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1.
Ann Palliat Med ; 10(9): 9443-9452, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34628870

RESUMO

BACKGROUND: Postoperative delirium (POD) and postoperative cognitive dysfunction (POCD) are the most common central nervous system dysfunctions during the perioperative period. Melatonin protects nerve cells and impacts cognitive functioning in patients after surgery. METHODS: A total of 120 patients undergoing elective non-cardiac surgery were evaluated with the confusion assessment method (CAM) for diagnosis of POD on the day before and the 1st, 2nd, 3rd, and 7th day after surgery. Also, a neuropsychological test for the diagnosis of POCD was performed on the day before and 1 week after surgery. Patients' urine was collected to examine the concentration of 6-sulfatoxymelatonin (6-SMT), the metabolite of melatonin, with the enzyme-linked immunosorbent assay method. Meanwhile, urine creatinine values were examined to calculate the 6-SMT/creatinine ratio (M/C). RESULTS: The incidence rates of POD and POCD were 7% and 44%, respectively. There were no statistically differences for the M/C on the 1st, 2nd, 3rd, and 7th day after surgery between the POD and the non-POD groups (P>0.05). However, there were statistically significant differences (P<0.05) in the rates of M/C change [(preoperative value-postoperative value)/(postoperative value) ×100%] on the 1st and 7th day after surgery between both groups. Patients were divided into Group I1 (≥100%) and Group II1 (<100%) based on the M/C rate changes on the 1st day, Group I7 (≥200%) and Group II7 (<200%) based on the M/C rate changes on the 7th day, and Group Iw (≥100%) and Group IIw (<100%) based on the M/C rate changes during the 1st week after surgery. The incidence rates of POD for Group I1 and Group II1 were 21.1% and 3.7%, respectively; for Group I7 and Group II7 were 50% and 1.1%, respectively; for Group Iw and Group IIw were 17.2% and 2.8%, respectively. For 7 patients with POD had POCD, the occurrence of POCD was related to POD (P<0.05). CONCLUSIONS: Increased melatonin after surgery may be a risk factor for POD. There may be no correlation between melatonin and POCD. POD may be a risk factor of POCD.


Assuntos
Transtornos Cognitivos , Delírio , Melatonina , Complicações Cognitivas Pós-Operatórias , Humanos , Incidência
2.
J Contemp Dent Pract ; 22(7): 739-744, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34615777

RESUMO

AIM AND OBJECTIVE: To histologically compare alveolar bone repair after tooth extraction treated with melatonin and calcium sulfate in an in vivo experimental study in guinea pigs (Cavia porcellus). MATERIALS AND METHODS: The study was of longitudinal, prospective, and experimental design in an animal bio-model. A total of 24 male guinea pigs were included, weighing from 700 to 900 g and separated into two experimental groups (melatonin and calcium sulfate) for three periods (15, 30, and 45 days) at 15-day intervals after surgery. The guinea pigs were randomly included into groups for the time evaluated. RESULTS: In relation to bone repair cells using calcium sulfate, the presence of osteoblasts at 15, 30, and 45 days was 39.0 ± 63, 55.3 ± 6.0, respectively, with 61.3 ± 10.0 cells per field. Regarding bone repair cells using melatonin, the presence of osteoblasts at 15, 30, and 45 days was 25.0 ± 3.7, 49.3 ± 1.5, respectively, with 53.6 ± 5.6 cells per field. CONCLUSION: Both melatonin and calcium sulfate were found to be useful in bone repair at a histological and clinical level, although they present certain nonsignificant, albeit marked advantages in the bone repair process when compared with the control socket at the histological level. CLINICAL SIGNIFICANCE: This research allows us to know the usefulness of these easily accessible chemicals for the generation of bone repair.


Assuntos
Sulfato de Cálcio , Melatonina , Animais , Cobaias , Masculino , Melatonina/farmacologia , Estudos Prospectivos , Projetos de Pesquisa
3.
Ecotoxicol Environ Saf ; 226: 112816, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34597844

RESUMO

Cold stress is an adverse environmental condition that limits the growth and yield of leguminous plants. Thus, discovering an effective way of ameliorating cold-mediated damage is important for sustainable legume production. In this study, the combined use of Rhizobium inoculation (RI) and melatonin (MT) pretreatment was investigated in Medicago truncatula plants under cold stress. Eight-week-old seedlings were divided into eight groups: (i) CK (no stress, noninoculated, no MT), (ii) RI (Rhizobium inoculated), (iii) MT (75 µM melatonin), (iv) RI+MT, (v) CS (cold stress at 4 °C without Rhizobium inoculation and melatonin), (vi) CS+RI, (vii) CS+MT, and (viii) CS+RI+MT. Plants were exposed to cold stress for 24 hrs. Cold stress decreased photosynthetic pigments and increased oxidative stress. Pretreatment with RI and MT alone or combined significantly improved root activity and plant biomass production under cold stress. Interestingly, chlorophyll contents increased by 242.81% and MDA levels dramatically decreased by 34.22% in the CS+RI+MT treatment compared to the CS treatment. Moreover, RI+MT pretreatment improved the antioxidative ability by increasing the activities of peroxidase (POD; 8.45%), superoxide dismutase (SOD; 50.36%), catalase (CAT; 140.26%), and ascorbate peroxidase (APX; 42.63%) over CS treated plants. Additionally, increased osmolyte accumulation, nutrient uptake, and nitrate reductase activity due to the combined use of RI and MT helped the plants counteract cold-mediated damage by strengthening the nonenzymatic antioxidant system. These data indicate that pretreatment with a combined application of RI and MT can attenuate cold damage by enhancing the antioxidation ability of legumes.


Assuntos
Medicago truncatula , Melatonina , Rhizobium , Antioxidantes , Resposta ao Choque Frio , Melatonina/farmacologia , Estresse Oxidativo , Plântula
4.
Ecotoxicol Environ Saf ; 226: 112878, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34634736

RESUMO

Herein, we further document the protective action of melatonin (MLT) in mitigating cadmium (Cd) effects on adult rat testis. Cd treatment provoked testicular injury, that was documented by histological and biomolecular alterations, i.e., decrease of serum and testicular testosterone concentration and modified sperm parameters. Mainly, both the cytoarchitecture of the blood-testis barrier (BTB) and germ cell morphology were perturbed, as highlighted by impairment in structural (OCN, VANGL, Cx43) and regulative (Src and FAK) protein levels and/or activation. The study focused on the involvement of the autophagy pathway, that was enhanced especially in the Sertoli cells, probably in response to the disorganization of the BTB. Results obtained with the MLT co-treatment demonstrated that its administration decreased the level of oxidative damage caused by Cd, with reversal of all the observed changes. Moreover, the beneficial effects of MLT alone were evidenced by an increase of sperm quality, in term of motility and DNA integrity. The combined results, obtained in rat, strongly encourage to consider a role for MLT in improving also human testicular health, not only in men exposed to Cd, but also in those having fertility disorders, to ameliorate sperm quality and, consequently, reproductive success.


Assuntos
Barreira Hematotesticular , Melatonina , Animais , Cádmio/toxicidade , Masculino , Melatonina/farmacologia , Ratos , Espermatozoides , Testículo
5.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(9): 1409-1414, 2021 Aug 31.
Artigo em Chinês | MEDLINE | ID: mdl-34658357

RESUMO

OBJECTIVE: To explore the effect of melatonin (MT) on 2, 2', 4, 4'-tetrabromodiphenylether (PBDE-47)-induced abnormal autophagy and apoptosis in rat adrenal medullary pheochromocytoma PC12 cells. METHODS: PC12 cells were pretreated with a concentration gradient (12.5, 25, 50, 100, and 200 µmol/L) of melatonin for 2 h before exposure to 20 µmol/L PBDE-47 for 24 h to determine the optimal concentration of melatonin for cell treatment. In subsequent experiments, PC12 cells were treated with 0.5‰ DMSO (control group), 20 µmol/L PBDE-47, 25 µmol/L melatonin, or both PBDE-47 and melatonin. Immunofluorescence assay was used to detect the positive staining of microtubule associated protein 1 light chain 3 (LC3; a marker protein of autophagy); Western blotting was performed to determine the expression levels of the key autophagic proteins including autophagy-related protein 7 (ATG7), LC3-Ⅱ and autophagy substrate p62, and the key apoptotic proteins including active cysteine-containing aspartate specific protease-3 (active caspase-3) and cleaved poly(ADP ribose) polymerase (cleaved PARP). RESULTS: PBDE-47 treatment significantly reduced the viability of PC12 cells (P=0.001), but pretreatment with 25 µmol/L melatonin maintained a cell viability over 80% following exposure to PBDE-47 (P=0.023). PBDE-47-treated PC12 cells showed obviously enhanced immunofluorescent staining of LC3 protein, a significantly decreased expression of ATG7 and increased expression levels of p62, LC3-Ⅱ, active caspase-3 and cleaved PARP (P < 0.001). The cells treated with both PBDE-47 and melatonin showed obviously reduced staining of LC3 protein with a signficantly increased expression level of ATG7 (P=0.034) and decreased expressions of p62 (P=0.048), LC3-Ⅱ (P=0.018), active caspase-3 (P < 0.001) and cleaved PARP (P=0.032). CONCLUSION: PBDE-47 exposure impairs autophagy to cause autophagosome accumulation and promote apoptosis of PC12 cells. Melatonin can improve PBDE-47-induced abnormal autophagy and apoptosis and thus promote the survival of PC12 cells.


Assuntos
Melatonina , Animais , Apoptose , Autofagia , Éteres Difenil Halogenados , Melatonina/farmacologia , Células PC12 , Ratos
6.
Arq Neuropsiquiatr ; 79(8): 732-742, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34550191

RESUMO

BACKGROUND: Insomnia is a sleep disorder characterized by difficulty of falling asleep or maintaining sleep, which affects different age groups. Currently, melatonin is used as a therapeutic treatment in cases of insomnia in children, adults, and elderly people. OBJECTIVE: To evaluate the effectiveness of melatonin in sleep disorders, its dosage, potential adverse effects, as well as labeling laws and regulations in Brazil. METHODS: This integrative review was carried out using the Cochrane Library, Medline (Pubmed), and Science Direct databases. Twenty-five articles and three documents available on the Brazilian Society of Endocrinology and Metabology (SBEM) and National Health Surveillance Agency (ANVISA) websites published between 2015 and 2020 were selected to be evaluated in full. RESULTS: It was found that in most of the selected articles the use of melatonin reduces sleep latency. The effective melatonin doses varied according to each age group, from 0.5 to 3 mg in children, 3 to 5 mg in adolescents, 1 to 5 mg in adults, and 1 to 6 mg in elderly people. Side effects are mild when taking usual doses. In Brazil, no registered drug and current regulation on the use and marketing of melatonin has been identified. CONCLUSION: The use of melatonin is an alternative therapy that can be used for sleeping disorders. According to the evidences found, it did not demonstrate toxicity or severe side effects, nor dependence even when administered at high doses, suggesting that it is a safe medication to treat patients of different ages suffering from sleeping disorders.


Assuntos
Melatonina , Distúrbios do Início e da Manutenção do Sono , Transtornos do Sono-Vigília , Adolescente , Adulto , Idoso , Brasil , Criança , Humanos , Melatonina/uso terapêutico , Sono , Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico , Transtornos do Sono-Vigília/tratamento farmacológico
7.
Ecotoxicol Environ Saf ; 225: 112783, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34544023

RESUMO

Sudan I is one of the industry dyes and widely used in cosmetics, wax agent, solvent and textile. Sudan I has multiple toxicity such as carcinogenicity, mutagenicity, genotoxicity and oxidative damage. However, Sudan I has been illegally used as colorant in food products, triggering worldwide attention about food safety. Nevertheless, the toxicity of Sudan I on reproduction, particularly on oocyte maturation is still unclear. In the present study, using mouse in vivo models, we report the toxicity effects of Sudan I on mouse oocyte. The results reflect that Sudan I exposure disrupts spindle organization and chromosomes alignment as well as cortical actin distribution, thus leading to the failure of polar body extrusion. Based on the transcriptome results, it is found that the exposure of Sudan I leads to the change in expression of 764 genes. Moreover, it's further reflected that the damaging effects of Sudan I are mediated by the destruction of mitochondrial functions, which induces the accumulated ROS to stimulate oxidative stress-induced apoptosis. As an endogenous hormone, melatonin within the ovarian follicle plays function on improving oocyte quality and female reproduction by efficiently suppressing oxidative stress. Moreover, melatonin supplementation also improves oocyte quality and increases fertilization rate during in vitro culture. Consistent with these, we find that in vivo supplementation of melatonin efficaciously suppresses mitochondrial dysfunction and the accompanying apoptosis, thus reverses oocyte meiotic deteriorations. Collectively, our results prove the reproduction toxicity of Sudan I for the exposure of Sudan I reduces the oocyte quality, and demonstrate the protective effects of melatonin against Sudan I-induced meiotic deteriorations.


Assuntos
Melatonina , Animais , Apoptose , Feminino , Meiose , Melatonina/metabolismo , Melatonina/farmacologia , Camundongos , Mitocôndrias , Naftóis , Oócitos/metabolismo , Estresse Oxidativo
8.
Iran J Kidney Dis ; 15(5): 351-363, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34582370

RESUMO

INTRODUCTION: Circadian system is deeply involved in renal function. The circadian timing system may be disrupted in chronic kidney disease (CKD) patients. Gender differences in CKD have been reported. This research aimed to investigate the gender differences in the circadian rhythm of inflammatory and oxidant markers of CKD. METHODS: Male, intact female, and ovariectomized (OVX) female rats (twenty-four in each group) were randomly assigned to control and CKD groups. The rats were further divided into day (12:00 p.m.) and night (12:00 a.m.) subgroups. Evaluations of each sample were carried out a day after the last day of adenine administration. RESULTS: Final results revealed that the circadian rhythm of plasma melatonin , kidney malondialdehyde (MDA), and transforming growth factor- ß (TGF-ß) levels in CKD group were the same as the control group. Melatonin and total antioxidant capacity (TAC) levels significantly decreased in the CKD group compared with the control group in day and night subgroups, whereas MDA and TGF-ß levels increased. Male group in comparison with the intact female group significantly showed less melatonin and TAC but higher MDA and TGF-ß levels which could be due to CKD. CONCLUSION: Findings of this study represent gender differences in circadian rhythm amplitude of inflammation, melatonin, and oxidative stress in CKD animals, probably in favor of female sex steroids. These findings emphasize on the importance of gender differences in CKD progression; therefore, considerable attention must be paid to gender in the treatment of CKD. INTRODUCTION: Circadian system is deeply involved in renal function. The circadian timing system may be disrupted in chronic kidney disease (CKD) patients. Gender differences in CKD have been reported. This research aimed to investigate the gender differences in the circadian rhythm of inflammatory and oxidant markers of CKD. METHODS: Male, intact female, and ovariectomized (OVX) female rats (twenty-four in each group) were randomly assigned to control and CKD groups. The rats were further divided into day (12:00 p.m.) and night (12:00 a.m.) subgroups. Evaluations of each sample were carried out a day after the last day of adenine administration. RESULTS: Final results revealed that the circadian rhythm of plasma melatonin , kidney malondialdehyde (MDA), and transforming growth factor- ß (TGF-ß) levels in CKD group were the same as the control group. Melatonin and total antioxidant capacity (TAC) levels significantly decreased in the CKD group compared with the control group in day and night subgroups, whereas MDA and TGF-ß levels increased. Male group in comparison with the intact female group significantly showed less melatonin and TAC but higher MDA and TGF-ß levels which could be due to CKD. CONCLUSION: Findings of this study represent gender differences in circadian rhythm amplitude of inflammation, melatonin, and oxidative stress in CKD animals, probably in favor of female sex steroids. These findings emphasize on the importance of gender differences in CKD progression; therefore, considerable attention must be paid to gender in the treatment of CKD. DOI: 10.52547/ijkd.6242.


Assuntos
Melatonina , Insuficiência Renal Crônica , Animais , Antioxidantes , Ritmo Circadiano , Feminino , Humanos , Masculino , Estresse Oxidativo , Ratos , Insuficiência Renal Crônica/diagnóstico
9.
Int J Mol Sci ; 22(17)2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34502344

RESUMO

Osteoblasts and osteoclasts are major cellular components in the bone microenvironment and they play a key role in the bone turnover cycle. Many risk factors interfere with this cycle and contribute to bone-wasting diseases that progressively destroy bone and markedly reduce quality of life. Melatonin (N-acetyl-5-methoxy-tryptamine) has demonstrated intriguing therapeutic potential in the bone microenvironment, with reported effects that include the regulation of bone metabolism, acceleration of osteoblastogenesis, inhibition of osteoclastogenesis and the induction of apoptosis in mature osteoclasts, as well as the suppression of osteolytic bone metastasis. This review aims to shed light on molecular and clinical evidence that points to possibilities of melatonin for the treatment of both osteoporosis and osteolytic bone metastasis. It appears that the therapeutic qualities of melatonin supplementation may enable existing antiresorptive osteoporotic drugs to treat osteolytic metastasis.


Assuntos
Antioxidantes/farmacologia , Neoplasias Ósseas/prevenção & controle , Melatonina/farmacologia , Osteoclastos/efeitos dos fármacos , Osteogênese , Osteoporose/prevenção & controle , Animais , Neoplasias Ósseas/secundário , Humanos , Osteoclastos/citologia , Osteoporose/patologia
10.
Plant Physiol Biochem ; 167: 958-969, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34571389

RESUMO

Melatonin is a pleiotropic regulatory molecule in plants and is involved in regulating plant tolerance to drought stress. Here, we conducted transcriptomic and physiological analyses to identify metabolic processes associated with the enhanced tolerance of the melatonin-treated maize (Zea mays L.) seedlings to water deficit. Maize seedlings were foliar sprayed with either 50 µM melatonin or water and exposed to drought stress for 12 d in growth chambers. Drought stress significantly suppressed seedling growth, and melatonin application partially alleviated this growth inhibition. RNA-Seq analysis revealed that genes whose expression was significantly altered by melatonin were mainly related to carbon (C) and nitrogen (N) metabolism. Analysis of transcriptomics, enzyme activity, and metabolite content data, melatonin-treated plants exhibited a higher level of relatively stable C and N metabolism than untreated plants; this phenotype of melatonin-treated plants was associated with their higher photosynthesis, sucrose biosynthesis, N assimilation, and protein biosynthesis capacities under drought stress. Overall, our results suggest that melatonin enhances drought stress tolerance in maize through coordinated regulation of C and N metabolism.


Assuntos
Secas , Melatonina , Carbono/metabolismo , Regulação da Expressão Gênica de Plantas , Melatonina/farmacologia , Nitrogênio/metabolismo , Plântula/metabolismo , Estresse Fisiológico , Zea mays/metabolismo
11.
BMJ Open ; 11(9): e047858, 2021 09 06.
Artigo em Inglês | MEDLINE | ID: mdl-34489275

RESUMO

INTRODUCTION: The circadian rhythm of melatonin secretion is disturbed after general anaesthesia, leading to postoperative sleep disturbance. Small studies investigating the preventive effect of melatonin administration on postoperative sleep disturbance have not reached any conclusions. Therefore, we will conduct a systematic review and meta-analysis to obtain conclusive results. METHODS AND ANALYSIS: We prepared this protocol following the 2015 Preferred Reporting Items for Systematic Reviews and Meta-Analyses for Protocols guidelines. We will conduct a search for randomised controlled trials that evaluated the effect of melatonin and melatonin agonists on postoperative sleep quality in adult patients undergoing general anaesthesia or regional anaesthesia with sedation. We will exclude patients undergoing regional anaesthesia without sedation. Relevant studies will be searched in the following eight databases: MEDLINE, the Cochrane Central Register of Controlled Trials, Embase, Web of Science and four preregistration sites from inception to 1 January 2021. No language restrictions will be applied. Two authors will independently scan and select eligible studies and perform data extraction and assessment of the risk of bias. The Visual Analogue Scale scores for sleep quality will be combined as the mean difference with a 95% CI using a random-effect model; we will use I2 to assess heterogeneity. We will evaluate the quality of trials using the Cochrane methodology and assess the quality of evidence using the Grading of Recommendation Assessment, Development and Evaluation approach. If appropriate, trial sequential analysis will be performed. ETHICS AND DISSEMINATION: No ethical approval is required for this meta-analysis, as it does not include individual patient data. We will disseminate the results of this meta-analysis in a peer-reviewed journal. PROSPERO REGISTRATION NUMBER: CRD42020180167.


Assuntos
Melatonina , Transtornos do Sono-Vigília , Adulto , Humanos , Melatonina/uso terapêutico , Metanálise como Assunto , Sono , Transtornos do Sono-Vigília/prevenção & controle , Revisões Sistemáticas como Assunto
12.
Phys Chem Chem Phys ; 23(36): 20615-20626, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34514491

RESUMO

The accumulation of ß-amyloid (Aß) and tau protein is considered to be an important pathological characteristic of Alzheimer's disease (AD). Failure of medicine targeting Aß has drawn more attention to the influence of tau protein and its fibrillization on neurodegeneration. Increasing evidence shows that melatonin (Mel) can effectively inhibit the formation of tau fibrils and disassemble preformed tau fibrils. However, the underlying mechanism is poorly understood. In this work, we investigated the kinetics of melatonin binding and destabilizing the tetrameric protofilament and octameric filament of tau R3-R4 domains by performing microsecond all-atom molecular dynamics simulations. Our results show that Mel is able to disrupt the C-shaped structure of the tau protofilament and filament, and destabilizes the association between N- and C-termini. Mel predominantly binds to ß1 and ß6-ß8 regions and favors contact with the elongation surface, which is dominantly driven by hydrogen bonding interactions and facilitated by other interactions. The strong π-π stacking interaction of Mel with Y310 impedes the intramolecular CH-π interaction between I308 and Y310, and the cation-π interaction of Mel with R379 interferes with the formation of the D348-R379 salt bridge. Moreover, Mel occupies the protofilament surface in the tetrameric protofilament and prevents the formation of intermolecular hydrogen bonds between residues K331 and Q336 in the octameric filament. Our work provides molecular insights into Mel hindering tau fibrillization or destabilizing the protofilament and filament, and the revealed inhibitory mechanisms provide useful clues for the design of efficient anti-amyloid agents.


Assuntos
Melatonina/química , Simulação de Dinâmica Molecular , Proteínas tau/química , Sítios de Ligação , Humanos
13.
Int J Mol Sci ; 22(17)2021 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-34502152

RESUMO

Major depressive disorder is a disabling disease with the number of affected individuals increasing each year. Current antidepressant treatments take between three to six weeks to be effective with forty percent of patients being resistant to treatment, making it necessary to search for new antidepressant treatments. Ketamine, a phencyclidine hydrochloride derivative, given intravenously, induces a rapid antidepressant effect in humans. In mice, it causes increased neurogenesis and antidepressant-like effects. However, it also produces psychomimetic effects in humans and in rodents increases the locomotor activity. In contrast, melatonin, a hormone secreted by the pineal gland and synthesized in extrapineal sites, increases new neuron formation and causes antidepressant-like effects in adult rodents with no collateral effects. Here, we assessed the effects of a non-effective dose of ketamine in combination with melatonin (KET/MEL), both on neurogenesis as well as on the antidepressant-like effect in mice. Our results showed that KET/MEL combination increased neurogenesis and produced antidepressant-like effects without altering locomotor activity after both single and triple administration protocols. Our data strongly suggest that KET/MEL combination could be used to simultaneously promote neurogenesis, reverting neuronal atrophy and inducing antidepressant-like effects.


Assuntos
Antidepressivos/uso terapêutico , Depressão/tratamento farmacológico , Ketamina/uso terapêutico , Melatonina/uso terapêutico , Animais , Antidepressivos/administração & dosagem , Antidepressivos/farmacologia , Combinação de Medicamentos , Sinergismo Farmacológico , Ketamina/administração & dosagem , Ketamina/farmacologia , Masculino , Melatonina/administração & dosagem , Melatonina/farmacologia , Camundongos , Neurogênese/efeitos dos fármacos
14.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(8): 1165-1170, 2021 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-34549706

RESUMO

OBJECTIVE: To investigate the protective effect of melatonin against myocardial ischemia-reperfusion (IR) injury in mice and the role of Nrf2 signaling in mediating this effect. METHODS: C57/bl6 mice were randomized into sham-operated group(Sham), IR group(IR), IR with melatonin treatment(melatonin+IR)group, and IR with melatonin and Nrf2 inhibitor ML-385 (melatonin+ML-385+IR) group.In the latter 3 groups, mouse models of myocardial IR injury were established by ligation of the left anterior descending coronary artery.The infarct size was measured with Evans blue/TTC staining, and serum LDH level was detected using ELISA.The ejection fraction (EF) and fractional shortening (FS) of the mice were measured using Vevo software.The expressions of Bcl2, Bax, Nrf2, Nrf2 substrates NQO-1 and HO-1, TNF-α, IL-1ß, and IL-6 in the myocardial tissues were detected with Western blotting. RESULTS: Compared with the sham-operated mice, the mouse models of myocardial IR injury showed significantly increased infarct size and serum LDH levels (P < 0.01) with obviously decreased EF and FS (P < 0.01).The mouse models also showed significantly increased expressions of Bax, TNF-α, IL-1ß and IL-6, decreased expression of Bcl2, Nrf2, NQO-1, and HO-1, and increased apoptotic index and TNF-α expression in the myocardial tissue(P < 0.01).Melatonin treatment significantly decreased the infarct size, serum LDH levels, the expressions of Bax, TNF-α, IL-1ß and IL-6(P < 0.01), lowered the apoptotic index, and increased the expressions of Bcl2, Nrf2, NQO-1, and HO-1 in the mouse models(P < 0.01).The effects of melatonin were obviously blocked by ML-385 treatment in the mouse models. CONCLUSION: Melatonin can alleviate myocardial IR injury in mice by inhibiting inflammatory response via activation of Nrf2 signaling.


Assuntos
Melatonina , Traumatismo por Reperfusão Miocárdica , Animais , Melatonina/farmacologia , Melatonina/uso terapêutico , Camundongos , Traumatismo por Reperfusão Miocárdica/tratamento farmacológico , Miocárdio , Fator 2 Relacionado a NF-E2 , Transdução de Sinais
15.
Molecules ; 26(17)2021 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-34500550

RESUMO

Global warming is impacting the growth and development of economically important but sensitive crops, such as soybean (Glycine max L.). Using pleiotropic signaling molecules, melatonin can relieve the negative effects of high temperature by enhancing plant growth and development as well as modulating the defense system against abiotic stresses. However, less is known about how melatonin regulates the phytohormones and polyamines during heat stress. Our results showed that high temperature significantly increased ROS and decreased photosynthesis efficiency in soybean plants. Conversely, pretreatment with melatonin increased plant growth and photosynthetic pigments (chl a and chl b) and reduced oxidative stress via scavenging hydrogen peroxide and superoxide and reducing the MDA and electrolyte leakage contents. The inherent stress defense responses were further strengthened by the enhanced activities of antioxidants and upregulation of the expression of ascorbate-glutathione cycle genes. Melatonin mitigates heat stress by increasing several biochemicals (phenolics, flavonoids, and proline), as well as the endogenous melatonin and polyamines (spermine, spermidine, and putrescine). Furthermore, the positive effects of melatonin treatment also correlated with a reduced abscisic acid content, down-regulation of the gmNCED3, and up-regulation of catabolic genes (CYP707A1 and CYP707A2) during heat stress. Contrarily, an increase in salicylic acid and up-regulated expression of the defense-related gene PAL2 were revealed. In addition, melatonin induced the expression of heat shock protein 90 (gmHsp90) and heat shock transcription factor (gmHsfA2), suggesting promotion of ROS detoxification via the hydrogen peroxide-mediated signaling pathway. In conclusion, exogenous melatonin improves the thermotolerance of soybean plants and enhances plant growth and development by activating antioxidant defense mechanisms, interacting with plant hormones, and reprogramming the biochemical metabolism.


Assuntos
Antioxidantes/metabolismo , Homeostase/efeitos dos fármacos , Melatonina/farmacologia , Oxirredução/efeitos dos fármacos , Fotossíntese/efeitos dos fármacos , Poliaminas/metabolismo , Plântula/efeitos dos fármacos , Soja/efeitos dos fármacos , Termotolerância/efeitos dos fármacos , Ácido Abscísico/metabolismo , Regulação para Baixo/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Glutationa/metabolismo , Resposta ao Choque Térmico/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Reguladores de Crescimento de Plantas/farmacologia , Proteínas de Plantas/metabolismo , Plântula/metabolismo , Transdução de Sinais/efeitos dos fármacos , Soja/metabolismo , Estresse Fisiológico/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacos
16.
Mater Sci Eng C Mater Biol Appl ; 128: 112328, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34474879

RESUMO

Hydrogel-based drug delivery systems have emerged as a promising platform for chronic tissue defects owing to their inherent ability to inhibit pathogenic infection and accelerate rapid tissue regeneration. Here, we fabricated a stable bio-hybrid hydrogel system comprising collagen, aminated xanthan gum, bio-capped silver nanoparticles and melatonin with antimicrobial, antioxidant and anti-inflammatory properties. Highly colloidal bio-capped silver nanoparticles were synthesized using collagen as a reducing cum stabilizing agent for the first time while aminated xanthan gum was synthesized using ethylenediamine treatment on xanthan gum. The synthesized bio-hybrid hydrogel exhibits better gelation, surface morphology, rheology and degelation properties. In vitro assessment of bio-hybrid hydrogel demonstrates excellent bactericidal efficiency against both common wound and multidrug-resistant pathogens and biocompatibility properties. In vivo animal studies demonstrate rapid tissue regeneration, collagen deposition and angiogenesis at the wound site predominantly due to the synergistic effect of silver nanoparticles and melatonin in the hydrogel. This study paves the way for developing biologically functional bio-nano hydrogel systems for promoting effective care for various ailments, including infected chronic wounds.


Assuntos
Melatonina , Nanopartículas Metálicas , Animais , Antibacterianos/farmacologia , Colágeno , Hidrogéis , Melatonina/farmacologia , Prata/farmacologia
17.
Zhen Ci Yan Jiu ; 46(8): 690-4, 2021 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-34472755

RESUMO

OBJECTIVE: To observe the clinical efficacy of Tiaoshen needling (dredging Governor Vessel and regulating mind)in the treatment of chronic insomnia (CI) and its effect on plasma melatonin (MT) and cortisol (CORT) levels, so as to explore its underlying mechanism. METHODS: Sixty patients with CI were randomly divided into the treatment group and the control group, with 30 cases in each group. Both groups were given sleep hygiene education before treatment. Patients in the treatment group received acupuncture or electroacupuncture at Baihui (GV20), Shenting (GV24), Yintang (GV29), bilateral Shenmen (HT7) and Sanyinjiao (SP6). Patients in the control group received acupuncture or electroacupuncture at bilateral Shousanli (LI10), Futu (ST32) and Feiyang(BL58). Both groups were treated every other day, 3 times a week, for a total of 4 weeks. Before and after treatment, Pittsburgh sleep quality index (PSQI) and fatigue severity scale (FSS) were used to evaluate sleep qua-lity and daytime fatigue, the plasma MT and CORT levels were measured by enzyme-linked immunosorbent assay. RESULTS: Compared with before treatment, the PSQI scores and total FSS score of the treatment group decreased significantly (P<0.01,P<0.05), and the plasma MT content increased and CORT decreased significantly in the treatment group (P<0.01),while the sleep-onset time score, sleep disturbance score and the PSQI total score of the control group decreased significantly (P<0.05). Compared with the control group, the PSQI sleep disorder score and total score, and the total FSS score as well as plasma CORT level were significantly down-regulated (P<0.01,P<0.05), while the plasma MT content was up-regulated (P<0.05) in the treatment group. CONCLUSION: Tiaoshen acupuncture can significantly improve the sleep quality of patients with CI and relieve daytime fatigue, which may be related to the increase of plasma MT content in patients with CI, thereby inhibiting the excessive activation of the hypothalamic-pituitary-adrenal axis.


Assuntos
Terapia por Acupuntura , Melatonina , Distúrbios do Início e da Manutenção do Sono , Pontos de Acupuntura , Humanos , Hidrocortisona , Sistema Hipotálamo-Hipofisário , Sistema Hipófise-Suprarrenal , Distúrbios do Início e da Manutenção do Sono/terapia , Resultado do Tratamento
18.
Artigo em Russo | MEDLINE | ID: mdl-34481429

RESUMO

Circadian rhythm of pineal melatonin production is paced by the thalamus suprachiasmatic nucleus (SCN) depending on the lighting conditions via signal transduction to pinealocytes beta-receptors. Melatonin is a natural regulator of many physiological processes, and the decrease of its synthesis leads to various diseases, in particular, insomnia and metabolic disorders. It is known that administration of beta-blockers reduces melatonin production, but the data showing clinical significance of melatonin reduction associated with beta-blockers administration are still contradictory. OBJECTIVE: The influence of long-term administration of beta-blockers to melatonin synthesis, sleep quality and vascular brain damage. MATERIALS AND METHODS: The main study group included 114 patients, aged 47-83, with cardiovascular diseases, who were under a complex therapy with long-term administration of beta-blockers. The comparison group included 110 patients with cardiovascular diseases, similar in age and sex, who did not receive beta-blockers in their complex therapy. The circadian dynamics of melatonin synthesis was observed by excretion of 6-sulfatoxymelatonin (6-SM), the major metabolite of melatonin, in three urinary samples (day, evening, night). All the patients underwent night polysomnography to assess the severity of sleep disorders. The severity of vascular brain damage was assessed using magnetic resonance imaging. RESULTS: The analyses showed large variability in individual values of 6-SM circadian excretion of patients with cardiovascular diseases (from 0.9 to 133 µg/24h with a mid-point 16.8 µg/24h). A considerable decrease of 6-SM circadian excretion is detected in the group of patients taking beta-blockers comparing to those not Me [q 25; q 75]: 12.8 [6.2; 21.1] and 24.0 [12.5; 41.5] µg/24h, respectively (p<0.001), with no differences in sleep values and severity of vascular brain damage. Comparing subgroups of patients with 6-SM circadian excretion lower and higher than 16.8 µg/24h showed a significant increase of sleep latency, decrease of rapid eye movement sleep (REM sleep), increasing number of gliosis foci in white matter of the brain with higher values of leptin, leptin/adiponectin ratio and glycohemoglobin in the group of patients with 6-SM circadian excretion ≤16.8 µg/24h. CONCLUSION: A low level of endogenous melatonin is a risk factor for development of sleep structure and quality disorders, vascular white matter brain damages with a higher risk for metabolic disorders. Long-term beta-blockers administration decrease endogenous melatonin synthesis to 50% increasing the risk for insomnia and vascular brain damage, mostly in patients with lower initial level of 6-SM circadian excretion.: melatonin, 6-sulfatoxymelatonin, beta-blockers, insomnia, vascular white matter brain damage, leptin, adiponectin.


Assuntos
Melatonina , Transtornos do Sono-Vigília , Ritmo Circadiano , Humanos , Sono , Núcleo Supraquiasmático
19.
Nurs Open ; 8(3): 1063-1068, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-34482661

RESUMO

AIM: We aimed to reveal the relationship of meditation with emotional intelligence (EI), sleep quality and melatonin level. DESIGN: A cross-sectional study. METHODS: Our current research was performed on middle-aged women. EI scale, Pittsburgh Sleep Quality Index (PSQI) and night-time saliva melatonin were measured for 65 participants including 33 meditators and 32 controls. RESULTS: The meditation group showed a significantly higher EI score than the control group. In the regression analysis between EI and age, only the meditation group showed a significant positive correlation. The Pearson correlation analysis among all participants revealed a significant negative correlation between PSQI and EI. There was no significant group difference in the melatonin and PSQI.


Assuntos
Meditação , Melatonina , Estudos Transversais , Inteligência Emocional , Feminino , Humanos , Pessoa de Meia-Idade , Saliva
20.
Int J Mol Sci ; 22(16)2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34445329

RESUMO

Melatonin is registered to treat circadian rhythm sleep-wake disorders and insomnia in patients aged 55 years and over. The essential role of the circadian sleep rhythm in the deterioration of sleep quality during COVID-19 confinement and the lack of an adverse effect of melatonin on respiratory drive indicate that melatonin has the potential to be a recommended treatment for sleep disturbances related to COVID-19. This review article describes the effects of melatonin additional to its sleep-related effects, which make this drug an attractive therapeutic option for treating patients with COVID-19. The preclinical data suggest that melatonin may inhibit COVID-19 progression. It may lower the risk of the entrance of the SARS-CoV-2 virus into cells, reduce uncontrolled hyper-inflammation and the activation of immune cells, limit the damage of tissues and multiorgan failure due to the action of free radicals, and reduce ventilator-induced lung injury and the risk of disability resulting from fibrotic changes within the lungs. Melatonin may also increase the efficacy of COVID-19 vaccination. The high safety profile of melatonin and its potential anti-SARS-CoV-2 effects make this molecule a preferable drug for treating sleep disturbances in COVID-19 patients. However, randomized clinical trials are needed to verify the clinical usefulness of melatonin in the treatment of COVID-19.


Assuntos
COVID-19/tratamento farmacológico , Melatonina/farmacologia , SARS-CoV-2/efeitos dos fármacos , COVID-19/virologia , Vacinas contra COVID-19/farmacologia , Citocinas/metabolismo , Sequestradores de Radicais Livres/metabolismo , Humanos , Melatonina/uso terapêutico , Sistema Renina-Angiotensina , Transtornos do Sono-Vigília/tratamento farmacológico
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