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1.
Carbohydr Polym ; 300: 120232, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36372472

RESUMO

Herein, a facile method to fabricate hierarchical super-elastic (SE) sponge using a water-soluble cellulose derivative, carboxymethyl cellulose (CMC), is reported. The method includes ice templating and porogen leaching steps which facilitate to generate macro-sized pores as well as pore wall structures that can dissipate stress effectively. By controlling the porogen content, the specific surface area and the morphology of the sponges can be tuned. Furthermore, a plasticizing method was used before vacuum drying to reduce the deformation of the inner structure. The derived hierarchical SE CMC sponges exhibit excellent fatigue resistance, fast shape recovery, high-water absorption, biosafeness, and fast degradation. Thus, our strategy provides a novel method for the construction of SE sponges which show great potential in green elastic wound dressing, tissue engineering, and absorbent materials.


Assuntos
Bandagens , Carboximetilcelulose Sódica , Celulose/química , Engenharia Tecidual , Água/química
2.
J Biomed Mater Res B Appl Biomater ; 111(1): 220-233, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35959858

RESUMO

The skin is the largest organ in the human body and its physical integrity must be maintained for body homeostasis and to prevent the entry of pathogenic microorganisms. Sodium alginate (SA) and polyvinyl alcohol (PVA) are two polymers widely used in films for wound dressing applications. Furthermore, blends between SA and PVA improve physical, mechanical and biological properties of the final wound healing material when compared to the individual polymers. Different drugs have been incorporated into SA/PVA-based films to improve wound healing activity. It is noteworthy that SA/PVA films can be crosslinked with Ca2+ or other agents, which improves physicochemical and biological properties. Thus, SA/PVA associations are promising for the biomedical field, as a potential alternative for wound treatment. This review focuses on the main techniques for obtaining SA/PVA films, their physical-chemical characterization, drug incorporation, and the advantages and challenges of these films for wound healing.


Assuntos
Alginatos , Álcool de Polivinil , Humanos , Álcool de Polivinil/química , Alginatos/química , Cicatrização , Bandagens , Polímeros
3.
Sci Rep ; 12(1): 18541, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329229

RESUMO

Cell sheet engineering, a scaffold-free approach to fabricate functional tissue constructs from several cell monolayers, has shown promise in tissue regeneration and wound healing. Unfortunately, these cell sheets are often too small to provide sufficient wound area coverage. In this study, we describe a process to enlarge cell sheets using MEEK micrografting, a technique extensively used to expand skin autografts for large burn treatments. Human dermal fibroblast cell sheets were placed on MEEK's prefolded gauze without any use of adhesive, cut along the premarked lines and stretched out at various expansion ratios (1:3, 1:6 and 1:9), resulting in regular distribution of many square islands of fibroblasts at a much larger surface area. The cellular processes essential for wound healing, including reattachment, proliferation, and migration, of the fibroblasts on expanded MEEK gauze were superior to those on nylon dressing which served as a control. The optimal expansion ratio with the highest migration rate was 1:6, possibly due to the activation of chemical signals caused by mechanical stretching and an effective intercellular communication distance. Therefore, the combination of cell sheet engineering with the MEEK micrografting technique could provide high quality cells with a large coverage area, which would be particularly beneficial in wound care applications.


Assuntos
Queimaduras , Transplante de Pele , Humanos , Transplante de Pele/métodos , Queimaduras/cirurgia , Cicatrização , Bandagens , Fibroblastos
4.
Sci Rep ; 12(1): 18658, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36333591

RESUMO

This study focuses on obtaining and characterizing novel chitosan-based biomaterials containing cannabis oil to potentially promote wound healing. The primary active substance in cannabis oil is the non-psychoactive cannabidiol, which has many beneficial properties. In this study, three chitosan-based films containing different concentrations of cannabis oil were prepared. As the amount of oil increased, the obtained biomaterials became rougher as tested by atomic force microscopy. Such rough surfaces promote protein adsorption, confirmed by experiments assessing the interaction between human albumin with the obtained materials. Increased oil concentration also improved the films' mechanical parameters, swelling capacity, and hydrophilic properties, which were checked by the wetting angle measurement. On the other hand, higher oil content resulted in decreased water vapour permeability, which is essential in wound dressing. Furthermore, the prepared films were subjected to an acute toxicity test using a Microtox. Significantly, the film's increased cannabis oil content enhanced the antimicrobial effect against A. fischeri for films in direct contact with bacteria. More importantly, cell culture studies revealed that the obtained materials are biocompatible and, therefore, they might be potential candidates for application in wound dressing materials.


Assuntos
Cannabis , Quitosana , Humanos , Bandagens/microbiologia , Cicatrização , Materiais Biocompatíveis/farmacologia
5.
Zhonghua Shao Shang Za Zhi ; 38(11): 1079-1084, 2022 Nov 20.
Artigo em Chinês | MEDLINE | ID: mdl-36418266

RESUMO

Objective: To evaluate the interventional effects of music therapy on pain and anxiety of burn patients in wound dressing change. Methods: The meta-analysis method was adopted. Databases including China National Knowledge Internet, Wanfang Database, and VIP database were retrieved with the search terms in Chinese version of ", , /, /", and PubMed, Embase, and Cochrane Library were retrieved with the search terms in English version of "music, burn, dressing change/wound dressing, pain/ache/sore" to obtain the publicly published randomized controlled trials on the application of music therapy for wound dressing change in burn patients from the establishment of each database to May 2021. The outcome indexes included pain score/percentage and anxiety score after dressing change. Rev Man 5.4 and Stata 14.0 statistical software were used to conduct a meta-analysis of eligible studies. Results: A total of 520 burn patients from 7 studies were included, including 260 patients in music therapy group who received music therapy and 260 patients in routine dressing change group who received routine dressing change. The bias risk of all the 7 included studies was uncertain. Compared with those in routine dressing change group, the pain percentages (relative risk=0.06, 95% confidence interval=0.01-0.41, P<0.01) and pain scores after dressing change (standardized mean difference (SMD)=-0.91, 95% confidence interval=-1.61--0.22, P<0.05) of patients in music therapy group were significantly lower. Subgroup analysis showed that music type and timing of intervention might be the source of heterogeneity in pain scores after dressing change. The anxiety scores of patients in music therapy group were significantly lower than those in routine dressing change group (SMD=-0.64, 95% confidence interval=-1.09--0.19, P<0.01). There was no publication bias in pain or anxiety scores after dressing change. Conclusions: Music therapy can relieve the pain and anxiety of burn patients during dressing change.


Assuntos
Queimaduras , Musicoterapia , Humanos , Musicoterapia/métodos , Dor/etiologia , Ansiedade/terapia , Queimaduras/complicações , Queimaduras/terapia , Bandagens
6.
ACS Appl Mater Interfaces ; 14(46): 51630-51644, 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36375077

RESUMO

Postoperative infected wound complications caused by residual tumor cells, bacterial biofilms, and drug-resistant bacteria have become the main challenge in postsurgical skin regeneration. Herein, a bionic cellulose nanocrystal (CNC)-based in situ intelligent wound dressing with near-infrared (NIR)-, temperature-, and pH-responsive functions was designed by using NIR-responsive CNC as the network skeleton, dynamic imine bonds between dialdehyde cellulose nanocrystals and doxorubicin, chitosan oligosaccharide as the pH-responsive switch, and temperature-sensitive poly(N-isopropyl acrylamide) as the temperature-responsive in situ formation switch. The as-prepared wound dressing with the intertwining three-dimensional (3D) network structure possessed high drug loadability of indocyanine green (30 mg/g) and doxorubicin (420 mg/g) simultaneously. The temperature-, NIR-, and pH-responsive switches endowed the wound dressing with controllable on-demand drug release behavior. In particular, the temperature switch endowed the dressing with a shape-adaptable ability on irregularly infected wounds. Interestingly, the wound dressing showed excellent antitumor activity for A375 tumor cells, antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and bacterial biofilm removal ability. Therefore, the developed wound dressing can provide an ideal synergistic treatment strategy combined with chemotherapy and photodynamic and photothermal therapy for postoperative drug-resistant bacteria-infected wound healing.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Nanopartículas , Infecção dos Ferimentos , Humanos , Bandagens , Infecção dos Ferimentos/tratamento farmacológico , Cicatrização , Celulose/farmacologia , Celulose/química , Antibacterianos/química , Bactérias , Doxorrubicina/farmacologia , Hidrogéis/química
7.
J Mater Chem B ; 10(45): 9413-9423, 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36377727

RESUMO

It is of utmost importance that bleeding should be stopped and infection be prevented in people with trauma. In this study, an anisotropic Janus mesoporous silica nanosheet (MSNS) with different functional groups was designed and prepared. In order to endow both sides of the MSNS with independent fast hemostasis and effective antibacterial action, the MSNS was modified with cardanol (CA) and 2,3-epoxypropyltrimethylammonium (GTA). The addition of CA significantly improved the hemostatic property of the MSNS. In a SD rat femoral artery injury model, the hemostatic time of CA-MSNS-GTA was 47% shorter than that of the MSNS, attributed to the sealing of the hydrophobic alkyl side chain and the adhesion of phenolic hydroxyl groups in CA. CA-MSNS-GTA could form a three-dimensional network with fibrin to further accelerate the coagulation process. This Janus material exhibited excellent antibacterial effects (∼90%) against Gram-positive bacteria (S. pneumoniae) and Gram-negative bacteria (E. coli) due to the presence of GTA. The cytotoxicity test showed that CA-MSNS-GTA exhibited biosafety, which provided safety guarantee for clinical applications in the future. This Janus dressing with different functions on two opposite sides provides synergetic multifunctional effects during wound healing.


Assuntos
Hemostáticos , Ratos , Animais , Hemostáticos/farmacologia , Escherichia coli , Ratos Sprague-Dawley , Dióxido de Silício/química , Bandagens , Antibacterianos/farmacologia , Antibacterianos/química , Compostos de Amônio Quaternário/farmacologia , Coagulação Sanguínea
8.
Adv Skin Wound Care ; 35(12): 1-3, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36409191

RESUMO

ABSTRACT: The treatment of chronic wounds remains a serious medical and social problem. There are many innovative therapeutic approaches for the treatment of chronic wounds, one of which is cold plasma therapy. This case report describes an 85-year-old patient who presented with a chronic wound on his lower right leg. Despite repeated surgical wound debridements, his wound did not improve. Therefore, the authors initiated cold plasma therapy for his chronic wound. Through repeated application of cold plasma, the wound was completely healed within 8 weeks. As a noninvasive procedure, the cold plasma treatment was well tolerated and had no adverse effects. This case report provides promising results and could be the basis for further studies to confirm the effectiveness of active wound dressings with cold plasma in the treatment of chronic wounds.


Assuntos
Gases em Plasma , Humanos , Idoso de 80 Anos ou mais , Gases em Plasma/uso terapêutico , Cicatrização , Bandagens , Desbridamento
9.
Ann Afr Med ; 21(4): 451-455, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36412350

RESUMO

Background: In a health-care setup, interpersonal communication plays a pivotal role in the attainment of professional outcomes. Dressing is an indirect mode of communication. Since ancient times, it is assumed that doctor's clothing have a positive effect on the minds of patients. This survey evaluates the perception of interns from dental sciences regarding the impact of dressing on professional performance at workplace. Materials and Methods: A self-designed questionnaire was distributed among a batch of interns who were willing to participate in the survey. Seventy-six interns returned the responses which were complete without any duplication. All the survey forms were scrutinized and the data were collated. Qualitative analysis of the assembled data was performed to evaluate intern's perception about the association between dressing and professional performance at workplace. Results: Results of the survey demonstrated that 81.6% of the participants dress up to work as per their temperament. About 65.8% of the participants believed that dental professionals should choose formal wear for workplace settings. Majority of the participants, i.e., 67.1%, believed that dressing affects perception on personality traits such as dignity and character of a professional. A wide number of interns perceived that dressing alters the behavior of self, patients, and colleagues (55.3%, 71.1%, and 68.4%, respectively). The result of this survey also showed a positive correlation between dressing and professional traits such as confidence, responsibility, and efficiency (75.0%, 63.2%, and 63.2%, respectively). Notably, 55.3% opined against enforcing a dress code for dental institutions/dental offices. Conclusion: The result of the study represents the positive influence of dressing on professional performance at workplace in dentistry as perceived by interns from the faculty of dental sciences.


Résumé Contexte: Dans une configuration de soins de santé, la communication interpersonnelle joue un rôle central dans l'atteinte des résultats professionnels. Pansement est un mode de communication indirect. Depuis l'Antiquité, on suppose que les vêtements de médecin ont un effet positif sur l'esprit des patients. Cette enquête évalue la perception des stagiaires en sciences dentaires concernant l'impact de l'habillage sur la performance professionnelle au travail. Matériels et méthodes: Un questionnaire auto-conçu a été distribué à un groupe de stagiaires disposés à participer auenquête. Soixante-seize stagiaires ont renvoyé les réponses qui étaient complètes sans aucune duplication. Tous les formulaires d'enquête ont été examinés etles données ont été rassemblées. Une analyse qualitative des données assemblées a été effectuée pour évaluer la perception des stagiaires sur l'association entre habillage et performance professionnelle sur le lieu de travail. Résultats: Les résultats de l'enquête ont démontré que 81,6 % des participants s'habillaient travailler selon son tempérament. Environ 65,8% des participants pensaient que les professionnels dentaires devraient choisir des vêtements de cérémonie pour le lieu de travail réglages. La majorité des participants, c'est-à-dire 67,1%, croyaient que l'habillement affecte la perception des traits de personnalité tels que la dignité et le caractère d'un professionnel. Un grand nombre d'internes ont perçu que s'habiller modifie leur comportement, celui des patients et des collègues (55,3 %, 71,1 % et 68,4 %, respectivement). Le résultat de cette enquête a également montré une corrélation positive entre l'habillement et les traits professionnels tels que la confiance, responsabilité et efficacité (75,0 %, 63,2 % et 63,2 %, respectivement). Notamment, 55,3 % se sont prononcés contre l'application d'un code vestimentaire pour les soins dentaires institutions/cabinets dentaires. Conclusion: Le résultat de l'étude représente l'influence positive de l'habillement sur la performance professionnelle à milieu de travail en dentisterie tel que perçu par les stagiaires de la faculté des sciences dentaires. Mots clés: Dentisterie, habillage, interne, performance professionnelle, lieu de travail.


Assuntos
Internato e Residência , Local de Trabalho , Humanos , Percepção , Bandagens , Odontologia
10.
J Wound Care ; 31(11): 898-906, 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36367803

RESUMO

OBJECTIVE: It is theorised that adhesive-free wound care developed specifically for patients with hidradenitis suppurativa (HS) can improve their quality of life (QoL). Our study aimed to investigate the impact of a novel wound care device on Dermatology Life Quality Index (DLQI) scores, and other factors related to experienced pain, time spent changing dressings, comfort, ease of use and body image. METHOD: A 21-day, single-arm, unblinded, pilot trial was conducted to assess ease of use and the impact of effective wound care on various aspects of wound management in patients with HS. Participants were provided two trial garments and trial dressings as required, to use over a 21-day period in the home setting. A seven-item questionnaire and the DLQI questionnaire was completed on days 0, 7, 14 and 21. RESULTS: All 15 participants were female, aged >18 years old and with a diagnosis of HS. Mean DLQI score at baseline (day 0) was 19.3, which was reduced to 4.53 on day 21, a significant improvement in 100% of participants (p<0.001). High levels of dressing-related pain, assessed using an 11-point Visual Analogue Scale, reduced from 5.53 at baseline to 0.8 on day 21. Other significant improvements in terms of patient comfort, time spent on changing dressings, body confidence and the dressing's ability to retain exudate were also noted. CONCLUSION: The results illustrated the improvement made to study participants' day-to-day activities and QoL when effective HS-specific wound care products were provided. Wound care is an essential component in the treatment journey of patients.


Assuntos
Hidradenite Supurativa , Humanos , Feminino , Adolescente , Masculino , Hidradenite Supurativa/terapia , Hidradenite Supurativa/diagnóstico , Qualidade de Vida , Projetos Piloto , Bandagens , Dor/etiologia
11.
J Neuroeng Rehabil ; 19(1): 117, 2022 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329473

RESUMO

BACKGROUND: Service robots are defined as reprogrammable, sensor-based mechatronic devices that perform useful services in an autonomous or semi-autonomous way to human activities in an everyday environment. As the number of elderly people grows, service robots, which can operate complex tasks like dressing tasks for disabled people, are being demanded increasingly. Consequently, there is a growing interest in studying dressing tasks, such as putting on a t-shirt, a hat, or shoes. Service robots or robot manipulators have been developed to accomplish these tasks using several control approaches. The robots used in this kind of application are usually bimanual manipulator (i.e. Baxter robot) or single manipulators (i.e. Ur5 robot). These arms are usually used for recognizing clothes and then folding them or putting an item on the arm or on the head of a person. METHODS: This work provides a comprehensive review of the most relevant attempts/works of robotic dressing assistance with a focus on the control methodology used for dressing tasks. Three main areas of control methods for dressing tasks are proposed: Supervised Learning (SL), Learning from Demonstration (LfD), and Reinforcement Learning (RL). There are also other methods that cannot be classified into these three areas and hence they have been placed in the other methods section. This research was conducted within three databases: Scopus, Web of Science, and Google Scholar. Accurate exclusion criteria were applied to screen the 2594 articles found (at the end 39 articles were selected). For each work, an evaluation of the model is made. CONCLUSION: Current research in cloth manipulation and dressing assistance focuses on learning-based robot control approach. Inferring the cloth state is integral to learning the manipulation and current research uses principles of Computer Vision to address the issue. This makes the larger problem of control robot based on learning data-intensive; therefore, a pressing need for standardized datasets representing different cloth shapes, types, materials, and human demonstrations (for LfD) exists. Simultaneously, efficient simulation capabilities, which closely model the deformation of clothes, are required to bridge the reality gap between the real-world and virtual environments for deploying the RL trial and error paradigm. Such powerful simulators are also vital to collect valuable data to train SL and LfD algorithms that will help reduce human workload.


Assuntos
Pessoas com Deficiência , Robótica , Humanos , Idoso , Algoritmos , Simulação por Computador , Bandagens
12.
Biomater Adv ; 143: 213163, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36327826

RESUMO

Any interference in the timely and orderly progression through all the phases of healing process can turn a minor injury into a chronic wound. Most of the wound dressings available in the market are moderately effective and have not shown satisfactory improvement in healing. Along with the appropriate wound management, it is imperative for a dressing to facilitate the wound repair process too. In the present research, we hypothesize to improve the wound healing process by applying cost effective natural cocktail of various bioactive factors. Bovine colostrum contains high levels of immunoglobulins, lactoferrin, hormones and cytokines which play significant role in wound healing. Hence, multifunctional colostrum conjugated PCL-PEG based nanofibrous dressings were developed and analyzed for their physicochemical properties and cellular responses. The dressings were also evaluated for cell migration, antioxidant, anti-inflammatory and anti-bacterial properties. In-vivo wound healing ability was validated on a rat wound model. Numerous growth factors present in the colostrum showed their role in stimulation of skin repair and regeneration by direct action on genetic material. Significantly less inflammation in colostrum treated wounds was observed due to anti-inflammatory properties of lactoferrin. Thus obtained results confirmed the suitability of these multifunctional colostrum conjugated nanofibrous dressings for improved wound healing.


Assuntos
Nanofibras , Animais , Bovinos , Ratos , Nanofibras/uso terapêutico , Lactoferrina/farmacologia , Bandagens , Cicatrização , Peptídeos e Proteínas de Sinalização Intercelular/farmacologia
13.
Biomater Adv ; 143: 213172, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36343392

RESUMO

Deep skin wound needs a long wound healing process, in which external force on skin around wound can result in a sharp pain, wound re-damage and interstitial fluid flowing out, increasing the risk of deterioration and even amputation. While the conventional wound dressings cannot provide timely feedback of abnormal wound status and lose best time for wound treatment, real-time monitoring wound status is thus urgently needed for wound management. In this work, a breathable and stretchable electronic skin (i.e., e-skin) named CNTs/graphene/GelMA mat has been developed through electrospinning, ice-templating and in-situ loading method for evaluating wound status. The obtained porosity, swelling ratio and vapor transmission rate of the CNTs/graphene/GelMA mat are 55 %, 180 % and 3378.2 h-1 day-1, respectively. And owing to the good porous, nanofibrous architecture and excellent breathability of the mat, L929 cells grow and well spread on the CNTs/graphene/GelMA mat. In addition, the gauge factors of the prepared conductive CNTs/graphene/GelMA mat as a strain sensor are 15.4 and 72.9 in the strain ranges of 0-70 % and 70-85 %, respectively, matching the mechanical performance of human skin. The sensitivity coefficient of the mat for moisture sensing is 12.05, indicating its high efficiency for monitoring and warning interstitial fluid outflow from wound. Furthermore, the integration of CNTs/graphene/GelMA mat with a portable device is feasible to monitor strain and moisture on a rat model with abdominal wound. The healing process of the wounds treated with CNTs/graphene/GelMA mat is similar to that of GelMA mat, indicating that the dosage of CNTs and graphene in the CNTs/graphene/GelMA mat has negligible effect on the mat histocompatibility. The CNTs/graphene/GelMA mat demonstrates the application potential in wound management, home medical diagnosis and human-machine interactions.


Assuntos
Grafite , Nanofibras , Humanos , Ratos , Animais , Grafite/farmacologia , Cicatrização , Bandagens , Condutividade Elétrica
14.
Medicina (Kaunas) ; 58(11)2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36363504

RESUMO

Background and Objectives: Scar formation after burn trauma has a significant impact on the quality of life of burn patients. Hypertrophic scars or keloids can be very distressing to patients due to potential pain, functional limitations, or hyper- or hypopigmentation. In a previous study comparing Suprathel® and the new and cheaper dressing epicitehydro®, we were able to show that pain reduction, exudation, and time until wound-healing of partial-thickness burn wounds were similar, without any documented infections. No study exists that objectively measures and compares skin and scar quality after treatment with Suprathel® and epicitehydro® at present. Materials and Methods: In this study, the scar quality of 20 patients who had been treated with Suprathel® and epicitehydro® was objectively assessed using the Cutometer®, Mexameter®, and Tewameter®, as well as subjectively with the Patient and Observer Scar Assessment Scale, 3, 6, and 12 months after burn injury. Results: In all performed measurements, no significant differences were detected in scar formation after treatment of partial-thickness burn wounds with the two dressings. Conclusions: Both the newer and less expensive wound-dressing epicitehydro® and the well-known wound-dressing Suprathel® resulted in stable wound closure and showed good cosmetic results in the follow-up examinations.


Assuntos
Queimaduras , Cicatriz , Humanos , Cicatriz/etiologia , Transplante de Pele/métodos , Qualidade de Vida , Queimaduras/complicações , Queimaduras/terapia , Bandagens , Dor
15.
Int J Mol Sci ; 23(22)2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36430295

RESUMO

In this study, a hydrogel composite wound dressing with antibacterial and self-healing ability was prepared using cysteine-modified carboxymethyl chitosan, sodium oxidized alginate, and but-3-yn-2-one base on Schiff base and thiol-alkynone double cross-links. The structure and properties of the hydrogel were characterized by scanning electron microscope, Fourier-transform infrared, and rheological test, followed by antibacterial and in vivo biocompatibility tests. The results showed that the hydrogel exhibited good self-healing, mechanical properties, good antibacterial effect, and in vivo biocompatibility, and can inhibit inflammation and promote skin tissue regeneration in mice. This novel self-healing hydrogel dressing has a broad application prospect in skin tissue engineering.


Assuntos
Surdez , Prunella , Animais , Camundongos , Hidrogéis/farmacologia , Bases de Schiff , Compostos de Sulfidrila , Bandagens , Polissacarídeos/farmacologia , Carboidratos da Dieta , Antibacterianos/farmacologia
16.
Molecules ; 27(22)2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36431858

RESUMO

Wounds represent a major global health challenge. Acute and chronic wounds are sensitive to bacterial infection. The wound environment facilitates the development of microbial biofilms, delays healing, and promotes chronic inflammation processes. The aim of the present work is the development of chitosan films embedded with bud poplar extract (BPE) to be used as wound dressing for avoiding biofilm formation and healing delay. Chitosan is a polymer with antimicrobial and hydrating properties used in wound dressing, while BPE has antibacterial, antioxidative, and anti-inflammatory properties. Chitosan-BPE films showed good antimicrobial and antibiofilm properties against Gram-positive bacteria and the yeast Candida albicans. BPE extract induced an immunomodulatory effect on human macrophages, increasing CD36 expression and TGFß production during M1/M2 polarization, as observed by means of cytofluorimetric analysis and ELISA assay. Significant antioxidant activity was revealed in a cell-free test and in a human neutrophil assay. Moreover, the chitosan-BPE films induced a good regenerative effect in human fibroblasts by in vitro cell migration assay. Our results suggest that chitosan-BPE films could be considered a valid plant-based antimicrobial material for advanced dressings focused on the acceleration of wound repair.


Assuntos
Anti-Infecciosos , Quitosana , Humanos , Quitosana/farmacologia , Quitosana/metabolismo , Bandagens/microbiologia , Cicatrização , Anti-Infecciosos/farmacologia , Antibacterianos/farmacologia , Extratos Vegetais/farmacologia
17.
Biomater Adv ; 143: 213175, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36368057

RESUMO

Skin wounds have been reported to increase the number of microbial colonies susceptible to infection. Treatments using oral antibiotics have been limited due to their toxicity and hydrophobic characteristics. In this study, we developed a formulation of chloramphenicol microparticles (CPL MPs), which was modified into chitosan hydrogel to increase treatment efficiency in targeting infections and creating an optimal environment to support the healing process. CPL MPs were prepared by a cross-linker stabilized method using whey protein (WPI) biopolymer, and the CPL MPs hydrogel was designed using chitosan biopolymer. Based on the result, CPL-loaded MPs showed desired physical and encapsulation characteristics. In the in vitro study, drug release of CPL MPs in simulated wound fluid represented approximately 99.40 ± 7.01 % of the system after 24 h. The antibacterial activity of CPL-loaded MPs formulation (MIC value 12.5 µg/mL, MBC 25 µg/mL) was effective as MIC concentration increased. Furthermore, the formulation of CPL MPs into hydrogel showed a better dermatokinetic profile compared to hydrogel with pure CPL. Interestingly, the antibacterial activity of the ex vivo infection model showed that Staphylococcus aureus activity decreased by up to 99.98 % after 24 h administration of CPL MPs hydrogel when compared to pure-CPL hydrogel and blank hydrogel. These studies have confirmed that incorporating CPL MPs into hydrogel can provide a promising approach to skin infection treatment.


Assuntos
Quitosana , Quitosana/química , Hidrogéis/química , Bandagens , Antibacterianos/farmacologia , Cloranfenicol/farmacologia
18.
Biomater Adv ; 143: 213177, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36371970

RESUMO

Nanotechnologies are being increasingly applied as systems for peptide and nucleic acid macromolecule drug delivery. However systemic targeting of these, or efficient topical and localized delivery remains an issue. A controlled release system that can be patterned and locally administered such as topically to accessible tissue (skin, eye, intestine) would therefore be transformative in realizing the potential of such strategies. We previously developed a technology termed GAG-binding enhanced transduction (GET) to efficiently deliver a variety of cargoes intracellularly, using GAG-binding peptides to mediate cell targeting, and cell penetrating peptides (CPPs) to promote uptake. Herein we demonstrate that the GET transfection system can be used with the moisturizing thermo-reversible hydrogel Pluronic-F127 (PF127) and methyl cellulose (MC) to mediate site specific and effective intracellular transduction and gene delivery through GET nanoparticles (NPs). We investigated hydrogel formulation and the temperature dependence of delivery, optimizing the delivery system. GET-NPs retain their activity to enhance gene transfer within our formulations, with uptake transferred to cells in direct contact with the therapy-laden hydrogel. By using Azowipe™ material in a bandage approach, we were able to show for the first-time localized gene transfer in vitro on cell monolayers. The ability to simply control localization of gene delivery on millimetre scales using contact-mediated transfer from moisture-providing thermo-reversible hydrogels will facilitate new drug delivery methods. Importantly our technology to site-specifically deliver the activity of novel nanotechnologies and gene therapeutics could be transformative for future regenerative medicine.


Assuntos
Peptídeos Penetradores de Células , Hidrogéis , Hidrogéis/química , Poloxâmero/química , Bandagens , Terapia Genética
19.
Khirurgiia (Mosk) ; (11): 42-48, 2022.
Artigo em Russo | MEDLINE | ID: mdl-36398954

RESUMO

Purulent-inflammatory lesions of skin and soft tissues account for 30-35% of causes of admission to surgical hospitals. In outpatient system, this value reaches 35-60%. Moreover, suppuration of wounds after elective surgery occurs in 2-5% of cases. Adequately selected drugs for local wound therapy can achieve localization of purulent process and avoid spread of infection. This review summarizes data on development, experimental and clinical testing of wound dressings over the past 10 years, presents classification options and analyzes their advantages and disadvantages. The authors emphasize higher efficacy of hydrophilic coatings, and coatings based on chitosan, collagen, methylcellulose and its derivatives are preferred. It is advisable to introduce a component with antimicrobial, anesthetic and hemostatic activity stimulating tissue regeneration into the matrix of wound dressing. The priority is introduction of antiseptic substances causing fewer side effects compared to antibiotics. Further research and development of new wound dressings is a priority in surgery considering changing landscape and development of resistance of microorganisms.


Assuntos
Anti-Infecciosos Locais , Bandagens , Humanos , Cicatrização , Anti-Infecciosos Locais/farmacologia , Pele/patologia , Colágeno , Antibacterianos/farmacologia
20.
Ceska Slov Farm ; 71(5): 190-199, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36443024

RESUMO

Films are thin, flexible, and transparent wound dressings. They can be prepared from both synthetic and natural materials. In practice, synthetic polyurethane dominates, but research is mainly focused on substances of natural origin. An endogenous substance with excellent filmforming properties, which is involved in the wound healing process, is collagen. However, collagen films themselves have weak mechanical properties, which can be improved by, among other things, combining collagen with other materials. Such material could be carboxymethylcellulose, which has been shown to affect wound healing positively. Films consisting only of CMC also have weak mechanical properties, so combining both materials seems to be a suitable solution to the given problems, and a wound dressing with many beneficial properties for wound healing could be created. Therefore, our experiment aimed to prepare composite films for wound therapy consisting of a combination of collagen and CMC. The films were prepared by the solvent evaporation method, and their properties were compared with those formed only by CMC. In both cases, films with suitable organoleptic, physicochemical, and application properties for wound therapy were produced. The composite films showed lower absorption capacity and better mechanical resistance compared to those formed only by CMC. The combination of collagen and CMC in composite films intended for wound therapy has thus resulted in improved properties of the resulting dressing and holds potential for further research.


Assuntos
Bandagens , Carboximetilcelulose Sódica , Colágeno , Cicatrização , Poliuretanos
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