Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 126
Filtrar
1.
Trop Biomed ; 39(3): 412-420, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36214438

RESUMO

This study aimed to consider the in vitro and in vivo effects of the Stachys lavandulifolia methanolic extract (SLME) (2.5, 5, 10, 25, 50, 100 µg/mL) against Leishmania major infection. The in vitro antileishmanial effects of SLME was studies on promastigote and amastigote forms of L. major. The effect of SLME on the nitric oxide (NO) and apoptosis, secretion of Th1/2 cytokines, and infectivity rate in macrophages cells were also studies. The cytotoxicity of SLME on human (THP-1) and murine (J774-A1 cell) macrophage cells was investigated through the measuring the 50% cytotoxic concentrations (CC50). Moreover, the in vivo effects of SLME for healing the cutaneous leishmaniasis (CL) lesions in infected BALB/c mice studied by assessing the lesions size and the parasite load during four weeks of treatment. The calculated 50% inhibitory concentration (IC50) valuesfor SLME and meglumine antimoniate (MA) against the promastigote stage were 23.4 and 71.1 µg/mL, respectively. For amastigote stage, the IC50 values for SLME and MA were 39.3 µg/mL and 44.3 µg/mL, respectively. Followed by 28 days' topically therapy with SLME at doses of 50 and 100 mg/kg/day, the CL lesions size as well as parasite load were significantly (p<0.001) reduced; such that the recovery percentage of the infected mice was 80% and 97% after treatment with SLME at the dose of 50 and 100 mg/kg, respectively. SLME also markedly induced the NO production and apoptosis; whereas decreased infection rate in macrophage cells. After incubation of infected macrophages with SLME, the level interferon gamma was meaningfully (p<0.001) elevated as a dose-dependent response; in contrast, release of interleukin 10 (IL-10) and IL-4 markedly (p<0.001) decreased. The CC50 value for SLME against THP-1 and J774-A1 cell was 996.4 µg/mL and 741.3 µg/mL, respectively. The calculated selectivity index of >10 for SLME and MA confirmed their specificity to amastigotes and the low toxicity for macrophages. Our results showed the potent effects of SLME in eliminating and controlling Leishmania parasites in both in vitro and in vivo assays. Based on the current experimental study, SLME can be suggested as an alternative medicine for the isolation and production of a new agent for treating CL caused by L. major. Although, we found some cellular mechanisms of SLME against Leishmania parasites, but, additional surveys are necessary to specify the accurate mechanisms of action, toxicity, and its efficacy mainly in human subjects.


Assuntos
Antiprotozoários , Leishmania major , Leishmaniose Cutânea , Stachys , Animais , Antiprotozoários/farmacologia , Antiprotozoários/uso terapêutico , Citocinas , Humanos , Interferon gama , Interleucina-10 , Interleucina-4 , Leishmaniose Cutânea/tratamento farmacológico , Antimoniato de Meglumina/uso terapêutico , Camundongos , Camundongos Endogâmicos BALB C , Óxido Nítrico , Extratos Vegetais/farmacologia
2.
Mol Biol Rep ; 49(9): 8251-8258, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36002657

RESUMO

Cytotoxic activities of methanolic crude extract of Stachys parviflora (Lamiaceae family) and its sub-fractions were primarily evaluated against human breast adenocarcinoma (MCF-7 and MDA-MB-231) and prostate (PC3) cell lines. The methanolic extract exhibited the highest activity, and was chosen for the isolation procedure. Four diterpenoid quinones, namely miltirone [1], tanshinone IIA [2], 1-hydroxy-tanshinone IIA [3], and cryptotanshinone [4] were isolated. Notably, this is the first report on the isolation and/or characterization of the mentioned diterpenoids from the Stachys genus. In this study, 1-hydroxy-tanshinone IIA [3] displayed the highest cytotoxicity among the isolated compounds. The mechanism of the cytotoxicity of methanolic extract and isolated compounds was further investigated by the utilization of propidium iodide staining (PI) assay. The results showed that the methanolic extract and 1-hydroxy-tanshinone IIA [3] enhanced DNA fragmentation in PC3 and MCF-7 cells. Moreover, the western blotting analysis demonstrated increasing and decreasing protein levels of Bax and Bcl2, respectively, and cleaved poly ADP-ribose polymerase (PARP). Further bioassay-guided phytochemical assessments of S. parviflora can be suggested as a promising approach for discovering potent bioactive secondary metabolites.


Assuntos
Antineoplásicos Fitogênicos , Neoplasias da Mama , Diterpenos , Stachys , Abietanos , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Apoptose , Western Blotting , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Diterpenos/farmacologia , Humanos , Masculino , Próstata
3.
Phytochemistry ; 203: 113373, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35977603

RESUMO

The Stachys L. genus has been used in traditional medicine to treat skin inflammations, stomach disorders, and stress. The aim of this study was to investigate the chemical profile and biological activity of the methanolic extract of Stachys spreitzenhoferi Heldr. (Lamiaceae) aerial parts, collected on the island of Kythira, South Greece. The analysis by liquid chromatography coupled with electrospray ionization and high-resolution mass spectrometry [LC-(-)ESI/HRMSn] of the methanol extract revealed the occurrence of thirty-six compounds - flavonoids, phenylethanoid glycosides, iridoids, quinic acid derivatives, aliphatic alcohol glycosides, and oligosaccharides - highlighting the substantial presence, as main peaks, of the iridoid melittoside (2) along with flavonoid compounds such as 4'-O-methylisoscutellarein mono-acetyl-diglycoside/chrysoeriol mono-acetyl-diglycoside (24), trimethoxy- (35) and tetramethoxyflavones (36). This extract was tested for its antimicrobial properties against Gram-positive and negative pathogenic strains. The extract was not active against Gram-negative bacteria tested, but it possessed a good dose-dependent antimicrobial activity towards S. aureus (MIC: 1.0 mg/mL) and L. monocytogenes (MIC: 1.0 mg/mL) Gram-(+) strains. Furthermore, this extract has been tested for its possible antioxidant activity in vitro. In particular, it has been shown that these molecules cause a decrease in DPPH, ABTS, and H2O2 radicals. The extract of S. spreitzenhoferi exhibited anti-DPPH activity (IC50: 0.17 mg/mL), anti-H2O2 activity (IC50: 0.125 mg/mL), and promising antiradical effect with an IC50 value of 0.18 mg/mL for anti-ABTS activity. S. spreitzenhoferi extract caused a decrease in ROS (at the concentration of 200 µg/mL) and an increase in the activity of the antioxidant enzymes SOD, CAT, and GPX in OZ-stimulated PMNs. Furthermore, it exhibited antiproliferative activity against acute myeloid leukemia (U937 cell), causing 50% of cell death at the 0.75 mg/mL.


Assuntos
Anti-Infecciosos , Lamiaceae , Stachys , Antibacterianos , Antioxidantes/química , Antioxidantes/farmacologia , Flavonoides/química , Flavonoides/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Grécia , Humanos , Iridoides , Metanol , Componentes Aéreos da Planta , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ácido Quínico , Espécies Reativas de Oxigênio , Stachys/química , Staphylococcus aureus , Superóxido Dismutase , Células U937
4.
Phytochem Anal ; 33(6): 915-942, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35670362

RESUMO

INTRODUCTION: The genus Stachys L., belonging to the family Lamiaceae, is one of the largest genera with remarkable medicinal properties. Plants of this genus produce a broad range of secondary metabolites. OBJECTIVES: Due to the incomplete comprehensive assessment of chemical profiles in Stachys species, we conducted an untargeted metabolomics study and identified potential biomarkers in the six sections of Stachys with chemotaxonomic importance. MATERIAL AND METHODS: Dried leaves of 17 taxa were utilized for analysis of all the constituents using HPLC-MQ-API-MS. The obtained data were processed and analyzed using multivariate statistical methods, including heatmaps, PLS-DA score plots, functional analysis of metabolic pathways, metabolite set enrichment analysis, and biomarker and network analysis. RESULTS: Among the 129 metabolites, 111 flavonoids and 18 non-flavonoids were recognized. The most represented flavonoids, including 41 flavones and 20 flavonols, displayed remarkable abundance. In non-flavonoid compounds, a total of six coumarins and six phenolic acids were present at high levels. In terms of approved markers in six sections, 76 chemical compounds, mainly flavonoids, coumarins, quinic acids, and cinnamic acids, were identified as potential biomarkers or chemotaxonomic indicators. Accordingly, the taxonomic complexities of some Stachys species in sections Fragilicaulis, Aucheriana, and Setifolia were properly resolved. CONCLUSION: An HPLC-MS/MS-based metabolomics approach integrated with multivariate statistical methods was employed to identify (1) valuable markers and analyze metabolic diversity and (2) predict the pharmaceutical properties of Stachys species. The obtained chemical profiles provide a new perspective for investigation of the Stachys genus.


Assuntos
Lamiaceae , Stachys , Biomarcadores , Cromatografia Líquida de Alta Pressão/métodos , Cumarínicos/análise , Flavonoides/análise , Metabolômica , Stachys/química , Espectrometria de Massas em Tandem/métodos
5.
Food Funct ; 13(3): 1551-1562, 2022 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-35072202

RESUMO

Microbial contamination and nitrite accumulation are the two major concerns in the quality control of fermented vegetables. In the present study, a lactic acid bacteria strain Lactobacillus plantarum ZJ316 (ZJ316) was inoculated during Stachys sieboldii Miq. (SSM) fermentation, and the effects of ZJ316 on the quality and bacterial community of SSM during fermentation were investigated. It was observed that ZJ316 could avoid the occurrence of the nitrite peak and maintain the nitrite content of fermented SSM at a low level. Gas chromatography-mass spectrometry (GC-MS) results suggested that ZJ316 gave good flavor to the fermented SSM. 16S rDNA sequencing showed that Firmicutes was the dominant flora after ZJ316 inoculation, and the abundance of Proteobacteria decreased at the same time. At the level of the genus, SSM fermented by ZJ316 had a more obvious inhibitory effect on Pseudomonas on the 7th day compared with the naturally fermented SSM. Additionally, the effect of ZJ316-fermented SSM on gut microbiota modulation was also evaluated using an in vitro fecal fermentation system. The results revealed that ZJ316 had a relatively subtle influence on intestinal communities with a potentially positive impact on probiotics such as Lactobacillus and Bifidobacterium and a negative impact on Enterobacteriaceae. Furthermore, SSM fermented by ZJ316 promoted the production of short-chain fatty acids (SCFAs) in the human intestine. These results demonstrate that L. plantarum ZJ316 can be used as a good starter in the fermentation process of pickles.


Assuntos
Alimentos Fermentados , Lactobacillus plantarum , Probióticos , Stachys , Manipulação de Alimentos , Microbiologia de Alimentos , Microbioma Gastrointestinal/efeitos dos fármacos , Humanos
6.
Clin Rheumatol ; 41(4): 1033-1044, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34766228

RESUMO

BACKGROUND: Stachys schtschegleevii (SSC) is a herbal medicine used to treat infections. To date, this is the first study aimed to investigate the effects of SSC tea on disease activity score (DAS), serum inflammatory biomarkers and matrix metalloproteinases (MMP-1 and MMP-3) among women with rheumatoid arthritis (RA). METHODS: This pilot, triple-blind, randomized controlled clinical trial was conducted among forty-four women (age: 30-65 years) diagnosed with moderately active RA. Subjects were randomly assigned (1:1 ratio) into either SSC group (2.4 g/day SSC + 2.4 g/day black tea, n=22) or placebo (2.4 g/day black tea, n=22) for 8 weeks. Serum high-sensitivity C-reactive protein (hs-CRP), interleukin-1 beta (IL-1ß), and MMPs were measured using ELISA. According to the American College of Rheumatology guideline considering hs-CRP, DAS28 was assessed. RESULTS: Both study groups had respondent rates above 94.9%. The SSC intervention caused significant reductions in the number and the percent changes of the tender joints (SSC: -74.39% vs. placebo: -57.15%, mean differences= -0.77; P<0.05) and DAS28 [SSC: -32.44% vs. placebo: -22.32%, mean differences= -0.41, P<0.05). Unlike the intervention within SSC group that showed significant reductions in the mean serum levels of hs-CRP, IL-1ß, and MMP-3, SSC caused significant MMP-3 reductions (SSC: -20.59% vs. placebo: 1.29%, P<0.05). CONCLUSION: The SSC intervention showed an appropriate clinical efficacy for female RA patients, accompanying remarkable reductions in the number of tender and swollen joints, DAS28, and serum levels of MMP-3. This can provide additional insights to the interventional studies controlling RA-related pathological and inflammatory outcomes. Trial registration Prospectively registered at the Iranian Registry of Clinical Trials (IRCT), linked to the WHO Registry Network ( https://en.irct.ir/trial/11602 , IRCT registration number: IRCT2015032011335N5, Registration date:2015-05-12). Key Points • Stachys schtschegleevii improved clinical outcomes and attenuated disease severity in RA patients. • Stachys schtschegleevii ameliorated serum level of MMP-3 in RA patients.


Assuntos
Artrite Reumatoide , Stachys , Adulto , Idoso , Artrite Reumatoide/tratamento farmacológico , Método Duplo-Cego , Feminino , Humanos , Irã (Geográfico) , Metaloproteinase 3 da Matriz , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Chá
7.
Oxid Med Cell Longev ; 2021: 8141563, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34707781

RESUMO

Herbal medicines harbor essential therapeutic agents for the treatment of cholestasis. In this study, we have assessed the anticholestatic potential of Stachys pilifera Benth's (SPB's) hydroalcoholic extract encapsulated into liposomes using bile duct ligation- (BDL-) induced hepatic cholestasis in rats. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), malondialdehyde (MDA), total thiol (T-SH) content, protein carbonyl (PCO), total bilirubin (TBIL), albumin (ALB), and nitric oxide (NO) metabolite levels were measured in either liver tissue or plasma to assess liver damage. Moreover, expression of proinflammatory cytokines (IL-1ß and TNF-α) and liver fibrosis markers (TGF-ß and SM-α) which are driving forces of many liver disorders was also determined. The activity of AST, ALT, and ALP was significantly enhanced in the BDL group in comparison to the control group; however, treatment with liposomal (SPB) hydroalcoholic extract significantly reduced AST and ALT's activity. Increases in MDA, TBIL, and NO levels and T-SH content due to BDL were restored to control levels by liposomal (SPB) hydroalcoholic extract treatment. Similarly, hepatic and plasma oxidative marker MDA levels, significantly enhanced by BDL, were significantly decreased by liposomal (SPB) hydroalcoholic extract treatment. Moreover, histopathological findings further demonstrated a significant decrease in hepatic damage in the liposomal (SPB) hydroalcoholic extract-treated BDL group. In addition, liposomal (SPB) hydroalcoholic extract treatment decreased the liver expression of inflammatory cytokines (IL-1ß, TNF-α) and liver fibrosis markers (TGF-ß and SM-α). Since liposomal (SPB) hydroalcoholic extract treatment alleviated the BDL-induced injury of the liver and improved the hepatic structure and function more efficiently in comparison to free SPB hydroalcoholic extract, probable liposomal (SPB) hydroalcoholic extract exhibits required potential therapeutic value in protecting the liver against BDL-caused oxidative injury.


Assuntos
Antioxidantes/farmacologia , Colestase Intra-Hepática/tratamento farmacológico , Fígado/efeitos dos fármacos , Extratos Vegetais/farmacologia , Stachys , Actinas/genética , Actinas/metabolismo , Animais , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Antifibróticos/isolamento & purificação , Antifibróticos/farmacologia , Antioxidantes/isolamento & purificação , Colestase Intra-Hepática/metabolismo , Colestase Intra-Hepática/patologia , Ducto Colédoco/cirurgia , Citocinas/genética , Citocinas/metabolismo , Modelos Animais de Doenças , Mediadores da Inflamação/metabolismo , Ligadura , Lipossomos , Fígado/metabolismo , Fígado/patologia , Cirrose Hepática Biliar/tratamento farmacológico , Cirrose Hepática Biliar/metabolismo , Cirrose Hepática Biliar/patologia , Masculino , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Carbonilação Proteica/efeitos dos fármacos , Ratos Wistar , Stachys/química , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/metabolismo
8.
Biotechnol Prog ; 37(6): e3200, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34346569

RESUMO

Today, bacterial cellulose has received a great deal of attention for its medical applications due to its unique structural properties such as high porosity, good fluid uptake, good strength, and biocompatibility. This study aimed to fabricate and study bacterial cellulose/graphitic carbon nitride/nettles/trachyspermum nanocomposite by immersion and PVA/BC/g-C3 N4 /nettles/trachyspermum nanofiber by electrospinning method as a wound dressing. The g-C3 N4 and g-C3 N4 solution were synthesized and then were characterized using Fourier transform infrared, X-ray diffraction, Zeta Potential, and scanning electronic microscope analyzes. Also, the antibacterial properties of the synthesized materials were proved by gram-positive and gram-negative bacteria using the minimum inhibitory concentration method. Besides, the toxicity, migration, and cell proliferation results of the synthesized materials on NIH 3T3 fibroblasts were evaluated using MTT and scratch assays and showed that the BC/PVA/g-C3 N4 /nettles/trachyspermum composite not only had no toxic effect on cells but also contributed to cell survival, cell migration, and proliferation has done. To evaluate the mechanical properties, a tensile strength test was performed on PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers, and the results showed good strength of the nanocomposite. In addition, in vivo assay, the produced nanofibers were used to evaluate wound healing, and the results showed that these nanofibers were able to accelerate the wound healing process so that after 14 days, the wound healing percentage showed 95%. Therefore, this study shows that PVA/BC/g-C3 N4 /nettles/trachyspermum nanofibers effectively inhibit bacterial growth and accelerate wound healing.


Assuntos
Antibacterianos , Bandagens , Celulose , Grafite , Compostos de Nitrogênio , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Apiaceae/química , Bactérias/química , Bactérias/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Celulose/química , Celulose/farmacologia , Grafite/química , Grafite/farmacologia , Camundongos , Testes de Sensibilidade Microbiana , Células NIH 3T3 , Nanofibras/química , Compostos de Nitrogênio/química , Compostos de Nitrogênio/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Álcool de Polivinil/química , Stachys/química
9.
Food Chem Toxicol ; 155: 112374, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34186120

RESUMO

This work analysed the chemical composition, antioxidant, and enzyme inhibitory activities of solvent extract (SJ-ME) and fractions (SJ-HF, SJ-EAF, and SJ-MF) of the Stachys riederi var. japonica (Miq.) (SJ). Furthermore, the effect of SJ-EAF in STZ induced type 2 diabetic mice was examined. Among the samples, SJ-EAF exhibited a lower IC50 concentration of 64.2 ± 0.48 µg/mL for DPPH and 82.6 ± 0.09 µg/mL for ABTS+. The SJ-EAF concentration of 2.89 ± 0.03 µg and 2.27 ± 0.98 µg was equivalent to 1 µg of acarbose mediated enzyme inhibitory effect against α-amylase and α -glucosidase, respectively. The SJ-EAF did not show cytotoxicity (<80%) to NIH3T3 nor HepG2 cells but enhanced the glucose uptake in the IR-HepG2. LC-MS/MS of SJ-EAF showed the presence of a total of 16 compounds. Among the identified compounds, rosmarinic acid, caffeic acid, oleanolic acid, and ursolic acid showed high catalytic activity of α-amylase and α-glucosidase. The treatments of SJ-EAF restored the level of blood glucose, body weight, insulin, HDL and mRNA level of IRS1, GLUT2, GLUT4 and Akt whereas it reduced the excess elevation of total cholesterol, total triglycerides, LDL, AST, ALT, ALP, BUN, and creatinine in STZ induced diabetic mice. Overall, the present study concluded that the SJ-EAF exhibited promising antidiabetic activity.


Assuntos
Antioxidantes/uso terapêutico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/uso terapêutico , Extratos Vegetais/uso terapêutico , Stachys/química , Animais , Antioxidantes/química , Antioxidantes/metabolismo , Antioxidantes/toxicidade , Linhagem Celular Tumoral , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Diabetes Mellitus Tipo 2/induzido quimicamente , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/patologia , Expressão Gênica/efeitos dos fármacos , Humanos , Hipoglicemiantes/química , Hipoglicemiantes/metabolismo , Hipoglicemiantes/toxicidade , Masculino , Camundongos Endogâmicos ICR , Simulação de Acoplamento Molecular , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Extratos Vegetais/toxicidade , Ligação Proteica , Estreptozocina , alfa-Amilases/metabolismo , alfa-Glucosidases/metabolismo
10.
FEMS Microbiol Lett ; 368(9)2021 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-33970244

RESUMO

Stachys pilifera Benth is an endemic species of Stachys family found in Iran with a wide application as an herbal tea. The objective of this research was to evaluate the antimicrobial, antioxidant and cytotoxic activity of the essential oil from the aerial parts of S. pilifera. Essential oil (EO) composition analysis showed that cis-Chrysanthenyl acetate (24.9%), viridiflorol (18.3%), trans-Caryophyllene (9.8%), caryophyllene oxide (4.6%), α-terpineol (3.3%) and linalool (3.1%) were the most abundant components. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the EO showed a higher antimicrobial activity against Gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) than Gram-negative organisms (Escherichia coli, Shigella sonnei, Pseudomonas aeruginosa and Salmonella enterica subsp. Enterica). The antioxidant activity of EO was studied using DPPH, FRAP and ß-carotene/linoleic acid assays. IC50 for the DPPH, FRAP and ß-carotene/linoleic acid tests were 23.2, 28.7 and 16.1 µg/mL, respectively, that it was higher than the results for BHT (P ≤ 0.05). The cytotoxic activity of the EO was evaluated using HT29 and HUVEC cells and it was observed that by increasing in EO concentration from 0.026 to 19.4 ug/mL, the viability of the cells for HT29 and HUVEC reduced to 6.8 and 7.1%, respectively. The results from this study suggest the possibility to use the essential oils from S. pilifera Benth as a natural preservative in processed or packaged food due to its high antibacterial, antioxidant and cytotoxic activities.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Óleos Voláteis/análise , Óleos Voláteis/farmacologia , Folhas de Planta/química , Stachys/química , Antibacterianos/química , Antioxidantes/química , Bactérias/classificação , Sobrevivência Celular/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Células HT29 , Humanos , Testes de Sensibilidade Microbiana , Chás de Ervas
11.
Environ Sci Pollut Res Int ; 28(32): 44098-44110, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33843001

RESUMO

Industrial activities can affect accumulation of pollutants (e.g., heavy metals (HMs)) by plants and influence their entrance to the food chain. This research was carried out on accumulation of HMs including chromium (Cr), lead (Pb), and zinc (Zn) by two plants Stachys inflata and Scariola orientalis grown in natural grasslands in vicinity of a national park where they are influenced by dispersion of particulate matter (PM) through Sarooj Cement Factory in central Iran. The PM spatial dispersion of the factory was determined using the AERMOD model. Soil and vegetation samples were collected based on the modeled PM levels to analyze their HM contents. Bioconcentration factor (BCF) and transfer factor (TF) from root to shoot were determined in two widespread plants of the region: Stachys inflata and Scariola orientalis. The mean concentration of HMs in the soil samples was as follows: Zn (145.39 mg/kg) > Pb (78.52 mg/kg) > Cr (32.69 mg/kg) which was significantly correlated with simulated PM concentrations. This indicated the common source and distribution pattern of HMs which affected their accumulation in plants. TF and BCF values of the HMs were higher in Scariola orientalis than those of Stachys inflata, especially for Cr showing potentially higher risk to enter the food chain. The results showed that HM concentration in the soil as well as their accumulation by plants were correlated with the simulated PM deposition and not with linear distance from the factory.


Assuntos
Metais Pesados , Poluentes do Solo , Stachys , Bioacumulação , China , Monitoramento Ambiental , Irã (Geográfico) , Metais Pesados/análise , Material Particulado , Solo , Poluentes do Solo/análise
12.
An Acad Bras Cienc ; 93(3): e20200641, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33909754

RESUMO

Stachys species belonging to Lamiaceae family have been used for medicinal purposes since ancient times. The aim of the present study was to investigate the chemical compositions and antibacterial, anti-tyrosinase activities of the essential oil of Stachys macrostachya. The essential oil was prepared by hydrodistillation method using a Clevenger-type apparatus and chemical composition was determined by gas chromatography (GC). The antibacterial activity of essential oil was performed by the disc diffusion and microdilution broth method against five Gram-positive and two Gram-negative bacteria. The tyrosinase inhibitory activity was evaluated by minor modifications of Masuda's method. According to the results of GC analyses, twenty-three compounds were identified representing 91.9% of the total volatile composition. The main compounds were germacrene D (12.2%), globulol (10.9%), α-pinene (9.7%), and valencene (7.6%). The present study showed that the tested essential oil of S. macrostachya exhibited antibacterial activity against Acinetobacter baumannii (MIC 62.50 µg/mL) and tyrosinase inhibition activity (IC50 22.86 ± 0.82 µg/mL). These results suggest that the essential oil could be exploited as a potential source of natural antimicrobial agents of this bacterium as well as tyrosinase inhibitors.


Assuntos
Lamiaceae , Óleos Voláteis , Stachys , Antibacterianos/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Testes de Sensibilidade Microbiana , Óleos Voláteis/farmacologia
13.
Nat Prod Res ; 35(15): 2588-2592, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31651198

RESUMO

Stachys pilifera Benth is an endemic species of Iran where it is found in the mountainous habitats of Zagros region. The plant is a rich source of secondary metabolites endowed with different medicinal properties. Here, for the first time, the essential oil variability among eleven wild populations of S. pilifera, was investigated. Results indicated that the variation of the chemical profile of essential oils was remarkable. The most abundant components were cis-chrysanthenyl acetate (19.1-48.2%), viridiflorol (1.4-19.1%), trans-caryophyllene (2.3-11.9%), caryophyllene oxide (1.9-11.0%), limonene (2.0- 5.9%) and spathulenol (0.0- 9.5). Based on the cluster analyses (CA), four main chemotypes were recognized: chemotype I (cis-chrysanthenyl acetate), chemotype II (cis-chrysanthenyl acetate/viridiflorol), chemotype III (cis-chrysanthenyl acetate/viridiflorol/spathulenol), chemotype IV (cis-chrysanthenyl acetate/trans-caryophyllene/α-pinene). The chemical variation detected can be useful to consider these populations for pharmaceutical industries and industrial applications as well as for domestication and conservation purposes.


Assuntos
Limoneno/química , Óleos Voláteis , Stachys , Irã (Geográfico)
14.
Comb Chem High Throughput Screen ; 24(2): 177-186, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32538719

RESUMO

AIMS AND OBJECTIVES: The intake of Stachys sieboldii MIQ. has been associated with relieving inflammation and maintaining optimal gut health function. We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice. In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues. MATERIALS AND METHODS: 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base. The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit. Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix. RESULTS: Mice fed on S. sieboldii MIQ. showed significantly reduced counts of aerobic and coliform in the feces compared with control. 16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ. increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.) in feces compared with that in the control (P<0.05 for all). Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05). The fecal fatty acid composition in the S. sieboldii MIQ. group showed a higher percentage of 6:0 and 18:2n-6 compared with that in the control group (P<0.05). The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ. group (P<0.05). No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues. CONCLUSION: The present results showed that supplementation of mice with S. sieboldii MIQ. increased beneficial gut microflora and decreased harmful microflora. Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.


Assuntos
Citocinas/biossíntese , Microbioma Gastrointestinal/efeitos dos fármacos , Raízes de Plantas/química , Polissacarídeos/farmacologia , Stachys/química , Animais , Peso Corporal/efeitos dos fármacos , Suplementos Nutricionais , Fezes/química , Camundongos , Polissacarídeos/química
15.
J Biomol Struct Dyn ; 39(17): 6452-6466, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-32731792

RESUMO

Stachys species are considered as important medicinal plants with numerous health benefit effects. In continuation of our research on the Greek Stachys species, the chemical profile of the aerial parts of cultivated S. iva Griseb. has been explored. The NMR profiles of the plant extract/infusion were used to guide the isolation process, leading to the targeted isolation of seventeen known compounds. The rare acylated flavonoid, stachysetin, was isolated for the third time from plant species in the international literature. Identification of the characteristic signals of stachysetin in the 1D 1H-NMR spectrum of the crude extract was presented. In order to evaluate the potential of the identified chemical space in Stachys to bear possible bioactivity against diabetes, we performed an in silico screening against 17 proteins implicated in diabetes, as also ligand based similarity metrics against established anti-diabetic drugs. The results capitalized the anti-diabetic potency of stachysetin. Its binding profile to the major drug carrier plasma protein serum albumin was also explored along with its photophysical properties suggesting that stachysetin could be recognized and delivered in plasma through serum albumin and also could be tracked through near-infrared imaging. Communicated by Ramaswamy H. Sarma.


Assuntos
Hipoglicemiantes/farmacologia , Extratos Vegetais/farmacologia , Stachys , Flavonoides/farmacologia , Espectroscopia de Ressonância Magnética , Simulação de Acoplamento Molecular , Stachys/química
16.
Nat Prod Res ; 35(22): 4861-4864, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32146843

RESUMO

Lung cancer leading cause of humans' cancer-related death remains incurable with current drug therapies having many side-effects. Finding new natural cancer remedies would help design better therapies. This study aims to investigate the anticancer and antioxidant effects of the Levant endemic plants Prango sasperula, Stachys ehrenbergii, Hirtellina lobelia, and Salvia multicaulis on human pulmonary adenocarcinoma. GC/MS and NMR analyzed the bioactive compounds of methanolic extracts of these plants. Their antioxidant effect was measured by ABTS assay. The effect of these extracts on the viability of A549 (Human lung adenocarcinoma cell line) and NHBE (Normal Human Bronchial Epithelial Cells) was examined using MTT and Anchorage-Independent Colony Formation assays. Our findings show an interesting, specific cytotoxic effect of these plant extracts on A549 cells. Additionally, S. ehrenbergii and H. lobelii present a specific high alkylation potential. Data reveal an interesting antitumor potential attributed to their antiproliferative and alkylation actions towards human pulmonary adenocarcinoma.


Assuntos
Adenocarcinoma de Pulmão , Adenocarcinoma , Stachys , Adenocarcinoma/tratamento farmacológico , Adenocarcinoma de Pulmão/tratamento farmacológico , Antioxidantes/farmacologia , Proliferação de Células , Humanos , Extratos Vegetais/farmacologia
17.
Environ Technol ; 42(24): 3747-3755, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32149570

RESUMO

The unique properties of nanoparticles create broad opportunities as regards their application in almost all disciplines of science and technology. There are many reports about the negative influence of nanoproducts on the environment and humans. Therefore, it is of vital importance to explore the impact of metal nanoparticles on plants. This is why this work is concerned with the phytotoxic activity of ZnO nanoparticles synthesized biologically from Betonica officinalis extract against the seed of Lepidium sativum, Linum flavum, Zea mays and Salvia hispanica-Chia. The obtained ZnO nanoparticles were characterized by UV-Vis, Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and Atomic Force Microscopy (AFM). Those methods made it possible to assess the structure and size of the obtained ZnO nanoparticles, which was 5 nm. The obtained ZnO nanoparticles exhibited significant toxic properties throughout the range of the tested concentrations. ZnO nanoparticles were the most toxic to Lepidium sativum, for which the IC50 value was 0.0000112 [mg/ml]. The solution of Zn(NO3)2 was toxic as well, as it inhibited the growth of the tested sample throughout the range of the tested concentrations.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Stachys , Óxido de Zinco , Antibacterianos , Humanos , Nanopartículas Metálicas/toxicidade , Testes de Sensibilidade Microbiana , Extratos Vegetais/toxicidade , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X , Óxido de Zinco/toxicidade
18.
Prep Biochem Biotechnol ; 51(4): 395-404, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32940554

RESUMO

Stachys sieboldii MiQ (SSM) is an important food and medicinal herb in Korea, used to improve memory of patients with senile dementia and cardiovascular diseases. However, little information on bioactive components from SSM or standardized extraction methods for these components is available. This study isolated and purified major components from SSM for the first time, and assessed their ability to inhibit soluble epoxide hydrolase (sEH). The results showed that acteoside is the most potent inhibitor of sEH, with an IC50 of 33.5 ± 0.5 µM. Additional active components, including harpagide, tryptophan, and 8-acetate-harpagide, along with acteoside, were tentatively identified using high-performance liquid chromatography photodiode array tandem mass spectrometry (HPLC-PDA-MS/MS) and quantified using an ultraviolet detector at 210 nm. Further, an ultrasonic-assisted extraction technique for extraction of four bioactive compounds in SSM was developed and optimized using response surface methodology (RSM). The optimal extraction conditions were: extraction time, 30.46 minutes; extraction temperature, 67.95 °C, and methanol concentration 53.85%. The prediction model of RSM was validated with laboratory experiments. The similarity between predicted and actual values was 97.84%. The extraction method is thus a rapid, environment-friendly, energy-saving method can be applied to extract bioactive components from SSM in large quantities.


Assuntos
Epóxido Hidrolases/antagonistas & inibidores , Epóxido Hidrolases/química , Extração Líquido-Líquido/métodos , Modelos Estatísticos , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Stachys/química , Cromatografia Líquida de Alta Pressão/métodos , Glucosídeos/isolamento & purificação , Glucosídeos/farmacologia , Concentração Inibidora 50 , Glicosídeos Iridoides/isolamento & purificação , Glicosídeos Iridoides/farmacologia , Metanol/química , Fenóis/isolamento & purificação , Fenóis/farmacologia , Piranos/isolamento & purificação , Piranos/farmacologia , Solubilidade , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos , Temperatura , Triptofano/isolamento & purificação , Triptofano/farmacologia , Ondas Ultrassônicas
19.
Proc Natl Acad Sci U S A ; 117(43): 26600-26607, 2020 10 27.
Artigo em Inglês | MEDLINE | ID: mdl-33028678

RESUMO

When pollen grains become exposed to the environment, they rapidly desiccate. To protect themselves until rehydration, the grains undergo characteristic infolding with the help of special structures in the grain wall-apertures-where the otherwise thick exine shell is absent or reduced in thickness. Recent theoretical studies have highlighted the importance of apertures for the elastic response and the folding of the grain. Experimental observations show that different pollen grains sharing the same number and type of apertures can nonetheless fold in quite diverse fashions. Using the thin-shell theory of elasticity, we show how both the absolute elastic properties of the pollen wall and the relative elastic differences between the exine wall and the apertures play an important role in determining pollen folding upon desiccation. Focusing primarily on colpate pollen, we delineate the regions of pollen elastic parameters where desiccation leads to a regular, complete closing of all apertures and thus to an infolding which protects the grain against water loss. Phase diagrams of pollen folding pathways indicate that an increase in the number of apertures leads to a reduction of the region of elastic parameters where the apertures close in a regular fashion. The infolding also depends on the details of the aperture shape and size, and our study explains how the features of the mechanical design of apertures influence the pollen folding patterns. Understanding the mechanical principles behind pollen folding pathways should also prove useful for the design of the elastic response of artificial inhomogeneous shells.


Assuntos
Pólen/química , Pólen/fisiologia , Fenômenos Biomecânicos/fisiologia , Dessecação , Elasticidade/fisiologia , Pólen/anatomia & histologia , Pólen/metabolismo , Stachys/citologia , Stachys/fisiologia
20.
Nutrients ; 12(7)2020 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-32664607

RESUMO

This study aimed at investigating the anti-obesity and anti-dyslipidemic effects of Stachys sieboldii Miq. root (SS) powder in rats following a high-fat and high-cholesterol (HFC) diet for 6 weeks. Thirty-two Sprague-Dawley rats were fed one of the following diets: a regular diet (RD), HFC, HFC supplemented with 3% SS (HFC + 3SS) or HFC supplemented with 5% SS (HFC + 5SS). Following an HFC diet increased body weight (BW) gain (p < 0.001) and the food efficiency ratio (FER; p < 0.001); however, SS consumption gradually prevented the HFC-induced BW gain (p < 0.001) and increase in FER (p < 0.01). The HFC diet resulted in increased liver size (p < 0.001) and total adipose tissue weight (p < 0.001), whereas the SS supplementation decreased hepatomegaly (p < 0.05) and body fat mass (p < 0.001). SS consumption prevented the increased activities of serum alanine aminotransferase (ALT; p < 0.001), aspartate aminotransferase (AST; p < 0.001), alkaline phosphatase (ALP; p < 0.01 in HFC + 5SS) and lactate dehydrogenase (LDH; p < 0.001 in HFC + 5SS) induced by the HFC diet (p < 0.001). The SS supplementation improved lipid profiles in the circulation by lowering triglyceride (TG; p < 0.01), total cholesterol (TC; p < 0.001) and non-HDL cholesterol (non-HDL-C; p < 0.001) levels, as well as the atherogenic index (p < 0.01) and cardiac risk factor (p < 0.01). The lipid distribution in the liver (p < 0.05) and white adipose tissues (WAT; p < 0.001) of the HFC + SS diet-consuming rats was remarkably lower than that of the HFC diet-consuming rats. The average size of the epididymal adipose tissue (p < 0.001) was significantly lower in the HFC + SS diet-fed rats than in the HFC diet-fed rats. The fecal lipid (>3% SS; p < 0.001) and cholesterol (5% SS; p < 0.001) efflux levels were significantly elevated by the SS supplementation compared to those measured in the RD or HFC diet-fed groups. In addition, the hepatic lipid and cholesterol metabolism-related gene expressions were affected by SS consumption, as the hepatic anabolic gene expression (Acc; p < 0.001, Fas; p < 0.001 and G6pdh; p < 0.01) was significantly attenuated. The HFC + 5SS diet-fed rats exhibited elevated hepatic Cyp7a1 (p < 0.001), Hmgcr (p < 0.001) and Ldlr (p < 0.001) mRNA expression levels compared to the HFC diet-fed rats. These results suggest that SS may possess anti-adipogenic and lipid-lowering effects by enhancing lipid and cholesterol efflux in mammals.


Assuntos
Adipogenia/efeitos dos fármacos , Fármacos Antiobesidade , Anticolesterolemiantes , Colesterol na Dieta/efeitos adversos , Dieta Hiperlipídica/efeitos adversos , Suplementos Nutricionais , Dislipidemias/tratamento farmacológico , Dislipidemias/etiologia , Fitoterapia , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Stachys/química , Aumento de Peso/efeitos dos fármacos , Animais , Dislipidemias/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/metabolismo , Masculino , Ratos Sprague-Dawley
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...