Percutaneous application of allogeneic adipose-derived mesenchymal stem cell in dogs submitted to minimally invasive plate osteosynthesis of the tibia

Purpose To evaluate clinical outcome following minimally invasive plate osteosynthesis (MIPO) associated with percutaneous transplantation of allogeneic adipose-derived mesenchymal stem cells (AD-MSC) at the tibial fracture site in dogs. Methods Thirty-six dogs presenting with nonarticular complete tibial fracture were included in this study. All fractures were treated by the same MIPO technique. The animals were divided in group 1 (n = 20) received a percutaneous application of 3 × 106 AD-MSC at the fracture site and group 2 (n = 16) did not receive any adjuvant treatment. Postoperative radiographic examinations were made at 15, 30, 60, 90 and 120 days. Results Fifty-eight percent of the patients were classified as skeletally immature. The median weight of the animals was 18.8 kg. The mean radiographic union time differed statistically between the AD-MSC group (28.5 days) and the control group (70.3 days). Sixty percent of dogs in group 1 and 56.25% of the group 2 were considered immature. Conclusions The use of allogeneic AD-MSC cell therapy and MIPO is a safe, viable and effective technique for promoting bone healing in nonarticular tibial fractures in dogs.(AU)

Comparação de três métodos de imagem para análise da evolução da osteoartrite induzida pela ruptura do ligamento cranial em coelhos / Comparison of three imaging methods for the evaluation of osteoarthritis induced by cranial cruciate ligament transection in rabbits

Background: Osteoarthritis is a degenerative joint disease that affects specially cartilage, meniscus, and tendons. Ligaments, muscles, subchondral bone and synovium. This pathology is a common condition limiting the quality of life ofpatients. Imaging modalities have also been used for evaluation the progression of the osteoarthritis, or degenerative processes induced by acute injury. In order to use more accessible imaging modalities for experimentation, this study aimedto compare radiographic, computed tomography, and ultrasound findings in the evaluation of osteoarthritis induced by thecranial cruciate ligament transection model in rabbits.Materials, Methods & Results: Twenty-four male Norfolk rabbits aged approximately 5 months old were used. All rabbits were submitted to cranial cruciate ligament transection of the left stifle and evaluated 45 days after the surgery. Theradiographic findings were subchondral bone sclerosis (33.33%); joint space narrowing (66%); presence of osteophytesat medial femoral condyle (4.16%), lateral femoral condyle (4.16%), medial fabela (20.83%), lateral fabela (8.33%) andsesamoid of the popliteal muscle (4.16%). No osteophytes were seen at medial and lateral tibial condyles. The tomographiccomputed findings were joint space narrowing (62.5%); presence of osteophytes at medial femoral condyle (75%), lateralfemoral condyle (54.16%), medial fabela (66.66%), lateral fabela (37.5%), medial tibial condyle (75%), lateral tibialcondyle (20.83%) and sesamoid of the popliteal muscle (37.5%). The ultrasound findings were synovial hypertrophy(95.83%); effusion in the suprapatellar recess (75%), distal tibial recess (16.66%) and cranial joint space (75%); changes(hyperechogenic foci and heterogeneity) of the lateral meniscus (50%) and medial meniscus (25%); increased thickness ofthe medial condyle (54.16%) and lateral condyle (45.83%); irregularity of the medial condyle (66.66%) and lateral condyle...

Comparação de três métodos de imagem para análise da evolução da osteoartrite induzida pela ruptura do ligamento cranial em coelhos / Comparison of three imaging methods for the evaluation of osteoarthritis induced by cranial cruciate ligament transection in rabbits

Background: Osteoarthritis is a degenerative joint disease that affects specially cartilage, meniscus, and tendons. Ligaments, muscles, subchondral bone and synovium. This pathology is a common condition limiting the quality of life ofpatients. Imaging modalities have also been used for evaluation the progression of the osteoarthritis, or degenerative processes induced by acute injury. In order to use more accessible imaging modalities for experimentation, this study aimedto compare radiographic, computed tomography, and ultrasound findings in the evaluation of osteoarthritis induced by thecranial cruciate ligament transection model in rabbits.Materials, Methods & Results: Twenty-four male Norfolk rabbits aged approximately 5 months old were used. All rabbits were submitted to cranial cruciate ligament transection of the left stifle and evaluated 45 days after the surgery. Theradiographic findings were subchondral bone sclerosis (33.33%); joint space narrowing (66%); presence of osteophytesat medial femoral condyle (4.16%), lateral femoral condyle (4.16%), medial fabela (20.83%), lateral fabela (8.33%) andsesamoid of the popliteal muscle (4.16%). No osteophytes were seen at medial and lateral tibial condyles. The tomographiccomputed findings were joint space narrowing (62.5%); presence of osteophytes at medial femoral condyle (75%), lateralfemoral condyle (54.16%), medial fabela (66.66%), lateral fabela (37.5%), medial tibial condyle (75%), lateral tibialcondyle (20.83%) and sesamoid of the popliteal muscle (37.5%). The ultrasound findings were synovial hypertrophy(95.83%); effusion in the suprapatellar recess (75%), distal tibial recess (16.66%) and cranial joint space (75%); changes(hyperechogenic foci and heterogeneity) of the lateral meniscus (50%) and medial meniscus (25%); increased thickness ofthe medial condyle (54.16%) and lateral condyle (45.83%); irregularity of the medial condyle (66.66%) and lateral condyle...(AU)

Uso do plasma rico em plaquetas como adjuvante no tratamento de lesão meniscal e tendinose de calcâneo em um cão / Use of platelet-rich plasma as an adjuvant on treatment of meniscal lesion and tendinosis of calcaneus in a dog

O objetivo deste trabalho é relatar o caso de um cão com lesão meniscal associado à tendinose de calcâneo tratado com aplicação única de plasma rico em plaquetas (PRP) intraarticular e intratendínea. Foi atendido um cão da raça Boxer com dificuldade de deambulação há três meses. Observou-se claudicação grau II/IV em ambos os membros pélvicos, atrofia muscular bilateral, dor e leve crepitação em joelho esquerdo, discreta instabilidade aos testes de gaveta e tibial cranial, com intensa fibrose medial à articulação e espessamento/rigidez do tendão calcâneo direito. O estudo radiográfico demonstrou artrose moderada no joelho esquerdo e, ao exame ultrassonográfico, constatou-se áreas de hipoecogenicidade em menisco medial esquerdo e ruptura de fibras musculares do tendão calcâneo direito. Sendo assim, o paciente foi diagnosticado com insuficiência do ligamento cruzado cranial crônica, com estabilidade fibrótica periarticular, e lesão meniscal em joelho esquerdo e tendinose de calcâneo direito. Foram prescritos condroprotetores, diacereína, fisioterapia e uma aplicação de PRP intra-articular e intratendínea. Para a concentração do PRP, foram coletados 50mL de sangue autólogo, os quais foram depositados em frascos contendo citrato de sódio. Foi realizada dupla centrifugação, sendo a primeira com força de120g e a segunda 240g, ambas por dez minutos. Ao final, obteve-se 2mL de PRP com246.000 plaquetas/µL (antes da concentração o sangue tinha 141.000 plaquetas/µL). A aplicação ocorreu de forma asséptica, sob anestesia geral e guiada por ultrassom. Foram injetados 1ml intra-articular em joelho esquerdo, 0,25mL em região perilesional e 0,75mLintralesional em tendão calcâneo direito. Após 14 dias, notou-se grau de claudicação I/IV e discreto fortalecimento muscular. Foi mantido o tratamento clínico com fisioterapia e condroprotetores com retornos periódicos para acompanhamento da evolução. Conclui-se que o uso do PRP foi importante adjuvante na terapia clínica empregada.

The aim of this paper is to report the case of a dog with meniscal lesion associated with calcaneal tendinosis treated with single application of intra-articular and intratendinous platelet rich plasma (PRP). A dog of the Boxer racer with difficulty walking was treated three months ago. Grade II/IV lameness was observed in both pelvic limbs, bilateral muscle atrophy, pain and slight crepitation in the left knee, slight instability to the drawer and cranial tibial tests, with intense medial fibrosis to the joint and right calcaneal tendon thickening/stiffness. The radiographic study showed moderate arthrosis in the left knee, and ultrasound examination revealed areas of hypoechogenicity in the left medial meniscus and rupture of the right calcaneal tendon muscle fibers. Thus, the patient was diagnosed with chronic cranial cruciate ligament insufficiency, periarticular fibrotic stability, and left meniscal injury and right calcaneal tendinosis. Chondroprotectors, diacerein, physiotherapyand an application of intra-articular and intratendinous PRP were prescribed. For the PRP concentration, 50mL of autologous blood were collected and deposited in vials containing sodium citrate. A double centrifugation was performed, the first strength of 120g and the second 240g, both for ten minutes. At the end, 2mL of PRP was obtained with 246.000platelets/µL (before concentration the blood had 141.000 platelets/µL). The application occurred aseptically, under general anesthesia and guided by ultrasound. We injected 1mlintra-articular in the left knee, 0.25mL in the perilesional region and 0.75mL intralesional in the right calcaneal tendon. After 14 days, I/IV lameness and slight muscle strengthening were noted. Clinical treatment with physiotherapy and chondroprotectors was maintained, with periodic returns to follow the evolution. It was concluded that the use of PRP was an important adjuvant in the clinical therapy employed.

Uso do plasma rico em plaquetas como adjuvante no tratamento de lesão meniscal e tendinose de calcâneo em um cão / Use of platelet-rich plasma as an adjuvant on treatment of meniscal lesion and tendinosis of calcaneus in a dog

O objetivo deste trabalho é relatar o caso de um cão com lesão meniscal associado à tendinose de calcâneo tratado com aplicação única de plasma rico em plaquetas (PRP) intraarticular e intratendínea. Foi atendido um cão da raça Boxer com dificuldade de deambulação há três meses. Observou-se claudicação grau II/IV em ambos os membros pélvicos, atrofia muscular bilateral, dor e leve crepitação em joelho esquerdo, discreta instabilidade aos testes de gaveta e tibial cranial, com intensa fibrose medial à articulação e espessamento/rigidez do tendão calcâneo direito. O estudo radiográfico demonstrou artrose moderada no joelho esquerdo e, ao exame ultrassonográfico, constatou-se áreas de hipoecogenicidade em menisco medial esquerdo e ruptura de fibras musculares do tendão calcâneo direito. Sendo assim, o paciente foi diagnosticado com insuficiência do ligamento cruzado cranial crônica, com estabilidade fibrótica periarticular, e lesão meniscal em joelho esquerdo e tendinose de calcâneo direito. Foram prescritos condroprotetores, diacereína, fisioterapia e uma aplicação de PRP intra-articular e intratendínea. Para a concentração do PRP, foram coletados 50mL de sangue autólogo, os quais foram depositados em frascos contendo citrato de sódio. Foi realizada dupla centrifugação, sendo a primeira com força de120g e a segunda 240g, ambas por dez minutos. Ao final, obteve-se 2mL de PRP com246.000 plaquetas/µL (antes da concentração o sangue tinha 141.000 plaquetas/µL). A aplicação ocorreu de forma asséptica, sob anestesia geral e guiada por ultrassom. Foram injetados 1ml intra-articular em joelho esquerdo, 0,25mL em região perilesional e 0,75mLintralesional em tendão calcâneo direito. Após 14 dias, notou-se grau de claudicação I/IV e discreto fortalecimento muscular. Foi mantido o tratamento clínico com fisioterapia e condroprotetores com retornos periódicos para acompanhamento da evolução. Conclui-se que o uso do PRP foi importante adjuvante na terapia clínica empregada.(AU)

The aim of this paper is to report the case of a dog with meniscal lesion associated with calcaneal tendinosis treated with single application of intra-articular and intratendinous platelet rich plasma (PRP). A dog of the Boxer racer with difficulty walking was treated three months ago. Grade II/IV lameness was observed in both pelvic limbs, bilateral muscle atrophy, pain and slight crepitation in the left knee, slight instability to the drawer and cranial tibial tests, with intense medial fibrosis to the joint and right calcaneal tendon thickening/stiffness. The radiographic study showed moderate arthrosis in the left knee, and ultrasound examination revealed areas of hypoechogenicity in the left medial meniscus and rupture of the right calcaneal tendon muscle fibers. Thus, the patient was diagnosed with chronic cranial cruciate ligament insufficiency, periarticular fibrotic stability, and left meniscal injury and right calcaneal tendinosis. Chondroprotectors, diacerein, physiotherapyand an application of intra-articular and intratendinous PRP were prescribed. For the PRP concentration, 50mL of autologous blood were collected and deposited in vials containing sodium citrate. A double centrifugation was performed, the first strength of 120g and the second 240g, both for ten minutes. At the end, 2mL of PRP was obtained with 246.000platelets/µL (before concentration the blood had 141.000 platelets/µL). The application occurred aseptically, under general anesthesia and guided by ultrasound. We injected 1mlintra-articular in the left knee, 0.25mL in the perilesional region and 0.75mL intralesional in the right calcaneal tendon. After 14 days, I/IV lameness and slight muscle strengthening were noted. Clinical treatment with physiotherapy and chondroprotectors was maintained, with periodic returns to follow the evolution. It was concluded that the use of PRP was an important adjuvant in the clinical therapy employed.(AU)

Acoustic radiation force impulse (ARFI) elastography of the stifle joint of healthy beagles / [Elastografia acoustic radiation force impulse (ARFI) da articulação do joelho de beagles saudáveis]

The objective was to establish the reference standards for elastography of the main structures of the canine stifle joint. The medial meniscus, patellar and cruciate ligaments of thirty healthy beagles was evaluated by B-mode and ARFI elastography (n=60 joints). Analysis detected a positive correlation of the shear wave velocity (SWV) of structures according to age. Patellar ligament presented a gradual stiffness increase in relation to age, while medial meniscus and cruciate ligament were more rigid in puppies than in adults. However, greater stiffness of these structures was observed in elderly animals. Elasticity of each structure was evaluated according to gender and reproductive status. Females presented greater stiffness of all structures, with SWV differing 0.3 and 0.36m/s between genders. Only the medial meniscus was not stiffer in neutered animals when compared to intact animals, differing only 0.02m/s in menisci and 0.4 to 0.47m/s in ligaments between groups. These findings corroborate with literature data that states a higher prevalence of ligament insufficiency in elderly dogs, females and neutered animals. It was concluded that ARFI elastography of the canine stifle joint is feasible and its application can be potentially effective in early diagnosis of ligament and meniscal changes.(AU)

O objetivo foi estabelecer os padrões normais para elastografia das principais estruturas da articulação do joelho em cães. O menisco medial, ligamento patelar e cruzado de trinta beagles saudáveis foram avaliados pelo modo B e elastografia ARFI (n=60 articulações). A análise detectou uma correlação positiva da velocidade de cisalhamento (SWV) das estruturas com a idade. O ligamento patelar apresentou um aumento gradual da rigidez em relação à idade, enquanto o menisco medial e o ligamento cruzado foram mais rígidos em filhotes do que em adultos. Entretanto, observou-se maior rigidez dessas estruturas em idosos. A elasticidade de cada estrutura foi avaliada de acordo com o sexo e o estado reprodutivo. As fêmeas apresentaram maior rigidez em todas as estruturas, com SWV diferente de 0,3 e 0,36m/s entre os sexos. Somente o menisco medial não foi mais rígido nos animais castrados quando comparado não castrados, diferindo apenas 0,02m/s no menisco e 0,4 a 0,47m/s nos ligamentos entre os grupos. Esses achados corroboram com dados da literatura que afirmam maior prevalência de insuficiência ligamentar em cães idosos, fêmeas e animais castrados. Concluiu-se que a elastografia ARFI da articulação do joelho canino é viável e sua aplicação pode ser eficaz no diagnóstico precoce de alterações ligamentares e meniscais.(AU)

Isolated lateral meniscus rupture in dogs: case report / Ruptura isolada de menisco lateral em cão – relato de caso

Menisci are important intra-articular structures whose main function is attributed to load transmission and impact absorption, reducing biomechanical stress and providing greater osteoarticular stability. In dogs, knee-related disorders are described as the main causes of claudication. This report aims to describe a case of lateral meniscal lesion in a canine without signs of femorotibiopatellar instability, whose main change was claudication. The diagnosis was confirmed through orthopedic evaluation and magnetic resonance imaging, in which an anechogenic line of dissolution of meniscal continuity and intact ligament structures was visualized. As a treatment, the technique of total meniscectomy was chosen, which allowed the recovery of limb function and absence of clinical manifestations.

Os meniscos são importantes estruturas intra-articulares cuja principal função atribui-se à transmissão de carga e à absorção de impacto, diminuindo o estresse biomecânico e conferindo maior estabilidade osteoarticular. Em cães, as afecções relacionadas aojoelho são descritas como as principais causas de claudicação. O presente relato tem como objetivo descrever um caso de lesão meniscal lateral em um canino sem sinais de instabilidade femorotibiopatelar, cuja principal alteração era claudicação. O diagnóstico foi confirmado através da avaliação ortopédica e exame de ressonância magnética, no qual foi visualizada uma linha anecogênica de dissolução de continuidade meniscal e estruturas ligamentares íntegras. Como tratamento, optou-se pela técnica de meniscectomia total, o que possibilitou o retorno da função do membro e ausência de manifestações clínicas.

Isolated lateral meniscus rupture in dogs: case report / Ruptura isolada de menisco lateral em cão relato de caso

Menisci are important intra-articular structures whose main function is attributed to load transmission and impact absorption, reducing biomechanical stress and providing greater osteoarticular stability. In dogs, knee-related disorders are described as the main causes of claudication. This report aims to describe a case of lateral meniscal lesion in a canine without signs of femorotibiopatellar instability, whose main change was claudication. The diagnosis was confirmed through orthopedic evaluation and magnetic resonance imaging, in which an anechogenic line of dissolution of meniscal continuity and intact ligament structures was visualized. As a treatment, the technique of total meniscectomy was chosen, which allowed the recovery of limb function and absence of clinical manifestations.(AU)

Os meniscos são importantes estruturas intra-articulares cuja principal função atribui-se à transmissão de carga e à absorção de impacto, diminuindo o estresse biomecânico e conferindo maior estabilidade osteoarticular. Em cães, as afecções relacionadas aojoelho são descritas como as principais causas de claudicação. O presente relato tem como objetivo descrever um caso de lesão meniscal lateral em um canino sem sinais de instabilidade femorotibiopatelar, cuja principal alteração era claudicação. O diagnóstico foi confirmado através da avaliação ortopédica e exame de ressonância magnética, no qual foi visualizada uma linha anecogênica de dissolução de continuidade meniscal e estruturas ligamentares íntegras. Como tratamento, optou-se pela técnica de meniscectomia total, o que possibilitou o retorno da função do membro e ausência de manifestações clínicas.(AU)

Morfometria dos meniscos lateral e medial na articulação femorotibial de equinos / Morphometry of the lateral and medial meniscus in the equine femorotibial joint

As lesões dos meniscos são uma das principais causas de dor e claudicação em equinos. O objetivo do presente estudo foi avaliar a morfometria dos meniscos da articulação femorotibial de equinos. Foram utilizados 48 meniscos de 12 animais de ambos os sexos, com idade entre cinco e 15 anos e com peso entre 400kg e 500kg. A medida da extensão periférica compreendeu desde a parte mais cranial à mais caudal e denominou-se circunferência externa (CE). A margem interna, com o mesmo tratamento, foi chamada de circunferência interna (CI). Os meniscos foram divididos em terços craniais, médios e caudais. A espessura foi obtida nos pontos médios de cada terço. Foi calculada a área dos meniscos em contato com os côndilos femorais. O menisco medial apresentou maior CE com média de 126,38mm, enquanto o menisco lateral apresentou média de 115,32mm. O menisco lateral mostrou maior espessura nos terços médio e caudal, com valores médios de 16,00mm e 19,85mm, respectivamente, contra 13,75mm e 14,99mm dos meniscos mediais. Os resultados deste estudo mostraram relação importante entre os dados morfométricos e os achados clínicos na tentativa de explicar a maior incidência de lesões envolvendo o menisco medial.(AU)

Meniscal lesions are one of the main causes of pain and lameness in horses. The objective of this study was to evaluate the morphometry of the meniscus of the femorotibial joint of horses. 48 meniscus were used from 12 animals, aged between five and 15 years and weighing between 400kg and 500kg. The measurement of the peripheral extension went from the most cranial part to the most caudal, denominated external circumference (CE). The internal margin, with the same treatment, was called internal circumference (IC). The meniscus was divided into cranial, middle and caudal thirds. The thickness was obtained at the midpoints of each third. The area of the meniscus in contact with the femoral condyles was also calculated. The medial meniscus presented a higher CE with a mean of 126.38mm, while the lateral meniscus presented a mean of 115.32mm. The lateral meniscus showed greater thickness in the middle and caudal thirds, with mean values of 16.00mm and 19.85mm respectively, against 13.75mm and 14.99mm of the medial meniscus. The results of this study showed an important relationship between the morphometric data and the clinical findings in an attempt to explain the higher incidence of lesions involving the medial meniscus.(AU)

Morfometria dos meniscos lateral e medial na articulação femorotibial de equinos / Morphometry of the lateral and medial meniscus in the equine femorotibial joint

As lesões dos meniscos são uma das principais causas de dor e claudicação em equinos. O objetivo do presente estudo foi avaliar a morfometria dos meniscos da articulação femorotibial de equinos. Foram utilizados 48 meniscos de 12 animais de ambos os sexos, com idade entre cinco e 15 anos e com peso entre 400kg e 500kg. A medida da extensão periférica compreendeu desde a parte mais cranial à mais caudal e denominou-se circunferência externa (CE). A margem interna, com o mesmo tratamento, foi chamada de circunferência interna (CI). Os meniscos foram divididos em terços craniais, médios e caudais. A espessura foi obtida nos pontos médios de cada terço. Foi calculada a área dos meniscos em contato com os côndilos femorais. O menisco medial apresentou maior CE com média de 126,38mm, enquanto o menisco lateral apresentou média de 115,32mm. O menisco lateral mostrou maior espessura nos terços médio e caudal, com valores médios de 16,00mm e 19,85mm, respectivamente, contra 13,75mm e 14,99mm dos meniscos mediais. Os resultados deste estudo mostraram relação importante entre os dados morfométricos e os achados clínicos na tentativa de explicar a maior incidência de lesões envolvendo o menisco medial.(AU)

Meniscal lesions are one of the main causes of pain and lameness in horses. The objective of this study was to evaluate the morphometry of the meniscus of the femorotibial joint of horses. 48 meniscus were used from 12 animals, aged between five and 15 years and weighing between 400kg and 500kg. The measurement of the peripheral extension went from the most cranial part to the most caudal, denominated external circumference (CE). The internal margin, with the same treatment, was called internal circumference (IC). The meniscus was divided into cranial, middle and caudal thirds. The thickness was obtained at the midpoints of each third. The area of the meniscus in contact with the femoral condyles was also calculated. The medial meniscus presented a higher CE with a mean of 126.38mm, while the lateral meniscus presented a mean of 115.32mm. The lateral meniscus showed greater thickness in the middle and caudal thirds, with mean values of 16.00mm and 19.85mm respectively, against 13.75mm and 14.99mm of the medial meniscus. The results of this study showed an important relationship between the morphometric data and the clinical findings in an attempt to explain the higher incidence of lesions involving the medial meniscus.(AU)

Tear meniscometry test in wild animals / Teste de meniscometria lacrimal em animais silvestres

This study aimed to describe strip meniscometry test values for Amazona aestiva (turquoise-fronted amazon), Ara ararauna (blue-and-yellow macaw) and Caiman latirostris (broad-snouted caiman). Twenty-one Amazona aestiva and 11 Ara ararauna, healthy, adults of unknown sex, and 37 healthy adult Caiman latirostris (27 males and 10 females), were used in this investigation. All animals were manually restrained and I-Tear® Test strip was inserted for 5 s at the edge of the lower tear meniscus, without touching the eyelid or ocular surface, immediately followed by measurement (millimeter per 5 seconds). No differences were reported between left and right eyes for Amazona aestiva (P = 0.824), Ara ararauna (P = 0.262) and Caiman latirostris (P = 0.679). Median and confidence interval were 2.0 (2.25-3.56) mm/5s for Amazona aestiva, 3.0 (2.93-4.15) mm/5s for Ara ararauna and 3.0 (2.55-4.90) mm/5 s for Caiman latirostris. These values for strip meniscometry tear test can be used as parameters for each species and may be helpful for diagnosis and treatment of tear-production disorders in birds and reptiles.(AU)

O objetivo deste estudo foi descrever os valores do teste de meniscometria lacrimal para Amazona aestiva (papagaio-verdadeiro), Ara ararauna (arara-canindé) e Caiman latirostris (jacaré-de-papo-amarelo). Vinte e um Amazona aestiva, 11 Ara ararauna adultos, de sexo desconhecido, e 37 Caiman latirostris adultos (27 machos e 10 fêmeas), foram utilizados nesta investigação. Todos os animais foram contidos manualmente e uma tira do I-Tear® test foi inserida por 5 segundos na borda do menisco inferior, sem tocar a pálpebra ou a superfície ocular, e imediatamente mensurada (milímetro por 5 segundos). Não foram encontradas diferenças entre os olhos esquerdos e direitos para Amazona aestiva (P = 0,824), Ara ararauna (P = 0,262) e Caiman latirostris (P = 0,679). Mediana e intervalo de confiança foram de 2,0 (2,25-3,56) mm/5 s para Amazona aestiva, 3,0 (2,93-4,15) mm/5 s para Ara ararauna e 3,0 (2,55-4,90) mm/5 s para Caiman latirostris. Estes valores para o teste de meniscometria podem ser utilizados como parâmetros para estas espécies e podem ser úteis para diagnosticar e tratar distúrbios de produção lacrimal em aves e répteis.(AU)

Avaliação ultrassonográfica de cães com ruptura do ligamento cruzado cranial submetidos a artrotomia / Ultrasonographic evaluation of dogs with cranial cruciate ligament rupture undergoing to arthrotomy

Background: The cranial cruciate ligament rupture (CrCLR) is the most common disease of the stifle joint in dogs. Oneof the major concerns in the assessment of these animals is diagnosing the presence of a medial meniscus tears, which isa frequent consequence due to the instability of the joint. Ultrasonography is indicated in the evaluation of the meniscifrom human and canine patients, and the results are similar to magnetic resonance imaging. The aim of this study was tocompare the ultrasonography and the arthrotomy in the evaluation of the cranial cruciate ligament and medial meniscusof dogs with CrCLR undergoing surgery for stifle stabilization.Materials, Methods & Results: Ultrasonographic examination was performed prior to arthrotomy in 23 dogs with diagnosis of complete CrCLR and five with suspected partial CrCLR undergoing for stifle joint stabilization surgery. Theultrasonography identified the complete rupture in 82.6% and the arthrotomy in 100% of the joints with this diagnosis. Inthe joints with suspected CrCLR during the clinical exam, arthrotomy and ultrasonography identified respectively threeand four joints with partial CrCLR, and two and one with healthy ligaments. There was no difference between the twotechniques in the assessment of the cranial cruciate ligament (P = 0.20). The ultrasonography identified medial meniscaltears in 39.3%, while arthrotomy was found in 21.4% (P = 0.0006) of the joints. The most frequent meniscal tear type observed in the arthrotomy was folded caudal horn. The ultrasonography was able only in differentiate presence and absenceof meniscal injury. Besides the real tear that affects the meniscus morphology, the ultrasound also identified echogenicityand echotexture changes in the medial (5/28) and lateral (8/28) menisci. Other changes observed in all joints evaluated byultrasound were the presence of effusion and synovial membrane thickening...

Avaliação ultrassonográfica de cães com ruptura do ligamento cruzado cranial submetidos a artrotomia / Ultrasonographic evaluation of dogs with cranial cruciate ligament rupture undergoing to arthrotomy

Background: The cranial cruciate ligament rupture (CrCLR) is the most common disease of the stifle joint in dogs. Oneof the major concerns in the assessment of these animals is diagnosing the presence of a medial meniscus tears, which isa frequent consequence due to the instability of the joint. Ultrasonography is indicated in the evaluation of the meniscifrom human and canine patients, and the results are similar to magnetic resonance imaging. The aim of this study was tocompare the ultrasonography and the arthrotomy in the evaluation of the cranial cruciate ligament and medial meniscusof dogs with CrCLR undergoing surgery for stifle stabilization.Materials, Methods & Results: Ultrasonographic examination was performed prior to arthrotomy in 23 dogs with diagnosis of complete CrCLR and five with suspected partial CrCLR undergoing for stifle joint stabilization surgery. Theultrasonography identified the complete rupture in 82.6% and the arthrotomy in 100% of the joints with this diagnosis. Inthe joints with suspected CrCLR during the clinical exam, arthrotomy and ultrasonography identified respectively threeand four joints with partial CrCLR, and two and one with healthy ligaments. There was no difference between the twotechniques in the assessment of the cranial cruciate ligament (P = 0.20). The ultrasonography identified medial meniscaltears in 39.3%, while arthrotomy was found in 21.4% (P = 0.0006) of the joints. The most frequent meniscal tear type observed in the arthrotomy was folded caudal horn. The ultrasonography was able only in differentiate presence and absenceof meniscal injury. Besides the real tear that affects the meniscus morphology, the ultrasound also identified echogenicityand echotexture changes in the medial (5/28) and lateral (8/28) menisci. Other changes observed in all joints evaluated byultrasound were the presence of effusion and synovial membrane thickening...(AU)

Anatomia microscópica e ultraestrutura do joelho da paca (Cuniculus paca Linnaeus, 1766) / Microscopic anatomy and ultrastructure of paca stifle (Cuniculus paca Linnaeus, 1766)

A paca (Cuniculus paca), um dos maiores roedores da fauna brasileira, possui características inerentes à sua espécie que podem contribuir como uma nova opção de animal experimental; assim, considerando-se que há crescente busca por modelos experimentais apropriados para ortopedia e pesquisas cirúrgicas, foram analisados e descritos em detalhes a anatomia microscópica e ultraestrutural do joelho desse roedor. Os ligamentos colaterais são constituídos por feixes de fibras colágenas arranjadas paralelamente e com trajeto ondulado. Os fibroblastos formavam fileiras paralelas às fibras colágenas; quanto aos ligamentos colaterais, estes apresentaram citoplasma imperceptível à avaliação por microscopia de luz, entretanto, em análise ultraestrutural verificou-se vários prolongamentos citoplasmáticos. Microscopicamente, as estruturas presentes no joelho da paca assemelham-se às dos animais domésticos, roedores e lagomorfos.(AU)

Paca (Cuniculus paca), one of the largest rodent of the Brazilian fauna, has characteristics inherent to the species that can contribute as a new experimental animal; so, considering that there is a growing search for experimental models suitable for orthopedic and surgical research, it was analyzed and described in detail the microscopic and ultrastructural anatomy of the stifle in this rodent. The collateral ligaments are composed of bundles of collagen fibers arranged in parallel and in wavy path. Fibroblasts formed parallel rows to the collagen fibers; concerning the collateral ligaments, they presented imperceptible cytoplasm at light microscopy, but at ultrastructure analysis they presented several cytoplasmic processes. At the microscopic level, the stifle of paca resembles the domestic animals, rodents and lagomorphs.(AU)

Anatomia microscópica e ultraestrutura do joelho da paca (Cuniculus paca Linnaeus, 1766) / Microscopic anatomy and ultrastructure of paca stifle (Cuniculus paca Linnaeus, 1766)

A paca (Cuniculus paca), um dos maiores roedores da fauna brasileira, possui características inerentes à sua espécie que podem contribuir como uma nova opção de animal experimental; assim, considerando-se que há crescente busca por modelos experimentais apropriados para ortopedia e pesquisas cirúrgicas, foram analisados e descritos em detalhes a anatomia microscópica e ultraestrutural do joelho desse roedor. Os ligamentos colaterais são constituídos por feixes de fibras colágenas arranjadas paralelamente e com trajeto ondulado. Os fibroblastos formavam fileiras paralelas às fibras colágenas; quanto aos ligamentos colaterais, estes apresentaram citoplasma imperceptível à avaliação por microscopia de luz, entretanto, em análise ultraestrutural verificou-se vários prolongamentos citoplasmáticos. Microscopicamente, as estruturas presentes no joelho da paca assemelham-se às dos animais domésticos, roedores e lagomorfos.(AU)

Paca (Cuniculus paca), one of the largest rodent of the Brazilian fauna, has characteristics inherent to the species that can contribute as a new experimental animal; so, considering that there is a growing search for experimental models suitable for orthopedic and surgical research, it was analyzed and described in detail the microscopic and ultrastructural anatomy of the stifle in this rodent. The collateral ligaments are composed of bundles of collagen fibers arranged in parallel and in wavy path. Fibroblasts formed parallel rows to the collagen fibers; concerning the collateral ligaments, they presented imperceptible cytoplasm at light microscopy, but at ultrastructure analysis they presented several cytoplasmic processes. At the microscopic level, the stifle of paca resembles the domestic animals, rodents and lagomorphs.(AU)

Síntese e caracterização de nanopartículas de ouro conjugadas com curcumina e seus efeitos na osteoartrite experimental induzida / Synthesis and characterization of gold nanoparticles combined with curcumin and its effects on experimentally induced osteoarthritis

A Osteoartrite (OA) é uma denominação clínica para uma combinação de condições patológicas que envolvem a degeneração progressiva da cartilagem articular e remodelação de osso subcondral. A curcumina, um potente agente anti-inflamatório, têm sido extensivamente estudada, no entanto não oferece boa biodisponibilidade sistêmica. Nanopartículas de ouro (AuNPs) apresentam aplicações potenciais na administração de substâncias terapêuticas aumentando a eficiência do transporte de fármacos. O objetivo deste estudo foi realizar a síntese e caracterização de um sistema conjugando as AuNPs à curcumina e avaliar seu potencial terapêutico em um modelo experimental de OA em camundongos por desestabilização do menisco medial (DMM). As AuNPs foram conjugadas com curcumina e os sistemas foram caracterizados por espectroscopia no UV-VIS, espalhamento de luz dinâmico (DLS) e determinação do potencial zeta. Formou-se 4 grupos de oito animais cada, denominados A, B, C, D que receberam injeção intra-articular de AuNPs, curcumina, AuNP-curcumina e solução fisiológica, respectivamente. Após 7 semanas, a cartilagem da articulação-femoro- tibio-patelar (AFTP) foi avaliada em uma variação de escore de 0 a 24. A conjugação de AuNP-curcumina mostrou boa estabilidade e aplicação terapêutica, mas não diferiu significativamente (P>0,05) dos grupos A e B, no entanto, mostrou menor valor de escore e significância (P<0,001) em relação ao grupo controle. Os resultados deste trabalho mostram a importância do desenvolvimento de novos nanofármacos. Neste caso a conjugação de AuNPs com a curcumina permitiu a obtenção de um nanofármaco com sugestivo potencial para aplicação no tratamento da OA.

Osteoarthritis (OA) is the clinical term for a combination of pathological conditions that involve the progressive degeneration of articular cartilage and subchondral bone remodelling. Curcumin, a potent anti-inflammatory agent, has been extensively studied; however, it does not provide good systemic bioavailability. Gold nanoparticles (AuNPs) have potential applications in the administration of therapeutic substances in order to increase the transport efficiency of drugs. The objectives of this study were to explore the synthesis and characterization of a system combining AuNPs with curcumin and evaluate its therapeutic potential in an experimental model of OA in mice by the destabilization of the medial meniscus (DMM). The AuNPs were conjugated with curcumin and the systems were characterized by UV-VIS spectroscopy, dynamic light scattering (DLS), and zeta potential. Four groups of eight animals each were formed and labelled A, B, C, and D, which received intra-articular injections of AuNPs, curcumin, AuNP-curcumin, and physiologic solution, respectively. After seven weeks, the cartilage of the stifle joint (SJ) was rated on a scale ranging from 0 to 24. Combination of AuNP-curcumin demonstrated good stability and therapeutic applications, but it did not differ significantly (P>0.05) from groups A and B. However, the control group had a significantly lower score (P<0.001). Results of this study demonstrated the importance of developing new nanodrugs. In this case, the combination of AuNPs and curcumin yielded the nanodrug effects suggestive of a potential for application in the treatment of OA.

Síntese e caracterização de nanopartículas de ouro conjugadas com curcumina e seus efeitos na osteoartrite experimental induzida / Synthesis and characterization of gold nanoparticles combined with curcumin and its effects on experimentally induced osteoarthritis

A Osteoartrite (OA) é uma denominação clínica para uma combinação de condições patológicas que envolvem a degeneração progressiva da cartilagem articular e remodelação de osso subcondral. A curcumina, um potente agente anti-inflamatório, têm sido extensivamente estudada, no entanto não oferece boa biodisponibilidade sistêmica. Nanopartículas de ouro (AuNPs) apresentam aplicações potenciais na administração de substâncias terapêuticas aumentando a eficiência do transporte de fármacos. O objetivo deste estudo foi realizar a síntese e caracterização de um sistema conjugando as AuNPs à curcumina e avaliar seu potencial terapêutico em um modelo experimental de OA em camundongos por desestabilização do menisco medial (DMM). As AuNPs foram conjugadas com curcumina e os sistemas foram caracterizados por espectroscopia no UV-VIS, espalhamento de luz dinâmico (DLS) e determinação do potencial zeta. Formou-se 4 grupos de oito animais cada, denominados A, B, C, D que receberam injeção intra-articular de AuNPs, curcumina, AuNP-curcumina e solução fisiológica, respectivamente. Após 7 semanas, a cartilagem da articulação-femoro- tibio-patelar (AFTP) foi avaliada em uma variação de escore de 0 a 24. A conjugação de AuNP-curcumina mostrou boa estabilidade e aplicação terapêutica, mas não diferiu significativamente (P>0,05) dos grupos A e B, no entanto, mostrou menor valor de escore e significância (P<0,001) em relação ao grupo controle. Os resultados deste trabalho mostram a importância do desenvolvimento de novos nanofármacos. Neste caso a conjugação de AuNPs com a curcumina permitiu a obtenção de um nanofármaco com sugestivo potencial para aplicação no tratamento da OA.(AU)

Osteoarthritis (OA) is the clinical term for a combination of pathological conditions that involve the progressive degeneration of articular cartilage and subchondral bone remodelling. Curcumin, a potent anti-inflammatory agent, has been extensively studied; however, it does not provide good systemic bioavailability. Gold nanoparticles (AuNPs) have potential applications in the administration of therapeutic substances in order to increase the transport efficiency of drugs. The objectives of this study were to explore the synthesis and characterization of a system combining AuNPs with curcumin and evaluate its therapeutic potential in an experimental model of OA in mice by the destabilization of the medial meniscus (DMM). The AuNPs were conjugated with curcumin and the systems were characterized by UV-VIS spectroscopy, dynamic light scattering (DLS), and zeta potential. Four groups of eight animals each were formed and labelled A, B, C, and D, which received intra-articular injections of AuNPs, curcumin, AuNP-curcumin, and physiologic solution, respectively. After seven weeks, the cartilage of the stifle joint (SJ) was rated on a scale ranging from 0 to 24. Combination of AuNP-curcumin demonstrated good stability and therapeutic applications, but it did not differ significantly (P>0.05) from groups A and B. However, the control group had a significantly lower score (P<0.001). Results of this study demonstrated the importance of developing new nanodrugs. In this case, the combination of AuNPs and curcumin yielded the nanodrug effects suggestive of a potential for application in the treatment of OA.(AU)

Remote limb ischemic post-conditioning attenuates ischemia-reperfusion injury in rat skin flapby limiting oxidative stress

PURPOSE:To investigate the effect of remote ischemic post-conditioning (RIPoC) against ischemia-reperfusion (I/R) injury on flaps of rats.METHODS:Sprague-Dawley rats were randomized into the Sham, Control, RIPoC1 and RIPoC2 groups. All the animals were submitted to a 5×4 cm superficial inferior epigastric artery flap. Eight hours of flap ischemia was induced and two protocols of limb RIPoC were applied. Tissue MDA level and SOD activity in 24-h reperfusion were assessed. Flap survival was assessed 7 days postoperatively.RESULTS:Compared to the Control group, the RIPoC1 group showed statistically decreased MDA level at 6-, 12-, and 24-h reperfusion (P = 0.01, P < 0.01 and P < 0.01, respectively), and statistically increased SOD activity at 12- and 24-h reperfusion (P < 0.05 and P < 0.01, respectively). Flap survival rate on the 7th day was significantly higher in the RIPoC1 group than the control group (47.9 ± 6.4 vs . 29.4 ± 7.1 %, P < 0.01).CONCLUSION:Three cycles of 5-min Limb remote ischemic post-conditioning rather than a single cycle of 15-min limb RIPoC has protective effect on flaps against ischemia-reperfusion injury by attenuating oxidative stress.(AU)

RUPTURA DO LIGAMENTO CRUZADO CRANIAL EM GATOS: ESTUDO RETROSPECTIVO

A ruptura do ligamento cruzado cranial (LCCr) é uma afecção ainda pouco esclarecida nos gatos. Acredita-se que existam dois grupos distintos na espécie felina que podem manifestar lesão neste ligamento: no primeiro grupo, a ruptura do LCCr está associada ao fator traumático evidente; no segundo grupo, a lesão do LCCr está associada a fatores degenerativos. Este estudo teve o objetivo avaliar diversas características de gatos com ruptura do LCCr a fim de elucidar a afeção nesta espécie. Foi realizado estudo retrospectivo de 26 gatos com ruptura clínica do LCCr, os quais foram categorizados em dois grupos principais, levando em consideração o tipo de lesão presente (G1 e G2). O grupo G1 foi constituído por gatos que apresentaram como lesão a ruptura do LCCr associada a lesões traumáticas bem evidentes na articulação do joelho, e o grupo G2 constituído por gatos que apresentaram como lesão a ruptura do LCCr de forma isolada ou em associação com alteração em menisco. A partir desta divisão, foi realizada análise e verificação da associação estatística de G1 e G2 para as seguintes variáveis: fase de vida, escore corporal, comportamento indoor/outdoor, grau de claudicação, tipo de trauma e sinais de degeneração articular. Nas radiografias foram realizadas mensurações do ângulo do platô tibial (APT) e avaliados sinais de degeneração articular quando presentes, em estudo duplo cego. Foram obtidas amostras histológicas de estruturas da articulação do joelho de quatro gatos com ruptura do LCCr pertencente aos dois grupos (G1 e G2). Para análise estatística foram criadas tabelas de contingência e realizados teste de associação utilizando variáveis qualitativas nominais, Teste Qui-Quadrado de Partição e Teste Exato de Fisher. Foi observada associação significativa ((p 0,05) entre gatos com comportamento indoor, histórico traumático inexistente ou discreto e presença de sinais radiográficos de degeneração articular com o grupo que apresentou ruptura isolada do LCCr (G2). A mensuração do APT indicou que a ruptura do LCCr parece não ser influenciada pela inclinação do platô tibial nos gatos. Na avaliação histopatológica foram observadas características sugestivas de processo articular degenerativo. Conclui-se que alterações degenerativas podem estar relacionadas à etiologia da ruptura do LCCr em gatos.

Rupture of the cranial cruciate ligament (CrCL) is a condition still poorly clarified in cats. It is believed that there are two distinct groups in the feline species that can manifest an injury to the CrCL: in the first group, the rupture of the CrCL is associated to the evident traumatic factor; in the second group, the CrCL injury is associated with degenerative factors. This study aimed to evaluate several characteristics of cats with ruptured CrCL to elucidate the condition in this species. A retrospective study of 26 cats with clinical rupture of the CrCL was carried out, which were categorized into two main groups, taking into account the type of lesion present (G1 and G2). The G1 group consisted of cats that presented the CrCL rupture as an injury associated with evident traumatic injuries in the knee joint, and the G2 group consisted of cats that presented the CrCL rupture as an injury alone or in association with an alteration in meniscus. From this division, analysis and verification of the statistical association of G1 and G2 for the following variables was performed: life stage, body score, indoor/outdoor behavior, degree of lameness, type of trauma and signs of joint degeneration. In the radiographs, measurements of the tibial plateau angle (TPA) were performed, and signs of joint degeneration were evaluated, when present, in a double-blind study. Histological samples of knee joint structures were obtained from four cats with CrCL rupture belonging to the two groups (G1 and G2). For statistical analysis, contingency tables were created, and association test using nominal qualitative variables, Chi-Square Partition Test and Fisher's Exact Test were performed. A significant association (p 0.05) was observed between cats with indoor behavior, non-existent or mild traumatic history and the presence of radiographic signs of joint degeneration with the group that presented isolated rupture of the CrCL (G2). The measurement of the TPA indicated that the rupture of the CrCL does not seem to be influenced by the inclination of the tibial plateau in cats. In the histopathological evaluation, characteristics suggestive of a degenerative joint process were observed. It is concluded that degenerative changes may be related to the etiology of CrCL rupture in cats.

DETERMINAÇÃO DO POSICIONAMENTO DA CUNHA NA CTWO PELO MÉTODO DO CORA E APLICAÇÃO EM CÃES COM RUPTURA DE LIGAMENTO CRUZADO CRANIAL

Ante a alta frequência de ruptura de ligamento cruzado cranial (RLCCr) em cães, a osteotomia tibial em cunha cranial (CTWO) é uma das técnicas de osteotomia para reduzir o ângulo do platô tibial (APT) e assim anular o deslocamento cranial da tíbia proximal em relação aos côndilos femorais. A pesquisa teve como objetivo usar o método do centro de rotação angular (CORA) para posicionar a cunha da CTWO, realizando primeiro a avaliação gráfica da proposta e logo aplicando os resultados em cães acometidos com RLCCr. Após determinação do novo método de planejamento, foi feita a análise em 19 imagens radiográficas de tíbia de cães adultos normais para verificar quanto às mudanças do APT e encurtamento tibial com a modificação. Posteriormente, foram operados nove joelhos de sete cães com diagnóstico clínico e radiográfico de RLCCr, onde os fragmentos tibiais, proximal e distal, foram fixados com placa bloqueada e de compressão dinâmica de TPLO e banda de tensão, cuja cunha nunca ficou a menos de 5 mm distal à inserção do ligamento patelar e realizou-se em todos os casos o alinhamento dos fragmentos pela cortical cranial. A aplicação da técnica permitiu uma redução do APT médio de 24,04° para 7,74°. Verifica-se que a média do APT, segundo o alinhamento dos fragmentos entre as três categorias, variou de 7,73 a 8,03°, sem diferença significativa. As médias do APT, segundo a altura da cunha, variaram de 7,26° (5 mm abaixo da inserção do ligamento patelar), a 8,47° (na altura da distal) e de 7,74° quando a altura foi no CORA; a diferença se mostra significativa. O encurtamento ósseo médio de é 0,16 cm, que corresponde ao eixo funcional não considerado significativo para influenciar na recuperação clínica. Complicações menores, como fratura de fragmento, deiscência de ferida e lesão tradia de menisco, ocorreram em 30 % dos procedimentos, sem prejudicar a recuperação funcional, com mudança satisfatória da claudicação. A técnica modificada demonstrou ser eficaz para o nivelamento do platô tibial, inibindo o deslocamento cranial da tíbia, proporcionando 100% de melhora nos animais operados, com mínimas complicações e encurtamento tibial, bem como muita satisfação dos respectivos proprietários. Além disso, conferiu espaço suficiente para estabilização com implantes utilizando placa para TPLO com bloqueio e estabilização auxiliar com banda de tensão. O planejamento através do programa digital Veterinary Preoperative Orthopaedic Planning (vPOP), o alinhamento dos fragmentos pelas corticais craniais, o posicionamento proximal da cunha com base na localização do CORA respeitando-se a distância para a osteotomia da linha proximal da cunha, o uso de placa de TPLO bloqueada e com compressão dinâmica, o posicionamento da cunha e da placa conforme distâncias denominadas de D1, D2, D3, D4 e D5 pré-estabelecidas no planejamento e a complementação da estabilização com pino cranial em banda de tensão parecem ser parâmetros importantes a serem respeitados para se atingir o sucesso e recuperação funcional. Sugere-se avaliação da técnica em maior tempo e números de animais.

In view of the high frequency of cranial cruciate ligament rupture (CrCLR) in dogs, the cranial tibial wedge ostectomy (CTWO) is one of the osteotomy techniques to reduce the angle of the tibial plateau (TPA) and thus cancel the cranial displacement of the proximal tibia in relation to the femoral condyles. The study aimed to use the center of rotation of angulation (CORA) method to position the CTWO wedge, first performing a graphic evaluation of the proposal and then applying the results to dogs affected with CrCLR. After determining the new method, analysis was performed on 19 radiographic images of the tibia of normal adult dogs to verify changes in the TPA and tibial shortening with this new technique. Subsequently, nine knees of seven dogs with CrCLR were operated, where the tibial fragments, proximal and distal, were fixed with a blocked plate and dynamic compression of TPLO and tension band, where the wedge was never less than 5 mm distal to the insertion of the patellar ligament, and in all cases, the fragments were aligned with the cranial cortex. He application of the technique allowed a reduction in the average TPA from 24.04 ° to 7.74 °. It can be seen that the mean of the TPA according to the alignment of the fragments varied from 7.73 to 8.03 °, with no significant difference between the three categories of alignment. TPA averages according to the height of the wedge ranged from 7.26 ° (5 mm below the insertion of the patellar ligament), to 8.47 ° (at the height of the distal) and was 7.74 ° when the height was in the CORA and the difference is significant. The mean bone shortening of 0.16 cm, which corresponds to the functional axis not considered significant to influence clinical recovery. Minor complications such as fragment fracture, wound dehiscence and traditional meniscus injury occurred in trhee animals without impairing functional recovery, with satisfactory change in lameness. The modified technique proved to be effective for leveling the plateau and inhibiting displacement cranial tibia, offering improvement in 100% of the dogs with minimal complications and tibial shortening, and with great satisfaction from the respective owners In addition, it provided enough space for stabilization with implants using plate with TPLO lock and auxiliary stabilization with tension band. The planning through the digital program Veterinary Preoperative Orthopedic Planning (vPOP), the alignment of the fragments by the cranial cortices, the proximal positioning of the wedge based on the CORA's location respecting 5mm of distance to the osteotomy of the proximal wedge line, the use of blocked TPLO plate and with dynamic compression, the wedge and plate positioning according to distances called D1, D2, D3, D4 and D5 pre-established in the planning and the complementation of the stabilization with cranial pin in tension band seem to be important parameters to be respected to achieve success and functional recovery. It is recommended to evaluate the technique in a longer time and larger numbers of animals.