[Improvement of the pre-examination phase of medical biology exams at the Henri Mondor University Hospitals: a pilot study]. / Une étude pilote pour l'amélioration de l'étape pré-analytique des examens de biologie médicale pris en charge au sein des hôpitaux universitaires Henri Mondor.

The medical and university department of biology pathology at Henri Mondor hospital in Créteil has been engaged in an NF EN ISO 15189 accreditation process since 2014. One of the elements of this process concerns the quality of handling of samples and their transportation to laboratories, including the implementation place requires fighting against pre-examination non-conformities, which are the source of many dysfunctions. The pre-examination group has implemented several actions in a targeted care service. Thanks to these, the rate of non-conformities has halved in 18 months. In parallel, a work project targeting student nurses on internship was born to follow up on the results of a statistical study carried out by the pre-examination group on non-conformities. The objective of the project was to include nursing students on internship in a full support course on good sampling practices and pre-analytical non-conformities. This was based on the realization of two knowledge quizzes (before and after training), theoretical training, and visits to several laboratories. This study lasted 10 months with the participation of 37 students. The results showed a marked improvement in knowledge of pre-analytics as well as total satisfaction of all students. Our approach has helped to better understand the needs of laboratories and demonstrates the usefulness of training students in good sampling practices in order to ensure better patient care as well as an improvement in their comfort and well-being.

The Effect of Barcode Technology Use on Pathology Specimen Labeling Errors.

Pathology specimen labeling errors occur for a variety of reasons. We investigated the use of barcode technology as a method to improve the accuracy of pathology specimen labeling and patient safety. We also assessed nurses' perceptions of system quality, information quality, service quality, user satisfaction, and net benefits. Sixty-eight perioperative nurses who work in a teaching hospital in Taiwan completed the survey. Nurses scored net benefits as highly contributing to their satisfaction, whereas system quality contributed most to dissatisfaction. Further, we analyzed pathology specimen records before and after implementing the barcode system and found that specimen management errors significantly decreased. The use of a reliable barcode system could improve specimen labeling accuracy and enhance nurses' satisfaction with this technology.

Urinary Sample Collection Methods in Ileal Conduit Urinary Diversion Patients: A Randomized Control Trial.

PURPOSE: The purpose of this study was to compare bacteriological urinalysis findings using 3 urinary sample collection methods (clean stoma catheterization, urine dripping from the stoma, urine collected from the clean urostomy pouch) in ileal conduit urinary diversion patients. DESIGN: Randomized controlled trial. SAMPLE AND SETTING: Twenty-seven patients with ileal conduit urinary diversion from an outpatient urology clinic were enrolled; 9 patients were seen twice, for a total of 36 subjects and comparisons. METHODS: Data were collected during a clinic visit by a trained research nurse. Patients were randomized into 2 groups: group A had the first urine sample collected by clean stoma catheterization, followed by sample collection by urine dripping from the stoma; group B had the first urine sample collection by urine dripping from the stoma, followed by sample collected by clean stoma catheterization. All patients had a third urine sample collected from a factory-clean urostomy pouch. Bacteriological urinalysis findings were compared among methods. Descriptive analyses were summarized using mean, percentage, and frequency. The mean ages of the patients between the groups were compared with the t test. Other between-group comparisons were performed using the Fisher exact test. Urinary culture finding differences among the same patients were evaluated using the McNemar test. Sensitivity and specificity of the different urine sample collection methods were calculated assuming urine sample collection by catheterization as a reference method. RESULTS: Uropathogen bacteria were detected in urinary culture in 16 of 36 samples (44%) collected by clean stoma catheterization, 15 of 36 samples (42%) collected by urine dripping directly from the stoma, and 13 of 35 samples (37%) collected from the clean urostomy pouch. Significant differences among the urine collection methods were not detected. Assuming catheterization as the most reliable method of sample collection, the sensitivity and specificity of the urine dripping from stoma collection method were 81.3% and 90.0%, respectively. The sensitivity and specificity of the urostomy pouch collection method were 73.3% and 90.0%, respectively. Among the same patients, there were no significant differences in the incidence of uropathogen bacteria when clean stoma catheterization was compared with urine dripping from the stoma and urostomy pouch methods. CONCLUSION: This study provides clinically relevant information regarding urine collection methods in ileal conduit patients. Urinary sample collection by urine dripping directly from the stoma or collected from a clean urostomy pouch provided similar uropathogen bacteria findings compared with sample collection by clean stoma catheterization.

Maternal-Child Microbiome: Specimen Collection, Storage, and Implications for Research and Practice.

BACKGROUND: The maternal microbiome is a key contributor to the development and outcomes of pregnancy and the health status of both mother and infant. Significant advances are occurring in the science of the maternal and child microbiome and hold promise in improving outcomes related to pregnancy complications, child development, and chronic health conditions of mother and child. OBJECTIVES: The purpose of this study was to review site-specific considerations in the collection and storage of maternal and child microbiome samples and its implications for nursing research and practice. APPROACH: Microbiome sampling protocols were reviewed and synthesized. Precautions across sampling protocols were also noted. RESULTS: Oral, vaginal, gut, placental, and breast milk are viable sources for sampling the maternal and/or child microbiome. Prior to sampling, special considerations need to be addressed related to various factors including current medications, health status, and hygiene practices. Proper storage of samples will avoid degradation of cellular and DNA structures vital for analysis. DISCUSSION: Changes in the microbiome throughout the perinatal, postpartum, and childhood periods are dramatic and significant to outcomes of the pregnancy and the long-term health of mother and child. Proper sampling techniques are required to produce reliable results from which evidence-based practice recommendations will be built. Ethical and practical issues surrounding study design and protocol development must also be considered when researching vulnerable groups such as pregnant women and infants. Nurses hold the responsibility to both perform the research and to translate findings from microbiome investigations for clinical use.

[Pre-analytical errors in blood sampling: an observational study on specimen collection technique and laboratory results]. / Gli errori preanalitici nel prelievo ematico: uno studio osservazionale sulle modalità di raccolta dei campioni e sugli esiti di laboratorio.

INTRODUCTION: Around 60-70% of diagnostic and therapeutic decisions are based on blood exams. Several errors occur during the pre-analytic phase. AIMS: of this study were: to describe nurses' behaviours in blood specimen collection; to describe prevalence and type of pre-analytical errors; to assess the association between pre-analytical errors and occurrence of unsuitable specimens. METHODS: An observational cross-sectional study was conducted by means of a structured form based on up-to-date clinical recommendations. A researcher observed nurses' behaviors during 172 blood sampling procedures in medical, surgical and emergency care settings. Unsuitable procedures were registered. RESULTS: Most behaviours were correct, however some significantly diverged from recommended practices: active and passive patient identification; respect of antiseptic solution's drying time; rapid removal of tourniquet when blood started flowing. The prevalence of unsuitable specimen reports was significantly higher when the procedure involved a small calibre vein (RP: 0.19; IC95% 0.04 - 0.98; p = 0.03) and when blood drawing was difficult (RP; 3.83; IC95% 1.63 - 9.01; p <0.001). CONCLUSIONS: The pre-analytical phase is important for the diagnostic process and safety of patients. Although some factors ­ as patients' characteristics ­ are non-modifiable, some nurses' behaviours could be improved to reduce risk of pre-analytical errors. Further studies are needed to clarify the associations between pre-analytical errors and laboratory outcomes.

[Can a blood sample for diagnostic exams be drawn from a peripheral venous catheter?]. / Si può eseguire un prelievo a scopo diagnostico da un catetere venoso periferico?

INTRODUCTION: Often the blood sample in patients exposed to serial blood exams is taken from the venous cannula (PVC) to avoid a second venipuncture. AIM: To assess whether blood draws from PVCs are more haemolized than those drawn from phlebotomy, and if the blood drawn from a cannula interferes with the test results. METHODS: A bibliographic search was conducted in PubMed, Cinhal and Ovid, with the following key words: Peripheral Catheter, Blood Specimen Collection, Blood Sample, Haemolysis, 19 articles were identified (including 2 meta analyses), selecting the articles involving emergency departments. RESULTS: The meta-analyses agree on the lower risk for haemolysis in blood drawn from a needle: risk reduction of 84% (RR=0.16, IC 95% 0.11-0.24) (Heyer et al); the blood draw from PVC increased the risk for hemolysis of 7% (RR 1.07 IC 95% 1.06-1.08, p<0.001) (Lippi et al). Manual aspiration reduces the risk for hemolysis compared to the vacutainer equipment. However, the results of tests drawn from PVC are not significantly different compared to the classical phlebotomy. CONCLUSIONS: Blood draws from PVC bear an higher risk for heamolysis but can be safely used for most diagnostic exams, with the exception of blood gase.

Biospecimen collection, processing, and analysis: new challenges for oncology nurses.

OBJECTIVES: To provide an overview of emerging applications for and challenges associated with biospecimen collection for evaluating personalized disease risk and/or treatment response in cancer clinical trials. DATA SOURCES: Published nursing and medical literature. CONCLUSION: Blood- and tissue-based biomarkers are increasingly utilized to identify the molecular signatures of disease that can inform the determination of a course of treatment in a very precise manner. There are challenges for the oncology nurse related to specimen collection, processing, analysis, and translation to precision treatment. IMPLICATIONS FOR NURSING PRACTICE: It is important for nurses to have appropriate training and a working understanding of the procedures for biospecimen collection and how biospecimen analyses can inform precision assessment of risk and prognosis.

Fiabilidad de los resultados analíticos de muestra sanguínea desechando el doble del espacio muerto del catéter / Reliability of laboratory findings from blood samples when only the double dead space in catheter is discarded

OOBJETIVO: determinar la fiabilidad de dos métodos de extracción desangre a través de catéter arterial en los pacientes ingresados en una unidad de reanimación postquirúrgica. MATERIAL Y MÉTODO: estudio cuasi experimental realizado en el Hospital de la Princesa de Madrid, de febrero a mayo de 2010, en el que se compararon dos métodos de extracción de muestra de sangre para análisis a través de catéter arterial periférico, realizados secuencialmente: primero la intervención= 2 cc sangre desechada + 13,5 muestra a analizar, y segundo el control= inmediatamente después de la extracción previa= 15,5 cc + 13,5 muestra a analizar, en relación a los resultados de parámetros analíticos bioquímicos, hematológicos y de coagulación. RESULTADOS: la diferencia de las medias es muy pequeña en todos los parámetros estudiados; en todos los casos el "0" se encuentra incluido en los respectivos intervalos de confianza, no siendo estadísticamente significativos (p> 0,05). Las correlaciones son superiores a 0,93 en todos los parámetros estudiados en el caso de la bioquímica, superiores a0,84 en el caso de la hematimetría y superiores a 0,90 en el caso de la coagulación, obteniendo en todos los casos unos valores de p< de 0,05.CONCLUSIONES: para garantizar la calidad de los resultados de laboratorio es suficiente desechar el volumen correspondiente al doble del espacio muerto del sistema (en el presente estudio 2 cc)

PURPOSE: to assess reliability of two blood sample drawing methods though an arterial catheter in patients admitted to a postoperative resuscitation unit. MATERIAL AND METHODS: a quasi-experimental study was carried out at Hospital de la Princesa, Madrid, Spain, from February to May 2010.Two sequentially used methods for drawing blood samples for laboratory tests through a peripheral arterial catheter were compared: firstly the intervention procedure = 2 cc of blood discarded + a 13.5 sample for laboratory testing; and secondly the control procedure = immediately after previous sample drawing = 15.5 cc + a 13.5 sample for laboratory testing. Biochemistry, hematology and coagulation laboratory parameters were compared. RESULTS: differences in mean values were very low for all assessed parameters. Zero value is always included in confidence intervals; therefore, differences were not statistically significant (p > 0.05). Correlation is higher than 0.93 for all assessed biochemistry parameters, higher than 0.84 for hematology parameters, and higher than 0.90 for coagulation parameters (p < 0.05 for all comparisons).CONCLUSIONS: discarding the volume corresponding to the doubledead space in the system (2 cc in our study) is adequate to ensure quality in laboratory testing results

Extracción de muestras sanguíneas en el paciente crítico. Práctica enfermera / Extraction of blood samples in the critical patient

Objetivo. Descripción de la práctica habitual de los profesionales de enfermería en la extracción de muestras sanguíneas en el paciente crítico. Material y métodos. Estudio observacional, descriptivo y transversal realizado durante una semana de noviembre del 2010. Noventa enfermeras de cuidados intensivos participaron en el estudio donde se recogían datos referentes a la práctica habitual de estos profesionales. Resultados. Cuando los pacientes son portadores de un acceso arterial, las enfermeras eligen esta vía para la extracción de muestras sanguíneas. El volumen que se extrae, previa obtención de la muestra, y el volumen de la muestra que se ha de analizar dependen de la unidad de trabajo. Un 94,2 % de las enfermeras considera que se trata de un tema relevante. Conclusiones. El volumen de sangre extraída previa obtención de la muestra de laboratorio y la muestra extraída para analizar es superior al mínimo recomendado. No existiendo protocolos en esta línea de actuación. Un resumen del trabajo fue presentado en formato póster en el 11th Annual NATA Symposium celebrado en Barcelona los días 8 y 9 de abril de 2010: «Evaluation of the competence of nursing professionals in blood extractions in the critical patient» (AU)

Objective: To evaluate the competence of nursing in blood extractions in the Intensive Care. Material and Methods: Transversal descriptive study was done during one week of November 2010. 90 nurses working in intensive care units participate in the study. We collected information related to the normal practice. Results: When the patient has an arterial catheter, the nurses choose this way to extract the blood samples. The volume of blood extracted through the arterial catheter before taking the sample, through a venous catheter and the volume of blood that is taken for an arterial blood gas analysis depends of the nurse unit. A 94,2 % of the nurses think that it is a relevant item but there were no protocols. Conclusion: The volume of blood extracted previous obtaining the sample, and the blood taking for analysis is higher than the minimum recommended. There are no protocols in this line (AU)

Procedure for obtaining a urine sample from a urostomy, ileal conduit, and colon conduit: a best practice guideline for clinicians.

The purpose of this document was to define the correct technique for obtaining a urine sample from a urostomy, ileal, or colon conduit. While healthcare providers do not commonly encounter patients with a urostomy, knowledge of the correct procedure to obtain a urine specimen is essential. Urine samples obtained incorrectly from a urostomy can lead to inaccurate cultures, resulting in an improper diagnosis and treatment, which can endanger the life of a patient. This column presents patient preparation, the procedure to obtain a specimen with and without a catheter, and aftercare of the patient and specimen. This best practice guideline has been developed by a panel of certified ostomy nurses serving on the Wound, Ostomy and Continence Nurses (WOCN) Society's Clinical Practice Ostomy Committee. The guideline has undergone content validation through a consensus-building process by the WOCN Society, which was managed by the Center for Clinical Investigation.

Comparison of two methods for acquisition of sputum samples for diagnosis of suspected tuberculosis in smear-negative or sputum-scarce people: a randomised controlled trial.

BACKGROUND: Sputum obtained either under instruction from a health-care worker or through induction can improve case detection of active tuberculosis. However, the best initial sputum sampling strategy for adults with suspected smear-negative or sputum-scarce tuberculosis in primary care is unclear. We compared these two methods of sample acquisition in such patients. METHODS: In this randomised controlled trial, we enrolled adults (age ≥18 years) with sputum-scarce or smear-negative suspected tuberculosis from three primary care clinics in Cape Town, South Africa. Patients were randomly assigned (1:1) to receive either health-care worker instruction or induction to obtain sputum samples. Neither patients nor investigators were masked to allocation. The primary outcome was the proportion of patients who had started treatment after 8 weeks in a modified intention-to-treat population. Secondary outcomes were proportions starting treatment within different time periods, proportion of patients producing sputum for diagnosis, adverse effects, sputum samples' quality, and case detection by diagnostic method. This study is registered with ClinicalTrials.gov, number NCT01545661. FINDINGS: We enrolled 481 patients, of whom 213 were assigned to health-care worker instruction versus 268 assigned to induction. The proportion of patients who started treatment in the 8 weeks after enrolment did not differ significantly between groups (53/213 [25%] vs 73/268 [27%]; OR 0·88, 95% CI 0·57-1·36; p=0·56). A higher proportion of instructed versus induced patients initiated empiric treatment based on clinical and radiography findings (32/53 [60%] vs 28/73 [38%]; p=0·015). An adequate sputum sample ≥1 mL was acquired in a lower proportion of instructed versus induced patients (164/213 [77%] vs 238/268 [89%]; p<0·0001), and culture-based diagnostic yield was lower in instructed versus induced patients (24/213 [11%] vs 51/268 [19%]; p=0·020). However, same-day tuberculosis case detection was similar in both groups using either smear microscopy (13/213 [6%] vs 22/268 [8%]; p=0·38) or Xpert-MTB/RIF assay (13/89 [15%] vs 20/138 [14%]; p=0·98). No serious adverse events occurred in either group; side-effects related to sample acquisition were reported in 32 of 268 (12%) patients who had sputum induction and none who had instruction. Cost per procedure was lower for instructed than for induced patients (US$2·14 vs US$7·88). INTERPRETATION: Although induction provides an adequate sample and a bacteriological diagnosis more frequently than instruction by a health-care worker, it is more costly, does not result in a higher proportion of same-day diagnoses, and-because of widespread empiric treatment-may not result in more patients starting treatment. Thus, health-care worker instruction might be the preferred strategy for initial collection of sputum samples in adults with suspected sputum-scarce or smear-negative tuberculosis in a high burden primary care setting. FUNDING: South African National Research Foundation, European Commission, National Institutes of Health, European and Developing Countries Clinical Trials Partnership, Discovery Foundation.

Cerebrospinal fluid collection: a comparison of different collection sites on the external ventricular drain.

BACKGROUND: Intracranial pressure monitoring using an external ventricular drainage (EVD) system is the most commonly used technology to monitor intracranial pressure or drain cerebrospinal fluid (CSF) in neurological and neurosurgical patients. CSF samples are collected routinely from the EVD system for laboratory tests. No study has been conducted to identify where the most appropriate site for CSF collection is in order to reduce the disruption of the closed EVD system and reduce the risk of infection. PURPOSE: The purpose of this study was to identify a CSF sampling port in the EVD system that is easily accessible, provides accurate results, and minimizes disruption to the closed EVD system. SAMPLE: Fifty patients admitted to the neurological and neurosurgical intensive care step-down unit with the EVD system between July 2007 and September 2009 agreed to participate in the study. There were 21 women and 29 men. Forty-seven patients' data were analyzed. METHOD: The design was quasi-experimental using a convenience sample. Two samples of CSF were collected daily. One sample was collected from the proximal port and another sample was collected from the distal port. The second sample was collected immediately after the first. Each set of samples (proximal and distal) was tested and compared for any differences in appearance, culture results, and concentrations of protein, glucose, and white cell count. RESULTS: Using a two-tailed paired t test with 95% confidence interval, there was no statistically significant difference between the samples obtained from the two collection sites for protein, glucose, white cell count, appearance, and culture. Pearson's correlation coefficient was also used to analyze the correlation for the continuous measures. Both protein and glucose had very high correlations. However, the white cell count, and white cell counts and culture had very low correlations. CONCLUSION: The distal port of the EVD system is safe and easy for CSF collection. It also provides accurate results for CSF samples. When the CSF sample is collected from the distal port, the entire volume of CSF in the drip chamber should be collected and tested to obtain an accurate WBC count per unit of volume.

[The taking and transport of biological samples]. / Krankenhaushygiene - Abnahme und Transport von mikrobiologischen Proben.

The results of microbiological tests are the foundation for a targetted therapy and the basis for monitoring infections. The quality of each and every laboratory finding depends not only on an error-free analytical process. The pre-analysis handling procedures are of particular importance. They encompass all factors and influences prior to the actual analysis. These include the correct timepoint for sample taking, the packaging and the rapid transport of the material to be investigated. Errors in the pre-analytical processing are the most frequent reasons for inappropriate findings.

Estimating intra- and inter-assay variability in salivary cortisol.

Investigators commonly assess intra- and inter-assay coefficients of variation (CVs) to estimate the precision of salivary cortisol enzyme immunoassay (EIA). However, little guidance is available as to which samples to use for CV assessment. The purposes of this methodological study were to compare differences in intra- and inter-assay CVs (a) among controls, standards, and/or unknown samples; and (b) between fresh and previously frozen saliva. A total of 174 duplicates (controls = 58, standards = 48, and unknowns = 68) were tested. The unknowns were from 34 students; all student saliva was assayed as both fresh and frozen samples. All samples were assayed in duplicate, using a commercial salivary cortisol EIA kit, by the same technician with the same equipment. A priori criteria for intra- and inter-assay CV, respectively, were ≤ 4% and ≤ 7%, and a was .05 for CV differences. Mean intra-assay CVs for controls, standards, unknowns, and combined samples were ≤ 2.5%, and mean inter-assay CVs were ≤ 2.8%. Mean intra-assay CVs were 2.2% for fresh saliva and 1.5% for frozen samples. Comparisons showed no significant differences in intra- or inter-assay CV among controls, standards, and/or unknown samples. Inter-assay CV was significantly different between fresh and previously frozen saliva (p = .043), with fresh saliva CV higher than frozen; the difference was not meaningful because all evaluations showed minimal measurement error. In conclusion, results indicate that estimation of precision can be achieved by testing of controls, standards, or unknowns and with either fresh or frozen saliva in this population.

[A medical test laboratory specialised in pediatrics]. / Un laboratoire d'analyses médicales spécialisé en pédiatrie.

A medical test laboratory in Paris specialises in children's biological samples. Renowned for its practice of collecting samples from children without difficulty and for its medicalised and paediatric interpretation, the laboratory's main objective is to ensure the quality and comfort of the healthcare it provides. A visit alongside the laboratory's founder, a paediatrician-biologist, and a nurse who has chosen to work there.