Vesicular stomatitis due to Indiana III (Alagoas/VSIV-3) is endemic in Brazilian state of Ceará / Estomatite vesicular causada pelo subtipo Indiana III (Alagoas/VVI-3) é endêmica no estado do Ceará, Brasil

Although Northeast Brazil is considered free of foot and mouth disease (FMD) with vaccination, several economic and health damages are still recorded due to the occurrence of vesicular syndromes that can be evaluated, such as Vesicular Stomatitis (VS). Therefore, this study aimed to confirm the occurrence of this disease and to determine the predominant viral serotype in suspected cases notified to the Official Veterinary Service of Ceará in 2013 performing official diagnostic protocols recommended by the World Organization for Animal Health. After clinical and epidemiological investigation in 46 farms, 32 probable cases of VS were considered with 78 sampled animals, 65 bovines and 13 equines. Serum (54) and epithelium (24) samples were collected. Six (14.6%) of 41 bovines and 8 (61.5%) of 13 equines described seroconversion to Indiana Vesiculovirus (IVV) by viral neutralization. The IVV was detected in 15 (62.5%) of 24 bovines epithelia using the indirect sandwich ELISA. Finally, positive epithelium underwent complement fixation test viral subtyping that identified the occurrence of Indiana III serotype (Alagoas/IVV-3) in 11 (73.3%) of 15 previous positives cattle. These were the first confirmed cases of VS in Ceará with an official diagnosis of IVV-3, confirming the endemic character attributed to the state through previous unofficial serological surveys. The presence of VS is a continuing diagnostic challenge, given the risk of possible incursions of FMD. Vesicular stomatitis is recurrent and is a worrying in this area free of foot and mouth disease with vaccination that bring damage to producers and a maximum alert to the Sanitary Defense Organs in the face of a probable case of vesicular syndrome.(AU)

Embora o Nordeste do Brasil seja considerado livre de Febre Aftosa (FA) com a vacinacao, ainda sao registrados varios prejuízos econômicos e sanitários devido a ocorrencia de sindromes vesiculares que precisam ser adequadamente avaliadas, como Estomatite Vesicular (EV). Portanto, este estudo teve como objetivo confirmar a ocorrencia desta doenca e determinar o sorotipo viral predominante em casos suspeitos notificados ao Servico Veterinario Oficial do Ceara no ano de 2013 realizando protocolos oficiais de diagnóstico recomendados pela Organizacao Mundial de Saude Animal. Após investigação clínica e epidemiológica em 46 propriedades rurais, foram considerados 32 casos prováveis de propriedade foco de EV com 78 animais amostrados, sendo 65 bovinos e 13 equídeos. Amostras de soro (54) e epitelio (24) foram coletadas. Analises sorologicas de 6 (14,6%) de 41 bovinos e 8 (61,5%) de 13 equídeos apresentaram soroconversao ao Vesiculovirus Indiana (VVI) por neutralizacao viral. O VVI foi detectado em 15 (62,5%) de 24 epitélios bovinos usando ELISA indireto sanduiche. Por fim, amostras de epitélio positivas foram submetidas a subtipagem viral por fixacao do complemento que identificou a ocorrência do sorotipo Indiana III (Alagoas/VVI-3) em 11 (73,3%) de bovinos. Estes foram os primeiros casos confirmados de EV no estado do Ceara com diagnostico oficial de VVI-3 confirmando o carater endemico atribuido ao Estado por meio de levantamentos sorologicos nao oficiais anteriores. A presença de EV é um desafio diagnóstico contínuo, dado o risco de possíveis incursões de FA. A Estomatite Vesicular é recorrente e preocupante nesta área livre de FA com vacinação pois, além de prejuízos aos produtores, traz um alerta máximo aos Órgãos de Defesa Sanitária diante de um caso provável de síndrome vesicular.(AU)

Seroprevalence of Corynebacterium pseudotuberculosis and Toxoplasma gondii in sheep in semi-arid region of Ceará state / Soroprevalência de Corynebacterium pseudotuberculosis de Toxoplasma gondii em ovinos de região semi-árida do estado do Ceará

The present study was conducted to investigate in 20 extensive sheep farms for the seroprevalence of Corynebacterium pseudotuberculosis (n=402) and Toxoplasma gondii (n=228). Enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies to C. pseudotuberculosis/T. gondii. It was observed that C. pseudotuberculosis showed the highest prevalence in the region (34.07%) with statistically significant presence (p 0.05) in ewes. Antibodies to T. gondii was reported in 14.91% of the animals studied. About C. pseudotuberculosis/T. gondii coinfection the categories of rams showed significant (p 0.05) differences, suggesting that this gender could perpetuate the diseases in the flocks. It was concluded that the knowledge about the diseases in the region under study would facilitate the execution of prophylactic measures, especially against the diseases that pose risks to the public health and cause damages to the producer.(AU)

O presente estudo foi conduzido para investigar a soroprevalência em 20 fazendas de criação extensiva de ovinos quanto à presença de anticorpos para Corynebacterium pseudotuberculosis (n=402) e Toxoplasma gondii (n=228). Ensaio de imunoabsorção enzimático (ELISA) foi utilizado para a detecção de anticorpos contra C. pseudotuberculosis/T. gondii. Observou-se que C. pseudotuberculosis apresentou a maior prevalência na região (34,07%), com presença estatisticamente significante (p 0,05) nas categorias de ovelha. Anticorpos contra T. gondii foram encontrados em 14,91% dos animais estudados. Sobre a coinfecção de C. pseudotuberculosis/T. gondii, as categorias carneiro apresentaram diferenças significativas (p 0,05), sugerindo que esse gênero poderia perpetuar as doenças nos rebanhos. Concluiu-se que o conhecimento sobre as doenças na região em estudo facilitaria a execução de medidas profiláticas, principalmente contra as doenças que apresentam riscos à saúde pública e causam danos ao produtor.(AU)

Exostose cartilaginosa múltipla em um canino pediátrico / Multiple cartilaginous exostoses in a pediatric dog

Background: Multiple cartilaginous exostosis (MCE) is a rare neoplastic disease in dogs that is characterized by the presence of osteochondromas in multiple bones. MCE is a disorder of development during growth and maturation, the realetiology of which has yet to be elucidated, but is believed to have a genetic basis. Usually affected animals have a historyof a palpable mass on the bone surface, causing anatomical deformities and compression of adjacent structures. SinceMCE is a rare neoplastic disease in dogs it may be difficult to recognize in the clinic. The aim of this study was to reporta case of MCE in a pediatric dog.Case: A female 4-month-old Rottweiler dog with a history of bone neoformation on the left hind limb, anorexia, weightloss, and difficulty walking received clinical care. The disease had its initial onset 2 months prior. At physical examination, the animal showed paresis of the hind limbs and a bony tumor on the lateral part of the left tibia. A complete bloodcell count and serum biochemical profile were obtained. The only abnormality diagnosed was hyperproteinemia due tohyperglobulinemia. Other diagnostic tests were not authorized and the animal was euthanized. At the necropsy, an intrathoracic neoformation was observed from the sixth to the eighth rib on the right antimer. Some tumors were also observedon the fourth lumbar vertebra and between the first and the second sacral vertebra, and a left tibial tumor, which had beenobserved at physical examination. The sagittal section of the spine revealed the presence of extradural compression of thespinal cord due to vertebral proliferations with compression of the nerve roots. All of the proliferations had macroscopicsimilarities, being firm, sessile, irregular, and with complete adherence to deep planes. Tumor samples were submitted tohistopathological analysis and the tissue morphology was compatible with osteochondroma...

Exostose cartilaginosa múltipla em um canino pediátrico / Multiple cartilaginous exostoses in a pediatric dog

Background: Multiple cartilaginous exostosis (MCE) is a rare neoplastic disease in dogs that is characterized by the presence of osteochondromas in multiple bones. MCE is a disorder of development during growth and maturation, the realetiology of which has yet to be elucidated, but is believed to have a genetic basis. Usually affected animals have a historyof a palpable mass on the bone surface, causing anatomical deformities and compression of adjacent structures. SinceMCE is a rare neoplastic disease in dogs it may be difficult to recognize in the clinic. The aim of this study was to reporta case of MCE in a pediatric dog.Case: A female 4-month-old Rottweiler dog with a history of bone neoformation on the left hind limb, anorexia, weightloss, and difficulty walking received clinical care. The disease had its initial onset 2 months prior. At physical examination, the animal showed paresis of the hind limbs and a bony tumor on the lateral part of the left tibia. A complete bloodcell count and serum biochemical profile were obtained. The only abnormality diagnosed was hyperproteinemia due tohyperglobulinemia. Other diagnostic tests were not authorized and the animal was euthanized. At the necropsy, an intrathoracic neoformation was observed from the sixth to the eighth rib on the right antimer. Some tumors were also observedon the fourth lumbar vertebra and between the first and the second sacral vertebra, and a left tibial tumor, which had beenobserved at physical examination. The sagittal section of the spine revealed the presence of extradural compression of thespinal cord due to vertebral proliferations with compression of the nerve roots. All of the proliferations had macroscopicsimilarities, being firm, sessile, irregular, and with complete adherence to deep planes. Tumor samples were submitted tohistopathological analysis and the tissue morphology was compatible with osteochondroma...(AU)

Serological and molecular investigation of Leishmania spp. infection in cats from an area endemic for canine and human leishmaniasis in Northeast Brazil / Investigação sorológica e molecular da infecção por Leishmania spp. em gatos provenientes de uma área endêmica para leishmaniose canina e humana no nordeste brasileiro

The aim of this study was to investigate the occurrence of Leishmania spp. antibodies, and its association with feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), in domestic cats from an area endemic for canine and human leishmaniasis in Rio Grande do Norte State, Brazil. Ninety-one cats were subjected to a complete clinical exam, and blood samples were collected. An epidemiological questionnaire was used to investigate the risk factors. IgG anti-Leishmania spp. antibodies were detected by immunofluorescence antibody test (IFAT), with a cut-off value of 1:40. Polymerase chain reaction (PCR) was performed to detect genetic material of Leishmania spp. in the blood samples. The presence of antibodies against FIV and antigens of FeLV was evaluated using an immunochromatographic test. Seropositivity for Leishmania spp., FIV, and FeLV was observed in 14/91 (15.38%), 26/91 (28.57%), and 3/91 (3.29%) cats, respectively. All samples gave negative results on PCR analysis. Based on these data, no significant statistical association was observed between seropositivity for Leishmania spp., and sex, age, presence of clinical signs, evaluated risk factors, and positivity for retroviruses. These findings demonstrated for the first time that cats from Mossoró, Rio Grande do Norte, are being exposed to this zoonosis and might be part of the epidemiological chain of transmission of visceral leishmaniasis.(AU)

O objetivo do presente estudo foi investigar a ocorrência de anticorpos contra Leishmania spp., e sua associação com o vírus da imunodeficiência felina (FIV) e o vírus da leucemia felina (FeLV), em felinos domésticos provenientes de uma área endêmica no estado do Rio Grande do Norte, para a leishmaniose visceral canina e humana. Noventa e um gatos foram submetidos a exame clínico completo e amostras de sangue foram coletadas. Um questionário epidemiológico foi feito para investigar fatores de risco. Anticorpos IgG anti-Leishmania spp. foram identificados por meio da imunofluorescência indireta (RIFI), adotando-se como ponto de corte a diluição de 1:40. A reação em cadeia da polimerase (PCR) foi executada visando detectar o material genético de Leishmania spp. a partir de amostras de sangue total. Para avaliar a presença de anticorpos contra o FIV e antígenos do FeLV foi utilizado um teste imunocromatográfico. Observou-se soropositividade em 14/91 (15,38%), 26/91 (28,57%) e 3/91 (3,29%) animais para Leishmania spp., FIV e FeLV, respectivamente. Nenhuma amostra foi positiva na PCR. Baseado nestes dados, não foi observada nenhuma associação estatística significativa entre a soropositividade para Leishmania spp. e gênero, idade, presença de sinais clínicos, fatores de risco avaliados e positividade para as retroviroses. Esses achados demonstram pela primeira vez que felinos da cidade Mossoró, Rio Grande do Norte, estão sendo expostos a esta zoonose, sugerindo que os mesmos podem estar participando da cadeia epidemiológica de transmissão da leishmaniose visceral.(AU)

Eficácia do oclacitinib no manejo da síndrome da atopia felinaˆipt / Efficacy of oclacitinib on feline atopic syndrome managementˆien

Background:The feline atopic syndrome (FAS) associated to environmental allergens is the third most common allergic dermatosis in domestic cats. In general, clinical signs are not pathognomonic and the exclusion of other pruritus causes is necessary to reach the diagnosis of FAS. The treatment is based on the use of drugs to control the pruritus, such as glucocorticoids, cyclosporine and, recently, oclacitinib, a Janus kinase inhibitor. This study aimed to report the efficacy of oclacitinib on the treatment of FAS associated to environmental allergens. Case:A 10-year-old female feline, crossbred, presented a history of pruritic dermatitis during ten months and diarrhea. The animal had been submitted to treatment for ectoparasites with pour-on selamectin and was fed with a commercial hypoallergenic diet in the last eight weeks or so. However, no improvement on the skin condition was observed. Physical examination revealed disseminated furfuraceous desquamation, excoriation and erythema on the right supraorbital region. Bilateral conjunctivitis was also observed. Complete blood cell count, biochemistry profile, urinalysis, immunochroma-thographic test for feline immunodeffiency virus (FIV) and feline leukemia virus (FeLV), fungic culture and abdominal ultrasonography were requested. The abnormalities observed were reduced urinary density and discrete loss of renal corticomedullary differentiation. Thus, based on physical examination and complementary exams, the animal was diag-nosis with FAS, since the main other causes of pruritus (hypersensitivity to ectoparasites and alimentary allergens) were excluded. The animal was also diagnosed with stage 1 chronic kidney disease. Therapy based on oclacitinib was instituted with an induction dose of 1 mg/kg twice daily for 14 days, followed by a maintenance dose of 1 mg/kg once daily. After 30 days of treatment, a satisfactory therapeutic response was observed, with complete remission of pruritus. The...(AU)

Eficácia do oclacitinib no manejo da síndrome da atopia felinaˆipt / Efficacy of oclacitinib on feline atopic syndrome managementˆien

Background:The feline atopic syndrome (FAS) associated to environmental allergens is the third most common allergic dermatosis in domestic cats. In general, clinical signs are not pathognomonic and the exclusion of other pruritus causes is necessary to reach the diagnosis of FAS. The treatment is based on the use of drugs to control the pruritus, such as glucocorticoids, cyclosporine and, recently, oclacitinib, a Janus kinase inhibitor. This study aimed to report the efficacy of oclacitinib on the treatment of FAS associated to environmental allergens. Case:A 10-year-old female feline, crossbred, presented a history of pruritic dermatitis during ten months and diarrhea. The animal had been submitted to treatment for ectoparasites with pour-on selamectin and was fed with a commercial hypoallergenic diet in the last eight weeks or so. However, no improvement on the skin condition was observed. Physical examination revealed disseminated furfuraceous desquamation, excoriation and erythema on the right supraorbital region. Bilateral conjunctivitis was also observed. Complete blood cell count, biochemistry profile, urinalysis, immunochroma-thographic test for feline immunodeffiency virus (FIV) and feline leukemia virus (FeLV), fungic culture and abdominal ultrasonography were requested. The abnormalities observed were reduced urinary density and discrete loss of renal corticomedullary differentiation. Thus, based on physical examination and complementary exams, the animal was diag-nosis with FAS, since the main other causes of pruritus (hypersensitivity to ectoparasites and alimentary allergens) were excluded. The animal was also diagnosed with stage 1 chronic kidney disease. Therapy based on oclacitinib was instituted with an induction dose of 1 mg/kg twice daily for 14 days, followed by a maintenance dose of 1 mg/kg once daily. After 30 days of treatment, a satisfactory therapeutic response was observed, with complete remission of pruritus. The...

Acute myeloid leukemia in a dog chronically infected with leishmania spp. and other infectious agents / Leucemia mieloide aguda em cão infectado cronicamente por Leishmania spp. e outros agentes infecciosos

Background: Rare studies have described the association of hematopoietic tumors and canine visceral leishmaniosis, however the association between the parasitary disease and neoplasia is still not well established in dogs. Thus, the aim of the present study was to report a case of acute myeloid leukemia (AML) in a dog infected by Leishmania spp. and other infectious agents.Case: A 8-year-old, male Poodle, was brought to the Veterinary Hospital from Universidade Federal Rural do Semi-Árido. The dog had a history of recurrent tick-borne diseases, such as anaplasmosis, over the previous ten months. On physical examination, pale mucosa, enlargement of popliteal lymph nodes, onychogryphosis, purulent nasal discharge, and bilateral blepharitis with purulent discharge were observed. The dog was skinny and infested with ticks. The blood cell count revealed normocytic, normochromic anemia and leukocytosis (38.000/mm3 ) with neutrophilia (30.020/mm3 ). Serum biochemical tests demonstrated hyperproteinemia due to hyperglobulinemia, hypoalbuminemia, and an albumin:globulin ratio of 0.30. The immunochromathographic test for leishmaniasis was negative. The alterations observed in the bone marrow cytological analysis were suggestive of AML, and Anaplasma spp., Hepatozoon spp., and amastigote forms of Leishmania spp. were observed inside bone marrow cells. After diagnosis, a decision to euthanize the animal was made.Discussion: Few studies have demonstrated the presence of hematopoietic neoplasia in dogs chronically and simultaneously infected with multiple pathogens. A case of multiple myeloma in a dog associated with infection by Ehrlichia canis, A. phagocytophilum, L. infantum, and Dirofilaria immitis is described. Another study reported B-cell lymphoma in a dog with E. canis and Histoplasma capsulatum infection.[...]

Acute myeloid leukemia in a dog chronically infected with leishmania spp. and other infectious agents / Leucemia mieloide aguda em cão infectado cronicamente por Leishmania spp. e outros agentes infecciosos

Background: Rare studies have described the association of hematopoietic tumors and canine visceral leishmaniosis, however the association between the parasitary disease and neoplasia is still not well established in dogs. Thus, the aim of the present study was to report a case of acute myeloid leukemia (AML) in a dog infected by Leishmania spp. and other infectious agents.Case: A 8-year-old, male Poodle, was brought to the Veterinary Hospital from Universidade Federal Rural do Semi-Árido. The dog had a history of recurrent tick-borne diseases, such as anaplasmosis, over the previous ten months. On physical examination, pale mucosa, enlargement of popliteal lymph nodes, onychogryphosis, purulent nasal discharge, and bilateral blepharitis with purulent discharge were observed. The dog was skinny and infested with ticks. The blood cell count revealed normocytic, normochromic anemia and leukocytosis (38.000/mm3 ) with neutrophilia (30.020/mm3 ). Serum biochemical tests demonstrated hyperproteinemia due to hyperglobulinemia, hypoalbuminemia, and an albumin:globulin ratio of 0.30. The immunochromathographic test for leishmaniasis was negative. The alterations observed in the bone marrow cytological analysis were suggestive of AML, and Anaplasma spp., Hepatozoon spp., and amastigote forms of Leishmania spp. were observed inside bone marrow cells. After diagnosis, a decision to euthanize the animal was made.Discussion: Few studies have demonstrated the presence of hematopoietic neoplasia in dogs chronically and simultaneously infected with multiple pathogens. A case of multiple myeloma in a dog associated with infection by Ehrlichia canis, A. phagocytophilum, L. infantum, and Dirofilaria immitis is described. Another study reported B-cell lymphoma in a dog with E. canis and Histoplasma capsulatum infection.[...](AU)

Clinical, laboratory and therapeutic aspects of canine idiopathic hypereosinophilic syndrome: case report

The idiopathic hypereosinophilic syndrome (IHES) is a rare disease, characterized by variable eosinophilia and its massive infiltration into various organs. This study aimed to report clinical-laboratory findings and therapy in a canine with IHES. A one-year, 10-month-old male Rottweiler dog had a history of emesis, weight loss, hyporexia, and persistent eosinophilia on prior hematological exams. Vaccinations and worming were up to date. A complete blood count, serum biochemistry, bone marrow cytology, serology for leishmaniasis, chromatographic immunoassay for the detection of Dirofilaria immitis antigen, and abdominal ultrasound were requested. The tests for infectious diseases were negative. Blood biochemistry revealed no significant changes. An intense eosinophilia was observed in the hematology. A large number of cell precursors of the eosinophilic lineage were detected in the bone marrow cytology. Abdominal ultrasound showed thickening of intestinal loops. Considering the clinical and laboratory findings, the diagnosis of IHES was defined. Prednisolone treatment was instituted. The recurrence of peripheral eosinophilia occurred on the 35th day after therapy initiation. At that time, we opted to suspend the use of prednisolone and indicate the administration of deflazacort. With follow-up, therapeutic success with deflazacort was demonstrated, promoting the complete regression of clinical and ultrasound signs. The last glucocorticoid was maintained, but with a gradual dose reduction. The recognition of clinical and laboratory manifestations related to canine IHES is essential to establish an adequate diagnosis and therapy. Deflazacort emerges as a promising drug for controlling this disease.

Sertolioma intra-abdominal em cão com repercussões sistêmicas e locais Intra-abdominal / Sertoli cell tumor in dog with local and systemic repercussions

Background: In dogs, abdominal testes have higher risk of malignant transformation, and the Sertoli cell tumor is the most common type of testicular cancer in the species. This neoplasia can lead to an increase on estrogen production, causing a paraneoplastic feminization syndrome with dermatologic, behavioral and blood repercussions. When located inside the abdomen, the Sertoli cell tumor can compress adjacent organs, worsening the prognosis. Thus, this study aimed to report a case of an intra-abdominal Sertoli cell tumor in a dog associated to multiple organic complications.Case: A 4-year-old male Shih-tzu was admitted at the Veterinary Hospital of Universidade Federal Rural do Semi-Árido (UFERSA), with a history of abdominal cryptorchidism and the development of dermatological signs, such as alopecia and hyperpigmentation, with an evolution of two months. At physical examination it was observed alopecia on lumbosacral region and on hind limbs, hyperpigmentation, gynecomastia and pendulous prepuce, suggestive of feminization syndrome. A firm mass was palpated on caudal abdomen. The following exams were requested: complete blood cell count, serum biochemistry (alanine aminotransferase, alkaline phosphatase, creatinine and urea), abdominal ultrasonography and fine needle aspiration cytology of the abdominal mass. Hematological and biochemistry profile were within the normal range for the species. On the ultrasonography, regular and rounded structures were observed, suggesting ectopic testes (left testis: 2.37 x 2.95 cm; right testis: 1.92 x 1.38 cm). The left testis presented characteristics of malignant transformation. The cytology of the abdominal mass suggested Sertoli cell tumor. The dog was submitted to an exploratory laparotomy. The right testis was atrophied and its orchiectomy was performed.[...]

Sertolioma intra-abdominal em cão com repercussões sistêmicas e locais Intra-abdominal / Sertoli cell tumor in dog with local and systemic repercussions

Background: In dogs, abdominal testes have higher risk of malignant transformation, and the Sertoli cell tumor is the most common type of testicular cancer in the species. This neoplasia can lead to an increase on estrogen production, causing a paraneoplastic feminization syndrome with dermatologic, behavioral and blood repercussions. When located inside the abdomen, the Sertoli cell tumor can compress adjacent organs, worsening the prognosis. Thus, this study aimed to report a case of an intra-abdominal Sertoli cell tumor in a dog associated to multiple organic complications.Case: A 4-year-old male Shih-tzu was admitted at the Veterinary Hospital of Universidade Federal Rural do Semi-Árido (UFERSA), with a history of abdominal cryptorchidism and the development of dermatological signs, such as alopecia and hyperpigmentation, with an evolution of two months. At physical examination it was observed alopecia on lumbosacral region and on hind limbs, hyperpigmentation, gynecomastia and pendulous prepuce, suggestive of feminization syndrome. A firm mass was palpated on caudal abdomen. The following exams were requested: complete blood cell count, serum biochemistry (alanine aminotransferase, alkaline phosphatase, creatinine and urea), abdominal ultrasonography and fine needle aspiration cytology of the abdominal mass. Hematological and biochemistry profile were within the normal range for the species. On the ultrasonography, regular and rounded structures were observed, suggesting ectopic testes (left testis: 2.37 x 2.95 cm; right testis: 1.92 x 1.38 cm). The left testis presented characteristics of malignant transformation. The cytology of the abdominal mass suggested Sertoli cell tumor. The dog was submitted to an exploratory laparotomy. The right testis was atrophied and its orchiectomy was performed.[...](AU)

Clinical, laboratory and therapeutic aspects of canine idiopathic hypereosinophilic syndrome: case report

The idiopathic hypereosinophilic syndrome (IHES) is a rare disease, characterized by variable eosinophilia and its massive infiltration into various organs. This study aimed to report clinical-laboratory findings and therapy in a canine with IHES. A one-year, 10-month-old male Rottweiler dog had a history of emesis, weight loss, hyporexia, and persistent eosinophilia on prior hematological exams. Vaccinations and worming were up to date. A complete blood count, serum biochemistry, bone marrow cytology, serology for leishmaniasis, chromatographic immunoassay for the detection of Dirofilaria immitis antigen, and abdominal ultrasound were requested. The tests for infectious diseases were negative. Blood biochemistry revealed no significant changes. An intense eosinophilia was observed in the hematology. A large number of cell precursors of the eosinophilic lineage were detected in the bone marrow cytology. Abdominal ultrasound showed thickening of intestinal loops. Considering the clinical and laboratory findings, the diagnosis of IHES was defined. Prednisolone treatment was instituted. The recurrence of peripheral eosinophilia occurred on the 35th day after therapy initiation. At that time, we opted to suspend the use of prednisolone and indicate the administration of deflazacort. With follow-up, therapeutic success with deflazacort was demonstrated, promoting the complete regression of clinical and ultrasound signs. The last glucocorticoid was maintained, but with a gradual dose reduction. The recognition of clinical and laboratory manifestations related to canine IHES is essential to establish an adequate diagnosis and therapy. Deflazacort emerges as a promising drug for controlling this disease.(AU)

Canine juvenile cellulitis: a retrospective study (2009-2016) / Celulite juvenil canina: Estudo retrospectivo (2009-2016)

Juvenile cellulitis (JC) is an uncommon vesiculopustular disease that affects dogs, especially those under four months of age. The aim of the present study was to characterize the epidemiological, clinical, and therapeutic profile of JC based on clinical records of canine patients treated at the Veterinary Hospital of the Universidade Federal Rural do Semi-Árido, between the years 2009 and 2016. A total of five cases were diagnosed. Four dogs presented at an average age of two months and 22 days and were crossbred; the other dog was a rottweiler aged 38 weeks. The average time of evolution of the disease was 16 days, and the main clinical signs were edema, papules, pustules, and bilateral ocular discharge. The diagnosis was confirmed by cytology in four animals and by histopathology in only one patient. For all dogs, the treatment was based on cephalexin, administered for 30 days, and prednisolone for three weeks. Otological therapy was included when necessary. Four cases had a favorable outcome. JC is a rare dermatopathy that deserves special attention from veterinary practitioners.(AU)

A celulite juvenil (CJ) é uma doença vesiculopustular incomum que acomete cães principalmente na faixa etária inferior aos quatro meses. Este trabalho analisou o perfil epidemiológico, clínico e terapêutico da CJ em cães a partir da casuística do Hospital Veterinário da Universidade Federal Rural do Semi-Árido, referente aos anos de 2009 a 2016. Cinco casos foram diagnosticados, incluindo quatro cães (80%), sem raça definida e com idade média de dois meses e 22 dias, e um da raça Rottweiler (20%) com 38 meses de idade. O período evolutivo médio das lesões foi de 16 dias e os principais sinais clínicos observados foram edema, pápulas, pústulas e secreção ótica bilateral. O diagnóstico foi confirmado por citologia (80%) e histopatologia (20%). Em todos os animais, o tratamento consistiu de cefalexina, administrada por 30 dias, e prednisolona, por três semanas. A terapia otológica tópica foi incluída quando necessária. Em quatro casos o desfecho clínico foi favorável. A CJ é uma dermatopatia rara que merece atenção especial dos clínicos veterinários.(AU)

Canine juvenile cellulitis: a retrospective study (2009-2016) / Celulite juvenil canina: Estudo retrospectivo (2009-2016)

Juvenile cellulitis (JC) is an uncommon vesiculopustular disease that affects dogs, especially those under four months of age. The aim of the present study was to characterize the epidemiological, clinical, and therapeutic profile of JC based on clinical records of canine patients treated at the Veterinary Hospital of the Universidade Federal Rural do Semi-Árido, between the years 2009 and 2016. A total of five cases were diagnosed. Four dogs presented at an average age of two months and 22 days and were crossbred; the other dog was a rottweiler aged 38 weeks. The average time of evolution of the disease was 16 days, and the main clinical signs were edema, papules, pustules, and bilateral ocular discharge. The diagnosis was confirmed by cytology in four animals and by histopathology in only one patient. For all dogs, the treatment was based on cephalexin, administered for 30 days, and prednisolone for three weeks. Otological therapy was included when necessary. Four cases had a favorable outcome. JC is a rare dermatopathy that deserves special attention from veterinary practitioners.(AU)

A celulite juvenil (CJ) é uma doença vesiculopustular incomum que acomete cães principalmente na faixa etária inferior aos quatro meses. Este trabalho analisou o perfil epidemiológico, clínico e terapêutico da CJ em cães a partir da casuística do Hospital Veterinário da Universidade Federal Rural do Semi-Árido, referente aos anos de 2009 a 2016. Cinco casos foram diagnosticados, incluindo quatro cães (80%), sem raça definida e com idade média de dois meses e 22 dias, e um da raça Rottweiler (20%) com 38 meses de idade. O período evolutivo médio das lesões foi de 16 dias e os principais sinais clínicos observados foram edema, pápulas, pústulas e secreção ótica bilateral. O diagnóstico foi confirmado por citologia (80%) e histopatologia (20%). Em todos os animais, o tratamento consistiu de cefalexina, administrada por 30 dias, e prednisolona, por três semanas. A terapia otológica tópica foi incluída quando necessária. Em quatro casos o desfecho clínico foi favorável. A CJ é uma dermatopatia rara que merece atenção especial dos clínicos veterinários.(AU)

Survival of collared peccaries (Pecari tajacu) sperm after freezing-thawing using different extenders / Sobrevivência de espermatozoides de catetos (Pecari tajacu) após congelação-descongelação no uso de diferentes diluentes

Background: The development of semen cryopreservation protocols for collared peccaries (Pecari tajacu) would contribute to its preservation and multiplication. Nowadays, studies on this subject report the use of Tris and powdered coconutwater (ACP®-116c) as extenders. However, such studies are limited to the post-thawing immediate evaluation of samples.The knowledge on the sperm survival after thawing would be important in order to schedule the moment and the vial forartificial insemination. Therefore, the aim of the present research was to verify the effect of Tris and ACP extenders on thesperm survival of frozen-thawed semen, centrifuged or not, derived from collared peccaries.Materials, Methods & Results: Semen from 12 adult males collared peccaries were obtained by electroejaculation. Ejaculateswere immediately evaluated for sperm motility, vigor, viability, functional membrane integrity and morphology. Sampleswere diluted in Tris or ACP, both plus 10% egg yolk and 3% glycerol. Then, they were packaged into 0.25 mL straws, andstored in liquid nitrogen. After one week, samples were thawed at 38ºC/1 min and divided into two aliquots: one immediately diluted in the same extender, and the other submitted to centrifugation and then re-diluted. A thermorresistance testwas conducted including assessments at 0, 15, 30, 45, and 60 min as described for fresh semen. After analyzing the dataimmediately after thawing, we verify that coconut water provided a better preservation of sperm motility (41.3 ± 6.9%)and vigor (2.9 ± 0.2) than Tris, 33.1 ± 5.9% motile sperm with vigor 2.5 ± 0.2 (P < 0.05). During thermorresistance test...(AU)

Relações entre a câmara de Neubauer a espectrofotometria utilizadas para a determinação da concentração espermática de catetos (Pecari tajacu) / Relationship between Neubauer counting chamber and spectrophotometer used for the sperm concentration determination in collared peccaries (Pecari tajacu)

Em ejaculados provenientes de 28 catetos, verificou-se a existência de relações entre a concentração espermática determinada por meio da câmara de Neubauer e a tramitância observada por espectrofotometria, utilizando comprimentos de onda variando de 530 a 590nm. Os ejaculados apresentaram uma concentração média de 283,9±30,8x106 espermatozoides mL-1, com variação de 30 a 640x106 espermatozoides mL-1. Os valores para tramitância variaram entre 36,9 a 96,3, nos diferentes comprimentos de onda. Não foram detectadas relações significativas entre os dois métodos (P>0,05). Dessa forma, não se recomenda a espectrofotometria para os procedimentos de rotina na determinação da concentração espermática em catetos.(AU)

In ejaculates derived from 28 collared peccaries, we verified the existence of relationships between sperm concentration determined by the Neubauer counting chamber and the tramitance verified by spectrophotometer, under wavelengths varying from 530 to 590nm. Ejaculates presented a concentration of 283.9±30.8x106sperm m-1, varying from 30 to 640x106sperm mL-1. Values for tramitance varied from 36.9 to 96.3, under different wavelengths. No significant relationship was verified between two methods (P>0.05). Thus, the spectrophotometer is not recommended for routine procedures of sperm concentration measurement in collared peccaries.(AU)

Survival of collared peccaries (Pecari tajacu) sperm after freezing-thawing using different extenders / Sobrevivência de espermatozoides de catetos (Pecari tajacu) após congelação-descongelação no uso de diferentes diluentes

Background: The development of semen cryopreservation protocols for collared peccaries (Pecari tajacu) would contribute to its preservation and multiplication. Nowadays, studies on this subject report the use of Tris and powdered coconutwater (ACP®-116c) as extenders. However, such studies are limited to the post-thawing immediate evaluation of samples.The knowledge on the sperm survival after thawing would be important in order to schedule the moment and the vial forartificial insemination. Therefore, the aim of the present research was to verify the effect of Tris and ACP extenders on thesperm survival of frozen-thawed semen, centrifuged or not, derived from collared peccaries.Materials, Methods & Results: Semen from 12 adult males collared peccaries were obtained by electroejaculation. Ejaculateswere immediately evaluated for sperm motility, vigor, viability, functional membrane integrity and morphology. Sampleswere diluted in Tris or ACP, both plus 10% egg yolk and 3% glycerol. Then, they were packaged into 0.25 mL straws, andstored in liquid nitrogen. After one week, samples were thawed at 38ºC/1 min and divided into two aliquots: one immediately diluted in the same extender, and the other submitted to centrifugation and then re-diluted. A thermorresistance testwas conducted including assessments at 0, 15, 30, 45, and 60 min as described for fresh semen. After analyzing the dataimmediately after thawing, we verify that coconut water provided a better preservation of sperm motility (41.3 ± 6.9%)and vigor (2.9 ± 0.2) than Tris, 33.1 ± 5.9% motile sperm with vigor 2.5 ± 0.2 (P < 0.05). During thermorresistance test...