Long-term consumption of recycled cooking oil induces cell death and tissue damage.

Recycled cooking oil (RCO) is widely used in many small restaurants. However, the health risk posed by long-term consumption of RCO is unclear. In this study, C57 mice were treated with RCO for 34 weeks. Organ coefficients of the liver, stomach, and kidney were found to be decreased. H&E staining revealed overt lesions in the pancreas, liver, kidney, esophagus, duodenum, and ileum of RCO-treated mice. Immunohistochemistry showed significant DNA damage in the duodenum and ileum and apoptosis in the lungs of the RCO-treated mice. Immunoblotting showed elevated levels of γ-H2AX, Bcl-2/Bax, TNFα, cleaved Caspase-3 and poly ADP-ribose polymerase (PARP). Increased levels of lactate dehydrogenase (LDH) and decreased levels of succinate dehydrogenase (SDH) were also detected. These findings suggest that long-term consumption of RCO produces various toxicities in mice with important implications for humans. DNA damage followed by mitochondria-associated apoptosis, and necrosis is likely to contribute to the toxicities.

Impact of kitchen organization on oral intake of malnourished inpatients: A two-center study / Impacto de la organización de la cocina hospitalaria en la ingesta del paciente malnutrido: estudio de dos centros

Aim: To determine the impact of the type of hospital kitchen on the dietary intake of patients. Methods: A cross-sectional, two-centre study, of cooking in a traditional kitchen (TK) and in a chilled kitchen (CK). Subjective global assessment (SGA) was used for nutritional diagnosis. Before study start, a dietician performed a nutritional assessment of the menus of each hospital. All dishes were weighed upon arrival to the ward and at the end of the meal. Results: 201 and 41 patients from the centres with TK and CK respectively were evaluated. Prevalence of malnutrition risk was 50.2% at the hospital with TK and 48.8% at the hospital with CK (p=0.328). Forty-eight and 56 dishes were nutritionally evaluated at the hospitals with TK and CK respectively. Intake analysis consisted of 1993 and 846 evaluations in the hospitals with TK and CK respectively. Median food consumption was 76.83% at the hospital with TK (IQR 45.76%) and 83.43% (IQR 40.49%) at the hospital with CK (p<0.001). Based on the prevalence of malnutrition, a higher protein and energy intake was seen in malnourished patients from the CK as compared to the TK hospital, but differences were not significant after adjustment for other factors. Conclusions: Cooking in a chilled kitchen, as compared to a traditional kitchen, may increase energy and protein intake in hospitalized patients, which is particularly beneficial for malnourished patients (AU)

Objetivo: Determinar el impacto de la organización de la cocina hospitalaria en la ingesta dietética del paciente hospitalizado. Metodología: Estudio transversal, realizado en dos centros hospitalarios, uno con cocina tradicional (CT) y otro con cocina en línea fría (CLF). La valoración subjetiva global fue empleada para el diagnóstico nutricional. Una dietista-nutricionista realizó una calibración nutricional de los platos y los menús de cada hospital antes de empezar el estudio. La técnica de valoración de la ingesta fue la pesada de alimentos antes y después de la ingesta, siempre en presencia del paciente. Resultados: Fueron reclutados 201 pacientes del centro con CT y 41 del CLF. La prevalencia de riesgo de desnutrición fue del 50,2% en el CT y de 48,8% en el CLF (p=0,328). En el CT fueron valorados nutricionalmente 48 platos y 56 del CLF. Respecto al análisis de la ingesta, se realizaron 1.993 registros en el centro CT y 846 en el centro CLF. La mediana de ingesta en el CT fue de 76,83% (RIC 45,76%) y 83,43% (RIC 40,49%) en el CLF (p<0,001). Teniendo en cuenta la prevalencia de malnutrición, se observó una mayor ingesta proteica y energética en pacientes malnutridos en el CLF en comparación con el CT, aunque estas diferencias no fueron significativas tras ajustarlas a diferentes factores de confusión. Conclusiones: Cocinar en una cocina en línea fría podría mejorar la ingesta calórica y proteica del paciente hospitalizado, especialmente en pacientes malnutridos (AU)

How the variance of some extraction variables may affect the quality of espresso coffees served in coffee shops.

BACKGROUND: To improve the quality of espresso coffee, the variables under the control of the barista, such as grinding grade, coffee quantity and pressure applied to the coffee cake, as well as their variance, are of great importance. A nonlinear mixed effect modeling was used to obtain information on the changes in chemical attributes of espresso coffee (EC) as a function of the variability of extraction conditions. RESULTS: During extraction, the changes in volume were well described by a logistic model, whereas the chemical attributes were better fit by a first-order kinetic. The major source of information was contained in the grinding grade, which accounted for 87-96% of the variance of the experimental data. The variability of the grinding produced changes in caffeine content in the range of 80.03 mg and 130.36 mg when using a constant grinding grade of 6.5. CONCLUSION: The variability in volume and chemical attributes of EC is large. Grinding had the most important effect as the variability in particle size distribution observed for each grinding level had a profound effect on the quality of EC. Standardization of grinding would be of crucial importance for obtaining all espresso coffees with a high quality. © 2015 Society of Chemical Industry.

Effect of dietary fiber on properties and acceptance of meat products: a review.

Meat is an important source of all essential nutritional components of our daily diet as it content most of the essential amino acids, fatty acids, vitamins, and minerals which are lack in plant based food, but it is devoid of dietary fiber, which is very essential component for normal physiological/biochemical process. During meat products processing, its functional values can be improved by supplementation of dietary fiber rich vegetative substances like cereal and pulse flour, vegetable and fruits pulp, etc. by this process, a significant proportion of required daily allowance of dietary fiber can be fulfilled for the frequent meat consumers. The consumption of meat products fortified with of dietary fiber can lead to the prevention of diseases like coronary heart disease, diabetes, irritable bowel disease, obesity, etc. On the other hand, the dietary fiber can effectively be incorporated in the processed meat products as binders, extender, and filler, they can significantly replace the unhealthy fat components from the products; increase acceptability by improving nutritional components, pH, water-holding capacity, emulsion stability, shear press value, sensory characters, etc. of finished products. Addition of dietary fiber in the meat products can increase the cooking yield therefore the economic gain as well.

Influence of dextran-producing Weissella cibaria on baking properties and sensory profile of gluten-free and wheat breads.

Breads based on gluten-free buckwheat, quinoa, sorghum and teff flours were produced with addition of 20% sourdough fermented with exopolysaccharide (EPS) producing Weissella cibaria MG1. Wheat bread was baked as a reference. Dough rheology, bread quality parameters and sensory properties of the sourdough-containing breads were compared to sourdough non-containing control breads of the respective flour. The specific volume remained unaffected by sourdough application. In buckwheat, sorghum, teff and wheat sourdough breads acidification increased crumb porosity compared to control breads. Crumb hardness was significantly reduced in buckwheat (-122%), teff (-29%), quinoa (-21%) and wheat sourdough breads (-122%). The staling rate was significantly reduced in buckwheat, teff and wheat sourdough breads. Water activity of the sourdough containing bread crumb was not influenced by the presence of EPS. Due to the presence of exopolysaccharides (EPS) and influence of acidification, the dough strength, AF, as measured by oscillation tests decreased significantly in sourdough-containing buckwheat, sorghum and wheat dough, but increased in sourdough-containing quinoa and teff dough. Microbial shelf-life was significantly prolonged neither for gluten-free sourdough nor for wheat sourdough breads. Scanning electron microscopy of control and sourdough bread crumbs did not show differences concerning structural starch features. In addition, the aroma of most bread was not improved by sourdough addition.

Standardization of domestic frying processes by an engineering approach.

An approach was developed to enable a better standardization of domestic frying of potato products. For this purpose, 5 domestic fryers differing in heating power and oil capacity were used. A very defined frying process using a highly standardized model product and a broad range of frying conditions was carried out in these fryers and the development of browning representing an important quality parameter was measured. Product-to-oil ratio, oil temperature, and frying time were varied. Quite different color changes were measured in the different fryers although the same frying process parameters were applied. The specific energy consumption for water evaporation (spECWE) during frying related to product amount was determined for all frying processes to define an engineering parameter for characterizing the frying process. A quasi-linear regression approach was applied to calculate this parameter from frying process settings and fryer properties. The high significance of the regression coefficients and a coefficient of determination close to unity confirmed the suitability of this approach. Based on this regression equation, curves for standard frying conditions (SFC curves) were calculated which describe the frying conditions required to obtain the same level of spECWE in the different domestic fryers. Comparison of browning results from the different fryers operated at conditions near the SFC curves confirmed the applicability of the approach.

Excess sodium and insufficient iron content in complementary foods.

OBJECTIVES: To determine, by chemical analysis, the macronutrient, energy, sodium, and iron contents of homemade foods prepared for infants in two socioeconomic classes in Belém, state of Pará, Brazil. METHODS: Cross-sectional study of 78 infants (aged 6 to 18 months) distributed into two groups according to socioeconomic status (high or low). Chemical analyses were performed on samples of homemade complementary foods prepared for each infant's lunch. Daily food intake was estimated on the basis of two 24-hour dietary intake recall. RESULTS: Chemical analyses showed that the energy content of some food samples was lower than recommended, both in the low socioeconomic status (SES) group (29.8% of samples) and in the high-SES group (43.0%; p = 0.199). The iron content of all samples, regardless of group, was lower than minimum recommended levels (6.0 mg/100 g). On the other hand, excessive sodium levels (200 mg/100 g) were found in 89.2 and 31.7% of samples in the low- and high-SES groups, respectively (p = 0.027). Dietary recalls showed that energy intake exceeded 120% of the Estimated Energy Requirement in 86.5% of infants in the low-SES group and 92.7% of those in the high-SES group (p = 0.483). Lunch and dinner provided 35.2+/-14.6 and 36.4+/-12.0% of daily energy intake in the low- and high-SES groups, respectively (p = 0.692). CONCLUSION: Homemade complementary foods for infants were found to be low in iron. A significant portion of samples had excessive sodium content, most frequently those prepared for infants in low-SES status families.

Excesso de sódio e déficit de ferro em alimentos de transição / Excess sodium and insufficient iron content in complementary foods

OBJETIVOS: Determinar, por análise química, a composição nutricional de macronutrientes, energia, sódio e ferro de alimentos preparados no domicílio para lactentes de dois estratos socioeconômicos em Belém (PA). MÉTODOS: Estudo transversal com 78 lactentes (6 a 18 meses) distribuídos em dois grupos com condição socioeconômica alta ou baixa. Foi realizada análise química de amostras de alimentos de transição preparados no domicílio para o almoço. Foi estimada a ingestão alimentar diária, com base em dois inquéritos alimentares de 24 horas. RESULTADOS: As análises químicas revelaram que parcela das amostras dos alimentos apresentava baixo teor de energia em relação ao recomendado, tanto no estrato socioeconômico baixo (29,8 por cento) como no alto (43,0 por cento; p = 0,199). Todas as amostras analisadas, em ambos os grupos, apresentaram quantidade de ferro abaixo do mínimo recomendado (6,0 mg/100 g). Por outro lado, excesso de sódio (200 mg/100 g) foi constatado em 89,2 e 31,7 por cento, respectivamente, das amostras dos grupos de baixo e alto nível socioeconômico (p = 0,027). De acordo com os inquéritos alimentares, a estimativa da ingestão energética foi maior que 120 por cento da necessidade média estimada em 86,5 por cento dos lactentes do grupo de nível socioeconômico baixo e em 92,7 por cento do alto (p = 0,483). O almoço e o jantar forneceram 35,2±14,6 e 36,4±12,0 por cento da energia, respectivamente, nos grupos de baixo e alto nível socioeconômico (p = 0,692). CONCLUSÃO: Alimentos de transição preparados no domicílio para lactentes apresentaram baixo teor de ferro. Parcela expressiva das amostras apresentou quantidade excessiva de sódio, mais frequentemente nos alimentos preparados para os lactentes de baixo nível socioeconômico.

OBJECTIVES: To determine, by chemical analysis, the macronutrient, energy, sodium, and iron contents of homemade foods prepared for infants in two socioeconomic classes in Belém, state of Pará, Brazil. METHODS: Cross-sectional study of 78 infants (aged 6 to 18 months) distributed into two groups according to socioeconomic status (high or low). Chemical analyses were performed on samples of homemade complementary foods prepared for each infant's lunch. Daily food intake was estimated on the basis of two 24-hour dietary intake recall. RESULTS: Chemical analyses showed that the energy content of some food samples was lower than recommended, both in the low socioeconomic status (SES) group (29.8 percent of samples) and in the high-SES group (43.0 percent; p = 0.199). The iron content of all samples, regardless of group, was lower than minimum recommended levels (6.0 mg/100 g). On the other hand, excessive sodium levels (200 mg/100 g) were found in 89.2 and 31.7 percent of samples in the low- and high-SES groups, respectively (p = 0.027). Dietary recalls showed that energy intake exceeded 120 percent of the Estimated Energy Requirement in 86.5 percent of infants in the low-SES group and 92.7 percent of those in the high-SES group (p = 0.483). Lunch and dinner provided 35.2±14.6 and 36.4±12.0 percent of daily energy intake in the low- and high-SES groups, respectively (p = 0.692). CONCLUSION: Homemade complementary foods for infants were found to be low in iron. A significant portion of samples had excessive sodium content, most frequently those prepared for infants in low-SES status families.

Near infrared spectroscopy: an analytical tool to predict coffee roasting degree.

The main purpose of this study was to investigate the relationship between some coffee roasting variables (weight loss, density and moisture) with near infrared (NIR) spectra of original green (i.e. raw) and differently roasted coffee samples, in order to test the availability of non-destructive NIR technique to predict coffee roasting degree. Separate calibration and validation models, based on partial least square (PLS) regression, correlating NIR spectral data of 168 representatives and suitable green and roasted coffee samples with each roasting variable, were developed. Using PLS regression, a prediction of the three modelled roasting responses was performed. High accuracy results were obtained, whose root mean square errors of the residuals in prediction (RMSEP) ranged from 0.02 to 1.23%. Obtained data allowed to construct robust and reliable models for the prediction of roasting variables of unknown roasted coffee samples, considering that measured vs. predicted values showed high correlation coefficients (r from 0.92 to 0.98). Results provided by calibration models proposed were comparable in terms of accuracy to the conventional analyses, revealing a promising feasibility of NIR methodology for on-line or routine applications to predict and/or control coffee roasting degree via NIR spectra.

Food oxalate: factors affecting measurement, biological variation, and bioavailability.

Food and nutrition professionals provide medical nutrition therapy for patients with kidney stones. If the stones contain oxalate or the patient has been diagnosed with hyperoxaluria, reduction of dietary oxalate may be appropriate. Differences in oxalate values for a single food may be due to analytical methods, and/or biological variation from several sources, including cultivar, time of harvest, and growing conditions. Bioavailability of food oxalate and, thus, urine oxalate, will also be affected by salt forms of oxalate, food processing and cooking methods, meal composition, and the presence of Oxalabacter formigenes in the patient's gut. Dietary advice for reducing urinary oxalate should include both reduction of dietary oxalate and simultaneous consumption of calcium-rich food or supplement to reduce oxalate absorption.

Technological developments and the need for technical competencies in food services.

The growing scale of institutional and commercial food services poses a technological challenge of producing large quantities of high quality meals in terms of their safety, sensory and nutritional attributes. Developments in food service technology and systems (cook-freeze, cook-chill and others) allow the replacement of fast food with the service of cooked meals, which are often nutritionally superior. Reliance on equipment, packaging and technological 'know-how' makes food service operations more complex. Operators have to minimise the impact of the numerous steps in the production process, the fundamental weaknesses of cook-chill food safety design, coupled with the practical limitations of Hazard Analysis Critical Control Points management, the potential unevenness of temperature distribution and product deterioration during storage. The fundamental knowledge of food science and microbiology, engineering and packaging technologies is needed. At present, the 'high tech' options, which can improve a product's nutritional value, such as natural preservation hurdles or functional meals, are not used in practice.

Laser mass spectrometry as on-line sensor for industrial process analysis: process control of coffee roasting.

The objective of the project is to develop on-line, real-time, and noninvasive process control tools of coffee roasting that help deliver a consistent and high-quality coffee aroma. The coffee roasting process was analyzed by direct injection of the roaster gas into a time-of-flight mass spectrometer and ionized either by resonance enhanced multiphoton ionization (REMPI) at 266 and 248 nm or vacuum ultraviolet single-photon ionization (VUV-SPI) at 118 nm. The VUV ionization scheme allows detecting mainly the most volatile and abundant compounds of molecular mass below 100 m/z, while REMPI ionizes mainly aromatic compounds of molecular mass larger than 100 m/z. Combining the compounds ionized by resonant and single-photon ionization, approximately 30 volatile organic compounds are monitored in real time. Time-intensity profiles of 10 important volatile coffee compounds were discussed in connection with their formation chemistry during roasting. Applying multivariate statistics (principle component analysis) on time-intensity traces of nine volatile coffee compounds, the roasting degree could be traced as a consistent path in the score plot of the two most significant principle components (including 68% of the total variance), for a range of roasting temperatures (200-250 degrees C).

Drink boiled water: a cultural analysis of a health education message.

Water boiling is recommended by health educators in Sri Lanka, and boiled water is given to ill and vulnerable people, but it is not widely consumed by the public. The reasons for this behavior derive from long-standing notions about health care. This study complements one presented some years ago by Wellin, based on the health culture of Peruvians.