Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 68.965
Filtrar
4.
Indian J Pathol Microbiol ; 67(2): 489, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38817157
5.
Molecules ; 29(7)2024 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-38611875

RESUMO

Mamey (Mammea americana L.) is a tropical fleshy fruit native from the West Indies and northern South America. It is very appreciated for its flavor and color but has been little described. The present study investigates the composition and histochemistry of the pulp cell walls of three mamey accessions readily available in Martinique. The impact of pulp processing into puree on cell wall composition is evaluated. The histology and rheology of mamey puree are assessed considering these characterizations. Mamey pulp cell wall composition is dominated by highly methyl-esterified pectins (DM: 66.2-76.7%) of high molecular weight, and show few hemicelluloses, mainly xyloglucans. Processing reduced methyl-esterified uronic acid contents and gave purees with significantly different viscosities. Mamey puree was composed of polydisperse particles (20-2343 µm), which size distributions were different depending on the accession: Ti Jacques was dominated by smaller particles (50% had approximated diameters lower than 160 µm), Sonson's by larger particles (50% had approximated diameters higher than 900 µm), and Galion's had an intermediate profile. This new knowledge on mamey pulp is valuable for future works on mamey processing into new food products, even more so for those including cell wall polysaccharide-degrading enzymes.


Assuntos
Mammea , Parede Celular , Alimentos , Histocitoquímica , Peso Molecular
6.
Zebrafish ; 21(2): 177-180, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38621207

RESUMO

Lectins are carbohydrate-binding proteins with specific affinity to glycoconjugates expressed in various tissues. Lectins are of substantial utility as research, histochemical, and diagnostic tools in mammalian systems. Reactivity of 12 commonly used plant-based lectins was studied in zebrafish liver. Four lectins, tomato lectin (TL), wheat germ agglutinin, concanavalin A, and Jacalin showed strong reactivity to hepatic parenchymal structures. Importantly, TL reacted to glycoconjugates within segments of the larval and adult intrahepatic biliary network, from canaliculi to bile ducts. We provide evidence that lectins can serve as important histochemical tools to investigate the structural and functional characteristics of the zebrafish liver.


Assuntos
Lectinas , Peixe-Zebra , Animais , Peixe-Zebra/metabolismo , Histocitoquímica , Fígado/metabolismo , Glicoconjugados/metabolismo , Mamíferos/metabolismo
7.
Indian J Med Microbiol ; 49: 100573, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38556249

RESUMO

Actinomycosis is a rare infection caused by Actinomyces spp. Of all actinomycosis infections, only 5% of Hepatic Actinomycosis (HA) infection has been reported. This disease is often misdiagnosed as a malignancy. This case report presents a 45-year-old woman with diabetes, initially suspected of intrahepatic cholangiocarcinoma, but after careful tissue staining, we found the results supported HA infection.


Assuntos
Actinomyces , Actinomicose , Humanos , Actinomicose/diagnóstico , Actinomicose/microbiologia , Actinomicose/patologia , Actinomicose/tratamento farmacológico , Feminino , Pessoa de Meia-Idade , Actinomyces/isolamento & purificação , Fígado/patologia , Fígado/microbiologia , Hepatopatias/microbiologia , Hepatopatias/diagnóstico , Hepatopatias/patologia , Histocitoquímica , Tomografia Computadorizada por Raios X
8.
Acta Trop ; 254: 107195, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38548212

RESUMO

A clinical case of an adult horse with invasive, ulcerative, proliferative, pyogranulomatous disease of the skin (tumor) in the shoulder region is presented. The mass had a granulomatous and crater-shaped appearance, with serosanguinous discharge and the presence of fistulas with caseous material. The tumor was removed by surgery and sent to the laboratory for diagnosis. Histopathology was performed using Grocott-Gomori methenamine silver stain. The presence of necrotic material, fibrosis, infiltrated cells, and brown-colored hyphae, characteristic of members of the genus Pythium, were observed. To identify the infecting species, conventional PCRs for the amplification of the ITS-1 was carried out. Histopathological and PCR tests confirmed infection by a Pythium insidiosum strain closely associated with previous records from the US and Central America. Our report represents the first molecularly confirmed case of equine pythiosis in Mexico.


Assuntos
Doenças dos Cavalos , Pitiose , Pythium , Animais , Pitiose/diagnóstico , Pitiose/microbiologia , Pitiose/patologia , Cavalos , Pythium/isolamento & purificação , Pythium/genética , Pythium/classificação , Doenças dos Cavalos/parasitologia , Doenças dos Cavalos/microbiologia , Doenças dos Cavalos/diagnóstico , México , Reação em Cadeia da Polimerase , DNA Espaçador Ribossômico/genética , Masculino , Histocitoquímica , Pele/patologia , Pele/microbiologia , Pele/parasitologia
9.
Microsc Microanal ; 30(2): 392-400, 2024 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-38502789

RESUMO

Biomineralization of brain tissues occurs both in normal and pathological conditions. Dura mater biomineralization is widespread and occurs in 1-72% of cases, depending on the patient's age and research method. The amount of biomineral deposits under the conditions of tumor growth in the meninges only increases, reaching 100% in the case of psammomatous meningiomas. Since calcifications are often found in the meninges, the problem of differential diagnosis with calcified meningiomas arises. A total of 30 samples of meningiomas with signs of biomineralization-dense structure, characteristic crunch, psammoma bodies (group I) and 30 samples of meningiomas without any signs of biomineralization were examined as controls (group II). To detect pathological biomineralization, the meningioma tissue was studied using the methods of macroscopic description, histology, histochemistry, and immunohistochemistry, scanning electron microscopy with microanalysis, and transmission electron microscopy. A significantly higher level of caspase3 and features of the expression of osteoblastic markers (a lower level of OPG expression and a higher level of the presence of RANKL in group I, the absence of fluctuations in the expression of SPARC) may indicate a dystrophic type of development of biomineral deposits in meningiomas.


Assuntos
Biomineralização , Imuno-Histoquímica , Meningioma , Meningioma/patologia , Meningioma/metabolismo , Humanos , Imuno-Histoquímica/métodos , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Idoso , Pessoa de Meia-Idade , Feminino , Masculino , Adulto , Histocitoquímica/métodos , Calcinose/patologia
10.
Indian J Pathol Microbiol ; 67(2): 445-448, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38391363

RESUMO

ABSTRACT: A 19-year-old woman presented with painless lower abdominal discomfort and a cystic-solid mass measuring 15.9 cm on the right ovary. She subsequently underwent laparoscopic right ovarian cystectomy. Microscopic examination of the mass showed the typical morphological features of Wilms' tumor and the predominance of teratoid elements constituting more than 50% of the tumor. To date, few cases of extrarenal teratoid Wilms' tumor (TWTs) in adults have been reported in the literature. The case presented in the present is the third reported case of adult extrarenal TWT occurring in ovary.


Assuntos
Neoplasias Ovarianas , Tumor de Wilms , Feminino , Humanos , Adulto Jovem , Histocitoquímica , Laparoscopia , Microscopia , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/cirurgia , Neoplasias Ovarianas/diagnóstico , Ovário/patologia , Ovário/cirurgia , Teratoma/patologia , Teratoma/cirurgia , Teratoma/diagnóstico , Teratoma/diagnóstico por imagem , Tumor de Wilms/patologia , Tumor de Wilms/cirurgia , Tumor de Wilms/diagnóstico
11.
Indian J Pathol Microbiol ; 67(2): 396-400, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38391333

RESUMO

ABSTRACT: Synovial sarcoma (SS) is rarely documented in the female genital tract, especially confirmed by molecular testing for SYT::SSX translocation and TLE1 immunostaining. A 62-year-old lady presented with a progressively increasing lump and pain over her right groin, for 6-month duration. Radiologically, a well-defined, solid-cystic mass was seen involving the right labia with necrotic areas, sparing the underlying muscles and the overlying skin. She underwent a biopsy followed by a surgical excision. Histopathologic examination revealed a spindle cell sarcoma, including tumor cells exhibiting a prominent hemangiopericytomatous pattern. There were focal areas of epithelial differentiation (pseudoglandular) along with areas of round cell morphology and increased mitoses (poor differentiation) in the resected specimen. Immunohistochemically, the tumor cells were diffusely positive for TLE1, patchily positive for pan keratin (AE1/AE3) and EMA, the latter more in the areas of epithelial differentiation, while negative for CD34, SMA, desmin, S100P, and SOX10. INI1/SMARCB1 showed a characteristic weak to absent (mosaic) staining pattern. Furthermore, the tumor displayed SS18::SSX 1 fusion by RT-PCR. This constitutes one of the few reported cases of vulvar SS, confirmed by molecular testing and the first documented vulvar SS showing a mosaic pattern of INI1/SMARCB1 immunostaining. A review of the literature and diagnostic implications are presented herewith.


Assuntos
Imuno-Histoquímica , Proteína SMARCB1 , Sarcoma Sinovial , Vulva , Neoplasias Vulvares , Humanos , Feminino , Sarcoma Sinovial/genética , Sarcoma Sinovial/diagnóstico , Sarcoma Sinovial/patologia , Pessoa de Meia-Idade , Proteína SMARCB1/genética , Neoplasias Vulvares/patologia , Neoplasias Vulvares/diagnóstico , Neoplasias Vulvares/genética , Vulva/patologia , Proteínas de Fusão Oncogênica/genética , Biomarcadores Tumorais/genética , Histocitoquímica , Microscopia , Proteínas Correpressoras/genética , Proteínas Proto-Oncogênicas , Proteínas Repressoras
12.
Indian J Pathol Microbiol ; 67(2): 463-465, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38391355

RESUMO

ABSTRACT: Extranodal Natural killer/T (NK/T) cell lymphoma nasal type is an aggressive non-Hodgkin lymphoma and has a constant association with Epstein-Barr virus (EBV) infection. Approximately more than 75% cases are located in upper aero-digestive tract, of which stomach is a very rare site. Very few cases of gastric extranodal NK/T cell lymphoma have been reported in the literature. A 22-year-old male patient presented with complaints of abdominal pain and hematemesis. Endoscopy showed a large ulcer in the stomach. Partial gastrectomy done and histopathology showed transmural infiltration by intermediate size atypical lymphoid cells which are immunopositive for CD3, CD56, TIA, EBV-encoded RNA (EBER) and negative for CD4, CD8, CD20. A diagnosis of extranodal NK/T cell lymphoma nasal type was made.


Assuntos
Linfoma Extranodal de Células T-NK , Neoplasias Gástricas , Humanos , Masculino , Linfoma Extranodal de Células T-NK/patologia , Linfoma Extranodal de Células T-NK/diagnóstico , Neoplasias Gástricas/patologia , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/cirurgia , Adulto Jovem , Imuno-Histoquímica , Gastrectomia , Estômago/patologia , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/isolamento & purificação , Histocitoquímica , Microscopia
13.
Indian J Pathol Microbiol ; 67(2): 459-462, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38391360

RESUMO

ABSTRACT: Extranodal nasal-type natural killer (NK)/T-cell lymphoma is a type of non-Hodgkin lymphoma. Neoplastic lymphocytes are positive for CD4, CD56, and CD20, a specific B-cell marker. CD20 positive NK/T-cell lymphoma is rare, with only nine reported cases. This paper reports a case of nasal-type NK/T-cell lymphoma with CD20 positivity in a 47-year-old woman. The patient presented with bilateral nasal congestion and bloody nasal cavity secretions for 2 months. Computed tomography revealed thickening of the nasal mucosa and posterior wall of the nasopharyngeal crest, and the left and right cervical lymph nodes were enlarged. On histopathology, the lesion was composed of medium-sized atypical lymphoid cells and vascular infringement. Immunohistochemical staining showed that the tumor cells were positive for CD20, CD3, CD56, and Epstein-Barr virus (EBV)-encoded RNA in situ hybridization. The patient was treated with radiotherapy for 2 months and is currently well.


Assuntos
Antígenos CD20 , Imuno-Histoquímica , Linfoma Extranodal de Células T-NK , Humanos , Feminino , Antígenos CD20/análise , Pessoa de Meia-Idade , Linfoma Extranodal de Células T-NK/patologia , Linfoma Extranodal de Células T-NK/diagnóstico , Tomografia Computadorizada por Raios X , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/isolamento & purificação , Hibridização In Situ , Microscopia , Histocitoquímica , Antígeno CD56/análise , Complexo CD3/análise , Resultado do Tratamento , Biomarcadores Tumorais/genética , Radioterapia , RNA Viral/genética , Neoplasias Nasais/patologia , Neoplasias Nasais/diagnóstico
14.
Eur J Histochem ; 68(1)2024 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-38389457

RESUMO

For over a century, Palazzo Botta (Palace Botta) has housed the University of Pavia's Biomedical Institutes. Illustrious scientists have conducted research and taught at this Palace, making significant contributions to the advancement of natural, biological, and medical science. Among them, Camillo Golgi received the Nobel Prize for discovering the so-called "black reaction." Following Golgi, the Palace continued to be a hub for the development of methodologies and reactions aimed at detecting and quantifying biological components. Maffo Vialli (in the Golgi stream) was the first to establish a Histochemistry Research Group, which began in the naturalistic field and later expanded to the biomedical area. Among the many histochemical studies initiated in the Palace, the Feulgen reaction undoubtedly played a significant role. This reaction, developed R. Feulgen and H. Rossenbeck in 1924, had significant international implications: numerous researchers then contributed to define its fine chemical details, which remained the subject of study for years, resulting in a massive international scientific literature. The Pavia School of Histochemistry also contributed to the evolution and application of this method, which has become a true benchmark in quantitative histochemistry. Giovanni Prenna and the CNR Centre for Histochemistry made significant contributions, as they were already focused on fluorescence cytochemistry. The Pavia researchers made significant contributions to the development of methodology and, in particular, instrumentation; the evolution of the latter resulted in the emergence of flow cytometry and an ever-increasing family of fluorescent probes, which somewhat overshadowed the Feulgen reaction for DNA quantification. The advent of monoclonal antibodies then contributed to the final explosion of flow cytometry in clinical application, almost making young neophytes forget that its roots date back to Feulgen.


Assuntos
DNA , Corantes de Rosanilina , Histocitoquímica/história , Corantes Fluorescentes
15.
Indian J Pathol Microbiol ; 67(2): 405-407, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38391300

RESUMO

ABSTRACT: Gastroduodenal perforation commonly due to spontaneous perforation of a pre-existing peptic ulcer is a surgical emergency. On laparotomy, approximately 60%-70% perforations are duodenal and 15%-20% gastric. The most prevalent etiology are Helicobacter pylori infection in 65%-70% and non-steroidal anti-inflammatory drugs (NSAIDS) abuse in 30%-50% cases depending on the prevalence of H. pylori infection. We report here the autopsy findings in a 29-year-old male who collapsed suddenly in the emergency room of our hospital after a bout of massive hematemesis.


Assuntos
Autopsia , Úlcera Duodenal , Úlcera Péptica Perfurada , Humanos , Masculino , Adulto , Úlcera Duodenal/complicações , Úlcera Duodenal/diagnóstico , Úlcera Péptica Perfurada/complicações , Evolução Fatal , Hematemese/etiologia , Duodeno/patologia , Histocitoquímica
19.
Indian J Pathol Microbiol ; 67(2): 438-440, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38391340

RESUMO

ABSTRACT: Cryptococcosis usually occurs in immunocompromised patients and presents as meningitis and lung disease. Adrenal gland involvement may be observed, yet primary adrenal insufficiency by cryptococcal infection is infrequent. We present a case of a middle-aged immunocompetent man with primary adrenal insufficiency and bilateral adrenal lesions, splenomegaly, and miliary mottling in the lungs on imaging. No evidence of meningitis was witnessed. The clinico-radiological findings led toward the differential diagnosis of disseminated tuberculosis or fungal infection. Detection of cryptococcus organism was done on fine-needle aspiration cytology and biopsy on periodic acid-Schiff stain and Gomori`s methenamine silver stain. Thus, it is recommended to keep the possibility of cryptococcosis in mind while dealing with instances that have a tuberculosis-like clinico-radiological presentation. The detection of the causal organism on Fine needle aspiration (FNA)/biopsy examination may be useful in confirming the diagnosis and determining the appropriate medical treatment.


Assuntos
Glândulas Suprarrenais , Insuficiência Adrenal , Criptococose , Humanos , Masculino , Criptococose/diagnóstico , Criptococose/tratamento farmacológico , Criptococose/patologia , Biópsia por Agulha Fina , Insuficiência Adrenal/diagnóstico , Pessoa de Meia-Idade , Glândulas Suprarrenais/patologia , Glândulas Suprarrenais/diagnóstico por imagem , Glândulas Suprarrenais/microbiologia , Cryptococcus/isolamento & purificação , Diagnóstico Diferencial , Tomografia Computadorizada por Raios X , Pulmão/patologia , Pulmão/diagnóstico por imagem , Microscopia , Doenças das Glândulas Suprarrenais/diagnóstico , Doenças das Glândulas Suprarrenais/microbiologia , Imunocompetência , Histocitoquímica
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...