamlined bodies in lotic habitats and deeper body shapes in lentic habitats. Conclusions: Both species exhibit phenotypic changes in body shape concordant with previous predictions on morphological variations of fishes. This information is relevant to predict changes in response to environmental heterogeneity, especially those induced by anthropogenic activities that change the water velocity in the river.


Assuntos
Animais , Peixes-Gato/anatomia & histologia , Peixes , Colômbia
8.
Micromachines (Basel) ; 12(3)2021 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-33673667

RESUMO

Despite the very simple geometry and the laminar flow, T-shaped microreactors have been found to be characterized by different and complex steady and unsteady flow regimes, depending on the Reynolds number. In particular, flow unsteadiness modifies strongly the mixing process; however, little is known on how this change may affect the yield of a chemical reaction. In the present work, experiments and 3-dimensional numerical simulations are carried out jointly to analyze mixing and reaction in a T-shaped microreactor with the ultimate goal to investigate how flow unsteadiness affects the reaction yield. The onset of the unsteady asymmetric regime enhances the reaction yield by more than 30%; however, a strong decrease of the yield back to values typical of the vortex regime is observed when the flow undergoes a transition to the unsteady symmetric regime.

9.
Hydrol Process ; 35(2): e14017, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33678948

RESUMO

Beavers can profoundly alter riparian environments, most conspicuously by creating dams and wetlands. Eurasian beaver (Castor fiber) populations are increasing and it has been suggested they could play a role in the provision of multiple ecosystem services, including natural flood management. Research at different scales, in contrasting ecosystems is required to establish to what extent beavers can impact on flood regimes. Therefore, this study determines whether flow regimes and flow responses to storm events were altered following the building of beaver dams and whether a flow attenuation effect could be significantly attributed to beaver activity. Four sites were monitored where beavers have been reintroduced in England. Continuous monitoring of hydrology, before and after beaver impacts, was undertaken on streams where beavers built sequences of dams. Stream orders ranged from 2nd to 4th, in both agricultural and forest-dominated catchments. Analysis of >1000 storm events, across four sites showed an overall trend of reduced total stormflow, increased peak rainfall to peak flow lag times and reduced peak flows, all suggesting flow attenuation, following beaver impacts. Additionally, reduced high flow to low flow ratios indicated that flow regimes were overall becoming less "flashy" following beaver reintroduction. Statistical analysis, showed the effect of beaver to be statistically significant in reducing peak flows with estimated overall reductions in peak flows from -0.359 to -0.065 m3 s-1 across sites. Analysis showed spatial and temporal variability in the hydrological response to beaver between sites, depending on the level of impact and seasonality. Critically, the effect of beavers in reducing peak flows persists for the largest storms monitored, showing that even in wet conditions, beaver dams can attenuate average flood flows by up to ca. 60%. This research indicates that beavers could play a role in delivering natural flood management.

10.
Sci Total Environ ; 654: 948-959, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30841412

RESUMO

Phytoplankton is critical to river ecosystems. These organisms are sensitive to streamflow and water quality changes and, therefore, used to determine stability of river ecosystems, especially in regulated rivers. However, exactly how such disturbances alter spatial distribution of phytoplankton remains unclear, particularly during different seasons. A thorough understanding of these mechanisms is required to better analyze impact of environmental factors on regulated rivers. Given this, phytoplankton communities, streamflow, and water quality factors were assessed in areas sampled four times from 2015 to 2016 in upper and middle Huai River Basin. Biodiversity indices, as well as cluster and rank analyses, were used to (1) determine phytoplankton composition and distribution and (2) clarify impacts of key streamflow and water quality factors on such communities. It was found phytoplankton composition deteriorated over time, with phyla number decreasing from six to three. Moreover, proportion of Bacillariophyta increased from 51.83% to 68.13%. Phytoplankton in three regions, upstream region (Shannon-Wiener index 1.39-2.95), midstream region (0.70-4.55), and downstream region (0.22 to 2.97), were spatially clustered. The most impact factors impacting variation in composition and distribution were water quality factors and then hydrological factors. Of these, the most important factors in wet seasons were total nitrogen and maximum runoff, while ammonia nitrogen and low flow discharge were the most important factors during dry seasons. Streamflow and water quality contributed the most in midstream region, which was significantly affected by numbers of high and low flow. Contributions of these factors to downstream region were the strongest during dry seasons, which were significantly affected by numbers of low flow. Collectively, these results reveal significant impact of streamflow and water quality factors on phytoplankton deterioration in upper and middle Huai River Basin. Critically, this study provides scientific and technological support for increased biomonitoring and ecohydrological studies in regulated river basins.


Assuntos
Biodiversidade , Monitoramento Ambiental , Fitoplâncton/crescimento & desenvolvimento , China , Rios/química , Poluição da Água/análise
11.
Sci Total Environ ; 651(Pt 1): 357-366, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30240919

RESUMO

Changes in land use, climate and flow diversion are key drivers of river flow regime change that may eventually affect freshwater biodiversity and ecosystem functions. However, our knowledge is limited on how the functional features of stream organisms vary along the gradient of hydrological disturbance (i.e. flow regime changes) and how flow regimes mediate the functional features in lowland streams. We analyzed the functional traits of benthic diatoms (unicellular siliceous algae) that are most sensitive and tolerant to flow regime changes along a nationwide scale of 246 sites in Denmark. We combined RLQ and fourth-corner analyses to explore the co-variation between hydrological variables (R table) and species traits (Q table), constrained by the relative abundance of each species (L table) as observed in each of the sampling sites. Further, we examine the relationships between functional features (i.e., functional redundancy and diversity) and hydrological variables by multivariate statistical analyses. Results show that species turnover with displacement of sensitive species by tolerant species was the dominating process in benthic diatom communities during high flow disturbances. Functional features, as indicated by functional diversity and redundancy indices, were mediated mainly by high and low flow magnitude. Median daily flow magnitude shows a consistent positive relationship with functional redundancy and richness indices indicating that larger streams are more resilient to flow perturbations. In addition flow regime changes are less important than median daily flow magnitude and show inconsistent correlation to functional features likely due to the interaction of multiple environmental stressors. Our study highlights the robustness of trait-based approaches for identifying flow regime changes in streams, and strongly suggests that biodiversity conservation and water resource management should focus on protecting natural base flow in headwater streams and generally reduce flow regulation for sustaining stream ecosystems under future global changes.


Assuntos
Biodiversidade , Diatomáceas/fisiologia , Rios , Movimentos da Água , Dinamarca
12.
Bioprocess Biosyst Eng ; 41(6): 757-770, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29428998

RESUMO

Biofilms are found at the inner surfaces of drinking water pipes and, therefore, it is essential to understand biofilm processes to control their formation. Hydrodynamics play a crucial role in shaping biofilms. Thus, knowing how biofilms form, develop and disperse under different flow conditions is critical in the successful management of these systems. Here, the development of biofilms after 4 weeks, the initial formation of biofilms within 10 h and finally, the response of already established biofilms within 24-h intervals in which the flow regime was changed, were studied using a rotating annular reactor under three different flow regimes: turbulent, transition and laminar. Using fluorescence microscopy, information about the number of microcolonies on the reactor slides, the surface area of biofilms and of extracellular polymeric substances and the biofilm structures was acquired. Gravimetric measurements were conducted to characterise the thickness and density of biofilms, and spatial statistics were used to characterise the heterogeneity and spatial correlation of biofilm structures. Contrary to the prevailing view, it was shown that turbulent flow did not correlate with a reduction in biofilms; turbulence was found to enhance both the initial formation and the development of biofilms on the accessible surfaces. Additionally, after 24-h changes of the flow regime it was indicated that biofilms responded to the quick changes of the flow regime. Overall, this work suggests that different flow conditions can cause substantial changes in biofilm morphology and growth and specifically that turbulent flow can accelerate biofilm growth in drinking water.


Assuntos
Biofilmes/crescimento & desenvolvimento , Água Potável/microbiologia , Modelos Biológicos , Microbiologia da Água
13.
J Exp Biol ; 220(Pt 12): 2265-2276, 2017 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-28396354

RESUMO

Fishes use their mechanoreceptive lateral line system to sense nearby objects by detecting slight fluctuations in hydrodynamic motion within their immediate environment. Species of fish from different habitats often display specialisations of the lateral line system, in particular the distribution and abundance of neuromasts, but the lateral line can also exhibit considerable diversity within a species. Here, we provide the first investigation of the lateral line system of the Australian western rainbowfish (Melanotaenia australis), a species that occupies a diversity of freshwater habitats across semi-arid northwest Australia. We collected 155 individuals from eight populations and surveyed each habitat for environmental factors that may contribute to lateral line specialisation, including water flow, predation risk, habitat structure and prey availability. Scanning electron microscopy and fluorescent dye labelling were used to describe the lateral line system in M. australis, and to examine whether the abundance and arrangement of superficial neuromasts (SNs) varied within and among populations. We found that the SNs of M. australis were present in distinct body regions rather than lines. The abundance of SNs within each body region was highly variable, and also differed among populations and individuals. Variation in SN abundance among populations was best explained by habitat structure and the availability of invertebrate prey. Our finding that specific environmental factors explain among-population variation in a key sensory system suggests that the ability to acquire sensory information is specialised for the particular behavioural needs of the animal.


Assuntos
Peixes/anatomia & histologia , Sistema da Linha Lateral/anatomia & histologia , Animais , Austrália , Feminino , Sistema da Linha Lateral/ultraestrutura , Masculino , Microscopia Acústica , Microscopia de Fluorescência , Movimentos da Água , Austrália Ocidental
14.
Water Resour Res ; 53(12): 10274-10292, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30333671

RESUMO

We imaged the steady state flow of brine and decane in Bentheimer sandstone. We devised an experimental method based on differential imaging to examine how flow rate impacts impact the pore-scale distribution of fluids during coinjection. This allows us to elucidate flow regimes (connected, or breakup of the nonwetting phase pathways) for a range of fractional flows at two capillary numbers, Ca, namely 3.0 × 10-7 and 7.5 × 10-6. At the lower Ca, for a fixed fractional flow, the two phases appear to flow in connected unchanging subnetworks of the pore space, consistent with conventional theory. At the higher Ca, we observed that a significant fraction of the pore space contained sometimes oil and sometimes brine during the 1 h scan: this intermittent occupancy, which was interpreted as regions of the pore space that contained both fluid phases for some time, is necessary to explain the flow and dynamic connectivity of the oil phase; pathways of always oil-filled portions of the void space did not span the core. This phase was segmented from the differential image between the 30 wt % KI brine image and the scans taken at each fractional flow. Using the grey scale histogram distribution of the raw images, the oil proportion in the intermittent phase was calculated. The pressure drops at each fractional flow at low and high flow rates were measured by high-precision differential pressure sensors. The relative permeabilities and fractional flow obtained by our experiment at the mm-scale compare well with data from the literature on cm-scale samples.

15.
Environ Res Lett ; 12(5)2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-30377438

RESUMO

We performed a twofold intercomparison of river discharge regulated by dams under multiple meteorological forcings among multiple global hydrological models for a historical period by simulation. Paper II provides an intercomparison of river discharge simulated by five hydrological models under four meteorological forcings. This is the first global multimodel intercomparison study on dam-regulated river flow. Although the simulations were conducted globally, the Missouri-Mississippi and Green- Colorado Rivers were chosen as case-study sites in this study. The hydrological models incorporate generic schemes of dam operation, not specific to a certain dam. We examined river discharge on a longitudinal section of river channels to investigate the effects of dams on simulated discharge, especially at the seasonal time scale. We found that the magnitude of dam regulation differed considerably among the hydrological models. The difference was attributable not only to dam operation schemes but also to the magnitude of simulated river discharge flowing into dams. That is, although a similar algorithm of dam operation schemes was incorporated in different hydrological models, the magnitude of dam regulation substantially differed among the models. Intermodel discrepancies tended to decrease toward the lower reaches of these river basins, which means model dependence is less significant toward lower reaches. These case-study results imply that, intermodel comparisons of river discharge should be made at different locations along the river's course to critically examine the performance of hydrological models because the performance can vary with the locations.

16.
Cell Biochem Biophys ; 74(2): 241-52, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26983824

RESUMO

Paramecium or other ciliates have the potential to be utilized for minimally invasive surgery systems, making internal body organs accessible. Paramecium shows interesting responses to changes in the concentration of specific ions such as K(+), Mg(2+), and Ca(2+) in the ambient fluid. Some specific responses are observed as, changes in beat pattern of cilia and swimming toward or apart from the ion source. Therefore developing a model for chemotactic motility of small organisms is necessary in order to control the directional movements of these microorganisms before testing them. In this article, we have developed a numerical model, investigating the effects of Ca(2+) on swimming trajectory of Paramecium. Results for Ca(2+)-dependent chemotactic motility show that calcium gradients are efficient actuators for controlling the Paramecium swimming trajectory. After applying a very low Ca(2+) gradient, a directional chemotaxis of swimming Paramecium is observable in this model. As a result, chemotaxis is shown to be an efficient method for controlling the propulsion of these small organisms.


Assuntos
Cálcio/farmacologia , Quimiotaxia/efeitos dos fármacos , Modelos Biológicos , Paramecium/citologia , Paramecium/efeitos dos fármacos , Relação Dose-Resposta a Droga , Movimento/efeitos dos fármacos , Paramecium/fisiologia
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